참깨 추출물의 항산화 활성 및 melanoma cell (B16F10)에서 MITF, TRP-1, TRP-2, tyrosinase의 발현 저해

유단희(Dan-Hee Yoo,),주다혜(Da-Hye Joo),이진영(Jin-Young Lee) 한국생명과학회 2017 생명과학회지 Vol.27 No.3

본 연구는 참깨 70% 에탄올 추출물의 항산화 효과와 미백효과를 검증하여 나타내었다. 참깨 추출물의 전자공여능 측정실험은 1,000 μg/ml에서 71.7%의 효과를 나타냈으며, tyrosinase 저해활성 측정은 1,000 μg/ml의 농도에서 42%의 효과를 보였다. 참깨 추출물의 세포 생존율을 melanoma cell (B16F10)에서 확인하기 위하여 MTTassay를 진행하였으며, 세포 생존율을 측정한 결과, 1,000 μg/ml 농도에서 84.3%의 독성을 보였다. 이하의 세포실험에서는 세포 생존율이 90% 이상되는 농도인 500 μg/ml 이하에서 실험을 진행하였다. 참깨 추출물의 MITF, TRP-1, TRP-2 및 tyrosinase의 단백질 발현 효과를 50, 250, 500 μg/ml 농도에서 측정하였으며, 그 결과 MITF, TRP-1, TRP-2 및 tyrosinase의 각각 500 μg/ml 농도에서 68.3%, 39.2%, 89.7%, 22.3%의 단백질 발현 억제 효과를 보였다. 또한, MITF, TRP-1, TRP-2 및 tyrosinase의 mRNA발현을 RT-PCR로 50, 250, 500 μg/ml 농도에서 측정하였고, 양성 대조군으로 GAPDH를 사용하였다. 그 결과, 참깨 추출물의 500 μg/ml 농도에서 각각 81.8%, 66.5%, 84.2%, 68.1%의 mRNA 발현이 감소함을 확인할 수 있었다. 이상의 결과로 부터 참깨 추출물의 화장품 소재로서의 가능성을 확인할 수 있었다. This study was performed to improve the antioxidant and skin-whitening activities of 70% ethanol extract from Sesamum indicum L. (SIL). The electron-donating ability of the SIL extract was 71.7% at a concentration of 1,000 μg/ml. The whitening effects that was measured by tyrosinase inhibition assay. As a result, SIL extract was shown 42% at 1,000 μg/ml concentration. The cell toxicity on B16F10 melanoma cells of SIL of 70% ethanol extract showed 84.3% at 1,000 μg/ml concentration. The microphthalmia-associated transcription factor (MITF), tyrosinase relate protein-1 (TRP-1), tyrosinase relate protein-2 (TRP-2) and Tyrosinase protein and mRNA expression inhibitory effect of SIL extract were measured by western blot and reverse transcription- polymerase chain reaction (PCR) at 50, 250, 500 μg/ml concentration. Consequently, the MITF, TRP-1, TRP-2, Tyrosinase protein expression inhibitory effect of SIL extract was decreased by 68.3%, 39.2%, 89.7%, 22.3%, respectively, at 500 μg/ml concentration. Moreover, MITF, TRP-1, TRP-2, Tyrosinase mRNA expression inhibitory effect by reverse- transcription-PCR of SIL extract was decreased by 81.8%, 66.5%, 84.2%, 68.1%, respectively, at 500 μg/ml concentration. Therefore, we excellently identified the antioxidant activities and whitening effect of SIL extract, and this finding suggested that SIL extract has great potential as a cosmetic ingredients.

애플망고 가지 추출물의 항산화 활성 및 대식세포내의 iNOS, COX-2의 염증인자 발현 저해 효과

유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회(구 대한미용과학회) 2020 대한미용학회지 Vol.16 No.4

