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Evaluation of Cartilage Regeneration in Gellan Gum/agar Blended Hydrogel with Improved Injectability
백종선,Cristiano Carlomagno,Thangavelu Muthukumar,김다윗,박종호,송정은,Claudio Migliaresi,Antonella Motta,Rui L. Reis,강길선 한국고분자학회 2019 Macromolecular Research Vol.27 No.6
Gellan gum is known to be one of the biocompatible materials that resemble living tissues, continuously delivering stem cells or general cells, and filling voids in the wound. In spite of the many advantages of gellan gum, it has difficulty in the application by inserting the scaffolds into the body. In order to generalize gellan gum for medical purpose, it is necessary to increase the injectability of gellan gum. In this study, gellan gum solutions with 0.2, 0.4, 0.6, 0.8 wt% of agar were prepared. The obtained hydrogels were tested for rheological properties and chondrocytes adhesion, morphology and proliferation. Results showed that the addition of agar, in the starting GG solution, allows extruding the material producing micro-porous scaffolds. The biological evaluation demonstrated that the adhesion and proliferation of chondrocytes increase with the presence of agar in the final structure. The solutions composed of GG and agar represent an efficient, fast and high reproducible methodology with the potential to be used as an injectable hydrogel for cartilage regeneration purposes.
RET: A Multi-Faceted Gene in Human Cancer
Massimo Santoro,Francesca Carlomagno,Rosa Marina Melillo 대한내분비학회 2012 Endocrinology and metabolism Vol.27 No.3
REarranged during Transfection (RET) gene encodes a receptor tyrosine kinase and it was initially discovered as an in vitro transforming gene. For many years, RET has been involved in papillary thyroid carcinoma and medullary thyroid carcinoma. More recently,lung adenocarcinoma and chronic myelomonocytic leukemia samples have been found to display RET gene rearrangements. This knowledge is stimulating the search for protein kinase inhibitors to combat RET-driven malignancies.
신은영,박종호,신명은,송정은,Cristiano Carlomagno,강길선 한국고분자학회 2019 Macromolecular Research Vol.27 No.4
The poor proliferative ability of chondrocytes makes complicated the cartilage regeneration after injuries or during the pathological state. Nowadays, stem cells represent a potential tool for different tissues regeneration, including cartilage. Previous studies demonstrated the role of miRNAs (MicroRNAs) in chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through the in inhibition of specific genes expression. In this study, miR-30a was used to assess the possible chondrogenic differentiation, in combination with silk fibroin/gellan gum (SF/GG) hydrogels, suitable for cells sustaining and proliferation. The SF/GG hydrogel was fabricated combining 2% of gellan gum with 2% of silk fibroin, exploiting the cationic cross-linking of the polysaccharide. For characterization, the hydrogel was lyophilized and used. Scanning electron microscopy was used to characterize the scaffold morphology, while FT-IR spectroscopy was performed to evaluate the chemical properties. Suitability of the produced scaffold for cells adhesion and nutrient and oxygen perfusion was evaluated through water uptake and overall porosity. BMSCs extracted from rats were transfected with miR-30a mimic and inhibitor. MiR-30a expression rates were measured by real time-quantitative polymerase chain reaction (qPCR) monitoring the expression of cartilage-specific gene through reverse transcription-polymerase chain reaction (RT-PCR). Histological assays were used to identify the chondrogenesis of BMSCs on the SF/GG hydrogel. Our results demonstrated the suitability of the SF/GG hydrolgel for cells adhesion, ingrowth and nutrients perfusion. The exposition of cells to the miR-30a demonstrated the potential role of the molecule in chondrogenic differentiation showing an up regulation of cartilage-specific gene. In conclusion, stem cells transfected with miRNA can positively affect articular cartilage regeneration and the potential of BMSCs-encapsulated hydrogel transfected with miR-30a as a therapeutics for osteoarthritis (OA) has been confirmed.
Park, Jong Ho,Shin, Eun Yeong,Shin, Myeong Eun,Choi, Min Joung,Carlomagno, Cristiano,Song, Jeong Eun,Khang, Gilson Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.117 No.-
<P><B>Abstract</B></P> <P>The retinal pigment epithelium (RPE) plays a significant role in retaining structural integrity of eye. Factors such as reduction in cell regeneration due to aging and physical injury pose a major hurdle in RPE regeneration. In this study, we exploited the use of alginate (AGT) incorporated with Curcumin (CCI) forming a hydrogel based system CCI/AGT. The fabricated hydrogel could anchor RPE cell in it. <I>In vitro</I> cell analysis revealed that the CCI/AGT hydrogel shows good biocompatibility, enhanced cell growth ability and higher ECM formation compared to the pure AGT hydrogel. In particular, the presence of CCI in the hydrogels enhances the cells proliferation of the 23% respect to the pure alginate. Also the expression of crucial genes for retina functions and matrix production were positively affected by CCI presence, with an increment of 45% for RPE65, 32% for CRALBP and 26% for Collagen type 1. <I>In vitro</I> tests demonstrated the potential application of CCI/AGT hydrogels for transplantation under the sub-retinal space acting as a cell delivery vehicle and also their capability to provide an appropriate environment for RPE regeneration. These results suggest that CCI/AGT hydrogel could be translated into a potential surgical graft for biological implantation of retinal tissue engineering.</P>