Effects of Nitrogen Concentration on Root Respiration Rate and Nonstructural Carbohydrates of Walnut Seedlings

Chunping Li,Shiwei Wang,Cuifang Zhang,Junjie Ding,Bin Ma,Rabigul Yasin,Zezheng Xue 한국원예학회 2020 원예과학기술지 Vol.38 No.3

Nitrogen is a critical determinant of forest growth and productivity, and root respiration rate and nonstructural carbohydrates are important parameters for evaluating the effects of supplied nutrients. Here, we assessed the effects of nitrogen on walnut seedlings (Juglans regia Linn.) that grew consistently for a year under favorable conditions. Seedlings were potted in seven concentrations of N availability, 0, 1, 2, 4, 8, 12, and 16 mmol·L1, and the specific root respiration rate and nonstructural carbohydrate content of the root system were determined via the offbody root system and the ketone color ratio methods, respectively. Nitrogen concentration was positively correlated with specific root respiration, and root diameter was the main factor related to specific root respiration and nonstructural carbohydrate content of walnut seedlings. Under the same nitrogen concentrations, the soluble total sugar and starch content of roots increased with increasing root diameter, while the specific root respiration increased with decreasing root diameter. Compared to the 0 mmol·L1 nitrogen concentration, the content of nonstructural carbohydrates in the root system and the specific root respiration were higher when nitrogen concentration was N16. Our comprehensive analysis showed that a nitrogen concentration of N16 improved the respiration rate of the seedling root system, significantly increased the content of nonstructural carbohydrates in the seedling root system, and effectively promoted the growth and development of the seedling root system of walnut seedlings.

Universal Indicators for Oil and Gas Prospecting Based on Bacterial Communities Shaped by Light-Hydrocarbon Microseepage in China

( Chunping Deng ),( Xuejian Yu ),( Jinshui Yang ),( Baozhen Li ),( Weilin Sun ),( Hongli Yuan ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6

Light hydrocarbons accumulated in subsurface soil by long-term microseepage could favor the anomalous growth of indigenous hydrocarbon-oxidizing microorganisms, which could be crucial indicators of underlying petroleum reservoirs. Here, Illumina MiSeq sequencing of the 16S rRNA gene was conducted to determine the bacterial community structures in soil samples collected from three typical oil and gas fields at different locations in China. Incubation with n-butane at the laboratory scale was performed to confirm the presence of "universal microbes" in light-hydrocarbon microseepage ecosystems. The results indicated significantly higher bacterial diversity in next-to-well samples compared with background samples at two of the three sites, which were notably different to oil-contaminated environments. Variation partitioning analysis showed that the bacterial community structures above the oil and gas fields at the scale of the present study were shaped mainly by environmental parameters, and geographic location was able to explain only 7.05% of the variation independently. The linear discriminant analysis effect size method revealed that the oil and gas fields significantly favored the growth of Mycobacterium, Flavobacterium, and Pseudomonas, as well as other related bacteria. The relative abundance of Mycobacterium and Pseudomonas increased notably after n-butane cultivation, which highlighted their potential as biomarkers of underlying oil deposits. This work contributes to a broader perspective on the bacterial community structures shaped by long-term light-hydrocarbon microseepage and proposes relatively universal indicators, providing an additional resource for the improvement of microbial prospecting of oil and gas.

전통 몽고 의약으로 사용된 광물 의약의 종류에 관한 고찰

(Li Hongchan),(Yan Shouhe),(Lin Wan),(Guo Chunping),(Wasa Ribete),(King Sheanglong) 자연사.미래환경학회(구.자연사환경학회) 1996 INTERNATIONAL SYMPOSIUM ON NATURAL HISTORY AND ENV Vol.- No.-

Universal Indicators for Oil and Gas Prospecting Based on Bacterial Communities Shaped by Light-Hydrocarbon Microseepage in China

Deng, Chunping,Yu, Xuejian,Yang, Jinshui,Li, Baozhen,Sun, Weilin,Yuan, Hongli The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7

