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Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson's Disease
Jang, Jihoon,Jeong, Soyeon,Lee, Sung Ik,Seol, Wongi,Seo, Hyemyung,Son, Ilhong,Ho, Dong Hwan Hindawi 2018 Parkinson’s disease Vol.2018 No.-
<P>Parkinson's disease (PD) is the second most common neurodegenerative disease. Oxidative stress is the most critical risk factor for neurodegenerative diseases, including Alzheimer's disease (AD) and Huntington's disease (HD). Numerous reports have demonstrated that oxidative stress aggravates cytotoxicity in dopaminergic neurons and accelerates the formation of protein inclusions. In addition, oxidative stress, such as 4-hydroxynonenal (HNE), oxidized protein, and dopamine quinone, are related to PD progression. <I>DJ-1</I> is a PD-causative gene, and it plays a pivotal role as a sensor and eliminator of oxidative stress. Several studies have shown that oxidized DJ-1 (OxiDJ-1) formation is induced by oxidative stress. Hence, previous studies suggest that oxidized DJ-1 could be a biomarker for PD. We previously reported higher DJ-1 levels in Korean male PD patient urine exosomes than male non-PD controls. We speculate that OxiDJ-1 levels in PD patient urine might be higher than that in non-PD controls. In this study, we established an ELISA for OxiDJ-1 using recombinant DJ-1 treated with H<SUB>2</SUB>O<SUB>2</SUB>. Using Western blot assay and ELISA, we confirmed an increase of OxiDJ-1 from HEK293T cells treated with H<SUB>2</SUB>O<SUB>2</SUB>. Using our ELISA, we observed significantly higher, 2-fold, OxiDJ-1 levels in the urine of Korean PD patients than in non-PD controls.</P>
Jang, Jihoon,Kim, Jung-Keun,Choi, Jin-Woo,Hwang, Taek-Sung,Jo, Mijung,Kim, Inhye,Cho, Byoung-Ki,Lee, Eunji The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.73
<P>The amphiphilic block codendrimer self-assembled into supramolecular vesicles with a bilayer membrane in both hydrophilic and hydrophobic solvents. Surface-modified quantum dots (QDs) were successfully entrapped within the wall of vesicles through a simple self-assembly process without any structural disruption.</P> <P>Graphic Abstract</P><P>A Janus amphiphilic codendrimer can lead to the formation of organic–inorganic hybrid hollow capsules by co-assembly with surface-modified quantum dots in both hydrophilic and hydrophobic solvents. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc42213e'> </P>
Jang, Jihoon,Oh, Hakjin,Nam, Daleum,Seol, Wongi,Seo, Mi Kyoung,Park, Sung Woo,Kim, Hyung Gun,Seo, Hyemyung,Son, Ilhong,Ho, Dong Hwan ZOOLOGICAL SOCIETY OF KOREA 2018 ANIMAL CELLS AND SYSTEMS Vol.22 No.5
<P><B>ABSTRACT</B></P><P>Leucine-rich repeat kinase 2 (LRRK2) is involved in Parkinson’s disease (PD) pathology. A previous study showed that rotenone treatment induced apoptosis, mitochondrial damage, and nucleolar disruption via up-regulated LRRK2 kinase activity, and these effects were rescued by an LRRK2 kinase inhibitor. Heat-shock protein 70 (Hsp70) is an anti-oxidative stress chaperone, and overexpression of Hsp70 enhanced tolerance to rotenone. Nucleolin (NCL) is a component of the nucleolus; overexpression of NCL reduced cellular vulnerability to rotenone. Thus, we hypothesized that rotenone-induced LRRK2 activity would promote changes in neuronal Hsp70 and NCL expressions. Moreover, LRRK2 G2019S, the most prevalent LRRK2 pathogenic mutant with increased kinase activity, could induce changes in Hsp70 and NCL expression. Rotenone treatment of differentiated SH-SY5Y (dSY5Y) cells increased LRKK2 levels and kinase activity, including phospho-S935-LRRK2, phospho-S1292-LRRK2, and the phospho-moesin/moesin ratio, in a dose-dependent manner. Neuronal toxicity and the elevation of cleaved poly (ADP-ribose) polymerase, NCL, and Hsp70 were increased by rotenone. To validate the induction of NCL and Hsp70 expression in response to rotenone, cycloheximide (CHX), a protein synthesis blocker, was administered with rotenone. Post-rotenone increased NCL and Hsp70 expression was repressed by CHX; whereas, rotenone-induced kinase activity and apoptotic toxicity remained unchanged. Transient expression of G2019S in dSY5Y increased the NCL and Hsp70 levels, while administration of a kinase inhibitor diminished these changes. Similar results were observed in rat primary neurons after rotenone treatment or G2019S transfection. Brains from G2019S-transgenic mice also showed increased NCL and Hsp70 levels. Accordingly, LRRK2 kinase inhibition might prevent oxidative stress-mediated PD progression.