http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Corni fructus ameliorates menopause symptom in 4-vinylcyclohexene diepoxide
Shin, Dasom,Koh, Myeong Shin,Lee, Sangchul,Lee, Da Hye,Kang, Geun-Hyung,Kim, Sejun,Jang, Dae Sik,Hwang, Deok-Sang,Kim, Youn-Sub,Bae, Hyunsu THE KOREAN SOCIETY OF TOXICOGENOMICS AND TOXICOPRP 2018 MOLECULAR AND CELLULAR TOXICOLOGY Vol. No.
Menopause is considered to mean falling levels of ovarian estradiol and progesterone. Menopause syndrome can lead to hot flashes, headaches, and depression etc. Corni Fructus (CF) was used as a therapeutic herbal medicine for treating various diseases such as pain, diabetic nephropathy and tuberculosis. This study was conducted to evaluate the protective effects of CF in follicle depletion of a mice model using the industrial chemical 4-vinylcyclohexene diepoxide (VCD). VCD directly targets prenatal follicles. Mice were injected with VCD (160 mg/kg) daily for 15 days and administered CF 18 times after VCD injection. CF treatment reduced body weight, tail skin temperature, and uterus weight. In addition, CF treatment significantly decreased total cholesterol, low-density lipoprotein, low-density lipoprotein/high-density lipoprotein ratio, and osteocalcin levels as well as correcting skin temperature. These results support the therapeutic potential of CF for those who suffer from menopause symptoms.
Corni fructus ameliorates menopause symptom in 4-vinylcyclohexene diepoxide
Dasom Shin,Myeong Shin Koh,Sangchul Lee,Da Hye Lee,Geun-Hyung Kang,Sejun Kim,장대식,Deok-Sang Hwang,Youn-Sub Kim,배현수 대한독성 유전단백체 학회 2018 Molecular & cellular toxicology Vol.14 No.1
Menopause is considered to mean falling levels of ovarian estradiol and progesterone. Menopause syndrome can lead to hot flashes, headaches, and depression etc. Corni Fructus (CF) was used as a therapeutic herbal medicine for treating various diseases such as pain, diabetic nephropathy and tuberculosis. This study was conducted to evaluate the protective effects of CF in follicle depletion of a mice model using the industrial chemical 4-vinylcyclohexene diepoxide (VCD). VCD directly targets prenatal follicles. Mice were injected with VCD (160 mg/kg) daily for 15 days and administered CF 18 times after VCD injection. CF treatment reduced body weight, tail skin temperature, and uterus weight. In addition, CF treatment significantly decreased total cholesterol, low-density lipoprotein, low-density lipoprotein/high-density lipoprotein ratio, and osteocalcin levels as well as correcting skin temperature. These results support the therapeutic potential of CF for those who suffer from menopause symptoms.
Dasom Shin,Hui-Seung Kang,Hyung Soo Kim,문귀임 한국식품위생안전성학회 2020 한국식품위생안전성학회지 Vol.35 No.4
In this work, we developed an analytical method for determining 11 illicit compounds in dietary supplements using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry. Eleven target compounds, including those meant for weight loss (7-keto-dihydroepiandrosterone, buformin, metformin, phenformin, salbutamol, and tolbutamide), sexual enhancement (dihydroepiandrosterone), and relaxation (asarone, kavain, magnoflorine, and picamilon) were screened and confirmed in dietary supplements. Method validation was performed by evaluating the selectivity, linearity, limit of quantification (LOQ), accuracy, and precision according to the Association of Official Analytical Chemists guidelines. The linearity was > 0.993 for all analytes.The LOQs were ranged in 2.1-9.9 μg/mL (HPLC-DAD) and 0.002-0.008 μg/mL (LC-MS/MS). The accuracies (expressed as recovery) were 90.0-106% (HPLC-DAD) and 83.0-114% (LC-MS/MS). The precision (expressed as the relative standard deviation) was below 10% using HPLC and LC-MS/MS. The proposed method can be used for the surveillance of illicit compounds in dietary supplements.
Shin, Areum,Lee, Eunjung,Jeon, Dasom,Park, Young-Guen,Bang, Jeong Kyu,Park, Yong-Sun,Shin, Song Yub,Kim, Yangmee American Chemical Society 2015 Biochemistry Vol.54 No.25
<P>Antimicrobial peptides (AMPs) are important components of the host innate immune system. Papiliocin is a 37-residue AMP purified from larvae of the swallowtail butterfly <I>Papilio xuthus</I>. Magainin 2 is a 23-residue AMP purified from the skin of the African clawed frog <I>Xenopus laevis</I>. We designed an 18-residue hybrid peptide (PapMA) incorporating N-terminal residues 1–8 of papiliocin and N-terminal residues 4–12 of magainin 2, joined by a proline (Pro) hinge. PapMA showed high antimicrobial activity but was cytotoxic to mammalian cells. To decrease PapMA cytotoxicity, we designed a lysine (Lys) peptoid analogue, PapMA-k, which retained high antimicrobial activity but displayed cytotoxicity lower than that of PapMA. Fluorescent dye leakage experiments and confocal microscopy showed that PapMA targeted bacterial cell membranes whereas PapMA-k penetrated bacterial cell membranes. Nuclear magnetic resonance experiments revealed that PapMA contained an N-terminal α-helix from Lys<SUP>3</SUP> to Lys<SUP>7</SUP> and a C-terminal α-helix from Lys<SUP>10</SUP> to Lys<SUP>17</SUP>, with a Pro<SUP>9</SUP> hinge between them. PapMA-k also had two α-helical structures in the same region connected with a flexible hinge residue at Nlys<SUP>9</SUP>, which existed in a dynamic equilibrium of <I>cis</I> and <I>trans</I> conformers. Using lipopolysaccharide-stimulated RAW264.7 macrophages, the anti-inflammatory activity of PapMA and PapMA-k was confirmed by inhibition of nitric oxide and inflammatory cytokine production. In addition, treatment with PapMA and PapMA-k decreased the level of ultraviolet irradiation-induced expression of genes encoding matrix metalloproteinase-1 (MMP-1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in human keratinocyte HaCaT cells. Thus, PapMA and PapMA-k are potent peptide antibiotics with antimicrobial and anti-inflammatory activity, with PapMA-k displaying enhanced bacterial selectivity.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2015/bichaw.2015.54.issue-25/acs.biochem.5b00392/production/images/medium/bi-2015-00392x_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bi5b00392'>ACS Electronic Supporting Info</A></P>