Light hydrocarbons accumulated in subsurface soil by long-term microseepage could favor the anomalous growth of indigenous hydrocarbon-oxidizing microorganisms, which could be crucial indicators of underlying petroleum reservoirs. Here, Illumina MiSeq sequencing of the 16S rRNA gene was conducted to determine the bacterial community structures in soil samples collected from three typical oil and gas fields at different locations in China. Incubation with n-butane at the laboratory scale was performed to confirm the presence of "universal microbes" in light-hydrocarbon microseepage ecosystems. The results indicated significantly higher bacterial diversity in next-to-well samples compared with background samples at two of the three sites, which were notably different to oil-contaminated environments. Variation partitioning analysis showed that the bacterial community structures above the oil and gas fields at the scale of the present study were shaped mainly by environmental parameters, and geographic location was able to explain only 7.05% of the variation independently. The linear discriminant analysis effect size method revealed that the oil and gas fields significantly favored the growth of Mycobacterium, Flavobacterium, and Pseudomonas, as well as other related bacteria. The relative abundance of Mycobacterium and Pseudomonas increased notably after n-butane cultivation, which highlighted their potential as biomarkers of underlying oil deposits. This work contributes to a broader perspective on the bacterial community structures shaped by long-term light-hydrocarbon microseepage and proposes relatively universal indicators, providing an additional resource for the improvement of microbial prospecting of oil and gas.

Follistatin N terminus differentially regulates muscle size and fat in vivo

Hui Zheng,Chunping Qiao,Ruhang Tang,Jianbin Li,Karen Bulaklak,Zhenhua Huang,Chunxia Zhao,Yi Dai,Juan Li,Xiao Xiao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Delivery of follistatin (FST) represents a promising strategy for both muscular dystrophies and diabetes, as FST is a robust antagonist of myostatin and activin, which are critical regulators of skeletal muscle and adipose tissues. FST is a multi-domain protein, and deciphering the function of different domains will facilitate novel designs for FST-based therapy. Our study aims to investigate the role of the N-terminal domain (ND) of FST in regulating muscle and fat mass in vivo. Different FST constructs were created and packaged into the adeno-associated viral vector (AAV). Overexpression of wild-type FST in normal mice greatly increased muscle mass while decreasing fat accumulation, whereas overexpression of an N terminus mutant or N terminusdeleted FST had no effect on muscle mass but moderately decreased fat mass. In contrast, FST-I-I containing the complete N terminus and double domain I without domain II and III had no effect on fat but increased skeletal muscle mass. The effects of different constructs on differentiated C2C12 myotubes were consistent with the in vivo finding. We hypothesized that ND was critical for myostatin blockade, mediating the increase in muscle mass, and was less pivotal for activin binding, which accounts for the decrease in the fat tissue. An in vitro TGF-beta1-responsive reporter assay revealed that FST-I-I and N terminus-mutated or -deleted FST showed differential responses to blockade of activin and myostatin. Our study provided direct in vivo evidence for a role of the ND of FST, shedding light on future potential molecular designs for FST-based gene therapy.

An Automatic Method of Stereoscopic Video Synthesis from Monoscopic Video

Baoliang Wang,Chunping Hou,Xinyu Li,Can Ding 한국산학기술학회 2014 SmartCR Vol.4 No.4

This paper presents a novel depth-image-based rendering system for the generation of stereoscopic video from a monoscopic video sequence. The key point of the proposed system is that all the conversion procedures are accomplished automatically. First, we use Global Motion Estimation and an Adaptive Gaussian Mixture Model to segment the foreground and background. Second, different depth values are assigned to the foreground and background. Finally, image warping according to the depth map and stereoscopic video is synthesized. Experimental results show that the proposed method can effectively segment the foreground from the background and synthesize a satisfactory 3D effect, so the proposed approach is successful.

