Effects of Coated Compound Proteases on Apparent Total Tract Digestibility of Nutrients and Apparent Ileal Digestibility of Amino Acids for Pigs

Pan, L.,Zhao, P.F.,Yang, Z.Y.,Long, S.F.,Wang, H.L.,Tian, Q.Y.,Xu, Y.T.,Xu, X.,Zhang, Z.H.,Piao, X.S. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.12

Two experiments were conducted to evaluate effects of coated compound proteases (CC protease) on apparent total tract digestibility (ATTD) of nitrogen (N) and energy, and apparent ileal digestibility (AID) of amino acids (AA) and nutrients in diets for pigs. In Exp. 1, 12 crossbred barrows (initial body weight: $20.79{\pm}1.94kg$) were housed in individual metabolism crates and allotted into 2 treatments with 6 piglets per treatment according to weight in a randomized complete block design. The 2 diets were corn-soybean meal basal diets with (0.2 g/kg) or without CC protease supplementation. The CC protease supplementation increased (p<0.05) the digestible and metabolizable N and energy values and the digestibility and retention rate of N in the diet. The ATTD of energy and nutrients had been improved (p<0.05) in the diet supplemented with CC protease. In Exp. 2, 12 crossbred barrows (initial body weight: $20.79{\pm}1.94kg$), fitted with T-cannulas at the distal ileum, were blocked by body weight into 2 groups with 6 pigs each. The diets were the same as those in Exp. 1. The CC protease increased (p<0.05) the AID of crude protein and some essential AA including arginine, isoleucine and leucine. The AID and ATTD of energy and nutrients had been improved (p<0.05) by supplemental CC protease, but the hindgut digestibility of nutrients was unaffected. Overall, the CC protease improved the ATTD of N and energy and AID of some indispensible AA and nutrients in the corn-soybean meal diet for pigs. Therefore, the CC protease supplement could improve the utilization of protein in the corn-soybean meal diet and thus contribute to lower N excretion to the environment.

Partial cooperative spectrum sensing schedule in cognitive network

Xu, F.,Zheng, X.,Li, X.,Zhou, Z. SPRINGER SCIENCE + BUSINESS MEDIA 2009 Science China Information Sciences Vol.52 No.12

Effect of Dietary Cadmium Levels on Nutrient Digestibility and Retention of Iron, Copper and Zinc in Tissues of Growing Pigs

Han, X.Y.,Xu, Z.R.,Wang, Y.Z.,Tao , X.,Li, W.F. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.7

This experiment was conducted to investigate the effect of cadmium levels on weight gain, nutrient digestibility and the retention of iron, copper and zinc in tissues of growing pigs. A total of one hundred and ninety-two crossbred pigs (barrows, Duroc$\times$Landrace$\times$Yorkshine, 27.67$\pm$1.33 kg of average initial body weight) were randomly allotted to four treatments. Each treatment had three replicates with 16 pigs per pen. The corn-soybean basal diets were supplemented with 0, 0.5, 5.0, 10.0 mg/kg cadmium respectively, and the feeding experiment lasted for eight-three days. Cadmium chloride was used as cadmium source. The results showed that pigs fed the diet containing 10.0 mg/kg cadmium had lower ADG and FCR than any other treatments (p<0.05). Apparent digestibility of protein in 10.0 mg/kg cadmium-treated group was lower than that of other groups (p<0.05). There was lower iron retention in some tissues of 5.0 mg/kg and 10.0 mg/kg cadmium treatments (p<0.05). However, pigs fed the diet 10.0 mg/kg cadmium had higher copper content in most tissues than that of any other groups (p<0.05). There was a significantly increase of zinc retention in kidney of 10.0 mg/kg cadmium additional group (p<0.05) and zinc concentrations in lymphaden, pancreas and heart of 10.0 mg/kg cadmium treatment were lower than those of the control (p<0.05). This study indicated that relatively high cadmium level (10.0 mg/kg) could decrease pig growth performance and change the retention of iron, copper and zinc in most tissues during extended cadmium exposure period.

