Tannic acid-induced apoptosis in FaDu hypopharyngeal squamous cell carcinoma

Loan Thi Ta,Trang Thi Kieu Nguyen,Hoon Yoo 대한구강생물학회 2019 International Journal of Oral Biology Vol.44 No.2

Tannic acid (TA) is a water-soluble polyphenol compound found in various herbal plants. We investigated the chemopreventive effects of TA on FaDu hypopharyngeal squamous carcinoma cells. In an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, TA showed dose-dependent cytotoxicity with a half maximal inhibitory concentration (IC50) of 50 µM. Cell cycle analysis and immunofluorescence imaging demonstrated that under low-dose (25 µM) treatment, FaDu cells were arrested in G2/M phase, and as the dose of TA was increased, apoptosis was induced with the increase of cell population at sub-G1 phase. The expressions of various cyclins, including cyclin D1 and cyclin-dependent kinases (CDK-1 and CDK-2), were down-regulated at low doses of TA, whereas apoptotic effectors such as cleaved caspase 3, cleaved caspase 7, and poly (ADP-ribose) polymerase (PARP) were expressed in a dose-dependent manner in Western blotting. In addition, TA-induced apoptosis of FaDu cells might be mediated by the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase pathway, with the upregulation of p-AKT/p-PKB (phosphorylated protein kinase B) and p-ERK. Overall, our data support the hypothesis that TA is a potential candidate agent for the treatment of hypopharyngeal cancer.

Investigation of the Relationship Between Cognitive Functions and Retinal Findings From Spectral Optical Coherence Tomography in Patients With Schizophrenia and Their Healthy Siblings

Rümeysa Taşdelen(Rümeysa Taşdelen ),Batuhan Ayık(Batuhan Ayık ),Hatice Kaya(Hatice Kaya ),Neslihan Sevimli(Neslihan Sevimli ) 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.3

Objective Retinal structural changes which were investigated by optical coherence tomography (OCT) have been reported in schizophrenia. Since cognitive dysfunction is a core feature of schizophrenia, the correlations between retinal findings and cognitive functions of patients and their healthy siblings may provide insight into the pathophysiological processes of the disorder. We aimed to investigate the relationship between neuropsychiatric tests and retinal changes in schizophrenia patients and their healthy siblings.Methods We measured OCT parameters and cognitive performance (via Trail Making Tests, verbal fluency tests, and The Digit Span Tests) of 72 participants (36 patients with schizophrenia and 36 healthy siblings) and disease severity (with Positive and Negative Syndrome Scale, Global Assessment of Functioning, and Clinical Global Impression scales) in patients with schizophrenia and evaluated the relationship between retinal findings and clinical parameters, especially neurocognitive tests.Results We found decreased ganglion cell layer-inner plexiform layer thickness and macular volume in the patient group. There were strong correlations between neurocognitive tests and OCT findings in both groups. On the other hand, there was not any correlation between retinal findings and disease parameters.Conclusion The cognitive symptoms of schizophrenia may be more closely related to structural changes in the retina.

Physciosporin suppresses the proliferation, motility and tumourigenesis of colorectal cancer cells

