Differential microRNA Expression by Solexa Sequencing in the Sera of Ovarian Cancer Patients

Ji, Ting,Zheng, Zhi-Guo,Wang, Feng-Mei,Xu, Li-Jian,Li, Lu-Feng,Cheng, Qi-Hui,Guo, Jiang-Feng,Ding, Xian-Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

MicroRNAs are a class of small noncoding RNA which play important regulatory roles in a variety of cancers. MiRNA-specific expression profiles have been reported for several pathological conditions. In this study, we combined large scale parallel Solexa sequencing to identify 11 up-regulated miRNAs and 19 down-regulated miRNAs with computational techniques in the sera of ovarian cancer patients while using healthy serum as the control. Among the above, four miRNAs (miR-22, miR-93, miR-106b, miR-451) were validated by quantitative RT-PCR and found to be significantly aberrantly expressed in the serum of ovarian cancer patients (P<0.05). There were no significant differences between samples from cancer stage I/II and III/IV. However, the levels of miR-106b (p=0.003) and miR-451 (p=0.007) were significantly different in those patients under and over 51 yearsof age. MiR-451 and miR-93 were also specific when analyzed with reference to different levels of CA125. This study shows that Solexa sequencing provides a promising method for cancer-related miRNA profiling, and selectively expressed miRNAs could be used as potential serum-based biomarkers for ovarian cancer diagnosis.

Varistor and dielectric properties of Cr2O3 doped SnO2eZn2SnO4 composite ceramics

Guo-Zhong Zang,Feng-Zi Zhou,Jing-Xiao Cao,Xiao-Fei Wang,Zhao-Wu Wang,Li-Ben Li,Guo-Rong Li 한국물리학회 2014 Current Applied Physics Vol.14 No.12

Cr2O3 doped SnO2eZn2SnO4 composite ceramics were prepared by traditional ceramic processing and the varistor, dielectric properties were investigated. With increasing Cr2O3 content, the breakdown electrical field EB increases from 11 to 92 V/mm and the relative dielectric constant εr measured at 1 kHz, 50 C decreases from 11,028 to 3412, respectively. The barrier height fB about 0.8e0.84 eV and the decreasing of SnO2 grain size suggest that the varistor behavior with high εr is originated from SnO2 eSnO2 or SnO2eZn2SnO4 grain boundary. In the dielectric spectra lower than 1 kHz, a dielectric peak is presented and depressed with increasing bias voltage. Similarly, at high temperature, the dielectric constant also presents a dielectric peak in the temperature spectra and the peak becomes faint with increasing frequency. The exhibition of the dielectric peak is thought to be attributed to the conduction of grain boundary since it is accompanied by the sharp increase of dielectric loss. In addition, a dielectric relaxation with the activation energy about 0.4e0.5 eV was observed in the temperature range of 20 e100 C. Based on the results, the formation mechanism of Schottky barriers at grain boundaries and the varistor behavior with high dielectric constant are well understood.

Research Articles : The Influence of Challenge on Cathepsin B and D Expression Patterns in the Silkworm Bombyx mori L

( Feng Yao Wu ),( Feng Ming Zou ),( Jun Qiang Jia ),( Sheng Peng Wang ),( Guo Zheng Zhang ),( Xi Jie Guo ),( Zhong Zheng Gui ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.23 No.1

Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that Bombyx mori cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RTPCR was performed to detect cathepsin gene expression at the transcription level when challenged by B. mori nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the 5th instar, and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the 5th instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.

A novel method for identifying rotor-stator rubbing positions using the cepstrum analysis technique

Guo Chen,Yong-Quan Liu,Guang-Yi Jiang,Cheng-Gang Li,Guo-Quan Feng,De-You Wang 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.9

A novel method of aero-engine rubbing positions identification based on cepstrum analysis is proposed, and the transfer path characteristicswhich reflect the transfer characteristics information from rubbing points to casing measuring points are separated from the vibrationacceleration signals of casing by means of cepstrum analysis. Therefore, there is different transfer characteristics information atdifferent rubbing positions, and in view of this, twenty rubbing positions identification features from the cepstrum are extracted. A largenumber of rubbing experiments of different positions are simulated with the rotor experiment rig of aero-engine, and the characteristicanalysis of experimental samples at different rubbing positions is carried out, and the results indicate the consistency of features to thesame rubbing position and the difference of the features to the different rubbing positions. Finally, the aero-engine rubbing positionsidentification is carried out using the nearest neighbor classification method, the recognition rate reaches 100%, and the effectiveness ofthe method is full verified.

