Hypoxia Mediates Runt-Related Transcription Factor 2 Expression via Induction of Vascular Endothelial Growth Factor in Periodontal Ligament Stem Cells

Manjing Deng,Qian Xu,Zhihua Liu1,Ling Guo,Rui Liu,Rulei Li,Xiang Chu,Jiajia Yang,Jia Luo,Faming Chen 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11

Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible factor-1α (HIF-1α) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of HIF-1α, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of HIF-1α, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride (CoCl2, 100 μmol/L), an agonist of HIF-1α, and suppressed by 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10 μmol/L), an antagonist of HIF-1α. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF (hVEGF165) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via HIF-1α-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs

p38 MAPK Signaling Mediates Mitochondrial Apoptosis in Cancer Cells Induced by Oleanolic Acid

Liu, Jia,Wu, Ning,Ma, Lei-Na,Zhong, Jia-Teng,Liu, Ge,Zheng, Lan-Hong,Lin, Xiu-Kun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Oleanolic acid (OA) is a nutritional component widely distributed in various vegetables. Although it has been well recognized for decades that OA exerts certain anti-tumor activity by inducing mitochondria-dependent apoptosis, it is still unclear that what molecular signaling is responsible for this effect. In this study, we employed cancer cell lines, A549, BXPC-3, PANC-1 and U2OS to elucidate the molecular mechanisms underlying OA anti-tumor activity. We found that activation of MAPK pathways, including p-38 MAPK, JNK and ERK, was triggered by OA in both a dose and time-dependent fashion in all the tested cancer cells. Activation was accompanied by cleavage of caspases and PARP as well as cytochrome C release. SB203580 (p38 MAPK inhibitor), but not SP600125 (JNK inhibitor) and U0126 (ERK inhibitor), rescued the pro-apoptotic effect of OA on A549 and BXPC-3 cells. OA induced p38 MAPK activation promoted mitochondrial translocation of Bax and Bim, and inhibited Bcl-2 function by enhancing their phosphorylation. OA can induce reactive oxygen species (ROS)-dependent ASK1 activation, and this event was indispensable for p38 MAPK-dependent apoptosis in cancer cells. In vivo, p38 MAPK knockdown A549 tumors proved resistant to the growth-inhibitory effect of OA. Collectively, we elucidated that activation of ROS/ASK1/p38 MAPK pathways is responsible for the apoptosis stimulated by OA in cancer cells. Our finding can contribute to a better understanding of molecular mechanisms underlying the antitumor activity of nutritional components.

국제상사중재상 택일조항과 기판력원칙의 적용에 관한 고찰

류쟈쟈(Jia-Jia Liu),한낙현(Nak-Hyun Han) 한국국제상학회 2023 國際商學 Vol.38 No.1

본 연구에서는 국제상사중재에서 자주 쟁점이 되고 있는 기판력조항과 택일조항을 분석하고, 이들 조항과 관련하여 쟁점이 된 Iberdrola v. Guatemala(Ⅱ) 사건을 분석한 후 그 시사점을 제시하고 있다. 이 사건에서 ① 기판력(res judicata)의 문제는 국제법과 중재지법(스위스법)에 근거하여 판정되었다. ② 3중 동일성 기준(triple identity test)에 근거해 전 ICSID 중재의 관할권 부정 판정의 기판력을 인정하고, UNCITRAL 중재에서 신청인의 청구가 차단되었다. ③ 투자조약상에는 명시적인 택일(fork in the road) 조항이 존재하지 않았지만, 해석에 의해 택일조항의 존재를 인정한 후에 그것을 적용하여 관할권을 부정했다. ④ 반소에 대해 투자조약과 중재규칙이 모순되는 경우에 있어서는 전자가 우선한다고 해, 반소를 할 권리를 피신청국은 갖지 않는다고 판정했다는 점에서 의의가 있다고 본다. Purpose: By analyzing the res judicata principle and the fork-in-the-road clause, which are frequently at issue in international commercial arbitration, this study presents the implications related to these clauses after analysis of Iberdrola v. Guatemala (II). Research design, data, and methodology: This study mainly adopts the literature research method, and utilizes domestic and foreign literature as well as Internet data. Results: ① The issue of res judicata was decided based on international law and the law of the place of arbitration (Swiss law). ② Based on the triple identity test, the ICSID recognized the arbitration’s denial of jurisdiction under the res judicata principle, and the UNCITRAL claimant was blocked in arbitration. Conclusions: It is meaningful that it was decided that the respondent country does not have the right to file a counterclaim, saying that the former takes precedence in cases where the investment treaty and the arbitration rules contradict each other.

