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간암과 간세포이형성 부위의 철침착 저항성에 대한 조직학적 연구
조욱행,강대영 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1
Liver cell dysplasia(LCD) is commonly associated with some liver diseases such as liver cirrhosis or hepatocellular carcinoma and has been regarded as a premaligant condition. In order to clarify the question of whether or not LCD is a premalignant liver lesion, iron special staining, Prussian blue, was done. The specimens were collected at the department of pathology, Chungnam National University College of Medicine from January 1983 to September 1992. The results were as follows: 1. Of observed 48 cases, 34 were hepatocellular carcinoma(70.8%), 11 were metastatic carcinoma(22.9%), and 3 were hepatoblastoma(6.3%). 2. LCD was present in 26 of 34 cases(76.5%) of hepatocellular carcinoma, 5 of 11 cases(45.5%) of metastatic carcinoma, and 1 of 3 case(33.3%) of hepatoblastoma. 3. The carcinoma cells were free of iron deposition(iron excluding foci) in contrast to the neighboring dysplastic or nonneoplastic normal hepatocytes. 4. The presence of iron deposition in LCD cases was similar to or more severe than that of normal hepatocytes. In summary, the results obtained from the present study indicate that LCD reflects neither a regenerating condition nor a premalignant lesion but suggests a reactive change or accompaning lession.
免疫組織化學的 方法에 依한 마우스組織에서의 Toxoplasma 抗原 檢出에 關한 硏究
趙裕行,李英河,羅榮彦,辛大煥 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1
This study was performed to identify the Toxoplasma antigens from the mice tissue chronically infected with Toxoplasma gondii Beverley strain by peroxidase-antiperoxidase(PAP) method. The 21 mice divided into two groups, control group (3mice) and cortisone-treated group (18mice). In cortisone-treated group, the mice were injected with 0.5mg of cortisone acetate twice a week for 6 week subcutaneously and 3 mice were sacrified at each week. The brain, lung, heart, liver, spleen, kidney, small intestine and skeletal muscle of mice were removed immediately after death. The removed tissues were fixed with 10% buffered neutral formalin, and then PAP method and H-E staining were done. The results were obtained as follows: 1. The cysts were found in the brain entirely, in the heart at 4 weeks, and in the skeletal muscle at 5 weeks. The number and size of cysts in the brain were increased in proportion to total dose of cortisone acetate in mice. Tachyzoites of Toxoplasma gondii showed at 4, 5 and 6 week in brain. 2. The brain, speen and skeletal muscle showed highly positive immunoreactivity. The liver, heart and small intestine were moderately positive, but lung and kidney showed weekly positive immunoreactivity by PAP method. 3. There were a chronic inflammatory responses in the brain, liver and heart but the remaining organs were almost normal appearance. And tachyzoites were found in the brain, associated with severe inflammatory changes. The present study revealed that tachyzoites and immunoreactivity of Toxoplasma were easily detected by PAP immune complex method.
Dae Haeng Cho,신수정,상병인,Moon-Ho Eom,김용환 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.5
ABE (acetone-butanol-ethanol) was produced through alkaline pre-hydrolysis, enzymatic saccharification,and fermentation using yellow poplar as a raw material. In alkaline pre-hydrolysis, 51.1% of the biomass remained as a residue. In the main woody components, the degrees of lignin and xylan removal were 94.3 and 62.0%, respectively. A yield of 80.9% for cellulose-to-glucose and 81.2% for xylan-to-xylose were obtained by enzymatic hydrolysis. The sugar composition of enzymatic hydrolysate was 95.1 g/L of glucose and 21.4 g/L of xylose. The enzymatic hydrolysate also contained 0.5 g/L of acetic acid and 0.5 g/L of total phenolics. Furfural and 5-hydroxymethylfurfural (5-HMF) were not detected in this hydrolysate. The yellow poplar hydrolysate (YPH) from enzymatic saccharification was used for the production of ABE using Clostridium acetobutylicum and C. beijerinckii. In YPH fermentation,C. acetobutylicum produced 18.1 g/L total ABE (productivity 0.38 g/L h, and yield 0.42), and C. beijerinckii produced 12.1 g/L (productivity 0.25 g/L h, and yield 0.37). Although the ABE productivity by C. beijerinckii was slightly low,the general performance of ABE fermentation in YPH was similar to or higher than those reported previously. Therefore, alkaline pre-hydrolysis could be a very effective pretreatment step prior to enzymatic hydrolysis.
Production and Characterization of Phenylalanine Ammonia-lyase from Rhodotorula aurantiaca K-505
Cho, Dae-Haeng,Chae, Hee-Jeong,Kim, Eui-Yong The Korean Society of Food Science and Nutrition 1997 Preventive Nutrition and Food Science Vol.2 No.4
Optimal cultivation conditions for the production of phenylalanine ammonia-lyase(PAL) from Rhodotorula aurantiaca K-505 were selected, and the kinetic parameters of the produced PAL were determined. The most suitable carbon and nitrogen sources were glucose and tryptone, respectively. The strain expressed PAL constituttively when using the optimized semi-complex media. High cell density culture could be critical for maximal production of PAl since the PAL ynthesis was growth associated. maximum PAL activity was observed at initial pH 6.0. although the ll growth was not markedly affected by temperature between 22 and 28$^{\circ}C$, the cells yielded the maximum PAL activity when cultivated at 22$^{\circ}C$. The maximum activity for deamination of L-phenylalnine to trans-cinnamic acid was observed around pH 8.8. The PAL activity gave the maximum at 45$^{\circ}C$, and greatly decreased at higher than 5$0^{\circ}C$. Activation energy({TEX}$E_{a}${/TEX}) calculated from Arrhenius equation was 6.28 kcal/mol in the range of 22$^{\circ}C$ to 4$0^{\circ}C$. A oolf plot showed that the enzyme reaction follows Michaelis-Menten equation, whose {TEX}$K_{M}${/TEX} and {TEX}$V_{max}${/TEX} values were 4.65$\times${TEX}$10^{-3}${/TEX} M and 0.89$\mu$ mol/mg-min respectively.
Production and Characterization of Phenylalanine Ammonia - lyase from Rhodotorula aurantiaca K - 505
Dae-Haeng Cho,Hee-Jeong Chae,Eui Yong Kim 한국식품영양과학회 1997 Preventive Nutrition and Food Science Vol.2 No.4
Optimal cultivation conditions for the production of phenylalanine ammonia-lyase(PAL) from Rhodotorula aurantiaca K-505 were selected, and the kinetic parameters of the produced PAL were determined. The most suitable carbon and nitrogen sources were glucose and tryptone, respectively. The strain expressed PAL constitutively when using the optimized semi-complex media. High cell density culture could be critical for maximal production of PAL since the PAL biosynthesis was growth associated. Maximum PAL activity was observed at initial pH 6.0. Although the cell growth was not markedly affected by temperature between 22 and 28℃, the cells yielded the maximum PAL activity when cultivated at 22℃. The maximum activity for the deamination of L-phenylalanine to trans-cinnamic acid was observed around pH 8.8. The PAL activity gave the maximum at 45℃, and greatly decreased at higher than 50℃. Activation energy(E_a) calculated from Arrhenius equation was 6.28㎉/mol in the range of 22℃ to 40℃. A Hanes-Woolf plot showed that the enzyme reaction follows Michaelis- Menten equation, whose K_M and V_(max) values were 4.65×10^(-3)M and 0.89μ mol/mg-min respectively.