Session K : Prokaryotic Gene Structure and Expression : Autocatalytic Processing of Penicillin G Acylase in E . coli

Jin Young Seo,Hyen Sam Kang 생화학분자생물학회(구 한국생화학분자생물학회) 1993 추계학술대회집 Vol.- No.-

Cytotoxicity and DNA Topoisomerase Inhibitory Activity of Benz[f]indole-4,9-dione Analogs

PARK, Hyen Joo,LEE, Hyun-Jung,LEE, Eun-Jin,HWANG, Hye Jin,SHIN, Sang-Hee,SUH, Myung-Eun,KIM, Choonmi,KIM, Hwa Jung,SEO, Eun-Kyung,LEE, Sang Kook 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12

A series of benz[f]indole-4,9-diones, based on the antitumor activity of 1,4-naphthoqninone, were synthesized and evaluated for their cytotoxic activity in cultured human cancer cell lines A549 (lung cancer), Co12 (colon cancer), and SNU-638 (stomach cancer), and also for the inhibition of human DNA topoisomerases Ⅰ and Ⅱ activity in vitro. Several compounds including 2-amino-3-ethoxycarbonyl-N-mcthyl-benz[f]indole-4,9-dione showed a potential cytotoxic activity judged by IC_(50)< 20.0 ㎍/㎖ in the panel of cancer cell lines. Especially, 2-hydroxy-3-ethoxycarbonyl-N-(3,4-dimethyl-phenyl)-benz[f]indole-4,9-dione had potential selective cytotoxicity against lung cancer cells (IC_(50)=0.4㎍/㎖)) compared to colon (IC_(50)>20.0㎍/㎖) and stomach (IC_(50)>20.0㎍/㎖) cancer cells. To further investigate the cytotoxic mechanism, the effects of test compounds on DNA topoisomerase Ⅰ and Ⅱ activities were used. In a topoisomerase Ⅰ-mediated relaxation assay using human placenta DNA topoisomerase Ⅰ and supercoiled pHOTI plasmid DNA, 2-amino-3-ethoxycarboHyl-N-(4-fluorophenyl)-benz[f]indole-4,9-dione had the most potent inhibitory activity among the compounds tested. However, most of the compounds showed only weak inhibition of the DNA topoisomerase Ⅱ-mediated KDNA (Kinetoplast DNA) decatenation assay, except for 2-amino-3-cthoxycarbonyl-N-(4-mcthylphcnyl)-benz[f]indole-4,9-dione and 2-amino-3-ethoxycarbonyl-N-(2-bromoehtyl)-benz[f]indole-4,9-dione with a moderate inhibitory activity. These results suggest that several active compounds had relatively selective inhibitory activity against toposiomearse I compared to toposiomerase Ⅱ. No obvious correlation was observed between the cytotoxicity of the individual compound and the inhibitory activity of DNA relaxation and decatenation by topoisomerase Ⅰ and Ⅱ, respectively, in vitro. 1,4-Naphthoquinone derivatives with an amino group at the 2-position have been reported to have good antineoplasmic, carcinostatic actions' and bacterial growth inhibition. Based on the cytotoxic potential of 1,4-naphthoquinone derivatives, in our continuing efforts to develop novel antitumor agents, we have been synthesized benz[f]indole-4,9-dione analogs, heterocyclic pyrrole ring derivatives attached to 1,4-naphthoquinone. In this study we evaluated the cytotoxic potential of additional benz[f]indole-4,9-dione analogs in cultured human solid tumor cell lines including human lung (A549), colon (Col2), and stomach (SNU-638) cancer cells. Further, a variety of antitumor agents currently used in chemotherapy or evaluated in clinical trials are known to inhibit DNA topoisomerase Ⅰ (topo Ⅰ) or Ⅱ (topo Ⅱ). DNA topoisomerases are enzymes that catalyze the passage of individual DNA strands (type 1) or double helices (type Ⅱ) through one another, which is manifested in the interconversion between topological isomers of DNA. These enzymes have important roles in replication, recombination, transcription, chromosome condensation, and the maintenance of genome stability, and hence are good targets for antineoplastic drugs. The antitumor drugs camptothecin, doxorubicin, and etoposide are representative topo Ⅰ or topo Ⅱ inhibitors. Therefore, in this study, to investigate one possible mechanism of action of the cytotoxic activity of benz[f]indole-4,9-dione analogs, we evaluated their ability to inhibit topo Ⅰ or topo Ⅱ activities with DNA relaxation and a DNA decatenation assay, respectively. We report here that benz[f]-indole-4,9-dione analogs show potential cytotoxicity against cancer cell lines with topo Ⅰ or Ⅱ inhibitory activity.