In the present study, anti-oxidant and anti-inflammation activities of distilled water extract (AMBW) and 70% ethanol extract (AMBE) of Mangifera indica L. var. irwin branches were investigated. The total polyphenol content of M. indica L. var. irwin branch extracts was 523.24±1.03 (AMBW), 556.15±2.80 (AMBE) mg TAE/100 g dry weight. The anti-oxidant activity of the extracts was estimated using electron donation and scavenging activity of 2,2'-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals which increased with concentration. To measure the anti-inflammation effects, RAW 264.7 cells treated with lipopolysaccharide were used. The cell viability of M. indica L. var. irwin branches extracts in RAW 264.7 cells was measured with 3-[4,5-dimethyl-thiazol-2-yl]-2,5- diphenyl-tetrazoliumbromide (MTT) assay. The results of MTT assay showed more than 95% refers to cell viability at the 100 μg/mL of AMBW and AMBE. The production of nitric oxide (NO) analyzed by griess assay decreased with increasing concentration of the extracts. The mRNA expression toward inducible NO synthase and cyclooxygenase-2 was confirmed using reverse transcription-polymerase chain reaction and inhibited the treated M. indica L. var. irwin branches extracts. In conclusion, M. indica L. var. irwin branch extracts were confirmed to have antioxidant and anti-inflammation properties and can be used as a natural ingredient of cosmetic materials.

B16F10 세포에서의 오크라 추출물의 미백 활성 검증

유단희 ( Dan-hee Yoo ),이인철 ( In-chul Lee ) 한국미생물 · 생명공학회 2021 한국미생물·생명공학회지 Vol.49 No.4

본 연구는 오크라 추출물의 미백 효과를 검증하여 화장품 소재로서 활용가능성을 확인하고자 하였다. 먼저, 오크라 열수 및 70% 에탄올 추출물의 미백효과를 tyrosinase의 효소 억제 활성으로 측정한 결과, 최종 농도인 1,000 μg/ml 농도에서 22.2%, 32.8%의 저해활성 효과를 보였다. 세포 차원에서 미백효과를 측정하기 위해 오크라 열수 및 에탄올 추출물의 세포 생존율을 melanoma cell (B16F10)에서 MTT assay법을 이용하여 측정하였다. 그 결과, 오크라 열수 및 70% 에탄올 추출물에서 100 μg/ml 농도에서 95% 이상의 생존율을 보였으며, 세포 독성이 나타나지 않은 농도 이하에서 멜라닌 생합성을 확인하기 위해 실험을 진행하였으며, 농도 의존적으로 멜라닌 합성을 저해하는 것을 확인하였다. 오크라 열수 및 70% 에탄올 추출물의 단백질 발현억제 효과를 5, 10, 50, 100 μg/ml의 농도에서 western blot으로 측정하였으며, 양성대조군으로 β-actin을 사용하였다. 그 결과, 오크라 열수 추출물은 100 μg/ml 농도에서 MITF, tyrosinase, TRP-1, TRP-2 인자들은 각각 88.1%, 24.8%, 62.2%, 42.9%의 효과를 나타내었다. 오크라 70% 에탄올 추출물은 100 μg/ml 농도에서 MITF, tyrosinase, TRP-1, TRP-2 인자들은 각각 65.3%, 58.3%, 66.2%, 65.3%의 효과를 나타내었다. 결론적으로 오크라 열수 및 70% 에탄올 추출물의 미백 효과가 검증되었으며, 기능성 화장품 소재로서 활용가능성을 확인하였다. In this study, the whitening effect of Abelmoschus esculentus extract was investigated to confirm its applicability in cosmetics. To determine the whitening effect, the tyrosinase-inhibitory activity of Abelmoschus esculentus hot water extract (AEWE) and 70% ethanol extract (AEEE) was measured. At the final concentration of 1000 μg/ml, AEWE showed an inhibitory activity of 22.2% and AEEE of 32.8%. To determine the whitening effect at the cellular level, the viability of melanoma cells treated with AEWE and AEEE was evaluated using the MTT assay. At concentrations of 100 μg/ml or less, both AEWE- and AEEE-treated groups showed cell survival rates of >95%. Furthermore, in both AEWE- and AEEE-treated melanoma cells, the melanin content decreased in a concentration-dependent manner. The inhibitory effects of AEWE and AEEE used at 5, 10, 50, and 100 μg/ml on protein expression were measured by western blot, with β-actin as the positive control. At a concentration of 100 μg/ml, AEWE showed an inhibitory effect of 88.1%, 24.8%, 62.2%, and 42.9% on microphthalmia-associated transcription factors (MITF), tyrosinase, tyrosinase-related proteins (TRP)-1, and TRP-2 factors, respectively. At the same concentration, AEEE showed inhibitory effect of 65.3%, 58.3%, 66.2%, and 65.3% against MITF, tyrosinase, TRP-1, and TRP-2 factors, respectively. In conclusion, the whitening effects of AEWE and AEEE were verified, and their applicability as a natural ingredient in cosmetics was confirmed.

