韓牛 末梢血液 淋巴球의 在來山羊 赤血球 Rosette形成

宋憙鍾,金鍾冕,姜明大,蔡孝錫 全北大學校 附設 畜産開發硏究所 1988 畜産開發硏究報告 Vol.2 No.-

In order to enumerate the T lymphocytes in bovine peripheral blood lymphocytes(PBL) by E rosette assay, KGRBC were treated with various concentration of 2-aminoethylisothiouronium bromide hydrobromide(AET) and dextran(Dex), singte or combination. The PBL from 10 clinically normal Korean native cattle were formed as low percentage of spontaneous E rosette(6.7±2.4%) in control group, whereas KGRBC treated with 0.1M AET for 20 minutes and 8%Dex were formed as 37.3±2.7% and 45.1±2.1%, respectively. And the synergistic effects were noted no less than 66.5±5.6% when the KGRBC treated with 0.1M AET and 8% Dex subsequently. These results suggest that the KGRBC would be a useful agent for the enumeration of T tymphocytes by E rosette assay in bovine PBI.

乳房炎 乳汁에서 分離된 原因菌의 抗菌劑 感受性

崔民淳,金鍾冕,趙正坤,蔡孝錫,姜明大,宋熹鍾 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 189 strains (Streptococcus agalactiae and non-Streptococcus agalactiae 110, Staphylococcus aureus 63, and E. coli 16) isolated from bovine mastitie milk samples by "The Connecticut Mastitis Control Program" in dairy herds were examined for drug susceptibility to 8 drugs and analysed the rate of multiple resistant patterns. 1. All of the isolates were resistant to antimicrobial agents such as penicillin (PC), streptomycin (SM), kanamycin(KM), carbenicillin (CA), lincomycin (LM), gentamicin (GM), and tetracycline (TC), singly or multiply. The isolates were more susceptible to CA (84.1%),PC (78.9%), and SM (78.4%) than the the other drugs such as GM (51.8%), KM (48.7%), TC (19.6%) and LM (19.0%) in order. 2. The multiple resistant isolates (96.3%) were more than those of singly resistant isolates (3.7%) and 58 different drug resistant patterns were observed. Among 7 singly resistant isolates, CP was the commonest. While among 182 multiple resistant isolates, CP-KM-LM-TC-GM, CP-LM-TC, CP-LM-TC-GM, PC-CP-SM-LM-TC-GM, CP-KM-LM-TC, and CP-LM patterns were frequently encounted. 3. The strains of streptococcus spp. were more susceptible to CA (90.1%) and SM (82.9%) than the other drugs such as KM (43.2%), GM (38.7%), LM (13.5%), CP (10.8%) and TC (10.8%). Among them CP-KM-LM-TC-CM pattern was frequently encounted. 4. The strains of Sta. aureus were more susceptible to PC (74.6%),CA (69.8%), SM (65.1%) and GM (63.5%) than the other drugs such as KM (46.0%), CP (22.2%), TC (15.9%) and LM (9.5%). Among them CP-LM-TC pattern was commonly encounted. 5. The strains of E. coli were more susceptible to CA (100%) and KM (75.0%) tnan TC (9.5%). But the other drugs such as PC, SM, GM, CP and LM were not. Among them PC-CP-SM-LM-TC-GM pattern was commonly encounted.

전북지방 젖소의 유방염 검색

조정곤,김종면,최민순,채효석,강명대,송희종 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 444 quarters milk samples of 111 dairy cattle in Chonbuk area were examined for infection rete and causative agents of mastitis by "the laboratory procedures used in the Connecticut Mastitis Control Program (CMCP) and California mastitis Test (CMT)". The results obtained were as follows: 1. Of 444 samples, 39.2% had positive CMCP value and 24.4% positive CMT reaction. 2. One hendred and seventy four (39.2%) from 82 cattle (73.9%) were found to infected with mastitis. It was found that 8(1.8%0 of the infected quarters were clinical mastitis and all of the rest were subclinical mastitis. 3. The main causative agents were found to Staphylococcus aureus (63 quarters), Streptococcus agalactiae (56 quarters), non-Str. agalactiae streptococci (54 querters), Escherichia coli (19 quaters), the actual total number of quarters detected as mastitis would be 174, as 18 of these quarters showed multiple infection with two different organisms. 4. Having examined the number of infection quarter per head of mastitis in dairy cattle sampling, major finding were that the average number of infection quarters to total dairy cattle exemined was 1.57 quarters, and that of the infected cattle was 2.1 quarters. Infected quarters in cattle with 1, 2, 3 and 4 were noted 27(32.9%), 28(34.1%), 17(20.7%), and 10(12.3%), respectively.

한우 T 및 B 림프구 분리 동정

채효석,송희종,김종면 한국수의공중보건학회 1991 예방수의학회지 Vol.15 No.1

Diagnosis of BVD MD in breeding Holstein cow by RT-PCR

Sung-hyo Lee,Soo-chol Han,Jong-o Lee,Yea-baek Yoon,Hee-jong Song,Hyo-seok Chai 대한수의학회 1997 대한수의학회 학술대회발표집 Vol.1997 No.-

후보자 브랜드 선택의도에 있어 정당브랜드의 조절 효과 연구

박효정(Park, Hyo-Jung),채영덕(Chai, Young-Dug),김준석(Kim, Joon-Seok) 대한경영학회 2013 大韓經營學會誌 Vol.26 No.12

Picibanil이 면양적혈구(緬羊赤血球) 감작(感作)마우스의 면역반응(免疫反應)에 미치는 영향(影響)

