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RISS 인기검색어
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- 저자 : 강현삼 ( Jin Young Seo (검색결과 2 건)
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서진영,오상진,강현삼,Seo, Jin-Young,Oh, Sang-Jin,Kang, Hyen-Sam 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.3
Aspergillus nidulans로부터 추출한 특정 tRNA를 참침으로 삼아, tRNA 유전자 은행으로부터 이 tRNA의 유전자를 갖고 있는 클론인 pANt86을 찾았다. Maxam과 Gilbert의 방법으로 이 유전자를 포함한 485개 염기의 서열을 결정하였다. 이 자료를 컴퓨터 프로그램으로 분석한 결과, anticodon이 CAU인 methionyl-tRNA의 유전자가 존재하였다. 유전자 염기 서열의 상동성 비교와 two-positionaldiscriminator의 공통성을 조사하여 이 tRNA 유전자가 initiator methionyl-tRNA 유전자임을 밝혔다. 이 tRNA 유전자도 Box A와 Box B의 conserved sequence로 구성된 intragenic control region (ICR)을 갖고 있었으며, 5'-flanking 부분에는 전사율을 조철하는 것으로 보이는 conserved sequence들이 존재하였다. 또 3'-flanking 부분에는 T cluster가 여럿 존재하는데, RNA polymerase III의 전사 종결에 관여할 것으로 보인다. 이 밖에도 tRNA 유전자의 염기 서열을 진화적 고찰에 이용하였다. 그 결과 크게 3가지 계 즉, archaebacteria, eubacteria, 진핵생물로 분류되었다. A specific tRNA gene clone (named pANt86) was isolated by colony hybridization to the total tRNA gene bank with a certain tRNA probe extracted from Aspergillus nidulans. The 485 nucleotide long sequence containing this gene was determined by the Maxam and Gilbert sequencing method. The sequence analysis reveals that this tRNA is methionyl-tRNA(CAU). Compared with other initiator $tRNA^{Met}$ gene for the sequence homology and two-positional discriminators, this tRNA gene is initiator $tRNA^{Met}$ The nucleotide sequences of this tRNA gene was compared with those of other organisms. From this, the intragenic control regions constituted by two separate transcriptional signals(Box A and Box B) are located in the regions of highly conserved sequences. And this gene reveals several conserved upstream sequences, the ACT-TA box, TA(C)TAT, AATTTT, and TTC. The T clusters are also found in the 3'-flanking region. And an evolutionary distance matrix was obtained and a phylogenetic tree based on this matrix was constructed. This phylogenetic tree reveals that extant organisms are found to fall into three phylogenetically coherent groups, the eukaryotes, the eubacteria, and the archaebacteria.
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서진영,오상진,강현삼 ( Jin Young Seo,Sang Jin Oh,Hyen Sam Kang ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.3
A specific tRNA gene clone (named pANt86) was isolated by colony hybridization to the total tRNA gene bank with a certain tRNA probe extracted from Aspergillus nidulans. The 485 nucleotide long sequence containing this gene was determined by the Maxam and Gilbert sequencing method. The sequence analysis reveals that this tRNA is methionyl-tRNA(CAU). Compared with other initiator tRNA^(Met) gene for the sequence homology and two-positional discriminators, this tRNA gene is initiator tRNA^(Met). The nucleotide sequences of this tRNA gene was compared with those of other organisms. From this, the intragenic control regions constituted by two separate transcriptional signals(Box A and Box B) are located in the regions of highly conserved sequences. And this gene reveals several conserved upstream sequences, the ACT-TA box, TA(C)TAT, AATTTT, and TTC. The T clusters are also found in the 3`-flanking region. And an evolutionary distance matrix was obtained and a phylogenetic tree based on this matrix was constructed. This phylogenetic tree reveals that extant organisms are found to fall into three phylogenetically coherent groups, the eukaryotes, the eubacteria, and the archaebacteria.
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