Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

Light Scattering Study of Glass Transition Temperatures of Glucose-Water Mixtures

서정아,오지영,김형국,황윤회 부산대학교 유전체물성연구소 2004 유전체 논문집 Vol.3 No.

We studied glass transition temperatures of glucose-water mixtures by using Brillouin light scattering. We fitted the polarized components of the Brillouin spectra measured by backscattering geometry to simple Lorentzian form and estimated the glass transition temperature from the slope of the temperature-dependent Brillouin peak shift.

진단 초음파를 이용한 정상 성인의 정중신경 측정

이정아,임길병,이홍재,정웅태,황현오 최신의학사 2003 最新醫學 Vol.46 No.11-12

좋은 죽음의 개념 분석

이경주,황경혜,라정란,홍정아,박재순 가톨릭대학교 간호대학 호스피스 교육연구소 2006 호스피스논집 Vol.10 No.-

Purpose: Death is not an option but a necessity in our life. However, there is little understanding of what a good death means. This study was conducted to analyze the concept of a good death by exploring attributes, antecedents, consequences and empirical criteria for a good death in literatures. Method : Reviewed materials were collected by searching for web cites such as PubMed, National Library of Korea, National Assembly Library. Walker and Avant's method(2004) was used for this concept analysis. Results : The findings showed that a good death includes seven attributes: dignity (personal respect and privacy), self-control (the right of choice, independence, clear consciousness), comfort (physical, psychological, spiritual). optimal relationships (supportive environment, proper care, restoration of relationships), appropriateness (sequence and proper age of death), preparation for death (completing unsettled tasks, leaving a legacy, positive attitude toward life, awareness and acceptance of death) and minimizing burden. Conclusion : This results will provide health professionals and people with a preliminary understandings of a good death. In addition, there is the need for further study to develop objective measurement of a good death and effective nursing intervention for the dying.

Liver imaging reporting and data system category on magnetic resonance imaging predicts recurrence of intrahepatic cholangiocarcinoma after surgery: A multicenter study

Jeong Ah HWANG,Sunyoung LEE,Ji Eun LEE 한국간담췌외과학회 2022 Annals of hepato-biliary-pancreatic surgery Vol.26 No.-

Total Bilirubin Level as a Predictor of Suboptimal Image Quality of the Hepatobiliary Phase of Gadoxetic Acid-Enhanced MRI in Patients with Extrahepatic Bile Duct Cancer

Hwang Jeong Ah,Min Ji Hye,Kim Seong Hyun,Choi Seo-Youn,Lee Ji Eun,Moon Ji Yoon 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.4

Objective: This study aimed to determine a factor for predicting suboptimal image quality of the hepatobiliary phase (HBP) of gadoxetic acid-enhanced MRI in patients with extrahepatic bile duct (EHD) cancer before MRI examination. Materials and Methods: We retrospectively evaluated 259 patients (mean age ± standard deviation: 68.0 ± 8.3 years; 162 male and 97 female) with EHD cancer who underwent gadoxetic acid-enhanced MRI between 2011 and 2017. Patients were divided into a primary analysis set (n = 184) and a validation set (n = 75) based on the diagnosis date of January 2014. Two reviewers assigned the functional liver imaging score (FLIS) to reflect the HBP image quality. The FLIS consists of the sum of three HBP features, each scored on a 0–2 scale: liver parenchymal enhancement, biliary excretion, and signal intensity of the portal vein. Patients were classified into low-FLIS (0–3) or high-FLIS (4–6) groups. Multivariable analysis was performed to determine a predictor of low FLIS using serum biochemical and imaging parameters of cholestasis severity. The optimal cutoff value for predicting low FLIS was obtained using receiver operating characteristic analysis, and validation was performed. Results: Of the 259 patients, 140 (54.0%) and 119 (46.0%) were classified into the low-FLIS and high-FLIS groups, respectively. In the primary analysis set, total bilirubin was an independent factor associated with low FLIS (adjusted odds ratio per 1-mg/dL increase, 1.62; 95% confidence interval [CI], 1.32–1.98). The optimal cutoff value of total bilirubin for predicting low FLIS was 2.1 mg/dL with a sensitivity of 95.1% (95% CI: 88.9–98.4) and a specificity of 89.0% (95% CI: 80.2–94.9). In the validation set, the total bilirubin cutoff showed a sensitivity of 92.1% (95% CI: 78.6–98.3) and a specificity of 83.8% (95% CI: 68.0–93.8). Conclusion: Serum total bilirubin before acquisition of gadoxetic acid-enhanced MRI may help predict suboptimal HBP image quality in patients with EHD cancer.