건축물의 화재성상 시뮬레이션을 위한 주요가연물 연소특성 DB 구축

서윤정(Seo, Yoon-Jeong),김동은(Kim, Dong-Eun),홍해리(Hong, Hae-Ri),황현배(Hwang, Hyen-Bea),권영진(Kwon, Young-Jin) 한국화재소방학회 2011 한국화재소방학회 학술대회 논문집 Vol.2011 No.춘계

최근 건축물에서 공동주택의 비율이 증가하고 있으며 동시에 공동주택의 화재비율도 증가하는 경향을 보이고 있다. 또한 화재 시 인명 피해의 주원인인 가연물은 소재가 다양화되고 있으나 안정성 보다는 디자인, 기능성에 치중되어 그에 따른 화재 위험성도 커져가고 있다. 따라서 본 연구는 전보의 실내장식물에 이어 공동주택에서 많이 사용되어지는 적재가연물을 가연물 조사를 바탕으로 선정하여 Furniture Calorimeter를 이용해 그 연소특성을 알아보았다. 그 결과, 소파가 타 시료에 비해 화재 시 높은 위험성을 나타냈으며, 일본건축학회에 발표된 데이터와 비교한 결과 소파의 데이터가 유사한 것으로 나타났다.

Cytotoxic and Antineoplastic Activity of Timosaponin A-III for Human Colon Cancer Cells

Kang, You-Jin,Chung, Hwa-Jin,Nam, Joo-Won,Park, Hyen Joo,Seo, Eun Kyoung,Kim, Yeong Shik,Lee, Dongho,Lee, Sang Kook American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.4

<P>The potential antitumor activity of timosaponin A-III (<B>1</B>), a steroidal saponin from the rhizomes of <I>Anemarrhena asphodeloides</I>, was investigated in human colorectal cancer HCT-15 cells both in cell culture and in an in vivo murine xenograft model. Compound <B>1</B> inhibited the proliferation of cancer cells with cell-cycle arrest and induction of apoptosis. Cell-cycle arrest in the G0/G1 and G2/M phase by <B>1</B> was correlated with the down-regulation of cyclin A, cyclin B1, cyclin-dependent kinase 2 (CDK2), CDK4, proliferating cell nuclear antigen, and c-Myc. The increase of the sub-G1 peak by <B>1</B> was also closely related to the induction of apoptosis, which was evidenced by the induction of DNA fragmentation, activation of caspases, induction of cleaved poly-(ADP ribose) polymerase, and suppression of Bcl-xL and Bcl-2 expression. In an in vivo xenograft model, treatment with <B>1</B> (2 or 5 mg/kg body weight, three times/week, ip administration) for four weeks significantly suppressed tumor growth in athymic nude mice bearing HCT-15 cells, without any overt toxicity. Cell-cycle arrest and induction of apoptosis might be plausible mechanisms of actions for the observed antineoplastic activity of <B>1</B>.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-4/np1007735/production/images/medium/np-2010-007735_0006.gif'></P>

Serum cardiac troponin I concentrations in clinically normal and Dirofilaria immitis infected Korean Jindo dogs

Hwang, Soo-Hyen,Park, Hyung-Jin,Seo, Kyoung-Won,Lee, Sang-Eun,Song, Kun-Ho The Korean Society of Veterinary Science 2014 大韓獸醫學會誌 Vol.54 No.1

This study was conducted to determine the serum cardiac troponin I (cTnI) concentrations in clinically normal (uninfected) Korean Jindo dogs and those infected with Dirofilaria (D.) immitis. Forty Korean Jindo dogs (22 females, 18 males) were obtained from two kennels in the Boryeong area of Chungnam Province and the Daejeon area, Korea. Significantly higher cTnI concentrations were observed in D. immitis-infected dogs than clinically normal dogs. cTnI testing in addition to thoracic radiography, echocardiography, and electrocardiography shows promise as an additional method for diagnosis of heartworm infection.

