Configuration Design and Optimization of a Novel Two-Mode Compound Power-Split Hybrid System

Hu Minghui,Zeng Li,Fu Guangshun,Zhou Anjian,Li Zhonghua,Qin Datong 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.4

Through the analysis of a CHS (Chinese hybrid system) compound power-split configuration, it is found that enhancing the system's speed decoupling ability can reduce the electric power loss, optimize the engine operating point distribution, and enhance the vehicle dynamics. This paper proposes a novel compound power-split configuration with a twospeed AMT (Automated manual transmission), which includes an electric-vehicle mode, a low-speed power-split mode, and a high-speed power-split mode. First, the electric power characteristics and rotational speed/torque characteristics of the CHS compound power-split configuration are analyzed using the lever method, theoretically demonstrating the rationality of the two-speed AMT+ compound power-split configuration. Then, the genetic algorithm is employed to optimize the multiparameters and multi-objectives in terms of the vehicle dynamics and economy. Finally, a comparative simulation and analysis of the vehicle’s economic and dynamic properties is performed for the new two-speed AMT+ compound power-split configuration and the original CHS compound power-split configuration. The results show that the vehicle economy is increased by 6.9 % for the new configuration, the acceleration time per 100 kilometers is decreased by 27.5 %, and the maximum speed is increased by 13.2 %.

From Fragmented Development to Three-Dimensional and Coordinated Development - Research on Renewal Strategies of Existing Underground Commercial Space in Harbin

Xue, Minghui,Su, Yiming,Hu, Jiayu Council on Tall Building and Urban Habitat Korea 2021 International journal of high-rise buildings Vol.10 No.1

In Harbin, a network of underground commercial spaces has been developed to occupy spaces that were originally created as civil defense shelters. With the gradual extension of the local metro rail system, the existing underground commercial space is no longer an isolated regional development, but a space that represents "three-dimensional city" and coordinated development taking place in many Chinese cities. Based on the analysis of the unique development process taken in underground space of Harbin, this paper summarizes three characteristics of its early model of "fragmented development" of underground space. By conducting a comprehensive field research and survey, the researchers analyzed 472 questionnaires related to the development trend, and proposed multi-level synergistic elements for the renewal and development of underground commercial space. The paper concludes by discussing the trend of "three-dimensional and collaborative development," suitable for the development needs of the new era, and the corresponding development strategies for the renewal of underground space.

Expression profiles of genes involved in fatty acid and lipid biosynthesis in developing seeds of Paeonia ostii

Li Chaoqiong,Hu Lizong,Que Beibei,Hu Yueran,Guo Yuanyuan,Zhang Minghui,Wang Zenan,Wang Xueqin,Liu Hongzhan,Wang Junsheng,Tian Huihui,Li Xiaoli 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background Paeonia ostii seeds were identifed as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefts. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds. Objectives This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development. Methods Preliminary research on Paeonia ostii seed development was carried out from 10 days after pollination until maturity, focusing on phenology, oil content and lipid profles. In addition, we investigated the spatiotemporal expression of 36 lipid biosynthetic genes in Paeonia ostii by using quantitative real-time PCR. Results The results suggested that the development of Paeonia ostii seeds from pollination to maturity could be divided into three periods. The 36 lipid genes showed various spatiotemporal expression patterns and fve gene groups with distinct temporal patterns during seed development were identifed by clustering analysis of expression data. Furthermore, the relationships between gene expression and lipid/fatty acid accumulation and some candidate key lipid genes were discussed. Conclusions This study provided the global patterns of fatty acid and lipid biosynthesis-related gene expression, which are critical to understanding the molecular basis of lipid biosynthesis and identifying the lipid accumulation rate-limiting genes during seed development.

A tuning scheme of cycle reference point for gas turbine adaptive performance simulation with field data

Binbin Yan,Minghui Hu,Kun Feng,Zhinong Jiang 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.12

To minimize the simulated performance error of gas turbines, traditional adaptive methods are mainly concerned with the tuning of cycle design point and component maps given by the manufacturer, usually ignoring the fact that performance at cycle design point may not match the field data due to the deviation between test-rig conditions and field conditions. In this paper, a new tuning scheme of the cycle reference point is proposed to minimize the simulated errors simultaneously at design point and off-design points. The scheme is composed of a backward iteration algorithm and a genetic algorithm. During the backward iteration, the field data at the maximum operating condition is selected to obtain the initial cycle reference point with several undetermined parameters. Further, the genetic algorithm is used to optimize the undetermined parameters. The accuracy of the proposed method was validated by the simulated performance of a PGT25+ gas turbine.

