Transient propagation of BmLV and dysregulation of gene expression in nontarget cells following BmLV infection

Feng Yongjie,Zhang Xing,Kumar Dhiraj,Kuang Sulan,Liu Bo,Hu Xiaolong,Zhu Min,Liang Zi,Cao Guangli,Xue Renyu,Gong Chengliang 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Bombyx mori latent virus (BmLV), a novel positive-strand RNA virus was first identified in the B. mori cultured BmN cell line. Whether the infectivity of BmLV to silkworm larvae and non-silkworm cells is connected with dysregulation of gene expression are not well understood. A complete sequence of BmLV genomic RNA was identified and revealed that a fragment with 495 nt in length was deleted from the RNA-dependent RNA poly merase (RdRp) gene in some BmLV genomic RNAs. Studies on the infectivity of BmLV to nontarget cells showed that BmLV can infect silkworm larvae, Spodoptera frugiperda Sf-9 and H1299 lung cancer cells with transient propagation. The dysregulation of gene expression of Sf-9 cells followed by BmLV infection was analyzed. Out of 743 differentially expressed genes (DEGs), 300 were upregulated and 443 were downregulated. Gene Ontology (GO) analysis indicated the DEGs were enriched into oxidoreductase activity for CH-NH 2 group donors, gluta mate biosynthetic process, response to stress and proteasome core complex. KEGG enrichment analysis showed that DEGs were mainly enriched into sulfur metabolism, RNA degradation, proteasome, pentose and glucuronate interconversions. Undesirable nutrients and temperature factors contributed to the propagation of BmLV in Sf-9 cells. Additionally, the Imd and RNAi pathways were activated by BmLV infection without stimulating Toll and JAK-STAT pathways. Therefore, it is suggested that BmLV is originated from plants, which can enter nontarget cells with transient propagation. The transient infection of BmLV may not only be regulated by Imd and RNAi immune pathways but also mediated by dysregulation of gene expression.

Comparative transcriptome analysis of wing discs from Bombyx mori and Bombyx mandarina

Feng Yongjie,Kumar Dhiraj,Hu Xiaolong,Zhang Yiling,Zhu Min,Xue Renyu,Cao Guangli,Gong Chengliang 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

The insect wing is developed from the wing imaginal disc which is designed from the embryonic ectoderm. To get insight into gene expression profiles in wing discs of Bombyx mori during metamorphosis, we compared the gene expression in the wing between B. mori and B. mandarina moth through RNA-seq. Out of total valid reads identified from the 5th day of 5th instar larvae of silkworm (L5), 7th day of pupae (P7), 1st day of moth (M1) and 1st day of wild silkworm moth (WM1), 20,092,004, 29,251,647, 24,654,695 and 19,753,089 reads were mapped to the mRNA reference sequences of silkworm, respectively. 9229, 7048, 9268 and 6701 differentially expressed genes (DEGs) were respectively recorded in P7 vs L5, M1 vs P7, M1 vs L5 and WM1 vs M1. Further, the peroxisome, ribosome, endocytosis and oxidative phosphorylation pathways were significantly regulated in the metamorphosis of the silkworm. Our study identified 16 orthologous genes with a positive selection from M1, which might be subjected to artificial selection in the domestication of B. mori and would play vital roles in the flight of B. mandarina.

Cell proliferation can be modulated by receptor tyrosine kinase-like orphan receptor 2 in the silkworm, Bombyx mori

Yongjie Feng,Wei Liu,Dhiraj Kumar,Min Zhu,Renyu Xue,Guangli Cao,Xiaolong Hu,Chengliang Gong 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

The receptor tyrosine kinase-like orphan receptor 2 (Ror2) is involved in the Wnt/β-catenin signaling pathway which regulates cell proliferation, polarity, differentiation, migration, metabolism and survival. However, the function of Ror2 in the silkworm Bombyx mori is still undisclosed. In the present investigation, we have made an effort to clone the silkworm Ror2 gene (BmRor2). The sequence analysis showed that the open reading frame (ORF) was 1914 bp in size and encoded a protein with the conserved domains of Ror2 protein. The qRT-PCR results indicated that the BmRor2 gene expression level was the highest in the head among all identified tis sues on 3rd day of the fifth instar larvae. In the gonads of the different development stages, the BmRor2 gene expression level was highest on the 4th day of the fourth instar larvae. The immunofluorescence assay indicated that the BmRor2 protein was located at the cytomembrane. The effects of BmRor2 protein on the expression levels of genes related to TGF-β, Hippo, JAK-STAT and Notch signaling pathways were investigated by qRT-PCR. The expression levels of crumbs (crb), warts (wts), α-catenin (cat), four-jointed (fj), decapentaplegic (dpp), kibra ortholog (kibra), serrate (serr) and c-myc (myc) genes were down-regulated, whereas, suppressor of cytokine signaling 2 (socs 2) gene expression was up-regulated in the cultured BmN cells after the BmRor2 expression level was up-regulated. Further, cell proliferation was demoted and the size of cells was decreased when BmRor2 expression level was elevated. Our current finding recommended that BmRo2 can regulate TGF- β, Hippo, JAKSTAT, and Notch signaling pathways, and affect cell proliferation and size.