Demonstration of Two Independent Dextranase and Amylase Active Sites on a Single Enzyme Elaborated by Lipomyces starkeyi KSM 22

LEE, SO-YOUNG,LEE, JIN-HA,ROBYT, JOHN F.,SEO, EUN-SEONG,PARK, HYEN-JOUNG,KIM, DOMAN 한국미생물 · 생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.2

Lipomyces starkeyi KSM 22 elaborates an enzyme that has both dextranase and amylase activities in a single protein of 100 kDa. Competition studies, using different amounts of dextran and starch as substrates, gave a competition plot consistent with the hypothesis that the hydrolysis of dextran and starch occurs at two independent active sites, each specific for starch and dextran, respectively.

Synthesis and cytotoxicity evaluation of substituted pyridazino[4,5-b] phenazine-5,12-diones and tri/tetra-azabenzofluorene-5,6-diones

Lee, Hyun-Jung,Kim, Jin-Sung,Suh, Myung-Eun,Park, Hyen Joo,Lee, Sang Kook,Rhee, Hee-Kyung,Kim, Hwa Jung,Seo, Eun-Kyung,Kim, Choonmi,Lee, Chong-Ock,Choo Park, Hea-Young 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.18

The substituted pyridazino[4,5-b]phenazine-5,12-diones and tri/tetra-azabenzo[a]fluorene-5,6-diones were synthesized from 6,7-dichloroph-thalazine-5,8-dione and 6,7-dichloroquinoline-5,8-dione, respectively. The cytotoxic activities of the prepared compounds were evaluated by an SRB (Sulforhodamine B) assay against the following human cancer cell lines: A549 (lung), SK-0V-3 (ovarian), SK-MEL-2 (melanoma), XF 498 (CNS), and HCT 15 (colon). Almost all synthesized pyridazino[4,5-b]phenazine-5,12-diones (7a-j) presented higher cytotoxicity than that of doxorubicin (IC_(50) = 0.097-0.225 μM) against the cancer cell lines, In particular, the cytotoxicity of compounds 7f (R₁=Et) and 7h (R₁, R₂ = Me) against all human cancer cell lines examined was about 10 times higher than that of doxorubicin. However, the cytotoxicities of several synthesized azabenzo[a]fluorene-5,6-diones (12a, 12c, 12d, 12e, and 12g) against the cancer cell lines in vitro were comparable to those of doxorubicin.

동시베리아해 비구형 망가니즈단괴의 특징

서충만(Choong Man Seo),박무성(Mu Seong Park),구효진(Hyo Jin Koo),조현구(Hyen Goo Cho) 대한지질학회 2021 대한지질학회 학술대회 Vol.2021 No.10