애플망고 잎의 추출물의 항산화 및 대식세포(RAW 264.7)에서 iNOS, COX-2 발현 저해 효과

유단희(Dan-Hee Yoo),이인철(In-Chul Lee) 한국생명과학회 2020 생명과학회지 Vol.30 No.9

본 연구는 애플망고 잎 추출물의 항산화 및 항염증에 활성을 검증하여 연구하였다. 총 폴리페놀 함량은 Folin-Denis의 방법에 의해 측정하였다. 그 결과 애플망고 잎의 열수와 70% 에탄올 추출물은 각각 440.83±1.02, 475.63±1.3 mg/100 g TAE의 함량을 나타내었다. 항산화 활성을 측정하기위해 전자공여능 측정과 2,2"-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) 라디칼 소거능 활성을 측정하였으며, 모든 추출물이 우수한 효과를 나타내었다. 대식세포내에서 애플망고 잎 추출물의 세포 생존율을 MTT assay의 방법으로 측정하였다. 세포의 독성이 나타나지 않은 농도구간에서 이하의 세포실험을 진행하였다. 항염증 활성을 효과적으로 측정하기 위해 LPS로 염증반응을 유도시키고 Griess assay를 이용해 nitric oxide를 측정하였다. 그 결과 애플망고 잎 추출물은 농도의존적으로 NO의 생산을 억제함을 나타내었다. LPS로 유도된 RAW 264.7세포에서 iNOS와 COX-2의 염증인자 생성억제를 mRNA발현 억제를 RT-PCR을 통해 확인하였다. 따라서 본 연구에서는 애플망고 잎 추출물의 항산화 및 항염증 활성의 우수한 효과를 확인하였고, 그 결과 천연소재로서의 유용성과 함께 화장품의 기능성소재로서의 활용가능성을 확인하였다. The present study investigates the antioxidant and anti-inflammatory activities of Mangifera indica L. leaf extract. The total polyphenol content was measured using the Folin-Denis method. Results showed that the M. indica L. leaf extract of water and 70% ethanol showed a content of 440.83±1.02, 475.63±1.3 mg/100 g tannic acid equivalent. To assess antioxidant activity and electron-donating ability, 2,2"-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity were measured, and all extracts were found to be highly efficacious. To assess cell viability of the extract from M. indica L. leaf on macrophage cells (RAW 264.7), a 3-[4,5-dimethyl-thiazol-2- yl]-2,5-diphenyl-tetrazoliumbromide assay was performed. The following experiments were conducted in section where cells was not shown of toxicity. In order to effectively determine anti-inflammatory activity, inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells was examined using a Griess assay. The result showed that M. indica L. leaf extract concentration-dependently inhibited NO production. M. indica L. leaf extract was measured using Western blot, reverse transcription- polymerase chain reaction (RT-PCR) that to find the production of pro-inflammatory factor on stimulated RAW 264.7 cells of LPS. According to the results of this study, the M. indica L. leaf extract showed excellent effectiveness in antioxidant and anti-inflammatory activity, thus confirming its usability as a natural material and a functional raw material for cosmetics.

아티초크 추출물의 항산화, 항염증, 미백 효능 활성에 관한 연구

유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회(구 대한미용과학회) 2021 대한미용학회지 Vol.17 No.2