Wnt5a-induced docking of Plk1 on HEF1 promotes HEF1 translocation and tumorigenesis

Jeong-Ah Hwang,Ji Eun Yu,Sun-Ok Kim,Dong Hyun Kim,조경상,이경호 한국유전학회 2021 Genes & Genomics Vol.43 No.5

Background Upregulation of human enhancer flamentation 1 (HEF1/NEDD9/Cas-L) and Polo-like kinase 1 (Plk1) is closely correlated with metastasis of human cancer. However, the mechanism by which the overexpression of HEF1 or Plk1 stimulates cancer metastasis and induces tumorigenesis remains enigmatic. In addition, the accumulation of HEF1 at the focal adhesion (FA) is known to be an essential event in cancer cell migration, but the mechanism of how HEF1 is targeted to the FA remains yet to be unveiled. Objective This study was performed to elucidate the FA docking mechanism of HEF1 and to determine its efect on tumorigenesis. Methods To confrm the efect of the kinase on HEF1 translocation, various expression-knockdown stable cell lines were generated using a lentivirus system, and the efect of the HEF1-Plk1 complex on tumorigenesis was confrmed using a xenograft mouse model. Results Here, we show that Wnt5a-dependent Plk1 binding to HEF1 is critically required for HEF1 translocation to the FA. We also confrmed that Plk1 and CK1δ activities essential for HEF1 translocation are induced by Wnt5a. Finally, we confrmed the induction of tumorigenesis by the HEF1-Plk1 complex in the xenograft mouse model. Conclusion Our data collectively unveil the Wnt5a-CK1δ-HEF1-Plk1-FA remodeling pathway that governs HEF1 transportation to the FA to induce cell migration and tumorigenesis. This study sheds light on a mechanism underlying tumorigenesis and provides new strategies for anticancer therapy.

미얀마 NGO 활동가들의 젠더교육과 실천 - 까친주 활동가들의 경험을 중심으로

황정아 ( Hwang Jeong Ah ),신지원 ( Shin Julia Jiwon ) 부산대학교 여성연구소 2021 여성학연구 Vol.31 No.1

이 연구는 미얀마 까친주 지역 NGO 활동가들의 젠더교육 경험을 중심으로 지방, 소수민족 그리고 IDP 캠프라는 분쟁의 공간에서 실천되고 있는 젠더교육이 대도시인 양곤과 어떻게 연계되어 있고 어떠한 차이를 함의하고 있는지를 분석하고, 지역 내 젠더교육의 실천과정에서 지역활동가들이 겪는 문제를 조명한다. 이 연구는 또한 국제원조의 유입과 미얀마의 여성운동 안에서 이루어지고 있는 여성NGO의 젠더교육 확산과정을 탈식민주의 페미니즘의 맥락에서 분석한다. 135개의 소수민족이 연방을 이루고 있는 미얀마는 문화와 관습, 언어의 차이, 민족 간 분쟁으로 인해 여성운동 내부에서 차이의 문제는 민감하고 중요한 사안이다. 그러나 이 연구에서 살펴본 까친주에서 이루어지는 젠더교육은 지역, 계층, 민족, 분쟁, IDP와 같은 차이의 변수들이 충분히 다루어지지 않고, 국제사회의 여성개발담론의 틀 안에서 벗어나지 않는 보편적 권리담론에 치우쳐 탈정치화되어있다. 이는 국제사회의 성평등 원조사업이 양곤의 단체들의 주도하에 지역으로 전달되는 수직적 흐름과 연관되어 있다. 미얀마의 젠더교육은 서구의 발전담론의 틀과 민족국가의 제도 안에서 이루어지고 있으며, 지역활동가들은 복지서비스의 전달자로서 역할이 더 두드러져 보인다. 따라서 여성 억압의 교차적 요인들을 고려한 다양한 측면에서의 젠더교육이 요구된다. Based on the qualitative analysis of the experience of NGO activists in Kachin State, this study examines the process of expanding gender education of women's NGOs in Myanmar. It critically examines the ways in which gender educations are conducted in the various spaces of states, districts, and IDP camps, and connected to international organizations and a metropolitan city, Yangon. According to the findings of the study, gender education in Myanmar is depoliticized based on the universal rights discourse that does not sufficiently deal with the variables of differences among women, such as class, ethnicity, conflict situations and IDPs. This is related to the vertical flow of the international community's aid projects to the region, led by organizations in Yangon. Myanmar's gender education is being carried out within the framework of the Western development discourse and the system of the nation-state, and the local NGO’s role as a mere provider of welfare services appears to be more prominent. Gender education is, therefore, required to consider the intersectional factors of women's oppression, including localities, poverty, ethnicities, and socio-economic positions.