Separation of valuable metals from mixed cathode materials of spent lithium-ion batteries by single-stage extraction

Yongjie Li,Qiang Fu,Hongyun Qin,Kun Yang,Junnan Lv,Qicheng Zhang,Zhang Hui,Feng Liu,Xia Chen,Ming Wang 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.10

With the intensity of resource scarcities and environmental problems, the disposal and recovery of spent lithium-ion batteries, especially recovery of valuable metals, becomes vital. In this work, a method of co-extracting nickel, cobalt, manganese and being separated from lithium by single-stage solvent extraction is proposed. The extraction and separation process of D2EHPA was studied. The effects of extractant concentration, saponification percentage, extraction time and O:A on the extraction efficiency of D2EHPA were systematically studied. Nearly 100% manganese, 94% cobalt and about 90% nickel were co-extracted and separated from lithium using D2EHPA in kerosene by single-stage extraction. The maximum value of separation factors (Ni/Li, Co/Li and Mn/Li) was 13.03, 23.42 and 1904.24. The mathematical model of extraction of four ions was developed by combination of Levenberg-Marquardt method and Universal Global Optimization method. The proposed extraction model accurately fits the experimental data and helps to predict the extraction efficiency of each metal under the corresponding conditions.

Upregulation of miR-223 in the rat liver inhibits proliferation of hepatocytes under simulated microgravity

Yongjie Chen,Ji Xu,Chao Yang,Hongyu Zhang,Feng Wu,Jian Chen,Kai Li,Hailong Wang,Yu Li,Yinghui Li,Zhongquan Dai 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Long-term spaceflight affects numerous organ systems in the body, including metabolic dysfunction. Recently, ample evidence has demonstrated that the liver is a vulnerable organ during spaceflight. However, the changes in hepatocyte proliferation and cell cycle control under microgravity remain largely unexplored. In the present study, we first confirmed that the serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase, biochemical markers of liver function, were altered in rats under tail suspension (TS) conditions to simulate microgravity, as shown in previous reports. Next, we demonstrated that the cell proliferation activity, determined by Ki67, PCNA and PH3, was significantly decreased at the different TS time points (TS for 14, 28 and 42 days) compared with that in the control group. Consistently, the positive cell cycle regulators Ccna2, Ccnd1, Cdk1, Cdk2 and cyclin D3 were also significantly decreased in the TS groups as shown by quantitative real-time PCR and western blotting analysis. Subsequent analysis revealed that the aberrant hepatocyte proliferation inhibition under simulated microgravity was associated with the upregulation of miR-223 in the liver. We further found that miR-223 inhibited the proliferation of Hepa1-6 cells and identified CDK2 and CUL1 as its direct targets. In addition, the decreased expression of CDK2 and CUL1 was negatively correlated with the level of p27 in vitro and in vivo, which may have been responsible for retarding hepatocyte proliferation. Collectively, these data indicate that upregulation of miR-223 was associated with the inhibition of liver cell growth and reveal the role of miR-223 in rat hepatocyte proliferation disorders and the pathophysiological process under simulated microgravity.

Expression and Localization of Osteopontin in the Reproductive Tract of Male Hu Sheep

Shan Lan,Yongjie Wan,Feng Wang 한국동물생명공학회(구 한국동물번식학회) 2014 한국동물번식학회 한중일 심포지엄 Vol.2014 No.1

Expression of Mitochondria-Associated Genes (PGC-1α, NRF-1, BCL2 and BAX) in Follicular Development and Atresia of Goat Ovaries

Guoming Zhang,Yongjie Wan,Feng Wang 한국동물생명공학회(구 한국동물번식학회) 2014 한국동물번식학회 한중일 심포지엄 Vol.2014 No.1

Anterior Gradient 3 Promotes Breast Cancer Development and Chemotherapy Response

Qiao Xu,Ying Shao,Jinman Zhang,Huikun Zhang,Yawen Zhao,Xiaoli Liu,Zhifang Guo,Wei Chong,Feng Gu,Yongjie Ma 대한암학회 2020 Cancer Research and Treatment Vol.52 No.1

Purpose Anterior gradient 3 (AGR3) belongs to human anterior gradient (AGR) family. The function of AGR3 on cancer remains unknown. This research aimed to investigate if AGR3 had prognostic values in invasive ductal carcinoma (IDC) of breast cancer and could promote tumor progression. Materials and Methods AGR3 expression was detected in breast benign lesions, ductal carcinoma in situ and IDC by immunohistochemistry analysis. AGR3’s correlations with clinicopathological features and prognosis of IDC patients were analyzed. By cell function experiments, collagen gel droplet-embedded culture drug sensitivity test and cytotoxic analysis, AGR3’s impacts on proliferation, invasion ability, and chemotherapeutic drug sensitivity of breast cancer cells were also detected. Results AGR3 was up-regulated in luminal subtype of histological grade I-II of IDC patients and positively correlated with high risks of recurrence and distant metastasis. AGR3 high expression could lead to bone or liver metastasis and predict poor prognosis of luminal B. In cell lines, AGR3 could promote proliferation and invasion ability of breast cancer cells which were consistent with clinical analysis. Besides, AGR3 could indicate poor prognosis of breast cancer patients treated with taxane but a favorable prognosis with 5-fluoropyrimidines. And breast cancer cells with AGR3 high expression were resistant to taxane but sensitive to 5-fluoropyrimidines. Conclusion AGR3 might be a potential prognostic indicator in luminal B subtype of IDC patients of histological grade I-II. And patients with AGR3 high expression should be treated with chemotherapy regimens consisting of 5-fluoropyrimidines but no taxane.