The objective of this study was to investigate the biological activities of 70% ethanol extracts from Cynara scolymus L. (CSEE). The total polyphenol content of CSEE were found to be 158.5 mg tannic acid/100 g dry weight of CSEE. To confirm the antioxidant effect, electron donating ability and ABTS radical scavenging effect were measured. As a result, the extent of electron donating and ABTS radical scavenging effect on CSEE increased in a dose-dependent manner. The tyrosinase inhibitory effect of CSEE, which is related to skin-whitening, was observed to be 36.25% at a concentration of 1,000 μg/mL. Furthermore, MTT assay was performed to confirm the cell viability in RAW264.7 and B16F10 cell lines. In both RAW 264.7 cell and B16F10 cells, the cell viability was observed more than 95% at 100 μg/mL in all extracts. The anti-inflammatory effect of CSEE on lipopolysaccharide (LPS)-induced RAW264.7 cells was also investigated. The CSEE significantly suppressed NO production in a concentration-dependent manner. In order to measure the whitening effect on CSEE, the expressions of proteins related to whitening, such as microphthalmia-associated transcription factors, tyrosinase-related proteins-1 were observed using western blot. In the section treated with the extracts, it was confirmed that the protein expression decreased in a concentration-dependent manner. In conclusion, CSEE was verified to have antioxidant, anti-inflammatory and whitening effects, and it was confirmed that it is a potential natural cosmetic material.

RAW 264.7 세포에서의 단풍잎돼지풀 추출물의 항염증 활성 검증

유단희 ( Dan-hee Yoo ),이진영 ( Jin-young Lee ) 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.1

본 연구는 단풍잎돼지풀 70% 에탄올 추출물의 항염증 활성을 검증하기 위해 수행되었다. 단풍잎돼지풀 70% 에탄올 추출물의 전자공여능 측정과 ABTS+ 라디칼 소거능을 측정한 결과, 1,000 μg/ml 농도에서 각각 84.1%와 92.5%의 효과를 나타냈고, 수렴활성 측정을 한 결과 1,000 μg/ml 농도에서 94.7%의 효과를 보였다. 단풍잎돼지풀 70% 에탄올 추출물의 항염증 효과를 측정하기 위해 lipopolysaccharide (LPS)로 유도된 RAW 264.7세포를 사용하여 효과를 측정하였다. 세포에서 단풍잎돼지풀 추출물의 세포독성을 측정하기 위해 MTT assay를 수행하였다. 그 결과, 500 μg/ml 농도에서 90% 이상의 생존율을 보였다. Nitric oxide 생산을 억제하는 효과를 측정한 결과, 단풍잎돼지풀 추출물에서 농도가 증가할수록 NO 생성이 감소되는 효과를 확인하였다. 단풍잎돼지풀 추출물의 단백질 발현효과를 western blot을 통해 25, 50, 100 μg/ml 농도에서 측정하였고, 양성 대조군으로 β-actin을 사용하였다. 그 결과, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 단백질 발현효과는 100 μg/ml 농도에서 8.6%, 25.1%의 감소됨을 확인하였다. ERK1/2, p38, JNK와 Iκ-Bα의 단백질발현 효과는 인산화를 통해 확인하였고, 농도의존적으로 감소하였음을 확인 하였다. mRNA 발현 억제 효과를 RT-PCR을 통해 25, 50, 100 μg/ml의 농도에서 측정하였고, 양성 대조군으로 GAPDH를 사용하였다. 그 결과, LPS로 유도된 대식세포에서 iNOS, COX-2, interleukin (IL)-1β, IL-6, TNF-α의 mRNA 발현 억제 효과는 농도가 증가할수록 발현이 감소됨을 확인하였다. 결론적으로 단풍잎돼지풀 추출물은 염증을 억제할 수 있는 가능성이 있는 항염증 소재의 가능성을 증명하였다. This study was performed to evaluate the anti-inflammatory activities of 70% ethanol extract from Ambrosia trifida L. (AT). The electron donating ability and ABTS+ radical scavenging ability of extract from AT was shown to be 84.1% and 92.5% at 1,000 μg/ml concentration. The astringent effect of extract from AT was shown to be 94.7% at 1,000 μg/ml. The anti- inflammatory activities of extract of AT were investigated using RAW 264.7 cells induced by lipopolysaccharide (LPS). The cell toxicity effect of AT extract on RAW 264.7 performed MTT assay. As a result of the measured cell toxicity effect, 90% or more was shown with cell viability at a 500 μg/ml concentration. In nitric oxide synthesis inhibition effect, it was shown that extract from AT concentration dependent inhibited nitric oxide production. The protein expression inhibitory effect of AT extract was measured by western blot at 25, 50, and 100 μg/ml concentration and the β-actin used as a positive control. Consequently, the inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 protein expression inhibitory effect was decreased by 8.6%, 25.1% at 100 μg/ml concentration. The phosphorylation of extracellular signal-regulated kinase 1/2, p38, c-Jun NH₂-terminal kinase and Iκ-Bα protein expression inhibitory effect was a decreased dependent concentration. The mRNA expression inhibitory effect was measured by reverse transcription - polymerase chain reaction at 25, 50, and 100 μg/ml concentration and the glyceraldehyde-3-phosphate dehydrogenase used as a positive control. Consequently, the iNOS, COX-2, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α mRNA expression inhibition effect was a decreased dependent concentration in an LPS-activated macrophage. In conclusion, AT extract may have some effects on inflammatory factors as potential anti-inflammatory agents and natural substance for cosmetics.

