식이 Selenium 함량이 납중독 흰쥐 간조직의 항산화계와 세포 손상에 미치는 영향

임정교,이순재 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.4

This study was to investigate the effect of selenium on hepatic antioxidative defense system and oxidative damage in lead-administered rats. Male Sprague-Dawley rats weighing 140±5g were divided into one normal group(Se, 0 ppm) and three lead groups according to dietary levels of selenium supplementation: Pb0(Se, 0 ppm), PbS(Se, 0.5 ppm), and PbSS(Se, 1.0 ppm). All experimental groups were fed the experimental diet ad libitum for 4 weeks, and lead groups fed one containing 2,000 ppm lead acetate. Liver superoxide dismutase(SOD) activities in Pb0 group increased compared with other experimental groups. Liver gluthathione peroxidase(GSH-px) activities in Pb0 group decreased compared with normal group, but those of PbS and PbSS groups significantly increased compared with Pb0 group. Glutathione S-transferase(GST) activities decreased in Pb0 group and not significantly different from PbS and PbSS groups compared with normal group. Reduced glutathione(GSH) contents and GSH/GSSG of liver in Pb0 group were lower than those of other groups, Liver vitamin E contents in Pb0 group were about 50% of the normal group, but those of PbSS and PbS increased more than Pb0 group. Liver damage in electron microphotography process decreased in RER, showed an increase in lysosome and also an increase in swelling of mitochondria, and ordered as follows: PbSS. PbS, and Pb0. It was concluded that high levels of dietary selenium had protective effects on peroxidative damage of hepatic cell accompanied with increased antioxidative defense system in lead-administered rats.

표면 플라즈몬 공명 신호의 증폭을 위한 새로운 금 콜로이드 센서칩 제조와 특성 연구

임정혁,이경호,고광락,강신원,김재호 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

New gold colloidal film sensor chips were employed to enhance the sensitivity of the Surface Plasmon Resonce (SPR). Au collodal solutions were synthesized, and Au colloidal films were fabricated by immobilization of colloid on vacuum deposited Au film through the coupling agent of 1,8-diaminooctane. Specific interaction of human IgG antibody to human antigen was monitored by in situ SPR measurement from both a smooth and a Au colloidal films. Au colloidal film sensor chips resulted in 27 % enhancements effect for the same reaction on SPR angle shifts compared to a smooth Au film.

Sprague Dawley 수컷 랏트에서 Bisphenol A Diglycidyl Ether의 경구 투여에 의한 급성 독성

임재언,양윤정,이태진,홍연표 대한산업의학회 2006 대한직업환경의학회지 Vol.18 No.4

목적: 본 연구는 BADGE의 경구 투여에 의한 급성 독성학적 연구를 수행하여 기존의 연구 결과를 확인하고 추후 내분비계 장애 연구에 필요한 기초자료를 얻고자 하였다. 방법: 수컷 SD 랏트에 0, 1000, 2000, 4000과 8000 mg/kg/day의 BADGE와 DES 0.37 mg/kg/day의 농도를 단회 경구로 투여한 후 14일간 일반 증상 등을 관찰하였고 14일 후 부검하였다. 결과: BADGE를 투여한 모든 군에서 투여 후 3일째에 설사와 다른 일반 증상들이 관찰되었고 체중도 감소하는 경향을 보였다. 특히 8000 mg/kg/day의BADGE를 투여한 랏트에서 투여 후 3일째에 대조군에 비해 유의한 수준으로 체중이 감소하는 것을 관찰할 수 있었다. 일부 BADGE 투여군에서 대조군에 비해 심장(1000, 2000과 4000 mg/kg/day), 간(1000, 2000, 4000과 8000 mg/kg/day)과 전립샘(4000 mg/kg/day)의 무게의 감소가 관찰되었다. BADGE 투여군의 간(1000과 4000 mg/kg/day)과 전립샘(8000 mg/kg/day) 의 상대 무게가 대조군과 차이를 보였다. 고환을 제외한 모든 장기에서 조직학적인 변화는 관찰되지 않았으며 고환에서는 세 정관에서 정자세포의 감소가 관찰되었다. 고환과 부고환의 sperm head 수를 보면, 고환에서만 감소하는 경향을 보였으며 정자의 운동성과 기형은 대조군과 차이가 없었다. 혈장 에스트로겐과 테스토스테론 농도는 대조군과 모든 투여군 간의 유의한 차이가 없었다. 결론: BADGE를 랏트에 경구 투여 하였을 때 1000 mg/kg/day의 수준에서도 일반 독성 및 생식독성을 유발시킬 수 있음을 보여준다. Objectives: Bisphenol A Di Glycidyl Ether (BADGE) is the major component in commercial liquid epoxy resins, which are manufactured by co-reacting bisphenol A with epichlorohydrin. The authors investigated the acute toxicity of BADGE. Methods: BADGE was administered by a gavage to 8 week old SPF Sprague Dawley rats in a single dose of 0 (negative control), 0.37 (Diethylstilbesterol, DES), 1000, 2000, 4000, and 8000 mg/kg/day of BADGE. Each treatment group contained 7 rats. The general status and weight of the rats were observed for 14 days. The rats were anesthetized by ether at 14 days, and the changes in morphology, organ weight, sperm count and motility, and hormone level were measured. Results: All the rats treated with BADGE had diarrhea on the 1st day. The rats administered BADGE at 1000, 2000, and 4000 mg/kg/day showed a soiled perineal region and soft stools with diarrhea until the 3rd day. The 8000 mg/kg/day BADGE rats had diarrhea for two days followed by emaciation, soiled fur, a soiled perineal region, staining around the mouth and were moribund for three to eight days. No weight gain was observed after the 1st day in the 2000, 4000, and 8000 mg/kg/day BADGE rats and after the 7th day in all the treatment groups compared with the control groups. Some treatment groups were observed to have a decrease in the weight of the heart (BADGE 1000, 2000 and 4000 mg/kg/day), liver (BADGE 1000, 2000, 4000 and 8000 mg/kg/day) and prostate (BADGE 4000 mg/kg/day) compared with control group. The weight of the liver was significantly lower in all treatment groups compared with the control group. The relative weight of the liver (BADGE 1000 and 4000 mg/kg/day) was significant lower than the control. No pathological changes were observed in the brain, liver, thyroid, heart, spleen, kidney, lung and prostate. The number of spermatid in the seminiferous tubule in the testes was lower in all treatment groups than the control. The sperm motility tended to decrease with increasing concentration but the sperm count was similar in all treatment groups. The plasma Estrogen and testosterone level were similar in the control and treatment groups. Conclusions: These results suggest that BADGE induces general, hepatic and reproductive toxicity at 1000 mg/kg/day.

