A pilot study of differential brain activation to suicidal means and DNA methylation of CACNA1C gene in suicidal attempt patients

Kim, Young Jong,Park, Hae Jeong,Jahng, Geon-Ho,Lee, Sang Min,Kang, Won Sub,Kim, Su Kang,Kim, Tae,Cho, Ah Rang,Park, Jin Kyung Elsevier 2017 Psychiatry Research Vol.255 No.-

<P><B>Abstract</B></P> <P>Suicide is a serious public health problem. In this study, we investigated functional brain changes to novel visual stimuli of suicidal means, DNA methylation status, and the relationship between the two markers. 14 suicidal attempt patients (SAs) and 22 healthy controls were included. Pictures of facial expressions and suicidal means were shown to subjects during fMRI scanning. 11 CpG sites within transcription factor binding site of CACNA1C gene were selected. In knives (K) vs. natural landscape (NL) condition, left middle frontal gyrus (Brodmann's area, BA 6 and 46) and left inferior frontal gyrus (BA 9) were shown to be significantly higher brain activation in the SAs than the controls (p<0.001). DNA methylation percentages of CpG site 4 (p=0.005) and 6 (p=0.037) were found to be related to the SAs. In the SAs, methylation degree of site 4 and site 6 was positively correlated with signal intensity of K vs. NL condition in left thalamus. The degree of site 4 was positively correlated with signal intensity in left middle and inferior frontal gyri in SAs. The possibility that these findings might be involved in the neurobiology of suicidal behavior is suggested.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Functional brain change and DNA methylation were investigated in suicidal attempters. </LI> <LI> Distinct brain activation and DNA methylation were found in suicidal attempters. </LI> <LI> The possibility of a neurobiological evidence for suicide is suggested. </LI> </UL> </P>

건강 자원자에서 위 배출에 미치는 tadalafil의 효과

김민수 ( Min Su Kim ),장진혁 ( Jin Hyuck Chang ),김익성 ( Ek Seong Kim ),황성준 ( Seoung Joon Hwang ),장재훈 ( Jae Hoon Jahng ),김승민 ( Seung Min Kim ),유영훈 ( Young Hoon Ryu ),박효진 ( Hyo Jin Park ) 대한소화기기능성질환·운동학회 2006 Journal of Neurogastroenterology and Motility (JNM Vol.12 No.1

목적: 본 교실에서는 건강 자원자에서 phosphodiesterase (PDE) 5형 억제제인 sildenafil이 전체 위 배출능에는 영향이 없었으나 음식물의 위내 분포에는 영향이 있음을 보고한 적이 있다. 이에 sildenafil 보다 작용시간이 긴 것으로 알려진 tadalafil (cialis(R))은 위 배출에 어떠한 영향을 미치는지 알아보고자 하였다. 대상 및 방법: 건강한 남자 자원자 8명(평균 나이 26.4세)을 대상으로 투약 전, tadalafil 투약 30분 후, 투약 24시간 후에 위 배출 주사를 시행하여 전체 위 배출능을 알아보기 위한 지표로서 total T(1/2) (min)을 구하였고, 위내 분포를 알아보기 위한 지표 중 위 근위부에 대해서는 proximal T(1/2) (min)과 검사식 섭취 직후 위 근위부의 방사선량인 initial proximal activity (%)를, 위 원위부에 대해서는 위 원위부에서 가장 높이 기록된 방사선량인 maximal distal content (%)을 구한 다음 tadalafil 효과 및 24시간 후까지 작용 지속 여부를 확인하였다. 결과: 위 배출 주사 결과 투약 전, tadalafil 투약 30분 후, 투약 24시간 후의 total T(1/2) (min), proximal T(1/2) (min), initial proximal activity (%), maximal distal content (%)가 모두 통계학적으로 유의한 차이를 보이지 않았다. 결론: 최근 시판된 PDE 5형 억제제, tadalafil은 위 배출 및 위내 분포에 유의한 영향을 미치지 않음을 확인할 수 있었다. Background/Aims: In a previous study we demonstrated an influence of sildenafil, a phosphodiesterase (PDE) type 5 inhibitor, on intragastric food maldistribution without affecting total gastric emptying. In this we wanted to determine the effect of tadalafil (cialis(R)), which has longer duration of action than sildenafil, on gastric emptying. Methods: Eight male (mean age, 26.4 yrs) healthy volunteers were selected. Total T(1/2) (min) was measured to assess total gastric emptying before, 30 minutes and 24 hours after tadalafil intake. Proximal T(1/2) (min), initial proximal activity (%)-radioactiviy measured in the proximal body of the stomach immediately after food ingestion-, and the maximal distal content (%)-the highest radioactiviy measured in the distal body of the stomach was measured as indicators of intragastric food distribution. Results: There were no significant differences in total T(1/2) (min), proximal T(1/2) (min), initial proximal activity (%) and maximal distal content (%) before and 30 minutes and 24 hrs after intake of tadalafil. Conclusions: The recently launched PDE5 inhibitor, tadalafil, has no significant influence on total gastric emptying or intragastric food distribution. (Kor J Neurogastroenterol Motil 2006;12:31-34)

