Down-regulation of SENP1 Expression Increases Apoptosis of Burkitt Lymphoma Cells

Huang, Bin-Bin,Gao, Qing-Mei,Liang, Wei,Xiu, Bing,Zhang, Wen-Jun,Liang, Ai-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To investigate the effect of down-regulation of Sentrin/SUMO-specific protease 1 (SENP1) expression on the apoptosis of human Burkitt lymphoma cells (Daudi cells) and potential mechanisms. Methods: Short hairpin RNA (shRNA) targeting SENP1 was designed and synthesized and then cloned into a lentiviral vector. A lentiviral packaging plasmid was used to transfect Daudi cells (sh-SENP1-Daudi group). Daudi cells without transfection (Daudi group) and Daudi cells transfected with blank plasmid (sh-NC-Daudi group) served as control groups. Flow cytometry was performed to screen GFP positive cells and semiquantitative PCR and Western blot assays were employed to detect the inference efficiency. The morphology of cells was observed under a microscope before and after transfection. Fluorescence quantitative PCR and Western blot assays were conducted to measure the mRNA and protein expression of apoptosis related molecules (caspase-3, 8 and 9). After treatment with $COCl_2$ for 24 h, the mRNA and protein expression of hypoxia inducible factor -$1{\alpha}$ (HIF-$1{\alpha}$) was determined. Results: Sequencing showed the expression vectors of shRNA targeting SENP1 to be successfully constructed. Following screening of GFP positive cells by FCM, semiqualitative PCR showed the interference efficiency was $79.2{\pm}0.026%$. At 48 h after transfection, the Daudi cells became shrunken, had irregular edges and presented apoptotic bodies. Western blot assay revealed increase in expression of caspase-3, 8 and 9 with prolongation of transfection (P<0.05). Following hypoxia treatment, mRNA expression of HIF-$1{\alpha}$ remained unchanged in three groups (P>0.05) but the protein expression of HIF-$1{\alpha}$ markedly increased (P<0.05). However, in the sh-SENP1-Daudi group, the protein expression of HIF-$1{\alpha}$ remained unchanged Conclusion: SENP1-shRNA can efficiently inhibit SENP1 expression in Daudi cells. SENP1 inhibition may promote cell apoptosis. These findings suggest that SENP1 may serve as an important target in the gene therapy of Burkitts lymphoma.

A Monocular Structured Light Vision Method for Pose Determination of Large Non-cooperative Satellites

Bin Liang,Xue-Hai Gao,Le Pan,Zhi-Heng Li,Ying-Chun Zhang 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.6

A space robotic system is expected to perform on-orbit servicing missions to rescue malfunctionedsatellites in geostationary orbit (GEO). In final berthing and capture, it is difficult for a space robot to determine therelative pose (attitude and position) of a non-cooperative malfunctioned satellite that is usually huge and withoutartificial recognition devices. In this paper, a space robot with a monocular structured light vision subsystem isintroduced to solve the problem. Firstly, the monocular structured light vision subsystem composed of a singlecamera and a point light source is designed. Secondly, a partial rectangular shaped framework, which is verycommon on a non-cooperative malfunctioned satellite, is chosen as the recognition object for non-cooperative posemeasurement. Using projection constraints on rectangle and circular points, a rectangle feature reconstructionalgorithm is proposed. Thirdly, according to the reconstructed rectangle feature, a least square method of posedetermination is presented. Lastly, using a semi-physical vision simulation system, several experiments of typicalcases are simulated to verify the pose determination method of large non-cooperative target. The results show thevalidity and flexibility of the proposed method.

