MUC16 facilitates cervical cancer progression via JAK2/STAT3 phosphorylation‑mediated cyclooxygenase‑2 expression

Hui Shen,Meng Guo,Lu Wang,Xinyue Cui 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Objectives MUC16 (mucin 16, also known as CA-125, cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) has been predicted as tumor biomarker for therapy. We determined to investigate effects and regulatory mechanism of MUC16 on cervical tumorigenesis. Methods Expression levels of MUC16 in cervical cancer cell lines was analyzed via qRT-PCR (quantitative real-time polymerase chain reaction). Knockdown of MUC16 was conducted via shRNA (Short hairpin RNA) transfection. MTT and colony formation assays were used to investigate effect of MUC16 on cell proliferation. Wound healing assay was utilized to detect migration and transwell assay to detect invasion. The underlying mechanism was demonstrated via western blot analysis. Results MUC16 was elevated in cervical cancer cell lines. MUC16 knockdown inhibited cell proliferation, invasion and migration. Gain- and loss-of functional assays revealed that over-expression of MUC16 activated Janus Kinase 2 (JAK2)/ signal transducer and activator of transcription 3 (STAT3) via phosphorylation, thus facilitating cyclooxygenase-2 (COX-2) expression, while knockdown of MUC16 demonstrated the reverse effect on JAK2/STAT3 activation and COX-2 expression. Moreover, inhibition of JAK2/STAT3 attenuated the regulation of MUC16 on COX-2. Conclusions MUC16 enhanced proliferation and invasion of cervical cancer cells via JAK2/STAT3 phosphorylation-mediated cyclooxygenase-2 expression, suggesting the potential therapeutic target ability of MUC16 to treat cervical cancer.

적응적 방법에 의한 하이퍼큐브 네트워크 결함 진단 알고리즘

최혜연,김선신,강성수,최길호,이충세 충북대학교 컴퓨터정보통신 연구소 2003 컴퓨터정보통신연구 Vol.11 No.2

시스템 레벨의 대부분의 진단 알고리즘은 PMC 모델을 바탕으로 결함의 개수가 t개를 초과하지 않는다는 t-진단가능 시스템의 특성을 이용하여 결함을 진단한다. 그러나, 다중처리 시스템 상에서 큰 규모의 결함 집합을 고려할 때에 여러 가지 제약이 따른다. 또한 병렬처리 시스템의 규모가 커짐에 따라 시스템 내에서 발생되는 결함의 빈도가 높아지게 된다. 진단 알고리즘에서 가정하는 결함의 개수 t는 병렬처리 시스템 안에 있는 노드의 수에 비해 상당히 작은 개수이며, 결함의 개수가 t개를 초과할 경우의 연구는 거의 이루어지지 않았다. Somani와 Peleg은 k개의 부정확한 진단을 용인함으로써 결함의 개수가 t개를 초과할 경우에도 시스템을 진단하는 t/k-dignosable 시스템을 제안하였다. 이 논문에서는 Somani 등이 제안한 것처럼 k=1개의 부정확한 진단을 용인하는 경우에 하이퍼큐브를 진단하는 알고리즘을 제안한다. 이 논문에서 제안한 알고리즘은 더 많은 결함을 진단하면서도 기존의 알고리즘보다 효율이 거의 떨어지지 않는다는 사실을 분석을 통하여 확인할 수 있었다. System level diagnosis algorithms are based on the PMC model and use the properties of t-diagnosable system where the maximum number of the faults does not exceed t. Diagnostic algorithms have limit when dealing with large fault sets in multiprocessor systems. Somani and Peleg proposed t/k-diagnosable system to system diagnose by allowing upper bounded few number of units to be diagnosed incorrectly, which can diagnosis more faults than t-diagnosable system. In this paper, we propose hypercube diagnosis algorithm using t/k-diagnosable system. When the number of faults exceeds t, we allow a fault(k=1) to be diagnosed incorrectly, Based on this idea, we find that the performance of the proposed algorithm is nearly as efficient as any previously known strategies and detect more faults.

Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

Performance Improvement by Controlling Se/metal Ratio and Na₂S Post Deposition Treatment in Cu(In,Ga)₃Se<SUB>5</SUB> Thin-Film Solar cell

Hui-Ling Cui,Seung Tae Kim,R.B.V. Chalapathy,Ji Hye Kim,Byung Tae Ahn 한국태양광발전학회 2019 Current Photovoltaic Research Vol.7 No.4

Cu(In,Ga)₃Se5 (β-CIGS) has a band gap of 1.35 eV, which is an optimum value for high solar-energy conversion efficiency. The effects of Cu and Ga content on the cell performance were investigated previously. However, the effect of Se content on the cell performance is not well understood yet. In this work, β-CIGS films were fabricated by three-stage co-evaporation of elemental sources with various Se fluxes at the third stage instead of at all stages. The average composition of five samples was Cu1.05(In0.59,Ga0.41)₃Sey, where the stoichiometric y value is 5.03 and the stoichiometric Se/metal (Se/M) ratio is 1.24. We varied the Se/metal ratio in a range from 1.18 to 1.28. We found that the best efficiency was achieved when the Se/M ratio was 1.24, which is exactly the stoichiometric value where the CIGS grains on the CIGS surface were tightly connected and faceted. With the optimum Se/M ratio, we were able to enhance the cell efficiency of a β-CIGS solar cell from 9.6% to 12.0% by employing a Na2S post deposition treatment. Our results indicate that Na₂S post deposition treatment is very effective to enhance the cell efficiency to a level on par with that in α-CIGS cell.

Using a Functional Epoxy, Micron Silver Flakes, Nano Silver Spheres, and Treated Single-Wall Carbon Nanotubes to Prepare High Performance Electrically Conductive Adhesives

Hui-wang Cui,Dong-sheng Li,Qiong Fan 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.3

In this study, a matrix resin containing a functional epoxy, a reactive diluent, a silane-coupling agent, and a curing agent was used to fabricate three modal electrically conductive adhesives (ECAs) with micron silver flakes, nano silver spheres, and treated single-wall carbon nanotubes (CNT). Results showed that too many micron silver flakes reduced the bulk resistivity and adhesion strength of uni-modal ECAs (matrix resin and micron silver flakes). As the nano silver spheres increased, the bulk resistivity of bi-modal ECAs (matrix resin, micron silver flakes, and nano silver spheres) firstly decreased, and then increased again. The adhesion strength decreased also. The bulk resistivity and adhesion strength of tri-modal ECAs (matrix resin, micron silver flakes, nano silver spheres, and treated CNT) both were reduced by the treated CNT greatly. These ECAs could be cured at 120°C or any higher temperature than this with different curing time. They all had high temperature stability with a pyrolysis temperature above 350°C and a glass transition temperature around 180°C.

Using Nano Hexagonal Boron Nitride Particles and Nano Cubic Silicon Carbide Particles to Improve the Thermal Conductivity of Electrically Conductive Adhesives

Hui-wang Cui,Dong-sheng Li,Qiong Fan 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.1

To satisfy the high electrical and thermal conductivity required for the continuous development of electronic products, nano hexagonal boron nitride (BN) particles and nano cubic silicon carbide (SiC) particles were added into electrically conductive adhesives (ECAs) to improve the thermal conductivity. BN and SiC had little negative effect on the electrical conductivity, but improved the thermal conductivity significantly. When their content was 1.5 wt. %, the thermal conductivity at 100°C, 150°C and 200°C was increased by 71%(100°C), 78% (150°C) and 70% (200°C), and 114% (100°C), 110% (150°C) and 98% (200°C) respectively for BN and SiC comparing with those of the ECAs with no thermal conductive fillers. This method is simple,easy to do, and can be used practically in electronic packaging.

Using Polyurethane, Ethylene-Vinyl Acetate Hotmelt, and Nano Hexagonal Boron Nitride Particles to Electrospin High Surface Adhesion Polymer Fibers

Hui-wang Cui,Xin Tang 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.1

In this study, we presented the use of polyurethane, ethylene-vinyl acetate hotmelt, and nano hexagonal boron nitride particles to prepare high surface adhesion polymer fibers via an elertrospinning method. The shear strength, dynamic tensile properties, and surface morphology have been investigated. These polymer fibers were found to have high shear strength, high tensile stress, and high tensile strain, which may have a good potential application in the matrix materials for thermal interface materials. Polymer fibers with and without nano hexagonal boron nitride particles showed the shear strength of 6.52 MPa and 5.44 MPa respectively on being heated up at 150°C for 45 min.

