Characterization of culturable yeast species associating with whole crop corn and total mixed ration silage

Huili Wang,Wei Hao,Tingting Ning,Mingli Zheng,C.C. Xu 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: This study investigated the association of yeast species with improved aerobic stability of total mixed ration (TMR) silages with prolonged ensiling, and clarified the characteristics of yeast species and their role during aerobic deterioration. Methods: Whole crop corn (WCC) silages and TMR silages formulated with WCC were ensiled for 7, 14, 28, and 56 d and used for an aerobic stability test. Predominant yeast species were isolated from different periods and identified by sequencing analyses of the 26S rRNA gene D1/D2 domain. Characteristics (assimilation and tolerance) of the yeast species and their role during aerobic deterioration were investigated. Results: In addition to species of Candida glabrata and Pichia kudriavzevii (P. kudriavzevii) previously isolated in WCC and TMR, Pichia manshurica (P. manshurica), Candida ethanolica (C. ethanolica), and Zygosaccharomyces bailii (Z. bailii) isolated at great frequency during deterioration, were capable of assimilating lactic or acetic acid and tolerant to acetic acid and might function more in deteriorating TMR silages at early fermentation (7 d and 14 d). With ensiling prolonged to 28 d, silages became more (p<0.01) stable when exposed to air, coinciding with the inhibition of yeast to below the detection limit. Species of P. manshurica that were predominant in deteriorating WCC silages were not detectable in TMR silages. In addition, the predominant yeast species of Z. bailii in deteriorating TMR silages at later fermentation (28 d and 56 d) were not observed in both WCC and WCC silages. Conclusion: The inhibition of yeasts, particularly P. kudriavzevii, probably account for the improved aerobic stability of TMR silages at later fermentation. Fewer species seemed to be involved in aerobic deterioration of silages at later fermentation and Z. bailii was most likely to initiate the aerobic deterioration of TMR silages at later fermentation. The use of WCC in TMR might not influence the predominant yeast species during aerobic deterioration of TMR silages.

Dynamics Associated with Prolonged Ensiling and Aerobic Deterioration of Total Mixed Ration Silage Containing Whole Crop Corn

Wang, Huili,Ning, Tingting,Hao, Wei,Zheng, Mingli,Xu, Chuncheng Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.1

This study investigated the dynamics associated with prolonged ensiling and aerobic deterioration of whole crop corn (WCC) silages and total mixed ration (TMR) silages containing WCC (C-TMR silages) to clarify the differences that account for the enhanced aerobic stability of TMR silages. Laboratory-scale barrel silos were randomly opened after 7, 14, 28, and 56 d of ensiling and were subjected to analyses of fermentation quality, microbial and temperature dynamics during aerobic exposure. WCC and C-TMR silages were both well preserved and microorganisms were inhibited with prolonged ensiling, including lactic acid bacteria. Yeast were inhibited to below the detection limit of 500 cfu/g fresh matter within 28 d of ensiling. Aerobic stability of both silages was enhanced with prolonged ensiling, whereas C-TMR silages were more aerobically stable than WCC silages for the same ensiling period. Besides the high moisture content, the weak aerobic stability of WCC silage is likely attributable to the higher lactic acid content and yeast count, which result from the high water-soluble carbohydrates content in WCC. After silo opening, yeast were the first to propagate and the increase in yeast levels is greater than that of other microorganisms in silages before deterioration. Besides, increased levels of aerobic bacteria were also detected before heating of WCC silages. The temperature dynamics also indicated that yeast are closely associated with the onset of the aerobic deterioration of C-TMR silage, whereas for WCC silages, besides yeast, aerobic bacteria also function in the aerobic deterioration. Therefore, the inclusion of WCC might contribute to the survival of yeast during ensiling but not influence the role of yeast in deterioration of C-TMR silages.