Effects of Arsenic (As^III) on Lipid Peroxidation, Glutathione Content and Antioxidant Enzymes in Growing Pigs

Wang, L.,Xu, Z.R.,Jia, X.Y.,Jiang, J.F.,Han, X.Y. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.5

This experiment was conducted to investigate the effect of arsenic ($As^{III}$) on lipid peroxidation, glutathione content and antioxidant enzymes in growing pigs. Ninety-six Duroc-Landrace-Yorkshire crossbred growing pigs (48 barrows and 48 gilts, respectively) were randomly assigned to four groups and each group was randomly assigned to three pens (four barrows and four gilts). The four groups received the same corn-soybean basal diet which was supplemented with 0, 10, 20, 30 mg/kg As respectively. Arsenic was added to the diet in the form of $As_2O_3$. The experiment lasted for seventy-eight days after a seven-day adaptation period. Malondialdehyde (MDA) levels, glutathione (GSH) contents and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) activities were analyzed in serum, livers and kidneys of pigs. The results showed that pigs treated with 30 mg As/kg diet had a decreased average daily gain (ADG) (p<0.05) and an increased feed/gain ratio (F/G) (p<0.05) compared to the controls. The levels of MDA significantly increased (p<0.05), and the contents of GSH and the activities of SOD, CAT, GPx, GR and GST significantly decreased (p<0.05) in the pigs fed 30 mg As/kg diet. The results indicated that the mechanism of arsenic-induced oxidative stress in growing pigs involved lipid peroxidation, depletion of glutathione and decreased activities of some enzymes, such as SOD, CAT, GPx, GR and GST, which are associated with free radical metabolism.

Association Analyses with Carcass Traits in the Porcine KIAA1717 and HUMMLC2B Genes

Xu, D.Q.,Xiong, Y.Z.,Liu, M.,Lan, J.,Ling, X.F.,Deng, C.Y.,Jiang, S.W. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.11

By screening a subtracted cDNA library constructed with mRNA obtained from the longissimus dorsi muscles of F1 hybrids Landrace${\times}$Yorkshire and their Yorkshire female parents, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. In the present work we investigated two SNPs, one (C1354T) at the 3' untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, in a resource population derived from crossing Chinese Meishan and Large White pig. The selected pigs were genotyped by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with thorax-waist backfat thickness (p<0.05), buttock backfat thickness (p<0.05), average backfat thickness (p<0.05), loin eye height (p<0.05), loin eye area (p<0.05), carcass length to 1$^{st}$ spondyle (p<0.01) and carcass length to 1st rib (p<0.01). HUMMLC2B A345G were significantly associated with loin eye width (p<0.05), loin eye area (p<0.05). Further studies are needed to confirm these preliminary results.

Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Oviduct-specific Glycoprotein 1 Locus is Associated with Litter Size and Weight of Ovaries in Pigs

Niu, B.Y.,Xiong, Y.Z.,Li, F.E.,Jiang, S.W.,Deng, C.Y.,Ding, S.H.,Guo, W.H.,Lei, M.G.,Zheng, R.,Zuo, B.,Xu, D.Q.,Li, J.L. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.5

Oviduct-specific glycoprotein 1 (OVGP1) is implicated in playing a role in fertilization and early embryo development. In this study, we have obtained the sequence of intron 9 of OVGP1 gene in swine. Comparative sequencing of Meishan (a native Chinese breed) and Large White pig breeds revealed an A/T substitution at position 943. A PCR-EcoRI-RFLP assay was developed to detect this mutation. Polymorphism analysis in Qingping animals showed that pigs with BB genotype had lower number of piglets born alive (NBA) in multiple parities than pigs with AA (p<0.05) and AB genotype (p<0.01). In Large $White{\times}Meishan$ ($LW{\times}M$) $F_2$ offspring, the weight of both ovaries (OW) of the BB genotype was significantly lighter than that of AB (p = 0.05) and AA (p<0.01) genotypes. Analysis of the data also revealed that the mutation locus affected these two traits mostly by additive effects. These studies indicated that the polymorphism was associated with NBA and OW in two distinct populations and further investigations in more purebreds or crossbreds are needed to confirm these results.

