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나춘기,이무성,홍순강 木浦大學校 工業技術硏究所 1997 工業技術硏究誌 Vol.7 No.-
The purpose of this study is to examine the reusability of fly ashs produced by coal-fired power plants as an raw material in the manufacturing process of building resources such as brick, by investigating, in first, the mineralogical and chemical properties of raw materials including fly ashs of the Honam and the Samcheonpo thermal power plants and clay of the Hwangdeung area, and then examining the quility of pilot productions of fly ash-clay brick having various mixing proportions of fly ash. In general, the coal fly ashs separated in grain size under 200mesh and of Honam power plant have higher alkali contents but lower unburned carbon residues than those over 200mesh and of Samcheonpo power plant. This higher contents of alkali components such as CaO and Na2O and lower contents of unburned carbon have contributed to make dense structure in fly ahs-clay brick manufactured. Therefore, the clay brick manufactured by mixture of the Honam fly ash have more dence structure and much higher compressional strength than that of the Samcheonpo, and shows the trend of increasing the compressional strength from 1100-1300kg/㎠ to 170kg/㎠ with increasing the proportion of fly ash part from 10% to 50%. In contrast to Honam fly ash, the compressional strength of clay brick produced by the Samcheonpo fly ash decrease from 600-720kg/㎠ to 270-300kg/㎠ with increasing the mixing ratio of fly ash from 10% to 50%, due to high unburned carbon contents and low alkali contents of Samcheonpo fly ash.
Transcriptome sequencing and analysis of sweet osmanthus (Osmanthus fragrans Lour.)
Hong Na Mu,Liang Gui Wang,Huo Gen Li,Xiu Lian Yang,Tao Ze Sun,Chen Xu 한국유전학회 2014 Genes & Genomics Vol.36 No.6
Osmanthus fragrans is a woody, evergreenspecies of shrubs and small trees that is extensively plantedin sub-tropical and temperate climates as an ornamentalplant in gardens and for its health benefits. The flower colorranges from ivory to orange to pink among different varietiesand even color difference during the whole blossom inthe sweet osmanthus. Sweet osmanthus is widely cultivatedthroughout China and other countries due to its prominentfragrance, colorful flowers, and medicinal properties. However, the scanty genomic resources in the Olea familyhave greatly hindered further exploration of its geneticmechanism on these economically important traits. In thisstudy, transcriptome sequencing of O. fragrans was performedusing the Illumina HighSeqTM2000 sequencingplatform. Next generation sequencing (NGS) of the transcriptomeof O. fragrans produced 31.7G of clean bases(211,266,818 clean reads) that were assembled into256,774 transcripts and 117,595 unigenes. Of them, 197and 237 candidate genes involved in fragrance and pigmentbiosynthesis respectively were identified based on functionannotation. Meanwhile, 1 unnamed protein and 468 functionalunknown genes were also identified. Furthermore,mRNA sequencing expression profiling of O. fragranswere compared to previous genes’. In summary, thiscomprehensive transcriptome dataset allows the identificationof genes associated with several major metabolicpathways and provides a useful public information platformfor further functional genomic studiesin O. fragransLour.
牡丹皮의 PC12 cell 항산화 효과와 관련 HO, MIF, COMT 유전자 발현에 미치는 영향
손무성,노삼웅,고은정,나영인,조종운,배현수,홍무창,신민규 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4
Oriental medicine explains aging as the weakening of Kidney-Qi, and Kidney-strengthening herbal medicines such as Yukmijihwang-tang have been studied for anti-aging effects. In Western Medicine, the hypothesis that reactive oxidant species(ROS) contribute to the aging process is generally accepted. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective constituent of Yukmijihwang-tang in decreasing ROS production in oxidative-stressed cells. The purpose of this study is to confirm the anti-oxidant effect of MCR on PC12 cells, the expression of Heme oxygenase (HO), Macrophage migration inhibitory factor (MIF), Catechol-0-methyltransferase (COMT) using real time RT PCR. PC12 cells were treated without or with hydrogen peroxide in the presence or absence of MCR using MTS assay. Hydrogen peroxide decreased the viability of PC12 cells by 53% and MCR did not influence that of stressed PC 12 cells irrespective of dose or incubation period. However, MCR showed an inhibitory effect on production of ROS in stressed cells, both dose and incubation time dependently. In particular, 1 mg/ml of MCR for 24 h culture almost returned to normal level. In the quantiation of anti-aging related gene expression, MCR at 1 mg/ml increased the expression of HO by 370%, MlF by 180% and COMT by 280% through real time RT PCR. In conclusion, MCR treatment protected PC12 cells from hydrogen peroxide and decreased ROS production and enhanced anti-oxidative gene expression such as HO, COMT and MlF, which suggests that MCR is involved in controlling anti-aging of nerve cells through elimination of cytotoxic stimuli..
천왕보심단(天王補心丹)∘쌀발효추출물(GABAː gamma-aminobutyric acid 30%) 복합물의 抗스트레스에 대한 유효성 연구ː무작위 배정 및 이중맹검
홍초영 ( Choyoung Hong ),서상권 ( Seo Sang Gwon ),나승곤 ( Seunggon Na ),조호성 ( Hosong Cho ),이미경 ( Mi-kyung Lee ),진무현 ( Mu Hyun Jin ),노석선 ( Seok-seon Roh ),주영승 ( Youngsung Ju ) 대한본초학회 2023 大韓本草學會誌 Vol.38 No.2
Objectives : From this study, we sight to identify Anti-stress effect of Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex Methods : In order to measure the degree of functional evaluation of tension relief due to stress, it was evaluated whether the psychological indicators VAS(Visual Analog Scale) and POMS(Profile Of Mood State) were improved. Which is generally used for stress measurement and has a high frequency of clinical use. All clinical trial subjects were required to take the drug once a day at 9 ± 30 min in the morning for 2 weeks. Results : After intake, the group that Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex had a statistically significant improvement effect compared to before intake due to an improved 30.81% of the VAS scores. The POMS-T score improved 54.13%, the POMS-V score improved 117.5% and the POMS-F score improved 59%, which had a statistically significant effect compared to Placebo group (p<0.001). There is a significant difference between two group, so even considering the placebo effect, the anti stress effect was confirmed in the Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex intake group. Conclusions : The above results showed that the Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA ː gamma-aminobutyric acid 30%) Complex had an anti-stress effect. Therefore, it is expected to be developed as a safe and comfortable mental care health functional food that can reduce the risk of drug abuse.
