Effect of Thiol-reducing Agents and Antioxidants on Sulfasalazine-induced Hepatic Injury in Normotermic Recirculating Isolated Perfused Rat Liver

Heidari, Reza,Esmailie, Neda,Azarpira, Negar,Najibi, Asma,Niknahad, Hossein Korean Society of ToxicologyKorea Environmental Mu 2016 Toxicological Research Vol.32 No.2

Sulfasalzine is a widely administered drug against inflammatory-based disorders in human. However several cases of liver injury are associated with its administration. There is no stabilized safe protective agent against sulfasalazine-induced liver injury. Current investigation was designed to evaluate if N-acetylcysteine (NAC) and dithioteritol (DTT) as thiol reducing agents and/or vitamins C and E as antioxidants have any protective effects against sulfasalazine-induced hepatic injury in an ex vivo model of isolated rat liver. Rat liver was canulated and perfused via portal vein in a closed recirculating system. Different concentrations of sulfasalazine and/or thiol reductants and antioxidants were administered and markers of organ injury were monitored at different time intervals. It was found that 5 mM of sulfasalazine caused marked liver injury as judged by rise in liver perfusate level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) (p < 0.05). A significant amount of lipid peroxidation and hepatic glutathione depletion were detected in drug-treated livers, accompanied with significant histopathological changes of the organ. Administration of NAC ($500{\mu}M$), DTT (${400\mu}M$), Vitamin C ($200{\mu}M$), or vitamin E ($200{\mu}M$) significantly alleviated sulfasalazine-induced hepatic injury in isolated perfused rat liver. The data obtained from current investigation indicate potential therapeutic properties of thiol reductants and antioxidants against sulfasalazine-induced liver injury.

Effect of Thiol-reducing Agents and Antioxidants on Sulfasalazine-induced Hepatic Injury in Normotermic Recirculating Isolated Perfused Rat Liver

Reza Heidari,Neda Esmailie,Negar Azarpira,Asma Najibi,Hossein Niknahad 한국독성학회 2016 Toxicological Research Vol.32 No.2

Sulfasalzine is a widely administered drug against inflammatory-based disorders in human. However several cases of liver injury are associated with its administration. There is no stabilized safe protective agent against sulfasalazine-induced liver injury. Current investigation was designed to evaluate if N-acetylcysteine (NAC) and dithioteritol (DTT) as thiol reducing agents and/or vitamins C and E as antioxidants have any protective effects against sulfasalazine-induced hepatic injury in an ex vivo model of isolated rat liver. Rat liver was canulated and perfused via portal vein in a closed recirculating system. Different concentrations of sulfasalazine and/or thiol reductants and antioxidants were administered and markers of organ injury were monitored at different time intervals. It was found that 5 mM of sulfasalazine caused marked liver injury as judged by rise in liver perfusate level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) (p < 0.05). A significant amount of lipid peroxidation and hepatic glutathione depletion were detected in drug-treated livers, accompanied with significant histopathological changes of the organ. Administration of NAC (500 μM), DTT (400 μM), Vitamin C (200 μM), or vitamin E (200 μM) significantly alleviated sulfasalazine-induced hepatic injury in isolated perfused rat liver. The data obtained from current investigation indicate potential therapeutic properties of thiol reductants and antioxidants against sulfasalazine-induced liver injury.

Evaluation of the Pathogenesis of Tumor Development from Endometriosis by Estrogen Receptor, P53 and Bcl-2 Immunohistochemical Staining

Esmaili, Haidarali,Vahedi, Amir,Mohajeri, Shiva,Mostafidi, Elmira,Azimpouran, Mahzad,Behzad, Mohammad Naghavi Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.12

Objective: Endometriosis, one of the most common estrogen dependent gynecological disorders, can present as both benign and malignant disease. The prevalence of tumoral transformation is 0.7-1.6% and the most common tumors are clear cell and endometrioid carcinomas. Unfortunately, the pathogenesis of transformation is unknown. For this purpose, we examined molecular alterations in ovarian endometriosis and endometriosis-associated tumors. Methods: Using the data bank of Alzahra hospital pathology department and paraffin blocks from appropriate cases were identified. Sections were cut and stained for 3 markers: estrogen receptor, P53 and bcl2. Correlations between findings were investigated. Results: Nineteen cases of endometriosis-associated tumor and 19 cases of endometriosis were identified. Staining for bcl2 was documented in 14 of 19 (73.7%) of endometriosis-associated tumor cases and also 7 of 19 (36.8%) endometriosis cases (P=0.02). Only 3 of the 19 (15.8%) endometriosis-associated tumors exhibited positive staining for estrogen receptors, compared with 14 of 19 (73.7%) endometriosis cases (P<0.001). Positive staining for P53 was noted in 5 of 19 (31.6%) endometriosis-associated ovarian tumor samples but was absent in endometriosis samples (0%), (P =0.008). Conclusions: Endometriosis-associated tumors appear to be associated with overexpression of bcl2 and P53 and reduced expression of Estrogen receptor. These finding may help to diagnose tumoral transformation with a background of endometriosis.