Effects of microbial enzymes on starch and hemicellulose degradation in total mixed ration silages

Ning, Tingting,Wang, Huili,Zheng, Mingli,Niu, Dongze,Zuo, Sasa,Xu, Chuncheng Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

Objective: This study investigated the association of enzyme-producing microbes and their enzymes with starch and hemicellulose degradation during fermentation of total mixed ration (TMR) silage. Methods: The TMRs were prepared with soybean curd residue, alfalfa hay (ATMR) or Leymus chinensis hay (LTMR), corn meal, soybean meal, vitamin-mineral supplements, and salt at a ratio of 25:40:30:4:0.5:0.5 on a dry matter basis. Laboratory-scale bag silos were randomly opened after 1, 3, 7, 14, 28, and 56 days of ensiling and subjected to analyses of fermentation quality, carbohydrates loss, microbial amylase and hemicellulase activities, succession of dominant amylolytic or hemicellulolytic microbes, and their microbial and enzymatic properties. Results: Both ATMR and LTMR silages were well preserved, with low pH and high lactic acid concentrations. In addition to the substantial loss of water soluble carbohydrates, loss of starch and hemicellulose was also observed in both TMR silages with prolonged ensiling. The microbial amylase activity remained detectable throughout the ensiling in both TMR silages, whereas the microbial hemicellulase activity progressively decreased until it was inactive at day 14 post-ensiling in both TMR silages. During the early stage of fermentation, the main amylase-producing microbes were Bacillus amyloliquefaciens (B. amyloliquefaciens), B. cereus, B. licheniformis, and B. subtilis in ATMR silage and B. flexus, B. licheniformis, and Paenibacillus xylanexedens (P. xylanexedens) in LTMR silage, whereas Enterococcus faecium was closely associated with starch hydrolysis at the later stage of fermentation in both TMR silages. B. amyloliquefaciens, B. licheniformis, and B. subtilis and B. licheniformis, B. pumilus, and P. xylanexedens were the main source of microbial hemicellulase during the early stage of fermentation in ATMR and LTMR silages, respectively. Conclusion: The microbial amylase contributes to starch hydrolysis during the ensiling process in both TMR silages, whereas the microbial hemicellulase participates in the hemicellulose degradation only at the early stage of ensiling.

Characteristics of proteolytic microorganisms and their effects on proteolysis in total mixed ration silages of soybean curd residue

Hao, Wei,Tian, Pengjiao,Zheng, Mingli,Wang, Huili,Xu, Chuncheng Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1

Objective: The objective of this study was to isolate proteolytic microorganisms and evaluate their effects on proteolysis in total mixed ration (TMR) silages of soybean curd residue. Methods: TMRs were formulated with soybean curd residue, alfalfa or Leymus chinensis hay, corn meal, soybean meal, a vitamin-mineral supplement, and salt in a ratio of 25.0: 40.0:30.0:4.0:0.5:0.5, respectively, on a basis of dry matter. The microbial proteinases during ensiling were characterized, the dominate strains associated with proteolysis were identified, and their enzymatic characterization were evaluated in alfalfa (A-TMR) and Leymus chinensis (L-TMR) TMR silages containing soybean curd residue. Results: Both A-TMR and L-TMR silages were well preserved, with low pH and high lactic acid concentrations. The aerobic bacteria and yeast counts in both TMR silages decreased to about 10⁵ cfu/g fresh matter (FM) and below the detection limit, respectively. The lactic acid bacteria count increased to 10⁹ cfu/g FM. The total microbial proteinases activities reached their maximums during the early ensiling stage and then reduced in both TMR silages with fermentation prolonged. Metalloproteinase was the main proteinase when the total proteinases activities reached their maximums, and when ensiling terminated, metallo and serine proteinases played equally important parts in proteolysis in both TMR silages. Strains in the genera Curtobacterium and Paenibacillus were identified as the most dominant proteolytic bacteria in A-TMR and L-TMR, respectively, and both their proteinases were mainly with metalloproteinase characteristics. In the latter ensiling phase, Enterococcus faecium strains became the major sources of proteolytic enzymes in both TMR silages. Their proteinases were mainly of metallo and serine proteinases classes in this experiment. Conclusion: Proteolytic aerobic bacteria were substituted by proteolytic lactic acid bacteria during ensiling, and the microbial serine and metallo proteinases in these strains played leading roles in proteolysis in TMR silages.

Fermentation Characteristics and Lactic Acid Bacteria Succession of Total Mixed Ration Silages Formulated with Peach Pomace

Xiaodong Hu,Wei Hao,Huili Wang,Tingting Ning,Mingli Zheng,C.C. Xu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4

The objective of this study was to assess the use of peach pomace in total mixed ration (TMR) silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB). The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM) basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.