Pseudoalteromonas sp. 배양액으로부터 아가레이즈 분리를 위한 음이온교환 크로마토그래피 최적화
김유나 ( Yu Na Kim ),이재란 ( Jae Ran Lee ),김무찬 ( Mu Chan Kim ),김성배 ( Sung Bae Kim ),장용근 ( Yong Keun Chang ),홍순광 ( Soon Kwang Hong ),김창준 ( Chang Joon Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.6
아가로오스 분해산물은 생리활성이 우수하여 의약품 및 기능성 화장품 원료로 사용될 뿐만 아니라 바이오에탄올 생산을 위한 효모 발효용 기질로 검토되고 있어 상당한 주목을 받고 있다. 이에 따라 고성능 아가레이즈 탐색에 대한 연구가 활발히 진행되고 있다. 최근 본 연구팀에서는 남해안에서 신규 아가레이즈를 생산하는 미생물인 Pseudoalteromonas sp.를 분리하였다. 본 연구에서는 음이온교환수지를 이용하여 미생물 배양액으로부터 아가레이즈를 분리·정제하기 위한 크로마토그래피 조건을 최적화 하였다. 황산암모늄을 첨가하여 배양 상등액으로부터 침전된 단백질을 회수하고 이를 투석하여 조효소액을 얻었다. 음이온 교환수지인 DEAE-Sepharose 레진이 충진된 칼럼에 조효소액을 로딩하여 아가레이즈를 분리하였다. 410 μg의 단백질 로딩 시 흡착에 적합한 평형 pH는 7.5~8.0 이었고, 적정한 resin의 부피는 3 mL였다. 등용매 용출에서 용출액 중의 NaCl 농도가 증가함에 따라 용출되는 단백질 양이 증가하여 400 mM의 NaCl에서 최대에 달하였다. 최종적으로, NaCl 농도를 1,000 mM까지 선형적으로 증가시키며 아가레이즈를 분리하였다. Lugol 용액을 이용한 염색법으로 용리액 중의 아가레이즈의 존재를 확인하였다. Degradation products of agarose are biologically active and thus used as an ingredient in pharmaceuticals or functional cosmetics. Furthermore, it has been strongly considered as a substrate for bio-ethanol fermentation. Recently, we isolated new agarase-producing microorganism, Pseudoalteromonas sp. from south sea of Korea. In this study, we aimed to separate and purify the agarase from culture broth of this strain. Separation of agarase was performed by ion- exchange chromatography on DEAE-Sepharose resin. Equilibrium pH and volume ratio of resin to the amount of protein were optimized for the efficient adsorption of protein. 410 μg of protein was completely adsorbed to 3 mL of resin at pH 7.5. The total amount of eluted protein increased as NaCl concentration increased to 400 mM at iso-cratic elution. Agarase was separated by linear gradient elution of NaCl (0~1,000 mM). Three major protein peaks were observed and the presence or absence of agarase in these eluted proteins was measured by Lugol`s staining technique. Only six eluted protein fractions showed strong agarase activity.
p73 G4C14 to A4T14 polymorphism is associated with colorectal cancer risk and survival.
Lee, Kyung-Eun,Hong, Young-Seoub,Kim, Byoung-Gwon,Kim, Na-Young,Lee, Kyoung-Mu,Kwak, Jong-Young,Roh, Mee-Sook WJG Press 2010 WORLD JOURNAL OF GASTROENTEROLOGY Vol.16 No.35
<P>To analyze the association between the p73 G4C14-to-A4T14 polymorphism (a.k.a., the GC/AT variation) and colorectal cancer risk and survival in the Korean population, and to evaluate the relationships between p73 polymorphism and the p73 protein expression or clinicopathological characteristics of colorectal cancer.</P>
식도C형 간염 환자에서 원발성 항인지질 항체와 연관된 대장의 다발성 장간막혈전증과 문맥압 항진증 1예
오상우,최재홍,박선미,이지현,정상무,나병규,윤세진,박길선 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.6
The gastrointestinal rnanifestations associated with antiphospholipid antibodies include Budd-Chiari syndroame, hepatic infarction, portal hypertension, pancreatitis, intestinal infarc- tion, perforation, bleeding and ulceration. A 40-year old man, without prior thrombotic event, presented with severe abdominal pain for 3 days and septic shock. Multiple mesenteric venous thrombosis and colonic congestion were suggested by abdominal CT and angiography. Gastroendoscopy revealed esophageal varix and congestive gastropathy. Laboratory tests disclosed postive antiphospholipid antibodies, anti-HCV antibodies, HCV-PCR, prolonged PT, aPTT, thrombocytopenia and had no evidences of SLE and other connective tissue diseases. He was diagnosed as mutiple mesenteric thrombosis and portal hypertension associated with antiphospholipid antibodies in hepatitis C virus infection. He was improved with the antibiotics and intravenous vasopressors. He have had no other thrombotic events until one year after discharge.