냉동 저장 온도가 쇠고기의 저장성에 미치는 영향

박정아(Jeong-Ah Park),주소영(So Young Joo),황현정(Hyun Jung Hwang),나예슬(Ye Seul Na),김서진(Seo Jin Kim),최정인(Jeong In Choi),하주영(Joo Young Ha),조미숙(Mi Sook Cho) 한국식품과학회 2016 한국식품과학회지 Vol.48 No.4

본 연구는 쇠고기의 단기 저장 중 이화학 분석을 통하여 가정에서 쇠고기 저장 시 냉동 저장 조건을 탐색해보았다. 쇠고기의 지방 산패 정도를 나타내는 TBA가에서는 −5℃ 저장군이 −20℃ 저장군과 유사한 경향을 보였으며, 쇠고기의 단백질 신선도를 나타내는 VBN 함량과 일반세균수도 −5℃ 저장군과 −20℃ 저장군이 유사한 추세를 보이며 증가하였다. 적색도와 전단력에서는 −5℃ 저장군이 −20℃ 저장군보다 −1℃ 저장군과 흡사한 경향을 보였다. 이에 대해 식품의 유통기한에 대한 식품의약품안전처의 고시(2014)에 따르면 식육의 설정실험 지표는 TBA가, 휘발성염기질소, 세균수, 대장균수이므로 본 실험의 TBA가와 VBN 함량, 일반세균 수 항목에서 −5℃에서의 저장이 −20℃ 저장과 유사한 결과를 나타낸 점에 의의가 있다. 이상의 연구결과를 종합해 볼 때, 쇠고기의 단기 저장 시 −5℃에서의 저장은 −20℃에서의 저장과 유사한 측면을 보이며 약 52일간 저장이 가능할 것으로 예측되어 쇠고기의 단기 저장 시 신선도 유지에 효율적이라고 생각된다. 추후 온도 조절뿐만 아니라 가정용 냉장고의 습도, 쇠고기의 포장 방법, 저장 시 용량 등 여러 인자들의 조절로 가정 내 쇠고기의 냉동 저장에 대한 연구가 필요할 것으로 보인다. This study was conducted to investigate changes in pH, volatile basic nitrogen (VBN), thiobarbituric acid (TBA), a* (redness), shearing force, and microbial content (total plate count; TPC) in beef during freezing storage at −1, −5, and −20℃ for 21 days. TPC as an effective quality indicator was used to identify the decay point by regression analysis. The pH, TBA values, and TPC significantly increased with storage at all tested temperatures (p<0.05). VBN significantly increased and shearing force significantly decreased with storage at −1 and −5℃ (p<0.05). TPC showed significant correlation with temperature at −1℃ (R²=0.891), −5℃ (R2=0.856), and −20℃ (R²=0.444). The decay points at −1, −5, and −20℃, were 27, 52, and 84 days, respectively. The results suggest that a freezing temperature of −5℃ is effective for short-term storage of beef.

Distinct roles of two ceramide synthases, CaLag1p and CaLac1p, in the morphogenesis of <i>Candida albicans</i>

Cheon, Seon Ah,Bal, Jyotiranjan,Song, Yunkyoung,Hwang, Hai‐,min,Kim, Ah Ruem,Kang, Woo Kyu,Kang, Hyun Ah,Hannibal‐,Bach, Hans K.,Knudsen, Jens,Ejsing, Christer S.,Kim, Jeong‐,Yoon Blackwell Publishing Ltd 2012 Molecular microbiology Vol.83 No.4

<P><B>Summary</B></P><P>Lag1p and Lac1p catalyse ceramide synthesis in <I>Saccharomyces cerevisiae.</I> This study shows that Lag1 family proteins are generally required for polarized growth in hemiascomycetous yeast. However, in contrast to <I>S. cerevisiae</I> where these proteins are functionally redundant, <I>C. albicans</I> Lag1p (CaLag1p) and Lac1p (CaLac1p) are functionally distinct. Lack of CaLag1p, but not CaLac1p, caused severe defects in the growth and hyphal morphogenesis of <I>C. albicans</I>. Deletion of <I>CaLAG1</I> decreased expression of the hypha‐specific <I>HWP1</I> and <I>ECE1</I> genes. Moreover, overexpression of <I>CaLAG1</I> induced pseudohyphal growth in this organism under non‐hypha‐inducing conditions, suggesting that CaLag1p is necessary for relaying signals to induce hypha‐specific gene expression. Analysis of ceramide and sphingolipid composition revealed that CaLag1p predominantly synthesizes ceramides with C24:0/C26:0 fatty acid moieties, which are involved in generating inositol‐containing sphingolipids, whereas CaLac1p produces ceramides with C18:0 fatty acid moieties, which are precursors for glucosylsphingolipids. Thus, our study demonstrates that CaLag1p and CaLac1p have distinct substrate specificities and physiological roles in <I>C. albicans</I>.</P>