채효석,송희종,Chai, Hyo-seok,Song, Hee-jong 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.1

This experiment was performed on mice to investigate the effects of an immunopotentiator, picibanil(PC), on the immune responses such as phagocytic activity of reticuloen-dothelial(RE) system, E rosette formation rate of splenic lmphocytes and morphological changes of lymph node tissue. Groups of mice were treated with a single(1KE/kg BW) or sequential(0.1, 0.25 and 0.5KE/kg BW for successive 3 days) intravenous injections of PC. PC treated and untreated control mice were sensitized with 50% sheep erythrocyte suspension(0.2ml/mouse) at 1, 3, 5, 7 and 10 days after PC treatment. Functional and morphological examinations were carried out 5 days after sensitization. The following results were obtained: The phagocytic activity of RE system and the weight of liver and spleen were increased significantly at 3rd, 5th and 7th day. The peripheral polymorphonuclear leukocyte and percent of lymphocyte and monocyte were slightly increased. The rates of E rosette formation of splenic lymphotytes, sequential PC treated groups were more increased at 3rd and 5th day in sequential PC treated groups than in single treated groups. Thereafter it returned gradually to the control level by the time of 10th day. Microscopically primary lymph follicles with indistinct germinal center (GC) were partially disrupted and the parafollicular areas were consisted of the pyroninophilic cells in control group. In PC treated group, the parafollicular areas were markedly proliferated and developments of secondary lymph follicles with enlarged and prominent GC were more pronounced in the sequential injected groups compared to single injected groups. These results indicate that PC affected not only parafollicular area of the T-cell area, but also GC of the B-cell area. It suggests that PC may potentiate both cell mediated immunity and humoral immunity.

Effect of Trichloroethylene on the Induction of Rat Liver Microsomal Enzymes

Park, Sang Shin,Chai, Seok,Jeong, Hyo Seok,Chang, Sung Keun,Kim, Ki Woong 생화학분자생물학회 2000 BMB Reports Vol.30 No.4

The effects of trichloroethylene (TRf) on the induction of cytochrome P-450 (CYP) and several other related enzymes in Sprague Dawley rats were investigated. Rats were treated with TRI 150. 300. 600 ㎎/㎏ body weight in corn oil intraperitoneally once a day for 2 days. The total contents of microsomal CYP and cytochrome b_5 (b_5) decreased with the increase of TRI concentration, but the activity of p-nitrophenol hydroxylase increased with the increase of TRI dosage (p$lt;0.05). Western blot analysis which utilized monoclonal antibodies against CYP2E1 also showed a significant increase in the CYP2E band density. The increase of the activity of pentoxyresolufin-O-deethylase also was observed with the TRI treatment (p$lt;0.05) although there was no significant increase in the cytochrome CYP2B1/2 in Western blotting. The TRI did not affect the induction of aryl hydrocarbon hydroxylase. These findings suggest that the CYP2E1 is the primary enzyme which could be induced by TRI treatment in rats.

Effect of Trichloroethylene on the Induction of Rat Liver Microsomal Enzymes

Chang, Sung-Keun,Jeong, Hyo-Seok,Chai, Se-Ok,Kim, Ki-Woong,Park, Sang-Shin Korean Society for Biochemistry and Molecular Biol 1997 Journal of biochemistry and molecular biology Vol.30 No.4

The effects of trichloroethylene (TRI) on the induction of cytochrome P-450 (CYP) and several other related enzymes in Sprague Dawley rats were investigated Rats were treated with TRI 150. 300. 600 mg/kg body weight in corn oil intra peritoneally once a day for 2 days. The total contents of microsomal CYP and cytochrome $b_5\;(b_5)$ decreased with the increase of TRI concentration. but the activity of p-nitrophenol hydroxylase increased with the increase of TRI dosage (p<0.05). Western blot analysis which utilized monoclonal antibodies against CYP2E1 also showed a significant increase in the CYP2E band density. The increase of the activity of pentoxyresolufin-O-deethylase also was observed with the TRI treatment (p<0.05) although there was no significant increase in the cytochrome CYP2B1/2 in Western blotting The TRI did not affect the induction of aryl hydrocarbon hydroxylase. These findings suggest that the CYP2E1 is the primary enzyme which could be induced by TRI treatment in rats.

중국산 김치 수입과 국산 채소류 가격의 인과관계 분석

이현근 ( Lee Hyun-keun ),석준호 ( Seok Joon-ho ),김상효 ( Kim Sang-hyo ),문한필 ( Moon Han-pil ),채상현 ( Chai Sang-hyen ) 한국식품유통학회 2020 食品流通硏究 Vol.37 No.1

This study investigates the casual relationship between kimchi imports from China, prices of napa cabbage, dried red pepper, garlic, and onion in Korea considering the increase trend of kimchi imports from China. To analyze the level of relative explanation power between kimchi imports and prices of raw materials for kimchi, this paper utilizes the Geweke(1982) method. Furthermore, we also adapt the Directed Acyclic Graph method to capture the contemporaneous causation. While kimchi imports granger cause napa cabbage price, napa cabbage price leads kimchi imports based on the contemporaneous causation concept. Kimchi imports also granger cause prices of dried red pepper and garlic. And this granger causations’ explanation levels on bivariate linear relationship are very high. Our results imply that kimchi imports from China have an impact on kimchi producers and consumers as well as producers for raw materials of kimchi in Korea. In turn, the government should develop the general policy to maintain stability of supply and demand for kimchi’s raw materials.