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Gonhyung Kim , Ji-Hye Kim, Bibek Sutradhar, Seok Hwa Choi 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.2
Peripheral nerve injuries are very common in clinics and often result in severe functional deficits. The aim of this study was to evaluate the effect of treadmill running and electro-acupuncture on nerve regeneration and functional recovery of muscle activity following sciatic nerve crush injury in a rat model. A comparative study was conducted over 30 days on 60 adult male Sprague-Dawley rats grouped into sham control (C), electro-acupuncture (EA), treadmill (T), and treadmill plus electro-acupuncture (TEA). The left sciatic nerve was crushed for 30 sec using a hemostatic forceps and functional activity was evaluated with sciatic functional tests, nerve conduct velocity, muscle weight, and histology at 10, 20, and 30 days after injury. Muscle weight was significantly (P<0.05) increased between days 10 and 30 in the TEA group. In histology, the degree of damage was scored as C > TEA > T > EA, although necrosis and fibrosis of muscle was observed only in the TEA group. The EA and TEA groups showed rapid recovery with better myelinated axons on day 10. These results suggest that application of the TEA method with balanced exercise is a useful treatment option for peripheral nerve injury regeneration and muscle activity.
Male pseudohermaphroditism in a Maltese/poodle mixed dog
Seok-jin Jang, Young-jae Kim, Gonhyung Kim, Seok Hwa Choi 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.1
Male pseudohermaphroditism is not commonly reported in veterinary medicine. Here, a 3-year-old Maltese/poodle mixed dog presented with malformed external genitalia and episodic hematuria. Inspection and palpation of the external genitals showed a malformed penis, shortened prepuce, external urethral orifice, and cryptorchidism. There was no urethral meatus at the tip of the penis. The urethral opening was situated between the prepuce and the penis. The anterior half of the prepuce was absent, and the penis was free and exposed to both trauma and licking. Plain radiographic examination showed absence of an os penis in the penis. A double-contrast cystograph showed the suspected uterus as well as the cystic calculi. A hypoechoic space was seen at the dorsal portion of the urinary bladder. The space was suspected to be the uterus. A sagital ultrasonograph showed cystic calculi in the urinary bladder. During surgery to remove cystic calculi, hypoplastic testes as well as the uterus were observed. Histological examination of the testes showed the seminiferous tubules and interstitial cells. The sertoli cells and spermatogonia were adjacent to the basement membrane. No evidence of spermatogenesis was found. Striated squamous epithelial cells and smooth muscle cells were found in the uterus. This dog had vestigial oviducts as well as a uterus with male-appearing external genitals.
Effect of Feed Restriction in Modeling of Dietary Obesity
Yeoung Mi Cho,Sunhee Shin,Dongsun Park,Jeong Hee Jeon,Min-Jung Jang,Jwa Jin Kim,Jae Wook Kim,Hyeong-Jin Ji,Chang Hwan Kim,Seongjin Baek,Seok-Yeon Hwang,Gonhyung Kim,Yun-Bae Kim 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.4
Ad libitum feeding of normal diet to rats usually leads to overweight, showing higher body-mass index than 25, while the supplementation of high (20-40%) content of lard or beef tallow in the diet to make a high-fat diet (HFD)-induced obesity rather decreases body weights partially due to decrease in feed intake of HFD or occurrence of diarrhea. In our study, for modeling dietary obesity, rats were subjected to feed restriction by 20 and 40% with normal diet or HFD supplemented with 25% of lard for 6 weeks. Ad libitum (100%) feeding of HFD decreased body weight gain and body fat in rats, resulting from a lower feed intake and transient diarrhea, compared to that of animals fed normal diet. In comparison with ad libitum-feeding groups, HFD restricted to 60 and 80% resulted in higher body weight gain and body fat compared with the normal diet with corresponding restriction to 60% or 80%. The changes in body and fat weights exhibited a good relationship with blood lipids profiles and leptin level in feed restricted groups. In addition, body fat and lipid profiles were confirmed to be parallel with the lipid accumulation in the liver and blood parameters associated with liver function. Based on the results, it is suggested that an appropriate degree (80%) of dietary restriction could be a candidate of modeling HFD-induced obesity for the screening of antiobesity substances.