연잎 추출물의 항산화 활성 및 멜라노마 세포(B16F10)에서 MITF, TRP-1, TRP-2, tyrosinase의 발현 저해 효과

유단희(Dan-Hee Yoo),주다혜(Da-Hye Joo),이수연(Soo-Yeon Lee),이진영(Jin-Young Lee) 한국생명과학회 2015 생명과학회지 Vol.25 No.10

본 연구는 연잎 추출물의 미백 화장품 첨가물로서 사용이 가능한지를 연구하였다. 연잎 추출물의 항산화 활성을 측정하게 위해 전자공여능 측정, xanthine oxidase 억제 효과 실험을 실시하였고, 미백활성을 알아보기 위하여 tyrosinase 저해활성을 측정하여 1,000 μg/ml의 농도에서 42.7%의 효과를 나타내었다. 또한 연잎 추출물에 대한 세포생존율을 MTT assay로 측정한 결과 1,000 μg/ml 농도에서 81.61%를 이상의 세포생존율을 확인할 수 있었다. 미백 관련 인자인 MITF, TRP-1, TRP-2 및 tyrosinase의 단백질 발현과 mRNA 발현 억제를 25, 50, 100 μg/ml 농도에서 측정하였다. MITF, TRP-1, TRP-2 및 tyrosinase의 단백질 발현은 100 μg/ml 농도에서 각각 69.6%, 27.7%, 67.3%, 67.8%의 저해 효과를 나타내었고, MITF, TRP-1, TRP-2 및 tyrosinase의 mRNA발현은 100 μg/ml 농도에서 각각 67.5%, 71.4%, 85.7%, 83.6%의 억제를 나타내었다. 이러한 연구결과를 보았을 때, 연잎 추출물이 항산화 및 미백활성에 효과를 나타내었고, 화장품 첨가물로서의 가능성을 확인할 수 있었다. The purpose of this study was to investigate the potential of Nelumbo nucifera G. leaf (NNL) extract as a cosmetic additive. The electron-donating ability of the NNL extract at a concentration of 1,000 μg/ml was 67.83%. In xanthine oxidase, the inhibition effect of the NNL extract was 92.7% at the same concentration. For whitening effects, tyrosinase inhibition effect of NNL extract was 42.7% at a 1,000 μg/ml concentration. The cell toxicity of the NNL extract was examined in melanoma cells (B16F10) using a 3-[4, 5–dimethyl–thiazol–2–yl]-2, 5-diphenyl-tetrazoliumbromide (MTT) assay. The cell toxicity assay revealed that the NNL extract had a toxicity of 81.61% at a concentration of 1,000 μg/ml The microphthalmia-associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), and tyrosinase protein expression inhibitory effect by Western blot of NNL extract were measured by a Western blot at concentrations of 25, 50, and 100 μg/ml. At a 100 μg/ml concentration of the NNL extract, the expression of the MITF, TRP-1, TRP-2, and tyrosinase protein was decreased by 69.59%, 27.74%, 67.33%, and 67.78% respectively. The MITF, TRP-1, TRP-2 and tyrosinase mRNA expression inhibitory effect were measured by reverse transcription- polymerase chain reaction (PCR) at concentrations of 25, 50, and 100 μg/ml. GAPDH was used as a positive control. At a concentration of 100 μg/ml of the NNL extract, the expression of MITF, TRP-1, TRP-2, and tyrosinase mRNA was decreased by 67.51%, 71.36%, 85.74%, and 83.64%, respectively. These findings suggest that the NNL extract has antioxidant and whitening effects and that it has great potential as a cosmetic ingredient.