Deficiency of optineurin enhances osteoclast differentiation by attenuating the NRF2-mediated antioxidant response

Xue Peng,Hu Xiangxiang,Chang Emily,Wang Lufei,Chen Minghui,Wu Tai-Hsien,Lee Dong-Joon,Foster Brian L.,Tseng Henry C.,Ko Ching-Chang 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Abnormally increased resorption contributes to bone degenerative diseases such as Paget’s disease of bone (PDB) through unclear mechanisms. Recently, the optineurin (OPTN) gene has been implicated in PDB, and global OPTN knockout mice ( Optn −/− ) were shown to exhibit increased formation of osteoclasts (osteoclastogenesis). Growing evidence, including our own, has demonstrated that intracellular reactive oxygen species (ROS) stimulated by receptor activator of nuclear factor kappa-B ligand (RANKL) can act as signaling molecules to promote osteoclastogenesis. Here, we report that OPTN interacts with nuclear factor erythroid-derived factor 2-related factor 2 (NRF2), the master regulator of the antioxidant response, defining a pathway through which RANKL-induced ROS could be regulated for osteoclastogenesis. In this study, monocytes from Optn −/− and wild-type ( Optn +/+ ) mice were utilized to differentiate into osteoclasts, and both qRT-PCR and tartrate-resistant acid phosphatase (TRAP) staining showed that the Optn −/− monocytes exhibited enhanced osteoclastogenesis compared to the Optn +/+ cells. CellROX ® staining, qRT-PCR, and Western blotting indicated that OPTN deficiency reduced the basal expression of Nrf2 , inhibited the expression of NRF2-responsive antioxidants, and increased basal and RANKL-induced intracellular ROS levels, leading to enhanced osteoclastogenesis. Coimmunoprecipitation (co-IP) showed direct interaction, and immunofluorescence staining showed perinuclear colocalization of the OPTN-NRF2 granular structures during differentiation. Finally, curcumin and the other NRF2 activators attenuated the hyperactive osteoclastogenesis induced by OPTN deficiency. Collectively, our findings reveal a novel OPTN-mediated mechanism for regulating the NRF2-mediated antioxidant response in osteoclasts and extend the therapeutic potential of OPTN in the aging process resulting from ROS-triggered oxidative stress, which is associated with PDB and many other degenerative diseases.

The Novel Pathogenic Mutation c.849dupT in BRCA2 Contributes to the Nonsense-Mediated mRNA Decay of BRCA2 in Familial Breast Cancer

Sanrong Li,Jing Ma,Caiying Hu,Xing Zhang,Deyong Xiao,Lili Hao,Wenjun Xiao,Jichun Yang,Ling Hu,Xiaowei Liu,Minghui Dong,Duan Ma,Rensheng Liu 한국유방암학회 2018 Journal of breast cancer Vol.21 No.3

In this study, we used next-generation sequencing methods to screen 300 individuals for BRCA1 and BRCA2. A novel mutation (c.849dupT) in BRCA2 was identified in a female patient and her unaffected brothers. This mutation leads to the truncation of BRCA2 functional domains. Moreover, BRCA2 mRNA expression levels in mutation carriers are significantly reduced compared to noncarriers. Immunofluorescence and western blot assays showed that this mutation resulted in reduced BRCA2 protein expression. Thus, we identified a novel mutation that damaged the function and expression of BRCA2 in a family with breast cancer history. The pedigree analysis suggested that this mutation is strongly associated with familial breast cancer. Genetic counsellors suggest that mutation carriers in this family undergo routine screening for breast cancer, as well as other malignancies, such as prostate and ovarian cancer. The effects of this BRCA2 mutation on drug resistance should be taken into consideration during treatment.

Effect of specific functional groups on oil adhesion from mica substrate: Implications for low salinity effect

Jiazhong Wu,Fanghui Liu,Hui Yang,Shijing Xu,Quan Xie,Minghui Zhang,TING CHEN,Guangxin Hu,Jinben Wang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-

Low salinity effect has been in the center of attention as a cost-effective and environmentally friendlytechnique. Wettability alteration of the oil/brine/mica system appears to be the identified mechanism(s)to trigger the low salinity effect. While the effect of water chemistry and minerology on the wettability ofthe system has been extensively investigated, few studies have investigated the effect of specific functiongroups from crude oil on the system wettability, limiting the understanding of how specific functionalgroup contributes to the wettability. We thus experimentally measured the adhesion forces betweenmica surfaces and functional groups (e.g., C6H5–, CH3–, COOH–, and NH2–) in the presence of differentaqueous ionic solutions using chemical force microscopy (CFM). Moreover, to understand thecontribution of the structural force, the traditional Derjaguin–Landau–Verwey–Overbeek (DLVO) theorywas extended (denoted as EDLVO) tofit the force profiles using a Gauss model. Our results showed thatthe adhesion force between mica and functional groups in a decreasing order was –NH2> –COOH> –CH3> –C6H5. We also found that while DLVO forces strongly affected the tip-surface contact due to theinteractions among oil/brine/mica interfaces, the structural forces also played an important role in adistance of 1–20 nm due to the presence of H-bonds between COOH-terminated or NH2-terminated tipand mica surface. We therefore conclude that the structural force largely contributes to the adhesionforce due to the hydrophilicity or polarity of functional groups, and nucleophilic property (such as phenylgroup). Our results suggest that the polarity of the crude oil needs to be considered to screen a candidatereservoir for low salinity waterflooding projects.