Histamine H1 receptor induces cytosolic calcium increase and aquaporin translocation in human salivary gland cells.

Kim, Ji-Hyun,Park, Seong-Hae,Moon, Young Wha,Hwang, Sungmin,Kim, Donghoon,Jo, Su-Hyun,Oh, Seog Bae,Kim, Joong Soo,Jahng, Jeong Won,Lee, Jong-Ho,Lee, Sung Joong,Choi, Se-Young,Park, Kyungpyo Williams Wilkins 2009 The Journal of pharmacology and experimental thera Vol.330 No.2

<P>One of the common side effects of antihistamine medicines is xerostomia (dry mouth). The current consensus is that antihistamine-induced xerostomia comes from an antimuscarinic effect. Although the effect of antihistamines on salivary secretion is both obvious and significant, the cellular mechanism whereby this happens is still unclear because of the lack of knowledge of histamine signaling in human salivary glands. Here, we have studied histamine receptors and the effect of antihistamines on human submandibular acinar cells. In primary cultured human submandibular gland and a HSG cell line, histamine increased the intracellular Ca(2+) concentration. The histamine-induced cytosolic free Ca(2+) concentration ([Ca(2+)](i)) increase was inhibited by histamine H1 receptor-specific antagonists, and the expression of the functional histamine H1 receptor was confirmed by reverse transcription-polymerase chain reaction. Interestingly, histamine pretreatment did not inhibit a subsequent carbachol-induced [Ca(2+)](i) rise without 'heterologous desensitization.' Chlorpheniramine inhibited a carbachol-induced [Ca(2+)](i) increase at a 100-fold greater concentration than histamine receptor antagonism, whereas astemizole and cetrizine showed more than 1000-fold difference, which in part explains the xerostomia-inducing potency among the antihistamines. Notably, histamine resulted in translocation of aquaporin-5 to the plasma membrane in human submandibular gland cells and green fluorescent protein-tagged aquaporin-5 expressing HSG cells. We found that histidine decarboxylase and the histamine H1 receptor are broadly distributed in submandibular gland cells, whereas choline acetyltransferase is localized only at the parasympathetic terminals. Our results suggest that human salivary gland cells express histamine H1 receptors and histamine-synthesizing enzymes, revealing the cellular mechanism of antihistamine-induced xerostomia.</P>

High-sensitivity C-reactive protein and mean platelet volume as predictive values after percutaneous coronary intervention for long-term clinical outcomes: a comparable and additive study

Moon, Ae Ran,Choi, Dong-Hyun,Jahng, Su-Young,Kim, Bo-Bae,Seo, Hong-Joo,Kim, Sang Hun,Ryu, Sang-Wan,Song, Heesang,Kim, Tae-Hyoung YEAR Wolters Kluwer Health, Inc. All rights reserv 2016 Blood coagulation & fibrinolysis Vol.27 No.1