Mechanical behavior of steel tube encased high-strength concrete composite walls under constant axial load and cyclically increasing lateral load: Experimental investigation and modeling

Liang Bai,Huilin Wei,Bin Wang,Fangfang Liao,Tianhua Zhou,Xing-Wen Liang 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.1

This paper presented an investigation into steel tubes encased high-strength concrete (STHC) composite walls, wherein steel tubes were embedded at the boundary elements of high-strength concrete walls. A series of cyclic loading tests was conducted to evaluate the failure pattern, hysteresis characteristics, load-bearing capacity, deformability, and strain distribution of STHC composite walls. The test results demonstrated that the bearing capacity and ductility of the STHC composite walls improved with the embedding of steel tubes at the boundary elements. An analytical method was then established to predict the flexural bearing capacity of the STHC composite walls, and the calculated results agreed well with the experimental values, with errors of less than 10%. Finally, a finite element modeling (FEM) was developed via the OpenSees program to analyze the mechanical performance of the STHC composite wall. The FEM was validated through test results; additionally, the influences of the axial load ratio, steel tube strength, and shear-span ratio on the mechanical properties of STHC composite walls were comprehensively investigated.

Low Temperature Cure Film Adhesive

Liang, Bin,Zhao, Shenglong The Society of Adhesion and Interface 2004 접착 및 계면 Vol.5 No.2

A novel carboxyl terminated butadiene-acrylonitrile (CTBN) modified, low temperature cure epoxy film adhesive was developed in this paper. It can be cured at as low as $75^{\circ}C$ for 4 hours with a pressure of 0.1MPa. After post cure at $120^{\circ}C$ for 2 hours, the bonding strengths of Phosphoric Acid Anodizing(PAA) surface treated aluminum adherend were similar to those of structural film adhesives curing at $120^{\circ}C$. It is suitable to bond both metal/composite laminate-to-laminate and laminate to honeycomb structure.

Low Temperature Cure Film Adhesive

( Bin Liang ),( Sheng Long Zhao ) 한국 접착 및 계면학회 2004 접착 및 계면 Vol.5 No.2

Compound glycyrrhizin injection for improving liver function in children with acute icteric hepatitis: A systematic review and meta-analysis

Liang Shi-Bing,Hou Wen-Bin,Zheng Ruo-Xiang,Liang Chang-Hao,Yan Li-Jiao,Wang Hao-Nan,Cao Hui-Juan,Han Mei,Robinson Nicola,Liu Jian-Ping 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.1

Background: Compound glycyrrhizin injection (CGI) is a preparation with glycyrrhizin as the main active ingredient extracted from licorice. As clinical trials suggest that CGI is effective in improving liver function for acute icteric hepatitis in children (AIHC), this systematic review aimed to evaluate and verify its therapeutic effects and safety. Methods: Six electronic databases were searched from their inception to 15 May 2021. Randomized controlled trials (RCTs) assessing therapeutic effects and safety of CGI for AIHC were included. The risk of bias for each trial was assessed using the Cochrane Risk of Bias Tool 2.0. Primary outcomes were indexes related to liver function, including total bilirubin (TBiL), alanine aminotransferase (ALT) and aspartate transaminase (AST). RevMan 5.4 software was used for data analyses. The certainty of the evidence was assessed using the online GRADEpro tool. Results: Six RCTs involving 608 children were included. The overall bias was assessed as having “high risk of bias” in all trials. All trials compared the combination of CGI and conventional western medicine (CWM) with CWM alone. Regarding the effects of CGI for AIHC, results showed that CGI plus CWM was superior to CWM alone in reducing the levels of TBiL (mean difference (MD) = -8.19 mmol/L, 95% CI -9.86 to -6.53), ALT (MD = -24.09 U/L, 95% CI -30.83 to -17.34) and AST (MD = -18.67 U/L, 95% CI -21.88 to -15.45). No trial reported adverse events. The certainty of the evidence for outcomes were all evaluated as low or very low. Conclusion: CGI may have adjuvant therapeutic effects on improving the liver function of children with AIHC. There is no evidence to determine the safety of CGI for AIHC. As current evidence is weak, further well-designed RCTs are required for verification of the therapeutic effects of CGI. Background: Compound glycyrrhizin injection (CGI) is a preparation with glycyrrhizin as the main active ingredient extracted from licorice. As clinical trials suggest that CGI is effective in improving liver function for acute icteric hepatitis in children (AIHC), this systematic review aimed to evaluate and verify its therapeutic effects and safety. Methods: Six electronic databases were searched from their inception to 15 May 2021. Randomized controlled trials (RCTs) assessing therapeutic effects and safety of CGI for AIHC were included. The risk of bias for each trial was assessed using the Cochrane Risk of Bias Tool 2.0. Primary outcomes were indexes related to liver function, including total bilirubin (TBiL), alanine aminotransferase (ALT) and aspartate transaminase (AST). RevMan 5.4 software was used for data analyses. The certainty of the evidence was assessed using the online GRADEpro tool. Results: Six RCTs involving 608 children were included. The overall bias was assessed as having “high risk of bias” in all trials. All trials compared the combination of CGI and conventional western medicine (CWM) with CWM alone. Regarding the effects of CGI for AIHC, results showed that CGI plus CWM was superior to CWM alone in reducing the levels of TBiL (mean difference (MD) = -8.19 mmol/L, 95% CI -9.86 to -6.53), ALT (MD = -24.09 U/L, 95% CI -30.83 to -17.34) and AST (MD = -18.67 U/L, 95% CI -21.88 to -15.45). No trial reported adverse events. The certainty of the evidence for outcomes were all evaluated as low or very low. Conclusion: CGI may have adjuvant therapeutic effects on improving the liver function of children with AIHC. There is no evidence to determine the safety of CGI for AIHC. As current evidence is weak, further well-designed RCTs are required for verification of the therapeutic effects of CGI.