Improved Region-Based TCTL Model Checking of Time Petri Nets

Esmaili, Mohammad Esmail,Entezari-Maleki, Reza,Movaghar, Ali Korean Institute of Information Scientists and Eng 2015 Journal of Computing Science and Engineering Vol.9 No.1

The most important challenge in the region-based abstraction method as an approach to compute the state space of time Petri Nets (TPNs) for model checking is that the method results in a huge number of regions, causing a state explosion problem. Thus, region-based abstraction methods are not appropriate for use in developing practical tools. To address this limitation, this paper applies a modification to the basic region abstraction method to be used specially for computing the state space of TPN models, so that the number of regions becomes smaller than that of the situations in which the current methods are applied. The proposed approach is based on the special features of TPN that helps us to construct suitable and small region graphs that preserve the time properties of TPN. To achieve this, we use TPN-TCTL as a timed extension of CTL for specifying a subset of properties in TPN models. Then, for model checking TPN-TCTL properties on TPN models, CTL model checking is used on TPN models by translating TPN-TCTL to the equivalent CTL. Finally, we compare our proposed method with the current region-based abstraction methods proposed for TPN models in terms of the size of the resulting region graph.

Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes

Hamed Esmaili,Mohsen Eslami,Hamed Khalilvandi-Behrozyar,Farhad Farrokhi-Ardabili 한국축산학회 2023 한국축산학회지 Vol.65 No.5

The current study was designed to evaluate the effect of sequential low and high dietary linseed oil (LO; as omega-3 enriched fatty acid; FA) before and post insemination, respectively, on different plasma variables of ewes. Fat-tailed Qezel ewes were assigned randomly to be fed a diet enriched with 3% LO (n = 30) or the saturated FA (SFA; n = 30) three weeks before insemination (Day 0). The lipogenic diet supplemented with 6% LO or SFA was fed after insemination until Day +21. The control ewes were fed an isocaloric and isonitrogenous diet with no additional FA during the study. Estrus was synchronized by inserting a vaginal sponge (Spongavet®) for 12 days + 500 IU equine chorionic gonadotropin (eCG; Gonaser®), and ewes were inseminated via laparoscopic approach 56–59 h after eCG injection. The size of ovarian structures was assessed by transvaginal ultrasonography at −21, −14, −2, 0, and +10 days. Blood samples were collected weekly to measure the plasma’s different biochemical variables and FA profile. Treatment did not affect the amounts of glucose, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, interleukin-10, interleukin-2, and non-esterified FA (p > 0.05). Conversely, concentrations of triglyceride, cholesterol, tumor necrosis factor-alpha, and insulin-like growth factor-1 were higher in SFA-fed ewes relative to control animals (p < 0.05). LO feeding resulted in greater amounts of n-3 FA isomers in plasma, while higher amounts of stearic acid were detected in SFA fed group 0 and +21 (p < 0.05). The number of ovarian follicles and corpora lutea also were not affected by treatment. Other reproductive variables were not affected by treatment except for the reproductive rate. It seems that LO or SFA feeding of fat-tailed ewes peri-insemination period was not superior to the isocaloric non-additional fat diet provided for the control group during the non-breeding season.

Improved Region-Based TCTL Model Checking of Time Petri Nets

Mohammad Esmail Esmaili,Reza Entezari-Maleki,Ali Movaghar 한국정보과학회 2015 Journal of Computing Science and Engineering Vol.9 No.1

The most important challenge in the region-based abstraction method as an approach to compute the state space of time Petri Nets (TPNs) for model checking is that the method results in a huge number of regions, causing a state explosion problem. Thus, region-based abstraction methods are not appropriate for use in developing practical tools. To address this limitation, this paper applies a modification to the basic region abstraction method to be used specially for computing the state space of TPN models, so that the number of regions becomes smaller than that of the situations in which the current methods are applied. The proposed approach is based on the special features of TPN that helps us to construct suitable and small region graphs that preserve the time properties of TPN. To achieve this, we use TPN-TCTL as a timed extension of CTL for specifying a subset of properties in TPN models. Then, for model checking TPN-TCTL properties on TPN models, CTL model checking is used on TPN models by translating TPN-TCTL to the equivalent CTL. Finally, we compare our proposed method with the current region-based abstraction methods proposed for TPN models in terms of the size of the resulting region graph.