말오줌나무 추출물의 미백활성 검증

유단희(Dan-Hee Yoo),김진태(Jin-Tae Kim),오민정(Min-Jeong Oh),염현지(Hyeon-Ji Yeom),이진영(Jin-Young Lee) 한국생명과학회 2019 생명과학회지 Vol.29 No.3

본 연구는 말오줌나무 70% 에탄올 추출물의 항산화 효과와 미백 효과를 검증하였다. 말오줌나무 추출물의 전자공여능 측정실험은 1,000 μg/ml에서 86.21%의 효과를 나타내었고, ABTS 소거능을 측정한 결과 1,000 μg/ml 농도에서 97.9%의 소거능을 보였다. Tyrosinase 저해활성 측정은 1,000 μg/ml 농도에서 37%의 효과를 보였다. 말오줌나무 추출물의 세포 생존율을 melanoma cell (B16F10)에서 확인하기 위해 MTT assay를 진행하였는데 세포 생존율을 측정한 결과, 100 μg/ml 농도에서 90% 이상의 생존율을 보였다. 말오줌나무 추출물의 MITF, TRP-1, TRP-2 및 tyrosinase의 단백질 발현 효과를 25, 50, 100 μg/ml 농도에서 측정하였으며 양성 대조군으로 β-actin을 사용하였다. 그 결과, MITF, TRP-1, TRP-2 및 tyrosinase의 각각 100 μg/ml 농도에서 34.5%, 45.6%, 58.4%, 79.6%의 단백질 발현 억제 효과를 보였다. MITF, TRP-1, TRP-2 및 tyrosinase의 mRNA발현을 RT-PCR로 25, 50, 100 μg/ml 농도에서 측정하였고, 양성 대조군으로 GAPDH를 사용하였다. 그 결과, 말오줌나무 추출물의 100 μg/ml 농도에서 각각 85.4%, 67.5%, 85.2%, 67.1%의 mRNA 발현이 감소함을 확인 할 수 있었다. 따라서, 말오줌나무 추출물이 미백 소재로서의 가능성을 확인할 수 있었다. This study evaluated the anti-oxidant and whitening effects of a 70% ethanol extract of the Sambucus sieboldiana var. pendula (Nakai) (SS). At 1,000 μg/ml concentration, the electron donating ability of this SS extract was found to be 86.21% and the ABTS+ radical scavenging ability was 97.9%. In terms of whitening activity, the tyrosinase inhibitory effect of the extract was 37%, also at 1,000 μg/ml concentration. To explore the extractefftoxicity to B16F10 melanoma cells, a 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide assay was performed. Results showed 90% or more cells remained viable at 100 μg/ml concentration. A Western blot of the SS extract was used to measure microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1 (TRP-1), tyrosinase relate protein-2 (TRP-2), and the tyrosinase protein expression inhibitory effect at 25, 50, 100 μg/ml concentrations; β-actin was used as a positive control. Consequently, the MITF, TRP-1, TRP-2, and the tyrosinase protein expression inhibitory effect were seen to decrease by 34.5%, 45.6%, 58.4%, and 79.6%, respectively, at 100 μg/ml concentration. These were also then measured by reverse transcription-polymerase chain reaction at 25, 50, 100 μg/ml concentrations with GAPDH as a positive control. As a result, the SS extract was seen to decrease MITF, TRP-1, TRP-2, and the tyrosinase protein expression inhibitory effect by 85.4%, 67.5%, 85.2%, 67.1%, respectively at the 100 μg/ml concentration. We therefore confirmed the possibility of Sambucus sieboldiana var. pendula (Nakai) extract as a whitening material.