연변 지역의 헤드카피 언어 사용에 반영된 언어태도 연구

량빈(Liang, Bin) 한국사회언어학회 2018 사회언어학 Vol.26 No.1

The purpose of this article is to study the language attitudes toward Yanbian, Korean, and Chinese through the use of head - copy language in the Yanbian area. The Yanbian bilingual speaker mixes the dialects of North Hamgyong, Korean, and Chinese. In order to analyze the use of language in the Yanbian area, it is necessary to accurately understand the language attitudes of Yanbian, Korean, and Chinese. ‘Language attitude’ is not only the evaluation and response of a speaker to a specific language, but a concrete action. The research on the language use patterns of the bilingual speakers in the Yanbian area can analyze the language attitudes for each language. Advertising language includes not only the function of information transmission but also cultural awareness of society. The attitude of language, which is one of the human consciousness forms, is also included in the advertising language and the attitude of language can be analyzed through the use of advertising language. In this paper, I will proceed with the SPSS chi-square test based on the usage of the head copy in the Yanbian area. If there is statistical significance, I will describe what kind of language attitude is displayed for each language.

연변 지역어에서의 대화 요청 행위 표현에 대한 고찰

梁彬(Liang, Bin),趙宰亨(Cho, Chae-hyung) 중앙어문학회 2017 語文論集 Vol.69 No.-

이 글의 목적은 연변 지역어의 대화 요청 행위 표현을 Wierzbicka(2003)에서 記述한 ‘자연 의미 메타언어(Nature Semantic Metalanguage)’ 를 이용하여 고찰하는 데에 있다. 연변 지역어는 ‘바일링구얼리즘(bilingualism)’의 특성을 가지고 있다. 따라서 의미적 특성을 분석하려면 어느 한 언어의 문화 요소만으로 접근하기보다는 함경북도 방언, 중국어, 한국어가 가지고 있는 복합적인 문화요소를 고려해야 한다. 이러한 문제를 해결하기 위하여 ‘자연 의미 메타언어(Nature Semantic Meta-language)’ 방법론을 이용하였다. NSM 방법론은 모든 문화에 자연적으로 내재되어 있는 ‘의미 원초소(semantic primitives)’를 이용하여 표현을 메타 언어적으로 기술하는 것이다. 설문조사를 통하여 사용 빈도가 가장 높은 4개의 표현 ‘허드레 치다’, ‘말 하자’, ‘커피를 마시자’, ‘까페에서 보자’ 를 추출하였다. 추출한 표현들이 가지고 있는 문화적 자질을 근거로 메타언어적으로 기술하였고 나아가 여러 표현들 사이의 의미적 차이를 기술하였다. In this paper, we use Wierzbicka’s (2003) natural semantic metalanguage (NSM) to investigate request dialogs in the local Yanbian language. Speakers of Yanbian are all multilingual . Therefore, in order to analyze the semantic characteristics of these dialogs, it is necessary to consider the complex cultural interaction of Yanbian , Chinese, and Korean in North Hamkyong province, rather than approach the dialogs only with cultural elements of a single language. We used NSM to carry out this work. NSM methodology metalinguistically describes the expression of semantic primitives, which are held to be inherent in all cultures. Using questionnaires, we extracted the four most frequently used expressions: /hʌ ð rɛ tʃi dɑ/ (허드레 치다, /mɑl ha zɑ/ (말 하자), /kʌ fi rl mɑ si zɑ/(커피를 마시자), and /kʼɑ fe ŋe sə vɔ zɑ/ (까페에서 보자). Based on the cultural qualities of the expressions studied, we describe them metalinguistically and analyze their semantic differences.

