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Lee, JeYoung,Li, Changsheng,Surayot, Utoomporn,Yelithao, Khamphone,Lee, SangMin,Park, WooJung,Tabarsa, Mehdi,You, SangGuan Elsevier 2018 International journal of biological macromolecules Vol.120 No.2
<P><B>Abstract</B></P> <P>The water-soluble crude polysaccharides, extracted from the rhizome of <I>Smilax glabra</I>, were fractionated using an anion exchange chromatography, yielding two fractions, F<SUB>1</SUB> and F<SUB>2</SUB>. The crude and fractions (F<SUB>1</SUB> and F<SUB>2</SUB>) mainly consisted of carbohydrates (66.7%–91.1%), proteins (7.30%–23.9%) and minor amount of sulfates (1.60%–9.40%). Glucose was the major monosaccharide unit of the polysaccharides with different levels of sugar constituents including galactose, arabinose, rhamnose and mannose. The molecular weight (M<SUB>w</SUB>) of crude and fractions ranged from 32,102–6.3 × 10<SUP>3</SUP> g/mol. The crude and fractions could stimulate RAW264.7 cells to release nitric oxide and cytokines via up-regulation of their mRNA expression by the activation of NF-κB and MAPKs pathways. The related pattern recognition receptors (PRRs) on the surface of the cells appeared to be TLR2 and CR3. The GC–MS analysis revealed that the main backbone of the most immune-enhancing F<SUB>2</SUB> was (1 → 4)-linked glucose and galactose chain with minor linkages of (1 → 6)-galactose, (1 → 3)-mannose, (1 → 2)-rhamnose and (1 → 5)-arabinose with some branches at C-3 and C-4 rhamnose, or C-6 galactose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Polysaccharide was extracted and purified from <I>Smilax glabra</I> rhizome. </LI> <LI> Two fractions were obtained by DEAE Sepharose fast flow column fractionation. </LI> <LI> The molecular weight of crude and fractions ranged from 32,102 to 6.3 × 10<SUP>3</SUP> g/mol </LI> <LI> The <I>Smilax glabra</I> polysaccharide was appeared enhanced RAW264.7 cells activity. </LI> <LI> The most immune-enhancing F<SUB>2</SUB> polysaccharide mainly consisted of (1 → 4)-linked galactose </LI> </UL> </P>
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Bahramzadeh, Saman,Tabarsa, Mehdi,You, SangGuan,Li, Changsheng,Bita, Seraj Elsevier 2019 Carbohydrate Polymers Vol.205 No.-
<P><B>Abstract</B></P> <P>Sulfated polysaccharides were isolated and purified from the water extract of <I>Cystoseira indica</I> using DEAE Sepharose Fast Flow column to evaluate their structure and macrophage stimulating capacity. Crude and fractionated polysaccharides, CIF<SUB>1</SUB> and CIF<SUB>2</SUB>, were mostly composed of neutral sugars (73.1%–78.6%) with relatively lower amounts of acidic sugars (1.3%–9.0%) and sulfate esters (6.9%–9.7%). The polymer chains of polysaccharides were mainly built of different levels of glucose (2.1%–30.8%), fucose (17.2%–24.4%), mannose (17.8%–20.6%) and galactose (16.7%–17.3%). The weight average molecular weight (<I>M</I> <SUB>w</SUB>) of polysaccharides varied between 573.1 × 10<SUP>3</SUP> g/mol to 1146.6 × 10<SUP>3</SUP> g/mol. The CIF<SUB>2</SUB> polysaccharide, as the most immunostimulating polysaccharide, remarkably induced the release of nitric oxide and inflammatory cytokines including TNF-α, IL-1β, IL-6 and IL-10 from RAW264.7 murine macrophage cells through NF-κB and PAMKs transduction signaling pathways via cell surface TLR4. The interconnections of sugars in CIF<SUB>2</SUB> polysaccharide were complex with (1→3)-fucopyranose, (1→2,3,4)-glucopyranose, (→1)-galactopyranose, (→1)-xylopyranose, (1→2)-rhamnopyranose and (1→2,3)-mannopyranose units being the most predominant residues.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sulfated polysaccharides were isolated from <I>Cystoseira indica.</I> </LI> <LI> Polysaccharides exerted high RAW264.7 macrophage stimulating activity. </LI> <LI> RAW264.7 cells were activated through NF-κB and MAPK signaling pathways. </LI> <LI> TLR4 was the major cell surface receptor interacting polysaccharides with RAW264.7 cells. </LI> <LI> The main backbone of the polysaccharides was consisted of (1→3)-fucopyranosyl units. </LI> </UL> </P>
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Roughness and micro pit defects on surface of SUS 430 stainless steel strip in cold rolling process