Fermentation Characteristics and Lactic Acid Bacteria Succession of Total Mixed Ration Silages Formulated with Peach Pomace

Hu, Xiaodong,Hao, Wei,Wang, Huili,Ning, Tingting,Zheng, Mingli,Xu, Chuncheng Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.4

The objective of this study was to assess the use of peach pomace in total mixed ration (TMR) silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB). The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM) basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.

Long-Term Trends in Ischemic Stroke Incidence and Risk Factors: Perspectives from an Asian Stroke Registry

Benjamin Y.Q. Tan,Joshua T.C. Tan,Dawn Cheah,Huili Zheng,Pin Pin Pek,Deidre A. De Silva,Aftab Ahmad,Bernard P.L. Chan,Hui Meng Chang,Keng He Kong,Sherry H. Young,Kok Foo Tang,Tian Ming Tu,Leonard Leon 대한뇌졸중학회 2020 Journal of stroke Vol.22 No.3

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Bearing fault diagnosis based on amplitude and phase map of Hermitian wavelet transform

Hui Li,Lihui Fu,Haiqi Zheng 대한기계학회 2011 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.25 No.11

The rolling element bearing characteristic frequencies contain very little energy and are usually overwhelmed by noise and higher level of structural vibrations. The continuous wavelet transform enables one to look at the evolution in the time scale joint representation plane. This makes it very suitable for the detection of singularity generated by localized defects in a mechanical system. However, most applications of the continuous wavelet transform have widely focused on the use of the Morlet wavelet transform. The complex Hermitian wavelet is constructed based on the first and the second derivatives of the Gaussian function to detect signal singularities. The Fourier spectrum of Hermitian wavelet is real, which the Fourier spectrum has no complex phase and the Hermitian wavelet does not affect the phase of a signal in complex domain. This gives the desirable ability to detect the singularity characteristic of a signal precisely. In this study, the Hermitian wavelet amplitude and phase map are used in conjunction to detect and diagnose the bearing fault. The Hermitian wavelet amplitude and phase map are found to show distinctive signatures in the presence of bearing inner race or outer race damage. The simulative and experimental results show that the Hermitian wavelet amplitude and phase map can extract the transients from strong noise signals and can effectively diagnose bearing faults.

Theoretical and experimental investigation of the effects of an irregular-pitch cutter on vibration in face-milling

Hui Li,Haiqi Zheng,Liwei Tang 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.11

In the present work, cutting-force models of single-tooth and multi-teeth face-milling cutters were set up. Based on a spectrum analysis of cutting force, the vibration mechanism of a face-milling cutter of irregular pitch was investigated theoretically. The single-objective function and constraint conditions were derived. A general-purpose irregular-pitch face-milling cutter subsequently was designed and tested, and its performance was compared with that of a regular-pitch cutter. The experimental results showed that the irregular-pitch face-milling cutter not only reduces vibration and noise but also enhances surface-finish quality.

Gear fault detection and diagnosis under speed-up condition based on order cepstrum and radial basis function neural network

Hui Li,Yuping Zhang,Haiqi Zheng 대한기계학회 2009 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.23 No.10

Varying speed machinery condition detection and fault diagnosis are more difficult due to non-stationary machine dynamics and vibration. Therefore, most conventional signal processing methods based on time invariant carried out in constant time interval are frequently unable to provide meaningful results. In this paper, a study is presented to apply order cepstrum and radial basis function (RBF) artificial neural network (ANN) for gear fault detection during speedup process. This method combines computed order tracking, cepstrum analysis with ANN. First, the vibration signal during speed-up process of the gearbox is sampled at constant time increments and then is re-sampled at constant angle increments. Second, the re-sampled signals are processed by cepstrum analysis. The order cepstrum with normal, wear and crack fault are processed for feature extracting. In the end, the extracted features are used as inputs to RBF for recognition. The RBF is trained with a subset of the experimental data for known machine conditions. The ANN is tested by using the remaining set of data. The procedure is illustrated with the experimental vibration data of a gearbox. The results show the effectiveness of order cepstrum and RBF in detection and diagnosis of the gear condition.