Depressive symptoms and cognitive impairment in patients with systematic lupus erythematosus: The mediating role of sleep quality and the moderating role of complement

Xiaotong Liu,Yane Lu,Xinlei Jia,Ya Bin Wei,Xiao Wang,Jia Jia Liu 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

Mental health conditions in patients with systemic lupus erythematosus: A systematic review and Meta-analysis

Xiaotong Liu,Xiao Wang,Xinlei Jia,Ya Bin Wei,Jia Jia Liu 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

Bio-inspired synthesis and characterization of mesoporous ZnFe2O4 hollow fibers with enhancement of adsorption capacity for acid dye

Jia Liu,Dickon H.L. Ng,Peng Song,Yi Song,Chao Kong 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.23 No.-

Magnetic ZnFe2O4 hollow fibers were synthesized via a facile bio-template method involving porefabricating and nanoparticle assembling process. The as-prepared kapok-morphic ZnFe2O4 exhibited hollow fiber structure composed of nanocrystallites with grain sizes of 30–50 nm. The adsorption kinetics equilibrium and thermodynamics of acid fuchsin onto ZnFe2O4 were investigated. It was found that the adsorption was spontaneous and exothermic, and the adsorption process was well described by both the Langmuir isotherm model and the pseudo-second order kinetics model. The maximum adsorption capacity of ZnFe2O4 hollow fibers for acid fuchsin was 150.37 mg/g. Electrostatic absorption was conceived as the main adsorption mechanisms.

Lignin Based Flexible Electromagnetic Shielding PU Synergized with Graphite

Jia Zhang,Yunxia Qi,Yumei Zhang,Jinchi Duan,Bairun Liu,Baijun Liu,Zhaoyan Sun,Yiquan Xu,Wei Hu,Niaona Zhang 한국섬유공학회 2021 Fibers and polymers Vol.22 No.1

In this paper, lignin (L)-based polyurethane (FeGLPU) with excellent electromagnetic shielding properties wasprepared and studied. The modified reduced iron powder was modified with KH550, and then the obtained modified reducediron powder (Fe) was mixed with lignin, polyethylene glycol 200 (PEG200), hexamethylene diisocyanate (HDI) and graphite(G) to in-situ synthesize the FeGLPU. The thermal stability, mechanical properties, electronic conductivity, and morphologyof the composite FeGLPU were characterized in detail. The electromagnetic interference shielding effectiveness (EMI SE) ofFeGLPU was tested in the frequency range of 8.2-26.5 GHz. When the content of Fe and G was fixed at 10 % and the lignincontent was 5 %, the maximum EMI SE was 21.6 dB, and the frequency width of EMI SE greater than 10 dB was 18.3 GHz. The conductivity was 4.27×10-4 S/m, and the tensile strength of Fe10G10L5PU reached 11.7 MPa. When the lignin contentincreased to be 20 %, the maximum EMI SE was 22.5 dB, and the frequency width of EMI SE greater than 10 dB was18.3 GHz. The conductivity was 1.06×10-2 S/m, and the thermal decomposition temperature T5 (5 % weight loss) reached234 °C. The obtained excellent EMI SE should be due to the synergistic effect of Fe, G and lignin. It can be observed fromthe SEM observation that the G and Fe are uniformly dispersed in PU matrix with strong interfacial interaction. It wasillustrated that the prepared FeGLPU was with efficient electromagnetic shielding properties, good mechanical properties,and high thermal stability. This lignin based electromagnetic shielding PU was proposed to have broad application prospectsdue to its low expenses and ecology friendliness.