<P>This study was designed to establish the relationship of high-sensitivity C-reactive protein (hsCRP) and mean platelet volume (MPV) with the development of adverse outcomes after percutaneous coronary intervention (PCI). hsCRP levels and MPV were analysed in 372 patients who underwent PCI, with the primary endpoint as major adverse cardiac and cerebrovascular events (MACCE): a composite of cardiac death, myocardial infarction (MI), target vessel revascularization (TVR), ischemic stroke and stent thrombosis. During the follow-up period (mean, 25.8 months), there were 21 cardiac deaths, 10 MIs including four stent thrombosis events, seven ischemic strokes and 29 TVRs. The hsCRP cut-off level was set at 0.31mg/dl using the receiver operating characteristic curve to differentiate between the groups with and without MACCE. The MPV cut-off level was set at 8.00fl by the receiver operating characteristic curve to differentiate between the groups with and without MACCE. A Kaplan-Meier analysis revealed that the high hsCRP group (0.31mg/dl) had a significantly higher cardiac death and MACCE rate than the low hsCRP group (<0.31mg/dl), and the high MPV group (>8.00fl) had a significantly higher cardiac death and MACCE rate than the low MPV group (8.00fl). Furthermore, the high hsCRP and MPV groups were significantly associated with an increased risk of MACCE. These results show that hsCRP and MPV are predictive markers after PCI for MACCE; they are also additively associated with a higher risk of MACCE. Blood Coagul Fibrinolysis 27: 70-76 Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved.</P>

일부 직업인의 건강증진생활양식에 영향을 미치는 요인 연구

이은경,안병상,유택수,김성천,정재열,박용신,장두섭,송용선,이기남,Lee Eun-Kyoung,An Byung-Sang,Yu Taek-Su,Kim Seoung-Cheon,Jeung Jea-Yeal,Park Young-Shin,Jahng Doo-Sub,Song Yung-Sun,Lee Ki-Nam 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.2

The current industrial health service is shifting to health improvement business with 1st primary prevention-focused service from secondary and tertiary prevention-focused business, and Oriental medicine can provide such primary prevention-focused service due to the characteristics of its science. In particular, the advanced concept of health improvement can match the science of health care of Oriental medicine. Notably, what is most important in health improvement is our lifestyle, This does not underestimate the socio-environmental factors, which have lessened their importance due to modernism. The approach of Oriental medicine weighs more individuals' lifestyle and health care through self-cultivation. This matches the new model of advanced health business. Oriental medicine is less systemized than Western medicine, but it can provide ample contents that enhance health. If we conceive health-improvement program based on the advantages provided by these two medical systems, this will influence workers to the benefit of their health. Also, health Program needs to define factors that determine individual lives, and to provide information and technologies essential to our lives. The Oriental medicine approach puts more stress on a subject's capabilities than it does on the effect his surrounding environment can have. This needs to be supported theoretically by not only defining the relations between an individual's health state and his lifestyle, but also identifying the degree to which an individual in the industrial work place practices health improvement lifestyle . This is the first step toward initiating health-improvement business . In order to do this, this researcher conducted a survey by taking random samplings from workers, and can draw the following conclusions from it. 1 The sampled group is categorized into', by sender, female 6.6%, and male 93.4%, with males dominant; by marriage status , unmarried 43.9% and married 55.6%, with both similar percentage, and, by age, below 30, 48.4%, between 30 and 39, 27.4%, between 40 and 49, 18.2%, and over 50, 6.0%. The group further is categorized into; by education, middle school or under 1.7%, high school 30.5%, and junior college or higher 65.8% with high school and higher dominant: and by income, below 1.7 million won 24.2%, below 2.4 million won 14.8%, and above 2.4 million 6.3% Still, the group by job is categorized into collegians with 23.9%, office worker with 10.3%, and professionals with 65.8% , and this group does not include workers engaged in production that are needed for this research, but mostly office workers . 2. The subjects selected for this survey show their degree of practicing health-improvement lifestyle at an average of 2.63, health management pattern at 2.64, and health-related awareness at 2.62 The sub-divisions of health-improvement lifestyle show social emotion (2.87), food (2.66). favorite food (2.59), and leisure activities (2.52), in this order for higher points. It further shows health awareness (2.47) and safety awareness (2.40), lower points than those in health management pattern . 3. In the area of using leisure time for health-improvement, males, older people, married, and people with higher income earn higher marks. And, in the area of food management, the older and married earn higher marks . In the area of favorite food management, females, lower-income bracket, and lower-educated show higher degree of practice , while in the area of social emotion management, the older. married, and higher-income bracket show higher marks. In addition, in the area of health awareness, the older, married, and people with higher-income show higher degree of practice. 4. To look at correlation by overall and divisional health-improvement practice degree , this researcher has analyzed the data using Person's correlation coefficient. The lifestyle shows significant correlation with its six sub-divisions, and use of leisure time, food, and health awareness al