Effect of different alfalfa cultivars on growth and development of the spotted alfalfa aphid, Therioaphis trifolii(Monell)

Yu Liang‐bin,Lin Ke‐Jian,Xu Lin‐bo,Wang Hui,Cui Jin,Zhang Quan‐yi,Wang Ya‐ping,Yan Li‐ying 한국곤충학회 2022 Entomological Research Vol.52 No.3

Therioaphis trifolii (Monell) is a well-known pest in alfalfa cultivation in China. Individuals of T. t r i f o l i i were allowed to feed on 10 alfalfa cultivars and their growth, reproduction, and population life table parameters were estimated. Nymphs that fed on Hunter River had the longest development time, whereas nymphs that fed on Defu had the shortest development time. Adults that fed on Debao, Zhongmu No. 2, and Defu had the shortest development time, whereas adults that fed on Golden Empress and Hunter River had the longest development times. Individuals that fed on Zhungeer produced the greatest number of offspring (13.950), and those that fed on Hunter River produced the fewest offspring (6.000). The survival rate of T. t r i f o l i i was the highest on Algonquin and lowest on Hunter River, whereas the survival time was the longest on Golden Empress and shortest on Debao. The population that fed on Algonquin had the highest intrinsic growth rate (0.560) and shortest population doubling time (1.237 days), whereas those that fed on Hunter River had the lowest intrinsic growth rate (0.217) and longest population doubling time (3.194 days). The life table parameters of the experimental T. t r i f o l i i population were used to quantify the resistance strengths of the cultivars. Results indicate that the Hunter River cultivar may effectively resist damage from T. trifolii. This study provides a theoretical basis for breeding aphid-resistant alfalfa cultivars, enabling further research into the prevention and control of aphid infestation.

Activating Transcription Factor 1 is a Prognostic Marker of Colorectal Cancer

Huang, Guo-Liang,Guo, Hong-Qiang,Yang, Feng,Liu, Ou-Fei,Li, Bin-Bin,Liu, Xing-Yan,Lu, Yan,He, Zhi-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Objective: Identifying cancer-related genes or proteins is critical in preventing and controlling colorectal cancer (CRC). This study was to investigate the clinicopathological and prognostic value of activating transcription factor 1 (ATF1) in CRC. Methods: Protein expression of ATF1 was detected using immunohistochemistry in 66 CRC tissues. Clinicopathological association of ATF1 in CRC was analyzed with chi-square test or Fisher's exact test. The prognostic value of ATF1 in CRC is estimated using the Kaplan-Meier analysis and Cox regression models. Results: The ATF1 protein expression was significantly lower in tumor tissues than corresponding normal tissues (51.5% and 71.1%, respectively, P = 0.038). No correlation was found between ATF1 expression and the investigated clinicopathological parameters, including gender, age, depth of invasion, lymph node status, metastasis, pathological stage, vascular tumoral emboli, peritumoral deposits, chemotherapy and original tumor site (all with P > 0.05). Patients with higher ATF1 expression levels have a significantly higher survival rate than that with lower expression (P = 0.026 for overall survival, P = 0.008 for progress free survival). Multivariate Cox regression model revealed that ATF1 expression and depth of invasion were the predictors of the overall survival (P = 0.008 and P = 0.028) and progress free survival (P = 0.002 and P = 0.005) in CRC. Conclusions: Higher ATF1 expression is a predictor of a favorable outcome for the overall survival and progress free survival in CRC.