The continuous casting of a semisolid aluminum alloy billet with a multiple magnetic field imposed

Z.F. Zhang,Z.F. Tian,L.K. Shi,J. Xu 한양대학교 세라믹연구소 2006 Journal of Ceramic Processing Research Vol.7 No.2

In order to obtain semi-solid aluminum alloy billets of high quality, an investigation was carried out of imposing multiple magnetic fields from the outside of a cold-crucible copper mold in a continuous casting process. AlSi6Mg2 (wt.%) alloy designed for a semi-solid metal (SSM) process was continuously cast through a submerged entry nozzle under various conditions. By means of optimizing the distribution of multiple magnetic fields in the mold, the effect of multiple magnetic fields on both the meniscus motion and billet quality was examined. The experimental results showed that meniscus disturbance could be controlled efficiently and the surface quality of a semi-solid aluminum alloy billet was improved greatly by imposing multiple magnetic fields, and also a uniformly fine, globular microstructure across the transverse section of the billet was achieved by optimizing the distribution of the multiple magnetic field.

First observation ofγrays emitted from excited states south-east ofSn132: Theπg9/2−1⊗ν_f7/2multiplet ofIn83132

Jungclaus, A.,Gargano, A.,Grawe, H.,Taprogge, J.,Nishimura, S.,Doornenbal, P.,Lorusso, G.,Shimizu, Y.,Simpson, G. S.,Sö,derströ,m, P.-A.,Sumikama, T.,Xu, Z. Y.,Baba, H.,Browne, F.,Fukuda, N.,G American Physical Society 2016 Physical Review C Vol.93 No.4

<P>For the first time, the gamma decay of excited states has been observed in a nucleus situated in the quadrant south-east of doubly magic Sn-132, a region in which experimental information so far is limited to ground-state properties. Six gamma rays with energies of 50, 86, 103, 227, 357, and 602 keV were observed following the beta-delayed neutron emission from Cd-133(85), populated in the projectile fission of a U-238 beam at the Radioactive Isotope Beam Factory at RIKEN within the EURICA project. The new experimental information is compared to the results of a modern realistic shell-model calculation, the first one in this region very far from stability, focusing in particular on the pi 0g(9/2)(-1) circle times nu 1f(7/2) particle-hole multiplet in In-132(83). In addition, theoretical estimates based on a scaling of the two-body matrix elements for the pi h(11/2)(-1) circle times nu g(9/2) analog multiplet in Tl-208(127), one major proton and one major neutron shell above, are presented.</P>

Effects of Replacing Soybean Meal with Fermented Rapeseed Meal on Performance, Serum Biochemical Variables and Intestinal Morphology of Broilers

Xu, F.Z.,Zeng, X.G.,Ding, X.L. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.12

This trial was performed to study the effects of replacing soybean meal (SBM) with fermented rapeseed meal (RSM) on growth performance, serum biochemistry variable and intestinal morphology of broilers. A total of 640 d-old Arbor Acres broiler chicks were randomly allocated to 4 dietary treatments, 4 pens per treatment and 40 birds per pen for a 6-wk feeding trial. In the four treatment groups, fermented RSM replaced soybean meal at 0, 5, 10, and 15%, respectively. On 21 d and 42 d, two birds from each pen were randomly selected and slaughtered. Blood samples and sections of duodenum, jejunum, and ileum were collected for measurement of serum biochemical variables and intestinal morphology, respectively. Results showed that body weight gain (BWG) and feed conversion (FC) were significantly (p<0.01) poorer for birds fed the 15% fermented RSM diet than those fed with 0, 5 and 10% fermented RSM diets during all periods. Compared with 0 and 5% fermented RSM groups, IgG content in the serum of birds in 10 and 15% fermented RSM groups was improved (p<0.01) urea nitrogen content of serum was reduced (p<0.01) during both growing and finishing periods. However, IgM, phosphorus and calcium levels increased (p<0.05) only during the growing period. Increased (p<0.05) villus height was observed in the duodenum and jejunum of broilers fed the diet with 10% fermented RSM. In addition, villus height to crypt depth ratio in the jejunum was significantly higher (p<0.01) for birds fed the diet with 10% fermented RSM than for those fed diets with 0, 5 and 15% fermented RSM. The present results suggest that RSM fermented with Lactobacillus fermentum and Bacillus subtilis is a promising alternative protein source and that it could be safely used replace up to 10% SBM in broiler diets.