Adaptive group of ink drop spread: a computer code to unfold neutron noise sources in reactor cores

Seyed Abolfazl Hosseini,Iman Esmaili Paeen Afrakoti 한국원자력학회 2017 Nuclear Engineering and Technology Vol.49 No.7

The present paper reports the development of a computational code based on the Adaptive Group of InkDrop Spread (AGIDS) for reconstruction of the neutron noise sources in reactor cores. AGIDS algorithmwas developed as a fuzzy inference system based on the active learning method. The main idea of theactive learning method is to break a multiple inputesingle output system into a single inputesingleoutput system. This leads to the ability to simulate a large system with high accuracy. In the presentstudy, vibrating absorber-type neutron noise source in an International Atomic Energy Agency-twodimensional reactor core is considered in neutron noise calculation. The neutron noise distribution inthe detectors was calculated using the Galerkin finite element method. Linear approximation of theshape function in each triangle element was used in the Galerkin finite element method. Both the realand imaginary parts of the calculated neutron distribution of the detectors were considered input data inthe developed computational code based on AGIDS. The output of the computational code is thestrength, frequency, and position (X and Y coordinates) of the neutron noise sources. The calculatedfraction of variance unexplained error for output parameters including strength, frequency, and X and Ycoordinates of the considered neutron noise sources were 0.002682 #/cm3s, 0.002682 Hz, and0.004254 cm and 0.006140 cm, respectively.

A New Controller Design for Power System Stability Enhancement

A. Khodabakhshian,R. Hooshmand,M. Esmaili 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10

This paper uses the Quantitative Feedback Theory (QFT) to design a new robust Power System Stabilizer (PSS) able to provide acceptable damping over a wide range of operating points. In the design procedure the main purpose is to reject the load fluctuations and, therefore, a particular transfer function P(s)=(Δω/ΔTm) is used as the nominal plant. The parametric uncertainty in power systems has been easily handled by using QFT. Results clearly show the benefits of the proposed controller for stability enhancement of power systems.

Perceived Psychological Traumatic Childbirth in Iranian Mothers: Diagnostic Value of Coping Strategies

Sedigheh Abdollahpour,Ahmad Khosravi,Habibollah Esmaily,Seyed Abbas Mousavi 질병관리본부 2019 Osong Public Health and Research Persptectives Vol.10 No.2

Objectives: The aim of this study was to investigate the diagnostic value of a stress coping scale for predicting perceived psychological traumatic childbirth in mothers. Methods: This cross-sectional study was performed on 400 new mothers (within 48 hours of childbirth). Psychological traumatic childbirth was evaluated using the 4 diagnostic criteria of Diagnostic and Statistical Manual of Mental Disorders. Coping was measured using Moss and Billings’ Stress Coping Strategies Scale. Results: The overall mean score of stress coping was 29 ± 14.2. There were 193 (43.8%) mothers that had experienced a psychological traumatic childbirth. A stress coping score ≤ 30, with a sensitivity of 90.16 (95% CI = 85.1-94.0), and a specificity of 87.44 (95% CI = 82.1-91.6), was determined as a predictor of psychological traumatic childbirth. So that among mothers with stress coping scores ≤ 30, 87% had experienced a psychological traumatic childbirth. Conclusion: Investigating the degree of coping with stress can be used as an accurate diagnostic tool for psychological traumatic childbirth. It is recommended that during pregnancy, problem-solving and stress management training programs be used as psychological interventions for mothers with low levels of stress control. This will ensure that they can better cope with traumatic childbirth and posttraumatic stress in the postpartum stage.

Self-Disclosure of Breast Cancer Diagnosis by Iranian Women to Friends and Colleagues

Najmabadi, Khadijeh Mirzaii,Azarkish, Fatemeh,Latifnejadroudsari, Robab,Shandiz, Fatemeh Homaei,Aledavood, Seyed Amir,Kermani, Ali Taghizadeh,Esmaily, Habib Ollah Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Background: Breast cancer (BC) is the most common form of cancer in Iranian women, and it remains a major health problem. An increasing number of young women are being diagnosed with BC, and therefore, there is an increasing likelihood that more women will survive breast cancer for many years. Many opine that self-disclosure of BC diagnosis is important because talking about cancer helps people to make sense of their experiences; in fact, self-disclosure appears to play an important role in many health outcomes. However, this has not yet been studied in BC patients in Iran. Therefore, this study aimed to explore the status of self-disclosure of BC diagnosis by Iranian women to friends and colleagues. Materials and Methods: All BC records for 2001-2011 of employed women were studied at five hospitals in Mashhad. Data about the self-disclosure of BC diagnosis were gathered through telephone interviews, and the participants filled out a questionnaire about their status of self-disclosure of BC diagnosis to various groups of people. Results: The mean age of employed women at the time of diagnosis was $44.3{\pm}6.7$ years. Over 60% self-disclosed to work colleagues and over 90% to bosses/managers. Seventy per cent reported that they had support from their family and husband's family, while 95% reported that they had support from parents, siblings, children and friends. Conclusions: Most employed women self-disclosed freely to family, friends, colleagues and bosses/managers. Apparently, self-disclosure of breast cancer diagnosis may have negative effects at work. About half of patients reported that they had support from family, managers and colleagues; however, for nearly 28% of employed women, disclosure had less positive effects. In particular, it altered their perception of others, produced difficulties with work and family and diminished closeness with the people who were told. However, the stigma of BC is far less than it once was.