미래의 유망 자원으로 주목받고 있는 망가니즈단괴는 태평양이나 인도양의 심해저뿐만 아니라 북극해 여러 곳에서도 발견되고 있다. 망가니즈단괴는 성장 과정에 따라 외형, 내부조직, 화학조성 및 광물조성 등 여러 가지 특징이 달라진다. 망가니즈단괴의 성장 과정에 따른 내부조직과 화학조성은 다양한 연구를 통해 비교적 잘 알려져 있으나, 단괴를 구성하는 산화망가니즈광물의 입자가 매우 작고 결정도가 낮아서, 정량적인 광물조성은 분명하게 밝혀진 바가 없다. 이번 연구에서는 북극 동시베리아해 망가니즈단괴 중 비구형 망가니즈단괴의 외형과 내부조직을 관찰하고, X선회절 반정량 분석법을 적용하여 망가니즈단괴를 구성하는 3가지 주요한 산화망가니즈광물 즉, 토도로카이트, 부서라이트, 버네사이트의 함량비를 결정하였다. 이와 같은 결과를 이용하여 외형이나 내부조직과 광물조성 사이의 상관관계를 알아보고, 구형 망가니즈단괴와 비교하였다. 동시베리아해 비구형 망가니즈단괴를 구성하는 산화망가니즈광물 중 버네사이트의 함량이 가장 높고(평균 64.4%), 토도로카이트가 가장 낮다(평균 15.3%). 광물조성과 내부조직과는 뚜렷한 연관성을 나타내지 않지만, 토도로카이트 함량이 단괴 내부(평균 27.4%)에서 표면(평균 3.3%)으로 갈수록 감소하는 경향을 보인다. 비구형 망가니즈단괴는 구형보다 더 크고 무거운 경향을 보이며, 핵이 거의 존재하지 않던 구형과 달리 비구형 단괴에서는 대부분 핵이 확인되었으며, 규산염광물(석영, 장석 등)과 백운석으로 구성되어 있다. Mn/Fe비는 구형 단괴(21.7)보다 비구형 단괴(7.0)에서 더 낮았지만, 광물조성이나 내부조직에서는 큰 차이점을 보이지 않는다.

Aspergillus nidulans 의 tRNA 유전자의 구조와 발현에 관한 연구 ( Ⅶ ) Aspergillus nidulans의 initiator methionyl - tRNA 유전자의 분자구조

서진영,오상진,강현삼 ( Jin Young Seo,Sang Jin Oh,Hyen Sam Kang ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.3

A specific tRNA gene clone (named pANt86) was isolated by colony hybridization to the total tRNA gene bank with a certain tRNA probe extracted from Aspergillus nidulans. The 485 nucleotide long sequence containing this gene was determined by the Maxam and Gilbert sequencing method. The sequence analysis reveals that this tRNA is methionyl-tRNA(CAU). Compared with other initiator tRNA^(Met) gene for the sequence homology and two-positional discriminators, this tRNA gene is initiator tRNA^(Met). The nucleotide sequences of this tRNA gene was compared with those of other organisms. From this, the intragenic control regions constituted by two separate transcriptional signals(Box A and Box B) are located in the regions of highly conserved sequences. And this gene reveals several conserved upstream sequences, the ACT-TA box, TA(C)TAT, AATTTT, and TTC. The T clusters are also found in the 3`-flanking region. And an evolutionary distance matrix was obtained and a phylogenetic tree based on this matrix was constructed. This phylogenetic tree reveals that extant organisms are found to fall into three phylogenetically coherent groups, the eukaryotes, the eubacteria, and the archaebacteria.

The Survey of Recognition about Rehabilitative Robots for Treatmentin Physical Therapists

( Hyosuk Kim ),( Dong Jin Kang ),( Deok Hyen Kim ),( Seo Jeong Park ),( Seong Yong Lee ),( Jeong Min Lee ),( Seung Yeon Jo ),( Bo Ram Choi ),( Minhee Kim ) 대한물리치료학회 2021 대한물리치료학회지 Vol.33 No.2

Purpose: This study examined the recognition of rehabilitative robots for treatment in physical therapists. Methods: This study surveyed 100 physical therapists in Seoul and Gyeonggi-do using Google Form, an online survey tool. The questionnaire consisted of 21 questions, including eight questions on the general characteristics, 13 questions on the recognition of rehabilitative robots. Results: The general characteristics of the physical therapists showed differences and influences on recognition of rehabilitative robots, and there were statistically significant differences. There were significant differences in the recognition of rehabilitation robots according to general characteristics in gender, age, education degree, type of hospital, average weekly working time, and treatment field. Multiple regression analysis found that gender and the type of hospital influenced the recognition of rehabilitation robots. Conclusion: Physical therapists showed differences in recognition of rehabilitative robots according to their general characteristics, and gender and the type of hospital influence the recognition of rehabilitation robots. Sufficient systematic education programs should be provided, and physical therapists require policy adjustments to increase their accessibility to rehabilitation robots through continuing education.