Overlapping Stents-Assisted Coiling for Vertebral Artery Dissecting Aneurysm : LVIS Stent within Neuroform EZ Stent

Liu, Xing-Long,Wang, Bin,Zhao, Lin-Bo,Jia, Zhen-Yu,Shi, Hai-Bin,Liu, Sheng The Korean Neurosurgical Society 2022 Journal of Korean neurosurgical society Vol.65 No.4

Objective : To evaluate the safety and efficacy of an overlapped stenting-assisted coiling technique in treating vertebral artery dissecting aneurysm (VADA) via Low-profile Visualized Intraluminal Support (LVIS) stent-within-Neuroform EZ stent. Methods : From January 2017 to June 2019, 18 consecutive patients with VADAs (ruptured : unruptured=5 : 13) were treated with the overlapping stents assisted-coiling technique in our center. The overlapping manner was a Neuroform EZ stent being deployed first, followed by LVIS stents placement using the 'shelf' technique. The patients' clinical characteristics, technical feasibility and safety, and immediate and follow-up angiographic results were retrospectively reviewed. Results : Seventeen (94.4%) procedures were technically successful with an exact deployment of the stents and patent parent or perforator arteries. The immediate angiographies after procedure confirmed Raymond class I, II, and III occlusion of VADAs were in 12 (66.7%), two (11.1%), and four cases (22.2%), respectively. Post-procedural complications developed in one patient (5.6%) with minor brainstem infarctions, which resulted from an in-stent thrombosis during the procedure. Angiographic follow-up at 5.7 months (range 3 to 9 months) demonstrated Raymond class I and II occlusion were in all cases (100%). The modified Rankin Scale scores at 21.3 months (range 15 to 42 months) 0-2 in 17 cases (94.4%) and three in one case (5.6%). Conclusion : Overlapping stents via LVIS stent-within-Neuroform EZ stent combined with coiling is safe and effective for patients with VADA in the midterm results.

Fresh Washed Microbiota Transplantation Alters Gut Microbiota Metabolites to Ameliorate Sleeping Disorder Symptom of Autistic Children

Liu Nai-Hua,Liu Hong-Qian,Zheng Jia-Yi,Zhu Meng-Lu,Wu Li-Hao,Pan Hua-Feng,He Xing-Xiang 한국미생물학회 2023 The journal of microbiology Vol.61 No.8

Accumulating studies have raised concerns about gut dysbiosis associating autism spectrum disorder (ASD) and its related symptoms. However, the effect of gut microbiota modification on the Chinese ASD population and its underlying mechanism were still elusive. Herein, we enrolled 24 ASD children to perform the first course of fresh washed microbiota transplantation (WMT), 18 patients decided to participate the second course, 13 of which stayed to participate the third course, and there were 8 patients at the fourth course. Then we evaluated the effects of fresh WMT on these patients and their related symptoms. Our results found that the sleeping disorder symptom was positively interrelated to ASD, fresh WMT significantly alleviated ASD and its sleeping disorder and constipation symptoms. In addition, WMT stably and continuously downregulated Bacteroides/ Flavonifractor/Parasutterella while upregulated Prevotella_9 to decrease toxic metabolic production and improve detoxification by regulating glycolysis/myo-inositol/D-glucuronide/D-glucarate degradation, L-1,2-propanediol degradation, fatty acid β-oxidation. Thus, our results suggested that fresh WMT moderated gut microbiome to improve the behavioral and sleeping disorder symptoms of ASD via decrease toxic metabolic production and improve detoxification. Which thus provides a promising gut ecological strategy for ASD children and its related symptoms treatments.

A Parallel Method of Deep Packet Inspection based on Message-Passing Interface

Jia-xing Qu,Guo-yin Zhang,Xi-zhong Wang,Jia-hui Liu,Da-hua Song 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12

With the increasing number of cores in multicore processors, it is challenging task how to take advantage of powerful parallel computing for the deep packet inspection. This paper introduces the deep packet inspection with a parallel method which exploits the message-passing interface (MPI). The parallel procedure includes the master thread and the slave thread. The master assigns the data packet to the slave. The slave executes the string matching with rules for inspecting. Both the master and the slave communicate by using MPI functions. The experimental results show that the parallel method is suitable for the trend of the increasing number of cores in multicore processors. Moreover, when the number of threads is equal to the number of cores in multicore processors, the performance arrives at the maximum throughput.