PREPARATION AND CHARACTERIZATION OF SILVER NANOPARTICLES/CARBON NANOFIBERS VIA ELECTROSPINNING WITH RESEARCH ON THEIR CATALYTIC PROPERTIES

Huan Liu,Jie Bai,Chunping Li,Shan Wang,Yarong Huang,QI WANG,WEIYAN SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.3

Ag nanoparticles (Ag NPs) embedded carbon nano¯bers (CNFs) were prepared by a new routewhich included chemical reaction process, electrospinning and calcination technique. The mor-phology and structure of the composite nano¯bers were investigated by scanning electron mi-croscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy and X-raydi®raction. It indicated that Ag NPs were uniformly distributed in the CNFs. This e®ectivesynthesis method can be used to prepare other composite nano¯bers with functionality. The AgNPs/CNFs that served as supported catalysts were used in the styrene epoxidation by TBHP. The Ag NPs/CNFs catalyst showed its highly catalytic activity for the epoxidation of styrene(conversion: 40.6%, SO selectivity: 35.9%). This kind of composite nano¯ber membrane wasproved e®ectively catalytic activity and recycled easily in the styrene epoxidation.

An Energy-Efficient Multicast Algorithm with Maximum Network Throughput in Multi-hop Wireless Networks

Jiang, Dingde,Xu, Zhengzheng,Li, Wenpan,Yao, Chunping,Lv, Zhihan,Li, Tao The Korea Institute of Information and Commucation 2016 Journal of communications and networks Vol.18 No.5

Energy consumption has become a main problem of sustainable development in communication networks and how to communicate with high energy efficiency is a significant topic that researchers and network operators commonly concern. In this paper, an energy-efficient multicast algorithm in multi-hop wireless networks is proposed aiming at new generation wireless communications. Traditional multi-hop wireless network design only considers either network efficiency or minimum energy consumption of networks, but rarely the maximum energy efficiency of networks. Different from previous methods, the paper targets maximizing energy efficiency of networks. In order to get optimal energy efficiency to build network multicast, our proposed method tries to maximize network throughput and minimize networks' energy consumption by exploiting network coding and sleeping scheme. Simulation results show that the proposed algorithm has better energy efficiency and performance improvements compared with existing methods.

An Energy-Efficient Multicast Algorithm with Maximum Network Throughput in Multi-hop Wireless Networks

Dingde Jiang,Zhengzheng Xu,Wenpan Li,Chunping Yao,Zhihan Lv,Tao Li 한국통신학회 2016 Journal of communications and networks Vol.18 No.5

Energy consumption has become a main problem of sus-tainable development in communication networks and how to com-municate with high energy efficiency is a significant topic that re-searchers and network operators commonly concern. In this paper,an energy-efficient multicast algorithm in multi-hop wireless net-works is proposed aiming at new generation wireless communica-tions. Traditional multi-hop wireless network design only consid-ers either network efficiency or minimum energy consumption ofnetworks, but rarely the maximum energy efficiency of networks. Different from previous methods, the paper targets maximizingenergy efficiency of networks. In order to get optimal energy ef-ficiency to build network multicast, our proposed method triesto maximize network throughput and minimize networks’ energyconsumption by exploiting network coding and sleeping scheme. Simulation results show that the proposed algorithm has better en-ergy efficiency and performance improvements compared with ex-isting methods.

Solvothermal Synthesis of TiO2/CNFs Heterostructures with Photocatalytic Activity

Dandan Yu,Jie Bai,Yulong Gu,Chunping Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.6

Titanium dioxide nanoparticles (TiO2 NPs) embedded carbon nanofibers (CNFs) have been successfully prepared by electrospinning technique, solvothermal synthesis and high-temperature calcination processes. The as-obtained products were characterized by field-emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), UV-Vis diffuse reflectance spectra (DRS) and Fourier transform infrared (FTIR) spectra. The results of characterization revealed that solvothermal treatment and high-temperature calcination were beneficial to the synthesis of anatase TiO2. Compared to the as-fabricated TiO2 powder, TiO2 NPs contained in the TiO2/CNFs were successfully grown on the surface of CNFs without aggregation. The photocatalytic activities of the TiO2/CNFs heterostructures were evaluated by the degradation of methyl orange (MO) under UV light irradiation for 14 h. The TiO2/CNFs exhibited higher photocatalytic activities than pure TiO2 powder, which were attributed to the small size of TiO2 NPs, electron transfer, high adsorption properties of CNFs and the formation of heterostructures between TiO2 and CNFs. Moreover, in aqueous solution, the as-prepared TiO2/CNFs could be easily recycled for the application of CNFs.