애플망고 미성숙과 추출물의 생리활성에 대한 연구

유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회 2021 대한미용학회지 Vol.17 No.4

In the current study, we investigated the biological activities of ethanol extract of unripe Mangifera indica L. var. Irwin using a variety of in vitro and cell culture model systems for investigation of the anti-inflammatory and whitening effects. A total polyphenol, electron donating, 2, 2’-azinobis [3-ethylbenzothiazoline-6- sulfonic acid] (ABTS) radical scavenging assay was used for measurement of the antioxidant activities of unripe Mangifera indica L. var. Irwin. The content of phenolic compounds in unripe Mangifera indica L. var. Irwin was 121.47±0.47 mg/100 g. In measurement of electron donating and ABTS radical scavenging ability of unripe Mangifera indica L. var. Irwin, the effect was shown in a concentration-dependent manner. Inhibition of tyrosinase activity was 40.69% at 1,000 μg/ mL concentration of unripe Mangifera indica L. var. Irwin. An MTT assay was used to evaluate cell viability in order to evaluate the anti-inflammatory and whitening effects of unripe Mangifera indica L. var. Irwin in macrophages (RAW 264.7 cells) and melanoma (B16F10 cells). The result showed more than 95% at a concentration of 500 μg/ mL in RAW 264.7 cells and more than 95% at a concentration of 100 μg/mL in B16F10 cells. Nitric oxide inhibitory activity was confirmed for measurement of anti-inflammatory activity. Consequentially, unripe Mangifera indica L. var. Irwin showed an inhibitory effect of 62.64% at a concentration of 500 μg/mL. The amount of melanin production was measured in order to confirm the whitening effect; the inhibitory effect was 15.16% at a concentration of 100 μg/ mL. Thus, unripe Mangifera indica L. var. Irwin could be applied as an anti-inflammatory and whitening effect and could be used in the future as a safe, natural material for use in cosmetics.

십이지장 궤양 발생에 있어서 H . pylori와 위상피화생의 연관에 대한 연구

박선미(Seon Mee Park),양석균(Suk Kyun Yang),홍원선(Weon Seon Hong),민영일(Young Il Min),이인철(In Chul Lee) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.6

N/A Background/Aims: H. pylori infection and gastric metaplasia are frequently associated in patients with duodenal ulcers. However, the pathogenesis of duwlenal ulcer in relation to H, pilori infecri<in and gastric metaplasia has not reacbed a consistent result, yet. The aim of this study v,as to assess whether H. pylori infection and gastric metapla.ia contrihute to duodenal ulcerogenesis. Methods: Multiple endoscopic biopsy specimens were tnken frorn duodenum and gastric antrum of ?I subjects(21 active duodenal ulcers; 30 scarred duodenal ulcers; 20 normal controls). The specimens were examined histologically for the presence ot' H. pylori, gastric metaplasia, and degree of gastritis and duodenitis. Rapid tissue urease test was performed t'or detection of H, pi krri infc cti<in. Results: The positive rate of H. pylrri in gastric antrurn was not significantly different aniong ttlTCC groups, while it was increasing in duodenum, I,e., normal controls(3%'), scarred DU's(37,r), active DU's(62%) in ascending order. The incidence of duodenal gastric metaplasia in the active DU'.I was 95.2% in contrast with the scarred duodenal ulcer's(72.07<) and the nonnal contrnls(2i.04) Diffuse gastric metaplasia lesions were more prevalent in active duodenal ulcers. 1n the duodenum, a strong association was shown between the H. pylrri and gastric rnetaplasia. The severity ot' duodenitis were significantly increased in the order ot' groups of normal controls, scarrecl duodena1 ulcers, and active duodenal ulcers. In duoclenum, there was a strong association with the grade c>f' intlammation anci H. pylori. Conclusions: These results suggest that gaslric metaplasia in the duodenum and concurrent H. pilori infection may play a synergistic role in the pathogene.I. Vt' duodenal ulcer. (Korean J Gastroenterol 199S;27: 617-62S)

Gel-Gas-Gel 삼중 충돌에 의한 젤 모사 추진제의 분열 특성

이인철 ( In Chul Lee ),김상선 ( Sang Sun Kim ),구자예 ( Ja Ye Koo ) 한국액체미립화학회 2009 한국액체미립화학회 학술강연회 논문집 Vol.2009 No.-

春秋決獄의 槪念에 대한 再檢討

李仁哲(Lee In-chul) 동양사학회 1997 東洋史學硏究 Vol.57 No.-

에탄올 혼합비에 따른 3중 충돌형 인젝터의 분무특성

이인철 ( In Chul Lee ),김종현 ( Jong Hyun Kim ),구자예 ( Ja Ye Koo ) 한국항공운항학회 2007 한국항공운항학회지 Vol.15 No.4

Spray characteristics of a unlike triplet injector were investigated experimentally. Spray images and SMD of droplet were measured to evaluate the spray characteristics injected by liquid/gas combinations. G-L-G(Gas-Liquid-Gas), L-G-L(Liquid-Gas-Liquid) type of injector were used by changing the gas and liquid feed lines. The SMD graph shows that the droplet sizes decrease in the out of radial direction at a fixed gas Reynolds number. The SMD value of decreasing tendency shows that the more ethyl alcohol ratio increase, the more SMD value decrease.