Kim, Jong Min,Son, Jun Sik,Kang, Seong Soo,Kim, Gonhyung,Choi, Seok Hwa Hindawi Publishing Corporation 2015 BioMed research international Vol.2015 No.-
<P>The aim of this study was to evaluate the bone regeneration of hydroxyapatite (HA)/alumina bilayered scaffold with a 3 mm passage-like medullary canal in a beagle tibia model. A porous HA/alumina scaffold was fabricated using a polymeric template-coating technique. HA/alumina scaffold dimensions were 10 mm in outer diameter, 20 mm in length, and with either a 3 mm passage or no passage. A 20 mm segmental defect was induced using an oscillating saw through the diaphysis of the beagle tibia. The defects of six beagles were filled with HA/alumina bilayered scaffolds with a 3 mm passage or without. The segmental defect was fixated using one bone plate and six screws. Bone regeneration within the HA/alumina scaffolds was observed at eight weeks after implantation. The evaluation of bone regeneration within the scaffolds after implantation in a beagle tibia was performed using radiography, computerized tomography (CT), micro-CT, and fluorescence microscopy. New bone successfully formed in the tibia defects treated with 3 mm passage HA/alumina scaffolds compared to without-passage HA/alumina scaffolds. It was concluded that the HA/alumina bilayered scaffold with 3 mm passage-like medullary canal was instrumental in inducing host-scaffold engraftment of the defect as well as distributing the newly formed bone throughout the scaffold at 8 weeks after implantation.</P>
Evaluation of Hypertriglyceridemia as a Mediator Between Endocrine Diseases and Pancreatitis in Dogs
Kim, Hakhyun,Kang, Ji-Houn,Heo, Tae-Young,Kang, Byeong-Teck,Kim, Gonhyung,Chang, Dongwoo,Na, Ki-Jeong,Yang, Mhan-Pyo American Animal Hospital Association 2019 The Journal of the American Animal Hospital Associ Vol.55 No.2
Kim, Jong Min,Han, Tae Sung,Park, Jinuk,Sutradhar, Bibek Chandra,Kim, Gonhyung,Kang, Seong Soo,Choi, Seok Hwa [s.n 2010 IN VIVO -ATHENS- Vol.24 No.6
<P>The rat model that is commonly used to study femoral head osteonecrosis involves the induction of osteonecrosis by surgery employing a lateral approach to the hip joint. However, this approach induces hip joint luxation, which means it is difficult to determine whether the osteonecrosis arises because of the vascular deprivation or because of the hip joint luxation. Whether or not femoral head osteonecrosis can be induced by the medial approach to the hip joint, which does not generate hip joint luxation, was assessed.</P>
A renal oncocytoma in a Maltese dog with renal cysts
Kim, Jong-uk,Park, Jinsung,Kim, Gonhyung,Chang, Dongwoo,Ahn, Byeongwoo The Korean Society of Veterinary Science 2019 大韓獸醫學會誌 Vol.59 No.1
A renal oncocytoma was diagnosed in an 8-year-old female Maltese dog with a history of renal cysts. Tumor cells were not detected until six months after observation of renal cysts. Nephrectomy was performed to treat the neoplasia. Tumor-like masses with numerous nodules were observed in the inner surface of cysts present in the caudal part of the left kidney. Histologically, the tumor consisted of cells with abundant eosinophilic cytoplasm. The diagnosis was based on histological features, periodic acid-Schiff reaction, and immunohistochemical cytokeratin staining. Based on a literature review, this is the first canine renal oncocytoma case reported in Korea.
Surgical Animal Models for Bone and Cartilage Regeneration
Gonhyung Kim(김근형) 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-
Tissue engineering for bone and cartilage which fail to treat effectively by severe damage and degeneration is still in the experimental stage. Stem cells, growth factors and scaffolds have been suggested as candidate therapeutic methods to develop neo-tissue during the last decade. A number of experimental surgical animal models are studied on different species of animals to treat the bone and cartilage defects. Rat, rabbit, dog, sheep and pig are frequently used for animal models. Partial or segmental defects are created in long bones such as femur, tibia, humerus, radius and ulna. However, orthopaedic experimental studies are more sensitive to infection and painful to the animals. It is therefore essential for accurate results to find suitable and successful surgical method in animal models.
Gonhyung Kim, Hye-Ran Gong, Seok Hwa Choi 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1
This study demonstrated that hyaluronic acid (HA) accelerated peripheral nerve regeneration after crush injury to the common peroneal nerve in an experimental rabbit model. Ten male New Zealand White rabbits, weighing 1.8 to 2.0 kg, were used in this study. After creating the nerve crush model in every right leg, rabbits were divided into two groups. Animals in group A received application of HA into the area surrounding the crushed nerve, and group B was the sham control. Electrophysiological assessment was performed every week. After 10 weeks, nerve histological examination, muscle weight and muscle histology were used to evaluate regeneration of the injured common peroneal nerve. No differences in electrophysiological assessment were observed between the two groups. In peripheral nerve histology, myelinated nerve fibers were observed more frequently and less connective tissue was observed in the crushed nerve of group A. Fewer muscle degenerative changes, such as fibrosis, atrophy, and centrally located myonuclei, were detected in group A than in group B. In conclusion, HA could become a potential neuroprotective agent for improvement of peripheral nerve regeneration after crush injury.