망고스틴 꼭지를 활용한 항산화 및 항염증에 대한 기초자료 연구

유단희(Dan Hee Yoo),이인철(In Chul Lee) K교육연구학회 2023 사회과학리뷰 Vol.8 No.4

망고스틴 꼭지 70% ethanol 에탄올 추출물(GMSE)의 항산화 및 항염증에 대해 검증한 후 천연소재로서의 활용 가능성을 확인하였다. 본 연구에서는 항산화 및 항염증 관련 천연 소재를 발굴하기 위해 GMSE을 이용하여 실험을 진행하였다. 먼저, GMSE의 총 폴리페놀 함량을 측정한 결과, 508.82±0.98 mg TAE/100 g의 함량을 확인하였다. 망고스틴 꼭지 70% 에탄올 추출물의 항산화활성을 확인하기 위해 전자공여능 및 ABTS radical 소거능을 실시하였다. 전자공여능은 최종농도인 1,000 μg/mL 농도에서 87.2%의 결과를 나타내었으며, ABTS radical 측정 결과, 50 μg/mL 농도에서부터 100%에 가까운 활성을 나타내었으며, 대조군인 Vit. C와 유사한 효과를 확인하였다. 세포 실험을 통해 항염증 활성을 측정하기 위하여 먼저 대식세포인 RAW 264.7에 관한 GMSE의 세포 생존율을 확인하였다. 이 결과를 토대로 이후 염증 관련 인자인 NO 저해활성 측정은 500 μg/mL 농도 이하에서 진행하였다. 그 결과, 최종 농도인 500 μg/mL 농도에서 26.83%의 저해 효과를 확인하였다. 이를 토대로 GMSE은 항산화 및 항염증 활성에 효과가 있음을 검증하였고, 이후 천연소재를 활용하기 위한 기초자료로 활용하고자 한다. By verifying the antioxidant and anti-inflammatory properties of Garcinia mangostana stem 70% ethanol extract (GMSE), we confirmed its utility as a natural material. In this study, experiments using GMSE were conducted to discover natural substances related to antioxidant and anti-inflammatory activities. First, as a result of measuring the total polyphenol content of GMSE, the content was confirmed to be 508.82 ± 0.98 mg TAE/100 g. To confirm the antioxidant activity of GMSE, electron donating activity and ABTS radical scavenging activity were measured. The electron donation showed an 87.2% result at a final concentration of 1,000 μg/mL, and the ABTS radical measurement showed an activity close to 100% at a concentration of 50 μg/mL, and confirmed an effect similar to that of vitamin C, the control group. In order to measure anti-inflammatory activity through cell experiments, the cell viability of GMSE for RAW 264.7 cell, a macrophage, was confirmed. Based on these results, NO inhibitory activity, a factor related to inflammation, was measured below 500 μg/mL concentration. As a result, an inhibitory effect of 26.83% was confirmed at the final concentration of 500 μg/mL. Based on this, it has been verified that GMSE is effective in antioxidant and anti-inflammatory activities, and it is intended to be used as basic data for the use of natural materials afterwards.

단풍잎돼지풀 발효 추출물의 항산화 효과 및 B16F10 세포에서의 미백 활성 검증

유단희 ( Dan-hee Yoo ),오민정 ( Min-jeong Oh ),염현지 ( Hyeon-ji Yeom ),이진영 ( Jin-young Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2020 한국미생물·생명공학회지 Vol.48 No.4

The purpose of this study was to verify the antioxidant and whitening effects of fermented Ambrosia trifida L. extract (ATFE) and to verify its usefulness as a cosmetic material. The antioxidant effects were measured by assessing the electron-donating capacity and 2,2-azino-bis(3-ethyl-benthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability of these extracts. ATFE was shown to have an electron-donation capacity of 68.4% at a concentration of 1000 μg/ml. While its ABTS+ radical scavenging ability was shown to be 58.7% at the same concentration. The ATFE tyrosinase inhibitory effect, which is related to skin-whitening, was shown to be 32.35% at a concentration of 1000 μg/ml and a cell viability assay using melanoma cells showed a 14.8% reduction in cell viability at a concentration of 100 μg/ml. Surviving cells were then used in western blot analyses to evaluate the protein inhibitory effects of ATFE at 25, 50, 100 μg/ml where β-actin was used as a positive control. The whitening effects of these extracts were also evaluated by western blot and show that the expression of microphthalmia-associated transcription factors, Tyrosinase-related proteins (TRP)-1, TRP-2 and Tyrosinase were all inhibited, 51.14%, 55.4%, 38.6%, 83.77% respectively, at 100 μg/ml ATFE. The efficacy of the whitening effects was verified and the suitability of ATFE as a cosmetic material was assured.