Li, Changsheng,Zhu, Tao,Fu, Bo,Li, Youyuan Techno-Press 2015 Advances in materials research Vol.4 No.4
Experiment on roughness and micro pit defects of SUS 430 ferrite stainless steel was investigated in laboratory. The relation between roughness and glossiness with reduction in height, roll surface roughness, emulsion parameters was analyzed. The surface morphology of micro pit defects was observed by SEM, and the effects of micro pit defects on rolling reduction, roll surface roughness, emulsion parameters, lubrication oil in deformation zone and work roll diameter were discussed. With the increasing of reduction ratio strip surface roughness Ra(s), Rp(s) and Rv(s) were decreasing along rolling and width direction, the drop value in rolling direction was faster than that in width direction. The roughness and glossiness were obtained under emulsion concentration 3% and 6%, temperature $55^{\circ}C$ and $63^{\circ}C$, roll surface roughness $Ra(r)=0.5{\mu}m$, $Ra(r)=0.7{\mu}m$ and $Ra(r)=1.0{\mu}m$. The glossiness was declined rapidly when the micro defects ratio was above 23%. With the pass number increasing, the micro pit defects were reduced, uneven peak was decreased and gently along rolling direction. The micro pit defects were increased with the roll surface roughness increase. The defects ratio was declined with larger gradient at pass number 1 to 3, but gentle slope at pass number 4 to 5. When work roll diameter was small, bite angle was increasing, lubrication oil in micro pit of deformation zone was decreased, micro defects were decreased, and glossiness value on the surface of strip was increased.
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Input Disturbance Suppression for Port-controlled Hamiltonian System via the Internal Model Method
Changsheng Li,Yuzhen Wang 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.2
This paper investigates the input disturbance suppression problem for nonlinear Port-Controlled Hamiltonian (PCH) system and presents a number of new results on the controllers design via the internal model approach. Different from the existing results on this topic we consider two cases. Firstly, the case of the disturbance generated from a linear Hamiltonian system acts through a channel other than the input channel is studied by adopting a method of decomposing the control into two parts and designing an internal model to zero the effect of the exogenous disturbance. Secondly, the case of the disturbance generated from a bounded nonlinear exosystem is studied by presenting a procedure of designing a nonlinear internal model to cancel the effect of the disturbance under two fundamental as-sumptions. Moreover, to further improve the suppression, a more effective internal model is also de-signed under less hypotheses. Finally, as an useful application, a corollary is presented by applying these results on PCH system to general nonlinear affine system. Simulations of a third-order synchro-nous generator model with disturbance generated from a nonlinear exosystem show the effectiveness of the designed internal model.
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Changsheng Zhu,Mingfang Zhu,Jieqiong Liu,Li Feng 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.10
By coupling the phase field model of dendrite growth with the Lattice Boltzmann method (LBM), a Phase field LBM model (PFLBM) that can simulate 3D dendrite non-isothermal growing from the under-cooled melt of a pure substance in forced flow has been established. Taking high-purity succinonitrile (SCN) as an example, the effects of flow on 3D dendritic growth were studied. The calculation efficiency of LBM and SOLA algorithm was compared under the same condition. The results show that, with the PF-LBM model, the effects of forced flow on 3D dendrite growth morphology and the dendrite asymmetric growth can be quantitatively modeled in large scale, and the dendrite growth is consistent with the crystallization theory. The LBM algorithm overcomes the weakness of a large amount of calculation and slow computation speed that exists in the traditional algorithm, and it achieves a speedup of 11.7 when the calculation region size is 200 3 .