Taş,, İ,sa,Han, Jin,Park, So-Yeon,Yang, Yi,Zhou, Rui,Gamage, Chathurika D.B.,Van Nguyen, Tru,Lee, Ji-Yoon,Choi, Yong Jae,Yu, Young Hyun,Moon, Kyung-Sub,Kim, Kyung Keun,Ha, Hyung-Ho,Kim, Sang Elsevier 2019 Phytomedicine Vol.56 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Lichens, which represent symbiotic associations of fungi and algae, are potential sources of numerous natural products. Physciosporin (PHY) is a potent secondary metabolite found in lichens and was recently reported to inhibit the motility of lung cancer cells via novel mechanisms.</P> <P><B>Purpose</B></P> <P>The present study investigated the anticancer potential of PHY on colorectal cancer (CRC) cells.</P> <P><B>Methods</B></P> <P>PHY was isolated from lichen extract by preparative TLC. The effect of PHY on cell viability, motility and tumourigenicity was elucidated by MTT assay, hoechst staining, flow cytometric analysis, transwell invasion and migration assay, soft agar colony formation assay, Western blotting, qRT-PCR and PCR array <I>in vitro</I> as well as tumorigenicity study <I>in vivo</I>.</P> <P><B>Results</B></P> <P>PHY decreased the viability of various CRC cell lines (Caco2, CT26, DLD1, HCT116 and SW620). Moreover, PHY elicited cytotoxic effects by inducing apoptosis at toxic concentrations. At non-toxic concentrations, PHY dose-dependently suppressed the invasion, migration and colony formation of CRC cells. PHY inhibited the motility of CRC cells by suppressing epithelial-mesenchymal transition and downregulating actin-based motility markers. In addition, PHY downregulated β-catenin and its downstream target genes cyclin-D1 and c-Myc. Moreover, PHY modulated KAI1 C-terminal-interacting tetraspanin and KAI1 expression, and downregulated the downstream transcription factors c-jun and c-fos. Finally, PHY administration showed considerable bioavailability and effectively decreased the growth of CRC xenografts in mice without causing toxicity.</P> <P><B>Conclusion</B></P> <P>PHY suppresses the growth and motility of CRC cells via novel mechanisms.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Treatment of hydrocyanic galvanic effluent by electrocoagulation: Optimization of operating parameters using statistical techniques and a coupled polarity inverter

Taís Sabedot Pertile,Eliena Jonko Birriel 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.10

The treatment of hydrocyanic galvanic effluent with Zn, Ni and Cu ions by electrocoagulation (EC) using aluminum electrodes and a polarity inverter was evaluated. 32 experiments were performed and the optimal operating conditions were obtained using statistical analysis: 30minutes, 5.00 g/L NaCl, 1 cm between electrodes, electrode surface area of 104cm2/L and current density of 8mA/cm2. The results for removal with the polarity inverter were: 99.55% total cyanide, 22.49% total nickel, 52.66% total copper and 100% total zinc. After the EC experiments, localized and general corrosion of the aluminum electrodes was evaluated both internally and externally by SEM. Contaminant removal was confirmed by X-Ray powder diffraction (XRD) and Fourier transform infrared (FT-IR) which demonstrated the presence of contaminants in the galvanic sludge generated in the process.

Corrosion and Wear Characteristics of Electroless Ni–P, Ni–P–W and Composite Ni–P–W/Al2O3 Coatings on AZ91 Sheet

Selim Taşci,Reşat Can Özden,Mustafa Anik 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.2

Electroless Ni–P–W was coated on AZ91 sheets with various W contents and both the corrosion and wear resistance characteristicsof these coatings were observed. The increase in W content resulted in a decrease in P content and an increase inthe crystallinity of the electroless coatings. The corrosion resistance of the electroless coatings increased upon increase inW content of the electroless coatings up to approximately 5%. Above this content, however, the corrosion resistance tendedto decrease due to the disturbed grain structure of the electroless coating. But both the surface hardness and wear resistanceincreased concurrently due to the positive contribution of W solid solution hardening. The applied heat treatment resulted ina decrease in the corrosion resistance due to the disappearance of the amorphous structure and increase in both the surfacehardness and wear resistance due to the precipitation hardening. Al2O3dispersion was better with the nonionic surfactant,as compared to the anionic and cationic surfactants. Composite coating reduced the corrosion resistance and increased thewear resistance of the electroless Ni–P–W coatings. The applied heat treatment was observed to have positive contributionto Ni–P–W coatings in getting the optimum corrosion and wear resistance combination.