Self-microemulsifying Drug Delivery System for Improved Oral Bioavailability of Dipyridamole: Preparation and Evaluation

Feng Guo,Haijun Zhong,Jing He, Baogang Xie,Fen Liu, Helin Xu,Minmin Liu,Chunlian Xu 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

Dipyridamole shows poor and variable bioavailability after oral administration due to pHdependent solubility, low biomembrane permeability as well as being a substrate of P-glycoprotein. In order to improve the oral absorption of dipyridamole, a self-microemulsifying drug delivery system (SMEDDS) for dipyridamole was prepared and evaluated in vitro and in vivo. The optimum formulation was 18% oleic acid, 12% Labrafac lipophile WL 1349, 42% Solutol HS 15 and 28% isopropyl alcohol. It was found that the performance of self-microemulsification with the combination of oleic acid and Labrafac lipophile WL 1349 increased compared with just one oil. The results obtained from an in vitro dissolution assay indicated that dipyridamole in SMEDDS dissolved rapidly and completely in pH 6.8 aqueous media, while the commercial drug tablet was less soluble. An oral bioavailability study in rats showed that dipyridamole in the SMEDDS formulation had a 2.06-fold increased absorption compared with the simple drug suspension. It was evident that SMEDDS may be an effective approach to improve the oral absorption for drugs having pH-dependent solubility.

Generic Constructions for Strong Designated Verifier Signature

Feng, Deng-Guo,Xu, Jing,Chen, Wei-Dong Korea Information Processing Society 2011 Journal of information processing systems Vol.7 No.1

A designated verifier signature is a special type of digital signature, which convinces a designated verifier that she has signed a message in such a way that the designated verifier cannot transfer the signature to a third party. A strong designated verifier signature scheme enhances the privacy of the signer such that no one but the designated verifier can verify the signer's signatures. In this paper we present two generic frame works for constructing strong designated verifier signature schemes from any secure ring signature scheme and any deniable one-pass authenticated key exchange protocol, respectively. Compared with similar protocols, the instantiations of our construction achieve improved efficiency.

Synthesis of Ordered Mesoporous Manganese Oxides by Double Replication for Use as an Electrode Material

Guo, Xiao-Feng,Kim, Geon-Joong Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.1

Periodically ordered mesoporous manganese oxides were synthesized in a single and double replication procedure. Mesoporous SBA-15 and -16 silica and their reverse replica carbons were successively used as hard templates. The silica and carbon pore systems were infiltrated with $Mn(NO_3)_2{\cdot}xH_2O$ or $Mn(AcAc)_2$, which was then converted to $Mn_2O_3$ at 873 K; the silica and carbon matrix were finally removed by NaOH solution or calcinations in air. The structure of the mesoporous $Mn_2O_3$, using a carbon template, corresponds to that of the original SBA-15 and SBA-16 silica. The products consist of hexagonally arranged cylindrical mesopores with crystalline pore walls or cubic mesoporous pores. The structure of replica has been confirmed by XRD, TEM analysis, and its electrochemical properties were tested with cyclic voltammetry. Formation of $Mn_2O_3$ inside the mesoporous carbon pore system showed much improved electrical properties.

An experimental research for the dynamic characteristic of air spring under the design load

Guo Feng,Jongwon Seok 대한기계학회 2018 대한기계학회 춘추학술대회 Vol.2018 No.5

Phenomenon of Entropic Stochastic Resonance with Asymmetric Dichotomous Noise and White Noise

Feng Guo,Shao-Fu Li,Xiao-Feng Cheng 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.60 No.3

The entropic stochastic resonance (ESR) in a confined system subject to asymmetric dichotomous noise, white noise, and a periodic square-wave signal is investigated. Under the adiabatic approximation condition, by use of the properties of the dichotomous noise, we obtain the expression of the output signal-to-noise ratio (SNR) based on two-state theory. The SNR is shown to be a nonmonotonic function of the strength and asymmetry of the dichotomous noise, the intensity of the white noise, and the amplitude of the square-wave signal. The SNR varies non-monotonically with increases in the parameters of the confined structure. The influence of the correlation rate of the dichotomous noise and the frequency of the external constant force on the SNR is also discussed.

Study on Global Optimization and Control Strategy Development for a PHEV Charging Facility

Feng Guo,Inoa, E.,Woongchul Choi,Jin Wang IEEE 2012 IEEE Transactions on Vehicular Technology VT Vol.61 No.6

<P>This paper provides a full study of a photovoltaic (PV)-aided plug-in hybrid electric vehicle (PHEV) charging facility by investigating the two most challenging technical issues: 1) sizing of the local energy storage (LES) unit and 2) control strategies of the facility. First, the paper proposes a method for determining the optimal size of LES for a charging facility. Second, the paper develops a control strategy for the integration of the PHEV charging stations with the proposed LES and PVs. The proposed LES-sizing method, which is based on optimal control theory, minimizes a cost function based on the average value of kilowatt-hour price, irradiance, and PHEVs' usage patterns. A power-loss/temperature-based battery model and a temperature-based charging strategy previously developed by the authors are utilized to determine the optimal LES size. Afterward, with the optimized facility parameters, a detailed circuit model of the charging facility, including PVs, PHEVs, and LES, is constructed with a real-time simulation system. While an experimental setup for this kind of complex and high-cost system was not readily feasible, real-time simulation was carried out to prove the effectiveness of the proposed control strategy. To validate the effectiveness and accuracy of the real-time simulation, control hardware-in-the-loop (HIL) and power-inverter-based experiments have been carried out at the subsystem level.</P>