임상실습시 간호학생의 자기노출과 불안과의 관계

정재임,차영자 최신의학사 1993 最新醫學 Vol.36 No.3

운동제어의 신경생리학적 기초

정성태,홍승길,임재형 師範大學 體育硏究所 1994 서울大學校 體育硏究所論集 Vol.15 No.2

Many motor activities of animals and human beings are a critical aspect for their survival. Although it has not been clearly understood how motor activities are produced, the idea that various motor programs exist somewhere in the central nervous system (CNS) is generally accepted. As evidence for existence of motor programs in the CNS, we may consider a simple, repetitive movement like walking. Walking does not require continuous thinking. Once it is initiated, it becomes quite automatic. If we try to control consciously our automated movement, then it becomes so unnatural. Edward Evart, a neurophysiologist, noticed in his monkey experiments that the arm muscle contraction for handle movement was preceded by the activation of neurons in the motor cortex that governs the arm movement. These experimental results may be taken as evidence for existence of central programs for movements in the motor cortex. Many other studies, however, have indicated that cerebellum is an important structure where motor program are stored. Potentially, motor programs may not be restricted to a particular site in the CNS. Instead, they may be distributed in the cortex. cerebellum and other neural structures such as the basal ganglia. To understand movements at the neural level, we should determine how movements are coded in the CNS. More specifically, we may have to reveal how neurons are interconnected and how they communicate with each other to ultimately produce movements. An important thing to realize before we attempt to answer these questions is the fact that there is no clear-cut boundary between voluntary and involuntary movements. Even in the voluntary activities such as tennis game, there are lots of involuntary components. Also, in many other cases, there is good evidence that perfect movements can be achieved involuntarily. Diving, which requires a rapid, coordinated control of eyes, arms, legs and other parts of the body, is a good example. To perform this type of movement, there must be a motor program. Cerebellum has been indicated as a key structure necessary for this kind of program. Cerebellum is known as a site involved in the control of independent limb movements, especially rapid, skilled movements. Such movements are initiated by neurons in the frontal association cortex, which control neurons in the primary motor cortex. Both the frontal association cortex and the primary motor cortex send information about intended movements to the cerebellum. Information from the somatosensory system, which informs about the current position and rate of the movement of the limbs-information necessary for computing the details of a movement-is also sent to the cerebellum. When the cerebellum receives the information that the motor cortex has begun to initiate a movement, it computes the contribution that various muscles will have to make to perform that movement. The result of this computation is sent back to the motor cortex. Thus, the information from the cortex enables the cerebellum to modify the ongoing movement initiated by the frontal cortex. The capacity of the cerebellum to fine-tune motor drive generated in the motor cortex may be enhanced by training. In this case, the training will influence the involuntary components of movement.

