WS-5 Extract of <i> Curcuma longa, Chaenomeles sinensis,</i> and <i> Zingiber officinale</i> Contains Anti-AChE Compounds and Improves <i>β</i> -Amyloid-Induced Memory Impairment in Mice

Kim, Ju Eun,Shrestha, Abinash Chandra,Kim, Hyo Shin,Ham, Ha Neul,Kim, Jun Hyeong,Kim, Yeong Jee,Noh, Yun Jeong,Kim, Su Jin,Kim, Dae Keun,Jo, Hyung Kwon,Kim, Dae Sung,Moon, Kwang Hyun,Lee, Jeong Ho,Jeo Hindawi 2019 Evidence-based Complementary and Alternative Medic Vol.2019 No.-

<P>Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (A<I>β</I>) as senile plaques associated with reactive astrocytes and microglial activation in the brain. The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against A<I>β</I>-induced AD in mice and to identify the extract's active constituents. In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE). Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented A<I>β</I> oligomerization via inhibition of A<I>β</I><SUB>1-42</SUB> aggregation. Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS). Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells. WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-<I>α</I> and IL-6. The memory impairment in mice with A<I>β</I>-induced AD was studied using the Morris water maze and passive avoidance test. Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain. The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced A<I>β</I> plaque accumulation in A<I>β</I>-induced AD. HPLC analysis identified the extract's active compounds that exert anti-AChE activity. In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.</P>

[PB-0087] Efficiency Analysis of Agrobacterium Transformation of Korean Wheat Varieties By GUS System

Ha Neul Lee(Ha Neul Lee),Geon Hee Lee(Geon Hee Lee),Jae Yoon Kim(Jae Yoon Kim) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

편측 요통을 호소하는 환자에 있어서 척추 주위 근육의 지방량과 통증과의 관계

김하늘 ( Ha Neul Kim ),김경훈 ( Kyoung Hun Kim ),김주원 ( Joo Won Kim ),진은석 ( Eun Seok Jin ),하인혁 ( In Hyuk Ha ),고동현 ( Dong Hyun Koh ),홍순성 ( Soon Sung Hong ),권혁준 ( Hyeok Joon Kwon ) 한방재활의학과학회 2009 한방재활의학과학회지 Vol.19 No.1

목적: 요통은 임상에서 가장 흔하게 접할 수 있는 질환이며, 동시에 통증으로 인한 근무 결손도나 생활 불편감이 높은 질병이다. 그래서, 많은 연구들이 요통을 일으키는 원인 혹은 악화시키는 유발 인자를 밝혀내려는데 초점이 맞추어져 있다. 이번 연구의 목적은 편측 요통을 호소하는 환자에 있어서 MRI에서 조사된 척추 주위 근육의 지방량과 통증과의 관계에 대해서 알아보는데 있다. 방법: 2007년 11월부터 2008년 4월까지 본원에 요통으로 내원한 환자를 대상으로, 편측 요통을 호소하며, 체질량 지수가 25kg/㎡ 이하이며, 만 20세이상 40세미만인 환자 24명을 대상으로 연구를 진행하였다. MRI에서 조사되어진 L4-5 디스크 레벨의 척추 주위 근육의 지방량을 객관적인 pseudocoloring technique을 이용해 측정하고, paired t-test로 결과를 분석하여 요통이 유발된 측과 지방량의 관계에 대해서 연구하였다. 결과: 요통이 있는 측의 지방량과 요통이 없는 측의 지방량을 측정하여 데이터를 분석하였다. 양측의 지방량과 통증의 유무에 대한 관계는 통계적으로 유의한 수치를 나타냈다(P <0.05). 요통이 있는 측과 없는 측과의 성별, 나이, BMI의 통계적인 차이는 없었으며, 지방량 측정시 남자들이 여자들에 비해 지방량이 더 높게 측정되었다(남자 8.5±5.1%, 여자 6.5±3.6%). 결론: 이 연구를 통해 척추 주위 근육의 지방량과 요통에 대한 상관성의 증거를 제시하였다. 잘 선별되어진 환자의 MRI를 통해 객관적인 방법으로 지방량을 체크를 하였으며, 추후 더 많은 개체수를 이용해 보다 더 정확한 관련성을 파악하고, 나아가 지방량에 따른 통증 강도의 상관성에 대해서 연구가 필요하리라 생각된다. Objectives: Low back pain(LBP) is a common disabling disease in clinical practice and loss of working hours due to this condition is huge. The aim of this study was to determine if there was an association between fat deposit of paraspinal muscles as observed on MRI scans in patients presenting with unilateral LBP. Methods: 24 patients who visiting our hospital with a clinical presentation of unilateral LBP were recruited to the study. Patients were between 20 and 30 years and had a history of unilateral LBP within 12 months. After MRI scaning, the images were saved in DICOM file format for Picture Archiving and Communication System(PACS). The percentage of fat infiltrated area was measured using a pseudocoloring technique. Data were analyzed comparing the fat deposits of the muscles on the symptomatic and asymptomatic sides. Paired t-test was used to find the difference between the measurements of fat tissue in individual patients. Results: The amount of fat in the symptomatic side was 7.6±4.51%, asymptomatic side was 6.7±4.29%. There were increases, statistically significant, in the fat changes of the paraspinal muscles at the L4-5 disc level(P <0.05). Also, men were likely than women to have more fat deposit in symptomatic side(men 8.5±5.1%, women 6.5±3.6%). Conclusions: The amount of fat in the symptomatic side shows significantly increased than asymptomatic side in the paraspinal muscles at the L4-5 disc level. It suggested that fat infiltration in the muscles associated with LBP. Further studies will be needed to confirm the relationship between the muscle fatty changes and LBP in the large sample size. In addition, the correlation of pain severity with fat infiltration needs to be addressed.