Severe Facial Dermatitis Following Rhinoplasty due to an Unusual Etiopathogenesis: Rosacea

Süleyman Taş 대한성형외과학회 2015 Archives of Plastic Surgery Vol.42 No.3

Microdissected Prefabricated Flap: An Evolution in Flap Prefabrication

Süleyman Taş 대한성형외과학회 2016 Archives of Plastic Surgery Vol.43 No.6

When traditional flap techniques are not feasible, we apply flap prefabrication, which is more complicated and sophisticated but supplies large and thin flaps. There are some disadvantages to the technique that require improvement, such as venous congestion after flap transfer, which requires months for neoangiogenesis and necessitates a vascular carrier. Here, the author presents a new technique, called as ‘microdissected prefabricated flap,’ to successfully produce a safe, large, and thin flap. This technique is based on the microdissection of the perforators to the greatest extent possible, spreading them out into the subdermal level and using them as a carrier. The details and the application of this technique are presented and reported.

Learner-friendly textbooks: Chemistry texts based on a constructivist view of learning

Uğur Taşdelen 서울대학교 교육연구소 2008 Asia Pacific Education Review Vol.9 No.2

In this study, the use of inquiry methods, learning cycles, a conceptual change model and analogy in creating alternative science texts was discussed. An alternative text on the topic of acids and bases was created by integrating the methods and models discussed in this paper. The alternative text and a sample of a traditional text taken from a textbook, which is still used in Turkish high schools, were given to two groups, totaling 42 pre-service teachers-the alternative text was given to an experimental group and the traditional text to a control group-in an experimental setting and their understandings of acids and bases were compared. In addition, in the second step of the study, the pre-service teachers read both texts and indicated their preferences in terms of interest, understandability and helpfullness.

Microshear bond strength according to dentin cleansing methods before recementation

Simge Taşar,Mutahhar Muhammed Ulusoy,Gökçe Merıç 대한치과보철학회 2014 The Journal of Advanced Prosthodontics Vol.6 No.2

PURPOSE. The aim of this study was to determine the efficiency of Erbium, Chromium: Yttrium-Scandium-Gallium-Garnet laser in different output powers for removing permanent resin cement residues and therefore its influence on microshear bond strength compared to other cleaning methods. MATERIALS AND METHODS. 90 extracted human molars were sectioned in 1 mm thickness. Resin cement was applied to surface of sliced teeth. After the removal of initial cement, 6 test groups were prepared by various dentin surface treatment methods asfollows: no treatment (Group 1), ethylene diamine tetra acetic acid application (Group 2), Endosolv R application (Group 3), 1.25 W Erbium, Chromium:Yttrium-Scandium-Gallium-Garnet laser irradiation (Group 4), 2 W Erbium, Chromium:Yttrium-Scandium-Gallium-Garnet laser irradiation (Group 5) and 3.5 W Erbium, Chromium:Yttrium-Scandium-Gallium-Garnet laser irradiation (Group 6). The topography and morphology of the treated dentin surfaces were investigated by scanning electron microscopy (n=2 for each group). Following therepetitive cementation, microshear bond strength between dentin and cement (n=26 in per group) were measured with universal testing machine and the data were analyzed by Kruskal Wallis H Test with Bonferroni correction (α<.05). Fracture patterns were investigated by light microscope. RESULTS. Mean microshear bond strength ± SD (MPa) for each group was 34.9 ± 17.7, 32.1 ± 15.8, 37.8 ± 19.3, 31.3 ± 12.7, 44.4 ± 13.6, 40.2 ± 13.2 respectively. Group 5 showed significantly difference from Group 1, Group 2 and Group 4. Also, Group 6was found statistically different from Group 4. CONCLUSION. 2 W and 3.5 W Erbium, Chromium: Yttrium-Scandium-Gallium-Garnet laser application were found efficient in removing resin residues.

An Unexpected Complication after Periorbital Tumor Surgery in a Patient Later Discovered to Have Myelodysplasia

Süleyman Taş,Hüsamettin Top 대한성형외과학회 2014 Archives of Plastic Surgery Vol.41 No.2