Extracorporeal Shock Wave Therapy Alters the Expression of Fibrosis-Related Molecules in Fibroblast Derived from Human Hypertrophic Scar

Cui, Hui Song,Hong, A Ram,Kim, June-Bum,Yu, Joo Hyang,Cho, Yoon Soo,Joo, So Young,Seo, Cheong Hoon MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.1

<P>Extracorporeal shock wave therapy (ESWT) considerably improves the appearance and symptoms of post-burn hypertrophic scars (HTS). However, the mechanism underlying the observed beneficial effects is not well understood. The objective of this study was to elucidate the mechanism underlying changes in cellular and molecular biology that is induced by ESWT of fibroblasts derived from scar tissue (HTSFs). We cultured primary dermal fibroblasts derived from human HTS and exposed these cells to 1000 impulses of 0.03, 0.1, and 0.3 mJ/mm<SUP>2</SUP>. At 24 h and 72 h after treatment, real-time PCR and western blotting were used to detect mRNA and protein expression, respectively, and cell viability and mobility were assessed. While HTSF viability was not affected, migration was decreased by ESWT. Transforming growth factor beta 1 (TGF-β1) expression was reduced and alpha smooth muscle actin (α-SMA), collagen-I, fibronectin, and twist-1 were reduced significantly after ESWT. Expression of E-cadherin was increased, while that of N-cadherin was reduced. Expression of inhibitor of DNA binding 1 and 2 was increased. In conclusion, suppressed epithelial-mesenchymal transition might be responsible for the anti-scarring effect of ESWT, and has potential as a therapeutic target in the management of post-burn scars.</P>

3차원 웨이블릿 기반 영상 압축 기술에 관한 연구

CUI HUI ZI,문주희(Joo-hee Moon) 대한전자공학회 2007 대한전자공학회 학술대회 Vol.2007 No.7

In previous work, each codeblock is coded using a context which is adaptively selected from three kinds of scanning directions after 3D DWT. But some subbands still have correlations among the coefficients in horizontal, vertical or temporal direction. In this paper, we propose a new 3D DWT-based video compression technique in which the difference of coefficients is calculated in one of three directions for every codeblocks and coded by a context adaptively selected for each codeblock. Experimental results show that the proposed compression technique outperforms measurably and visually compared to the conventional DWT-based techniques.

Relative Risk of Metabolic Syndrome in Middle Aged Adults with Different Weight Living in Urban Beijing, China

Cui Zhao-Hui,Li Yan-Ping,Liu Ai-Ling,Zhang Qian,Du Wei-Jing,Ma Guan-Sheng The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

The purpose of this study is to compare the relative risk of metabolic syndrome (MS) in middle aged adults with different body weights. 155 subjects living in urban Beijing were recruited from 24 neighborhood committees of urban Beijing. They were divided into normal weight, overweight and obese groups according to their BMIs. The general information of the subjects was collected using an interview-administered questionnaire. Standard procedure was followed to measure subject's weight, height and waist. Biochemical parameters (total cholesterol (TC), low- and high­density lipoprotein cholesterol (LDL-C ; HDL-C), triglyceride (TG), and fasting glucose) and blood pressure were also determined. The results indicated that the systolic and diastolic blood pressure, HDL-C of obese group was lower than that of the normal weight group. Fasting glucose of obese males was significantly higher than that of normal weight males. No significant difference of fasting glucose was found among female groups. No significant difference of TG was found among male groups, while TG of overweight and obese females was both significantly higher than normal weight females. There was no significant difference of TC and LDL-C among normal weight, overweight and obese groups in both males and females. The MS rate of obese males was significantly higher than the normal weight and overweight males, as was the female. The relative risk of MS in obese group was about 11 times higher (OR=11.249, $95\%CI$ = 3.812 - 33.191) than the normal weight group after adjusting for age, gender, smoking, drinking, family economic level and education status. It is concluded that obesity contributed to lower HDL-C, hypertriglyceride, hypertension and MS after controlling the effects of age, gender, socioeconomic status, alcohol drinking and smoking. Obese individuals have a higher risk of having MS than their normal weight counterparts.