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Hongming Li,Yumei Qiu,Mengdie Xie,Changsheng Ouyang,Xiaoyun Ding,Hao Zhang,Wei Dong,Yinhua Xiong,Xilan Tang 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1
This study aimed to observe the protective effect of momordicine I, a triterpenoid compound extracted from momordica charantia L., on isoproterenol (ISO)-induced hypertrophy in rat H9c2 cardiomyocytes and investigate its potential mechanism. Treatment with 10 μM ISO induced cardiomyocyte hypertrophy as evidenced by increased cell surface area and protein content as well as pronounced upregulation of fetal genes including atrial natriuretic peptide, β-myosin heavy chain, and α-skeletal actin; however, those responses were markedly attenuated by treatment with 12.5 μg/ml momordicine I. Transcriptome experiment results showed that there were 381 and 447 differentially expressed genes expressed in comparisons of model/control and momordicine I intervention/model, respectively. GO enrichment analysis suggested that the anti-cardiomyocyte hypertrophic effect of momordicine I may be mainly associated with the regulation of metabolic processes. Based on our transcriptome experiment results as well as literature reports, we selected glycerophospholipid metabolizing enzymes group VI phospholipase A2 (PLA2G6) and diacylglycerol kinase ζ (DGK-ζ) as targets to further explore the potential mechanism through which momordicine I inhibited ISO-induced cardiomyocyte hypertrophy. Our results demonstrated that momordicine I inhibited ISO-induced upregulations of mRNA levels and protein expressions of PLA2G6 and DGK-ζ. Collectively, momordicine I alleviated ISO-induced cardiomyocyte hypertrophy, which may be related to its inhibition of the expression of glycerophospholipid metabolizing enzymes PLA2G6 and DGK-ζ.
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Bo Li,BaoXing Bai,Changsheng Zhang,Yixue Jiang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.8
Prediction of network public opinion is a complicated prediction featuring poor information, small samples and uncertainty. A prediction model of network public opinion based on grey support vector machine (SVM) is specified to increase prediction accuracy. First, network data are preprocessed by text clustering, hotpot extraction and data aggregation. Then a time series model GM(1,1) is established and SVM is used to modify prediction outcomes of GM(1,1). At last, simulation experiment is conducted to test performance of the model. Simulation results indicate that grey SVM improves the prediction accuracy of network public opinion compared with traditional prediction models. The predictions have certain practical values.
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Characterization of Hot Deformation Behavior and Processing Map of As‑Cast H13 Hot Work Die Steel
Yahui Han,Changsheng Li,Jinyi Ren,Chunlin Qiu,En LI,Shuaishuai Chen 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9
The hot-working behavior of as-cast H13 hot work die steel was investigated in the method of isothermal compression testinvolving the wide deformation temperatures of 900–1150 °C and strain rates of 0.01–10 s−1, with the true strain to 0.8, onthe MMS-200 thermo-mechanical simulator. Two characteristic parameters involving the critical strain for DRX initiation(c ) and the strain for peak stress (p ) were identified. The ratio of critical strain to peak strain ranged from 0.26 to 0.6, whichdecreased with the increase in temperature and decrease in strain rate. Processing maps were established using dynamicmaterial model at strains of 0.2, 0.4, 0.6, 0.8. The power dissipation maps were not significantly affected by the strain, whilethe instability maps were sensitivity with the strain when it was over 0.4. The area of instability domain at strain of 0.8 wasthe largest. The instable characteristics contained the mixed grain structure, adiabatic shear band, intense deformation inserious deformation area and brittle elemental segregation area. The chief effect on the power dissipation was the strain rate,the optimum hot working parameters at strain of 0.8 (910–985 °C, 1010–1150 °C and 0.01–0.05 s−1) were determined. Inthis filed, the original coarse as-cast grains were gradually refined by dynamic recrystallization mechanism and the DRXgrain numbers had a significant increase with the increase of power dissipation efficiency.
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