가토에서 경정맥 이식을 이용한 요도결손 재건술

정재호,이상원,설정현,김효헌 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.5

There are several methods for the reconstruction of partial urethral defect, including split-thickness skin graft, full-thickness skin graft, and preputial flap and free flap. Despite partial success using these methods, most results are unsatisfactory due to frequent complications such as postoperative infection, fistula formation, hair growth and stricture. There have been several pioneering experiments in this field using autogenous vein graft and the results have been controversial. However, the fact that the endothelial lining is replaced by natural urethral epithlium is generally accepted as a positive effect of this method. This experiment was designed to identify the possibility of using vein graft for the reconstruction of partial urethral defect. Two different types of vein graft method were performed in a total of 20 New Zealand White rabbits with partial urethral defect. Firth, for the patch-graft group, a 1×1 cm rectangular vein graft was sutured at a defective area of the same size. Second, for the tubed-graft group, a vein graft segment 1 cm long was replaced at a segmental urethral defect of the same length. Histologic study was performed at three and eight weeks postoperatively in each group. The process of transitional ell epithelial replacement within the grafted vein was uniformly observed in both experimental groups at three weeks postoperatively. At eight weeks postoperatively, the epithelial replacement was almost complete and histologically undistinguishable. In the retrograde urethrogram performed after 8 weeks, the urinary flow in the patch-graft group was normal and showed no stricture, and two of five rabbits in the tubed-graft group showed partial stricture at the graft site. In conclusion, natural urethral epithelium was restored in grafted venous segments irrespective of the type of graft. Partial stricture was observed in 40% of the tubed-graft group while complete reconstruction was possible in the patch-graft group.

장방형내 저온 단일 수평 원관 주위의 동결현상에 관한 실험적 연구

정현철,윤재원,장원택,전승배,이동석,김병철 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.1

An experimental study on water in a rectangular cavity with a horizontal cooling tube was carried out to investigate the flow character and thermal behavior in the system. Each experiment was performed with variation of initial temperature and cooling tube temperature. When the cooling tube temperature was -4℃, the supercooling phenomenon was observed regardless of the initial water temperature. And the case of cooling tube temperature was -6℃ with initial temperature was below 4℃, the supercooling phenomenon was also observedin the large region of the test section. When the cooling tube temperature was -10℃, the local region of supercooling phenomenon was observed during the freezing process. And the higher initial temperature was, the higher supercooling degree and the less difference of the ice thickness between upward and downward was observed. It is shown that by controllingthe supercooling phenomena and the cooling tube temperature, it would lead to an increase efficiency of ice making.

인터리버를 이용한 eHDR-WPAN 시스템의 성능 분석

정승희,이현재,오창헌 한국기술교육대학교 2006 論文集 Vol.12 No.1

In this paper, we propose a performance of improvement method for eHDR-WPAN system with Interleaver. The pattern of burst error caused by fading in indoor wireless channel. For the reason, using of Interleaver(make burst error to random error) can be enhanced to error-rate in eHDR-WPAN system. Interleaver at the simulation considered Convolutional, Block and Random, respectively. Available symbol spacing of Interleaver is assumed 9 and 27. In results, Block-Interleaver is shown that a performance of improvement about 0.6dB of E_(b)/N_(o) at 10^(-4). Therefore, the suitable Interleaver for eHDR-WPAN system was 9 symbol spacing of Block Interleaver.

Penicillium oxalicum Var.가 生産하는 Glucoamylase에 關한 硏究

鄭宰顯 忠州大學校 1987 한국교통대학교 논문집 Vol.20 No.2

These experiments were conducted to investigate the purification and the properties of glucoamylase produced by Penicilliun oxalicum Var. The results obtained were as follows. 1. Two forms of glucoamylase from Penicillium oxalicum were purified by (NH₄)₂SO₄fractionation, acetone fractionation and succesive column chromatography on DEAE-cellulose, and they were designated glucoamylase Ⅰ(GⅠ) and glucoamylase Ⅱ(GⅡ), respectively. 2. The specific activities of glucoamylase Ⅰ and glucoamylase Ⅱ toward soluble starch were 178 u/mg protein, and 51.5 u/mg protein, and the yields of them were 178 u/mg protein, and 51.5u/mg protein, and the yields of them were 5.8% and 1.8%, respectively. 3. The two purified enzyme were proved to show a single band by polyacrylamide disc gel electrophoresis, and molecular weights of glucoamylase Ⅰ and glucoamylase Ⅱ were estimated to be approximated 84,000 and 86,000 by through Sephadex G-100 Gel filtration, respectively. 4. The optimum pH of glucoamlase Ⅰ and glucoamylase Ⅱ were pH 5.0 and 4.5 respectively. The stable pH ranges of them were pH 3.0-6.5, and optimum temperature of them were 55-60℃ and 60℃, respectively. The stable temperature of them were below 55℃, respectively. 5. Both of the glucoamylase hydrolyzed amylose amylopectin, glycogen and soluble starch, but did not act on isomaltotriose, isomaltose, cellebiose, lactoss, turanose and dextran. 6. Both of the glucoamylase were inhibited by the addition Hg??, Pb??, and the hydrolysis product toward soluble starch of the enzymes were proved to be only glucose by paper chromatography.