세포브러쉬와 면봉도말법에 의한 부인과 세포학적 검사의 효율비교

김정란,김성숙,배한익,김용탁,심재철,임문환,윤혜원,이상식 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

A study was undertaken to evaluate the efficiency of the sytobrush compared with the cotton swab for obtaining cells from the uterine cervix. In 300 patients, the cytobrush technique was employed and in control 300 patients was smeared by the cotton swab. In 12 out of 300 patients(4%) endocervical cells were found on the cotton swab technique. A total of 252 out of 300 cytobrush sampler specimen(84%) contained endocervical cells. With the X²method for statistical comparison, the difference in endocervical cell yield between the cotton swab and the cytobrush sampler was significantly different(p<0.01). This was particularly true in older patients. We conclude from study that the cytobrush sampler is of value for obtaining as adequate cervical cytologic specimen.

개에 발생한 만성 승모판폐쇄부전증에 대한 Pimobendan 치료 1례

장재임,전형규,최호정,이영원,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2006 動物醫科學硏究誌 Vol.14 No.-

A 11-year-old intact male maltese dog with respiratory distress, cyanosis and coughing was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Loud holosystolic murmur with a palpable precordial thrill was heard at the left apex region by auscultation. Left sided cardiac enlargement and mitral regurgitated flow were detected by thoracic radiography and echocardiography, respectively. Clinical signs were improved by treatment of diuretics, ACE inhibitor and pimobendan.

비호지킨림프종 환자에서 발생한 폐 효모균증 1예

장재혁,송치원,심병용,이동건,변재호,정정임,이교영,홍영선,신완식,김춘추,이경식 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

저자들은 비호지킨씨 림프종 환자에서 항암 치료 후 질환의 진행성 병변과 감별을 요했던 우폐 중엽의 단발성 종괴를 흉강경하 생검으로 폐 효모균증을 확진하고 amphotericin B와 fluconazole로 치료한 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Non-Hodgkin's lymphoma is monoclonal expansion of malignant B or T cells. The immunocompromised status in this disease is accompanied by many infections. The cryptococcosis, caused by Cryptococcus neoformans, frequently occurs in leukemia, Hodgkin's disease, sarcoidosis, diabetes mellitus, tuberculosis, and long-term steroid-using patients. Recent increasing incidence of fungal infection could be due to the spread of AIDS and transplantation. We experienced one patient with lung mass in Non-Hodgkin's lymphoma after three cycles of chemotherapy, which could not be discriminated from the newly developed lymphoma mass. Cryptococcus neoformans was isolated from the lung tissue obtained by thoracoscopic biopsy. Herein we report this case with brief review of pertinent literature.

Delay Line을 이용한 1차 미분 임펄스 발생기 설계

김은정,임재현,김길겸,김학선 한밭대학교 정보통신전문대학원 2004 정보통신전문대학원 논문집 Vol.2 No.1

본 논문에서는 UWB (Ultra-WideBand)시스템에 사용되는 짧은 폭의 임펄스 발생기를 설계하였다. SRD와 두개의 단락된 전송 선로를 이용 하였다. 단락된 전송 선로는 시간 지연과 위상의 반전을 효과적으로 제공 한다. RC 미분기 대신에 단락된 전송 선로(Delay Line)을 이용하여 1차 미분 임펄스를 발생시켰다. 시뮬레이션을 통해 700psec의 펄스폭과 600mV(p.p)의 진폭, 펄스 모양의 좋은 대칭성을 확인하였고 또한, 펄스 주변의 떨림 현상이 거의 발생하지 않았다. In this paper, we have designed impulse generator of short pulse width for UWB(Ultra-Wide band) system using SRD and two short-circuited stubs. The short-circuited stub provides good time delay and reverse of phase. The first derivative impulse was generated by using short-circuited stub(Delay Line) instead of RC differentiator. A first derivative impulse of 700 psec pulse duration, 600mV(p.p) pulse amplitued, and good symmetry has been demonstrated though simulation. Also, there are extremely small amount of ringing around the first derivative impulse.

각종 소화성질환에서의 Campylobacter Pylori의 검출에 관하여

허정욱,강영우,박준호,박승국,하경임,김재룡,안성훈,박선우 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

Peptic disorders are among the most common maladies in korea as the world. In most cases their etiologies not be established and their pathophysioolgy remain obscure. Although the persence of fastric bacteria has been logn established, recently campylobacter pylori seems to be etiologic agent of peptic disorders. Campylobacter pylori is visualized in most cases of peptic disorders and not visualized after treatment of bctericidal agents, bismuth compound or metronidazole. We had a following results for identification of campylocacter pylori in stomach and duodenal mcuosa via endoscipc biopsy. 1. We studied 54 patients who had dyspeptic symptoms, 29 males. 2. Most patients are in 5th decades, next in 3rd and 6th in order. 3. Ptients were composed of 27 peptic ulcer, 22 gastitis and 5 gastric carcinoma. 4. Campylobacter pylori was identified in 69% of total patients, peptic ulcer 63%, gastritis 77%, stomach carcinoma leastly.