젤 모사 추진제 삼중 충돌 분사 제트의 거시적 분열 특성 연구

이인철 ( In Chul Lee ),구자예 ( Ja Ye Koo ),황태진 ( Tae Jin Kwang ) 한국분무공학회 2010 한국액체미립화학회지 Vol.15 No.3

원산지별 그린커피빈에 대한 항산화 및 항염증 비교 연구

유단희(Dan-Hee Yoo),이인철(In-Chul Lee) 한국인체미용예술학회 2020 한국인체미용예술학회지 Vol.21 No.3

In this study, the extracts of green Coffea arabica bean by country of origin were tested as a potential source for natural ingredients in cosmetic products. Antioxidative activities were measured by total polyphenol, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and 2,2 -azino-bis (3- ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Anti-inflammatory activities were checked by nitric oxide (NO) assay. The total polyphenol content was found to be higher in ethanol extract than in water extracts, and in particular Ethiopia ethanol extracts had 852.39 mg/100 g, making them the highest in content. The antioxidant measurement was measured with DPPH and ABTS, indicating that antioxidant strength increased dependent on the concentration of all extracts to be superior. The cell survival rate of the extracts of green Coffea arabica beans by country of origin was confirmed by MTT assay and was close to 100 ㎍/㎖ to 100%. Anti-inflammatory activity by NO assay showed lipopolysacchride (LPS)-induced NO was significantly inhibited following treatment with extract of green Coffea arabica beans by country of origin and decreased in a concentration-dependent manner. This study confirmed the efficacy of green Coffea arabica beans by country of origin through research related to antioxidants and anti-inflammatory, and confirmed its availability as a natural material for cosmetics.

애플망고 가지 추출물의 항산화 활성 및 대식세포내의 iNOS, COX-2의 염증인자 발현 저해 효과

유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회(구 대한미용과학회) 2020 대한미용학회지 Vol.16 No.4

In the present study, anti-oxidant and anti-inflammation activities of distilled water extract (AMBW) and 70% ethanol extract (AMBE) of Mangifera indica L. var. irwin branches were investigated. The total polyphenol content of M. indica L. var. irwin branch extracts was 523.24±1.03 (AMBW), 556.15±2.80 (AMBE) mg TAE/100 g dry weight. The anti-oxidant activity of the extracts was estimated using electron donation and scavenging activity of 2,2'-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals which increased with concentration. To measure the anti-inflammation effects, RAW 264.7 cells treated with lipopolysaccharide were used. The cell viability of M. indica L. var. irwin branches extracts in RAW 264.7 cells was measured with 3-[4,5-dimethyl-thiazol-2-yl]-2,5- diphenyl-tetrazoliumbromide (MTT) assay. The results of MTT assay showed more than 95% refers to cell viability at the 100 μg/mL of AMBW and AMBE. The production of nitric oxide (NO) analyzed by griess assay decreased with increasing concentration of the extracts. The mRNA expression toward inducible NO synthase and cyclooxygenase-2 was confirmed using reverse transcription-polymerase chain reaction and inhibited the treated M. indica L. var. irwin branches extracts. In conclusion, M. indica L. var. irwin branch extracts were confirmed to have antioxidant and anti-inflammation properties and can be used as a natural ingredient of cosmetic materials.

한약재 복합 추출물의 항산화, 항균 및 항염 효과

이인철 ( In Chul Lee ),김미경 ( Mee Kyung Kim ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.5

Objectives: This paper aimed to verify the applicability of mixed extract of Angelica gigas Nakai, Cnidium officinale Makino, Paeoniala ctiflora Pall, Rechmannia glutinosa Libosch, Scutellaria baicalensis Georgi, which were prescribed for improving inflammation in Donguibogam, as the materials for beauty food and functional medicinal herb cosmetics by manufacturing such mixed extract and evaluating the biological activity of the extract. Methods: The mixed medicinal herb water extract(MMW) and ethanol extract(MME) were freeze-dried to be used as the specimen. We performed electron donating ability, lipid acidification inhibitory activity, anti-inflammatory activity against skin flora, MTT assay, NO inhibitory activity and the protein expression inhibitory activity of iNOS and COX-2. Results: For anti-oxidation experimentation, the electron donating abilities of MMW and MME were above 60.0% and 90.0% at 500 μg/ml, respectively. In the inhibition rate of lipid peroxidation, MMW and MME showed 43.1% and 52.1% at 1,000 μg/ml, respectively. As a result of antimicrobial activity, both the MMW and MME showed significant clear zones for Propionibacterium acnes at 4 mg/disc, but did not indicated the clearzones for Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis. Anti-inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited in a concentration-dependent manner. Also, the expression of iNOS and COX-2 proteins were significantly inhibited following treatment with MMW and MME of 50 μg/ml. Conclusions: Accordingly, it can be concluded that mixed medicinal herb extract has the potential to beused as a functional food and cosmetic material.

온청음 추출물을 첨가한 화장품 안정성 연구

이인철(In-Chul Lee),김미경(Mee-Kyung Kim) 한국인체미용예술학회 2015 한국인체미용예술학회지 Vol.16 No.4

The stability of Onchungeum ethanol extract was investigated to determine its applicability as a functional cosmetic material. For its industrial application, the cosmetics were prepared with a water/oil/water(W/O/W) multiple emulsion. The results of the stability test showed that the W/O/W multiple emulsion containing ethanol extract of Onchungeum was highly stable for 60 days without change in particle stability, pH, viscosity, temperature and exposure to artificial lighting. These results suggest that an ethanol extract of Onchungeum can be a useful functional cosmetics material for improving skin diseases.