Bacillus polymyxa가 生産하는 β- amylase에 關한 硏究

鄭宰顯 忠州大學校 1988 한국교통대학교 논문집 Vol.22 No.-

These experiments were conducted to investigate the purification and the properties of β-amylase producted by Bicallus polymyxa. The results obtained were as follows : 1. The optimum culture time, initial pH and culture temperature were 100hrs., pH7.0 and 30℃, respectively. 2. The crude enzyme was purified by starch absorption, ammonium sulfate fractionation, DEAE-cellulose column chromatography, CM-cellulose column chromatography and Bio Gel p-150 column chromatography. The specific activity of the purified enzyme was 143u/mg. protein and the yield of enzyme activity was 10.1% 3. The purified enzyme showed a single band on polyacrylamide gel electroporesis. Molecular weight of the purified enzyme was estimated to be approximately On the production conditions of α-amylase from Bacillus subtilis MY-1012. 4. The optimum pH, temperature, the stable pH and temperature range of the purified enzyme were pH 7.0∼8.0, 40℃, pH4.0∼9.0 and below 55℃, respectively.

유청배지에서 Propionibacterum Feudenreichii와 Lactobacillus Brebis와의 혼합배양에 의한 프로피온산의 생산

鄭宰顯 충주대학교 1997 한국교통대학교 논문집 Vol.32 No.2

Pure cultures of Propionibacterium freudenreichil did not grow in autoclave-sterilized cheese whey(121℃,15 psi, 20min)at whey concentrations greater than 2% (w/v) spray-dried sweet dairy whey. Propionic acid was produced from autoclave-sterilized whey by growing Propionibacterium freudenreichii in mixed culture with Lactovacillus breves. In medium containing 5-12% autoclaved whey solids and 1% yeast extract, the mixed culture pocuced 1.3-3.0% propoinic acid, 0.5-1.0% acetic acid, and 0.05-0.80% lactic acid.All the lactose was consumed. With pH-controlled fementors (pH=7.0),mixed cultures produced at least 30% more popionic acid than cultures in which pH was not controlled.

Bovine botulism outbreak associated with incidental consumption of presumably contaminated leftover food

( Jae Won Byun ),( Kyung Hyun Lee ),( Ha Young Kim ),( Soon Seek Yoon ),( Ji Youl Jung1 ),( Woo Seog Jeong1 ),( Jae Ku Oem ),( Jong Soo Lim ),( Myoung Heon Lee ),( You Chan Bae ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1

Nine cattle among 18 in a native Korean herd died or were euthanized within 3 days. The affected cattle showed cowshed. The pigs and dogs had been fed leftover food originating from nearby restaurants, and the cattle could presumably reach the contaminated food in the pig troughs. Necropsy revealed a few chicken bones and red pepper pieces as well as large amounts of grain and rice straw hay mixed in the rumen. Botulism type D was isolated in the ruminal contents of one of the cattle. We speculated that the outbreak was associated with the cattle incidentally eating presumably contaminated leftover food from the pig trough.

혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

교사들의 댄스스포츠 참여가 여가인지와 결혼만족에 미치는 영향

정애월,김재현 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

Consequently it obtains the following conclusions. First, as a result of analyzing their recognition of leisure end satisfaction with marriage life according to demographical characteristics, there was a significant difference in self-confidence in leisure among four factors of recognition of leisure by workplace and in affectionate relationship of couple among four factors of satisfaction with marriage life. There was a significant difference in desire for leisure and self-confidence in leisure among four factors of recognition of leisure by age and in two factors including affectionate marital relationship and authoritative marital relationship among four factors of satisfaction with marriage life by age. Second, as a result of analyzing relationship between participation in dance sports, recognition of leisure and satisfaction with marriage life, there was a significant difference in desire for leisure and self-confidence in leisure among four sub-variables of recognition of leisure and the recognition of leisure in general was higher in participants than in non-participants and it can be assumed that these results have affirmative influence of participation in dance sports on recognition of leisure. Satisfaction with marriage Life showed a significant difference in affectionate marital relationship, adaptation to marriage life and authoritative marital relationship among four sub-variables of satisfaction with marriage life according to participation or non-participation in dance sports, Third, as a result of verifying the relationship between degree of participation in dance sports and satisfaction with marriage life, influence of intensity of participation in dance sports (β=.246) has a significant influence on the recognition of leisure and satisfaction with marriage life has a significant correlation with frequency of participation in dance sports and frequency of participation (β=.176) has a significant influence on satisfaction with marriage life.

Bacillus Circulans IAM 1112로부터 Maltotetraose와 Maltopentaose를 생산하는 Amylase의 특성

鄭宰顯 忠州大學校 1994 한국교통대학교 논문집 Vol.29 No.2

The maltooligosaccharide producing amylase was extracted from culture broth of Bacillus circulans IAM 1112 and was puried by ammonium sulfate fractionation and DEAE-cellulose column chromatography. The puried anzyme was homogenous on polyacrylamide gel disc electrop-horesis. The optimum temperature and pH of reaction enzyme were 50℃ and 6.0, respectively pH stability range and themal stability of puried enzyme were pH 5.0-10.0 and stable below 55℃. The enzyme was perfectly inhibited by metal ions such as Hg??,Ag?? and not inhibited Ca??.