http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics
Martha Schamel,Jake E. Barralet,Jürgen Groll,Uwe Gbureck 한국생체재료학회 2017 생체재료학회지 Vol.21 No.2
Background: In vitro cell testing of degradable bioceramics such as brushite or monetite is often challenging due to the ion release into or adsorption from the culture medium. These ionic changes are then mostly responsible for cell proliferation and activity, which prohibits the investigation of effects originating from surface topography or further material modifications. Methods: Here, we aimed to solve this problem by developing a pre-conditioning regime following the repeated immersion of brushite and monetite samples in various Ca2+, Mg2+ and PO4 3− containing electrolytes, followed by studying ion adsorption / release as well as changes in phase composition and in vitro cytocompatibility with MG63 cells. Results: The results demonstrated that by using DMEM cell culture medium in a ratio of 10 ml/sample was sufficient to minimize changes of ionic composition after 7 d with a daily change of the medium. This leads to changes of the surface composition with dissolution of the brushite phase. In turn, this also positively influences the in vitro cytocompatibility with a 2–3 fold higher cell number and cell activity on the DMEM pretreated surfaces. Conclusions: Controlled sample washing prior to cell testing using DMEM medium seems to be a valuable procedure not only to stabilize the pH during cell culture but also to maintain ion concentrations within a cell friendly range.
Oliver Berberich,Julia Blöhbaum,Stefanie Hölscher-Doht,Rainer H. Meffert,Jörg Teßmar,Torsten Blunk,Jürgen Groll 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-
Tissue adhesives play an important role in clinical applications and may aid in the treatment of cartilagedefects for improved cartilage integration. However, many fail to satisfy the demand for adequateadhesive strength on wet tissue surfaces and to facilitate sufficient cell migration and extracellular matrix(ECM) deposition at the defect site. Utilizing poly(2-alkyl-2-oxazoline) (POx)-based polymers equippedwith mussel-inspired adhesion moieties and combining them with the natural wound sealantfibrinogen,we fabricated an adhesive biosynthethic hydrogel with tunable mechanical properties and improvedbonding strength. Degradation of the hydrogels could be adjusted by the ratio of amide to ester linkagesof the catecholic functional group at the POx side chain. In an in vitro disc/ring model for lateral cartilageintegration, a benefit in long-term integration was observed with enhanced degradation of the adhesivewithout the expense of bonding strength. Incorporation of degradable ester linkages in the polymerfacilitated cell invasion and strong deposition of cartilaginous ECM at the defect site. Overall, the resultssuggest that the presented injectable adhesive hydrogel, due to its easy tunability, holds great potentialfor cartilage defect treatment and other medical applications.
Biofabrication: reappraising the definition of an evolving field
Groll, Jü,rgen,Boland, Thomas,Blunk, Torsten,Burdick, Jason A,Cho, Dong-Woo,Dalton, Paul D,Derby, Brian,Forgacs, Gabor,Li, Qing,Mironov, Vladimir A,Moroni, Lorenzo,Nakamura, Makoto,Shu, Wenmiao,Ta IOP Publishing 2016 Biofabrication Vol.8 No.1