</P><P><B>Abbreviations:</B> 6-OHDA: 6-hydroxydopamine; CHX: cycloheximide; dSY5Y: differentiated SH-SY5Y; g2019S tg: g2019S transgenic mouse; GSK/A-KI: GSK2578215A kinase inhibitor; HSP70: heat shock protein 70; LDH: lactose dehydrogenase; LRRK2: leucine rich-repeat kinase 2; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; myc-GS LRRK2: myc-tagged g2019S LRRK2; NCL: nucleolin; PARP: poly(ADP-ribose) polymerase; PD: Parkinson’s disease; PINK1: PTEN-induced putative kinase 1; pmoesin: phosphorylated moesin at t558; ROS: reactive oxygen species</P>
Pseudo-renal Failure Caused by Urinary Bladder Rupture in Multiple Trauma Patient
( Jihoon Jang ),( Kyoung Hoon Lim ) 대한외상학회 2016 大韓外傷學會誌 Vol.29 No.4
Pseudo-renal failure presents with renal failure characteristics, such as hypercreatininemia and hyperkalemia without a change in glomerular filtration rate or structure of the kidney. Pseudo-renal failure due to trauma is difficult to diagnose, because symptoms are non-specific and other factors may cause hypercreatininemia and hyperkalemia. In a trauma patient, especially one with pelvic injury, the abrupt elevation of potassium, blood urea nitrogen, and creatinine levels without previous medical history is a key feature in the diagnosis of urinary ascites. We report a case of pseudo-renal failure caused by intraperitoneal bladder rupture in a multiple trauma patient. [ J Trauma Inj 2016; 29: 191-194 ]
Forearm Replantation for a Patient Presented with Major Amputation Injury: A Case Report
( Jihoon Jang ),( Kyoung Hoon Lim ),( Joon-woo Kim ),( Hyung-kee Kim ) 대한외상학회 2016 大韓外傷學會誌 Vol.29 No.4
With the development of safety measures for employees who work with dangerous machinery, the frequency of amputation injuries has been decreasing with resultant decrease in replantation procedures. However, in some patients with major amputation injury, replantation is still necessary for the preservation of limb and it s function. The replantation of the upper extremity (UE) is a complex and technically demanding surgical procedure. For the successful replantation of UE, the type of injury, reconstruction sequence, ischemic time, and other combined injury of patient should be considered. We report a case of major amputation of UE by guillotine-type injury and discuss the treatment process of this patient. [ J Trauma Inj 2016; 29: 187-190 ]
나노 임프린트 공정을 이용한 Ag 나노로드 제조 및 비정질 박막 태양전지 적용
장지훈(Jang, JiHoon),한강수(Han, Kang-Soo),조준식(Cho, Jun-Sik),이헌(Lee, Heon),박해웅(Park, Hai Woong),송진수(Song, Jinsoo),이정철(Lee, Jeong Chul) 한국신재생에너지학회 2011 한국신재생에너지학회 학술대회논문집 Vol.2011 No.05
비정질 실리콘 태양전지의 효율을 증가하기 위하여 많이 사용되는 방법 중 하나는 입사되는 빛의 산란을 증가하여 태양전지의 광흡수를 증가시키는 방법이다. 이를 위하여 양극전극으로 사용되는 TCO층의 일정한 패턴 처리를 통하여 광산란을 증가시키는 방법이 사용되고 있다. 본 연구에서는 나노 임프린트 리소그래피방법을 사용하여 Ag 나노로드를 증착한 기판을 제조하고 이를 비정질 실리콘 태양전지에 적용하였다. 실험결과, 그림과 같이 높이와 너비가 300nm 정도로 일정한 패턴의 Ag 나노로드를 제조하였다. 또한, 그 위에 증착된 Si 박막의 경우, 나노로드 전체를 감싸는 돔 형태로 성장하였다. 이와 같은 나노로드 위에 substrate n-i-p 구조의 비정질 박막 태양전지를 증착하고 그 특성변화를 분석하고자 하였다.
R600a를 이용한 소형 멀티형 냉장고 시스템의 성능특성에 대한 실험적 연구
안지훈(Jihoon Ahn),장용희(Yonghee Jang),김용찬(Yongchan Kim),김용한(Yonghan Kim),장의영(Uiyoung Jang),박용종(Yongjong Park) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
Various types of refrigerators become popular in the market such as a common refrigerator, kimchi refrigerator and wine cellar. It is required to develop a multi function refrigerator which has different purposes in each evaporator. In this study, the performance of a multi type refrigerator system, which consists of one machine room and three evaporators, was measured in a bench type multi refrigerator. The multi type refrigerator system was tested by varying the number of refrigerator cabinet, refrigerant charge amount, compressor frequency and temperature condition. Based on experimental data, the multi type refrigerator showed better performance than the conventional refrigerator (single type). At the same external load condition, the COP of the multi type refrigerator was 1.2~1.5, but the COP of the single type refrigerator was 0.8. The single and multi type refrigerator cycles were optimized at -30.0℃ and -25.0℃, respectively. Therefore, the multi type refrigerator may show low compression ratio than the single type refrigerator.