Changes of Physical Properties by Different Parts and Heat Treatments of Lentinula edodes

Neul-i Ha,Seung-Bin Im,Seong-Woo Jin,Kyung-Je Kim,Young-Woo Koh,Hee-Kyung Jeong,Seung-Ju Kim,Kyong-Won Yun,Ki-Man Kim,Yu-Jin Choi,Da-Hye Song,Kyoung Sun Seo 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Crystal structure and functional characterization of SF216 from <i>Shigella flexneri</i>

Kim, Ha-Neul,Seok, Seung-Hyeon,Lee, Yoo-Sup,Won, Hyung-Sik,Seo, Min-Duk Wiley (John WileySons) 2017 FEBS letters Vol.591 No.21

<P>Shigella flexneri is a Gram-negative anaerobic bacterium that causes highly infectious bacterial dysentery in humans. Here, we solved the crystal structure of SF216, a hypothetical protein from the S.flexneri 5a strain M90T, at 1.7 angstrom resolution. The crystal structure of SF216 represents a homotrimer stabilized by intersubunit interactions and ion-mediated electrostatic interactions. Each subunit consists of three beta-strands and five alpha-helices with the beta-beta-beta-alpha-alpha-alpha-alpha-alpha topology. Based on the structural information, we also demonstrate that SF216 shows weak ribonuclease activity by a fluorescence quenching assay. Furthermore, we identify potential druggable pockets (putative hot spots) on the surface of the SF216 structure by computational mapping.</P>

CT Findings of Azygos Venous System: Congenital Variants and Acquired Structural Changes

Ha Neul Kim,Youkyung Lee,Su Jin Hong,Ji Hun Kang,Ji-hoon Jung 대한영상의학회 2024 대한영상의학회지 Vol.85 No.1

The azygos venous system is a crucial conduit of the posterior thorax and potentially vital collateral pathway. However, it is often overlooked clinically and radiologically. This pictorial essay reviews the normal azygos venous anatomy and CT findings of congenital variations and structural changes associated with acquired pathologies.

The catalytic role of additive components for the nitridation of silicon/additive mixture

KIM, Ha-Neul,PARK, Young-Jo,KIM, Jin-Myung,LEE, Jae-Wook,KO, Jae-Woong,KIM, Hai-Doo,LEE, Seung-Jun,BAEK, Seung-Su,SEO, Il-Sung Ceramic Society of Japan 2016 JOURNAL OF THE CERAMIC SOCIETY OF JAPAN - Vol.124 No.3

<P>The nitriding behavior of silicon/additives mixture with varied composition was investigated by focusing on the catalytic effect of sintering additives (Y2O3 and Al2O3) and diluents (Si3N4). While sintering additives have originally been mixed to form a liquid phase to promote both densification and grain growth during post-sintering, it was revealed that the addition of Y2O3 enhances the nitriding reaction by eliminating the SiO2 and Si3N4 layer on the surface of silicon particles. Further, a synergistic effect resulting in an increased nitridation was verified by concurrent addition of sintering additives and diluents. The contribution of each component of the compact to the promoted nitriding reaction was elucidated in quasi in-situ manner by the combination of phase analysis and thermogravimetric analysis. (C) 2016 The Ceramic Society of Japan. All rights reserved.</P>

Cordyceps militaris alleviates non-alcoholic fatty liver disease in ob/ob mice

Ha-Neul Choi,Yang-Hee Jang,Min-Joo Kim,Min Jeong Seo,Byoung Won Kang,Yong Kee Jeong3,,Jung-In Kim 대한지역사회영양학회 2014 Nutrition Research and Practice Vol.8 No.2

BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is becoming an important public health problem as metabolic syndrome and type 2 diabetes have become epidemic. In this study we investigated the protective effect of Cordyceps militaris (C. militaris) against NAFLD in an obese mouse model. MATERIALS/METHODS: Four-week-old male ob/ob mice were fed an AIN-93G diet or a diet containing 1% C. militaris water extract for 10 weeks after 1 week of adaptation. Serum glucose, insulin, free fatty acid (FFA), alanine transaminase (ALT), and proinflammatory cytokines were measured. Hepatic levels of lipids, glutathione (GSH), and lipid peroxide were determined. RESULTS: Consumption of C. militaris significantly decreased serum glucose, as well as homeostasis model assessment for insulin resistance (HOMA-IR), in ob/ob mice. In addition to lowering serum FFA levels, C. militaris also significantly decreased hepatic total lipids and triglyceride contents. Serum ALT activities and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were reduced by C. militaris. Consumption of C. militaris increased hepatic GSH and reduced lipid peroxide levels. CONCLUSIONS: These results indicate that C. militaris can exert protective effects against development of NAFLD, partly by reducing inflammatory cytokines and improving hepatic antioxidant status in ob/ob mice.

Sinterability of Low-Cost 3Y-ZrO₂ Powder and Mechanical Properties of the Sintered Body

Kim, Min-Sung,Go, Shin-Il,Kim, Jin-Myung,Park, Young-Jo,Kim, Ha-Neul,Ko, Jae-Woong,Jung, Seung-Hwa,Kim, Jae-Yuk,Yun, Jon-Do The Korean Ceramic Society 2017 한국세라믹학회지 Vol.54 No.4

This study investigated the effects of grain size and phase constitution on the mechanical properties of $3Y-ZrO_2$ by varying the sintering conditions. The raw powder prepared by a low-cost wet milling using the coarse solid oxide powders was sintered by both pressureless sintering and hot-pressing, respectively. As increasing holding time at $1450^{\circ}C$ for pressureless sintering, it promoted the microstructural coarsening of matrix grains and the phase transformation to tetragonal phase, whereas the bimodal microstructure embedded with abnormal $cubic-ZrO_2$ grains was observed regardless of sintering time. On the other hand, the specimens hot-pressed at $1300^{\circ}C$ for 2 h reached ~ 97% of relative density with homogeneous fine microstructure and mixed phase constitution. It was found that the proportion of untransformed monoclinic zirconia had the most adverse effect on the biaxial strength compared to the impacts of grain size and density. The pressureless sintering of the low-cost powder for prolonged sintering time to 8 h led to a decent combination of mechanical properties ($H_V=13.2GPa$, $K_{IC}=8.16MPa{\cdot}m^{1/2}$, ${\sigma}=981MPa$).

Recombinant Human Acidic Fibroblast Growth Factor (aFGF) Expressed in Nicotiana benthamiana Potentially Inhibits Skin Photoaging

Ha, Jang-Ho,Kim, Ha-Neul,Moon, Ki-Beom,Jeon, Jae-Heung,Jung, Dai-Hyun,Kim, Su-Jung,Mason, Hugh,Shin, Seo-Yeon,Kim, Hyun-Soon,Park, Kyung-Mok Georg Thieme Verlag KG 2017 Planta medica Vol.83 No.10

<B>Abstract</B><P>Responding to the need for recombinant acidic fibroblast growth factor in the pharmaceutical and cosmetic industries, we established a scalable expression system for recombinant human aFGF using transient and a DNA replicon vector expression in Nicotiana benthamiana. Recombinant human-acidic fibroblast growth factor was recovered following Agrobacterium infiltration of N. benthamiana. The optimal time point at which to harvest recombinant human acidic fibroblast growth factor expressing leaves was found to be 4 days post-infiltration, before necrosis was evident. Commassie-stained SDS-PAGE gels of His-tag column eluates, concentrated using a 10 000 molecular weight cut-off column, showed an intense band at the expected molecular weight for recombinant human acidic fibroblast growth factor. An immunoblot confirmed that this band was recombinant human acidic fibroblast growth factor. Up to 10 µg recombinant human-acidic fibroblast growth factor/g of fresh leaves were achieved by a simple affinity purification protocol using protein extract from the leaves of agroinfiltrated N. benthamiana. The purified recombinant human acidic fibroblast growth factor improved the survival rate of UVB-irradiated HaCaT and CCD-986sk cells approximately 89 and 81 %, respectively. N. benthamiana-derived recombinant human acidic fibroblast growth factor showed similar effects on skin cell proliferation and UVB protection compared to those of Escherichia coli-derived recombinant human acidic fibroblast growth factor. Additionally, N. benthamiana-derived recombinant human acidic fibroblast growth factor increased type 1 procollagen synthesis up to 30 % as well as reduced UVB-induced intracellular reactive oxygen species generation in fibroblast (CCD-986sk) cells.</P><P>UVB is a well-known factor that causes various types of skin damage and premature aging. Therefore, the present study demonstrated that N. benthamiana-derived recombinant human acidic fibroblast growth factor effectively protects skin cell from UVB, suggesting its potential use as a cosmetic or therapeutic agent against skin photoaging.</P>