Immunobiotics Beneficially Modulate TLR4 Signaling Triggered by Lipopolysaccharide and Reduce Hepatic Steatosis In Vitro

Kanmani, Paulraj,Ansari, AbuZar,Villena, Julio,Kim, Hojun Hindawi 2019 Journal of immunology research Vol.2019 No.-

<P>Hepatic inflammation and injury may result from the translocation of pathological bacteria and their proinflammatory mediators. Probiotics attenuate hepatic diseases related to inflammation by exhibiting immunoregulatory effects. Therefore, this study was conducted to evaluate lipid reduction and immunoregulatory potentials of probiotic bacteria in vitro. HepG2 cells treated with total cellular fluid (TCF) of LABs reduced lipid accumulation. Moreover, cells responded to lipopolysaccharide (LPS) by producing higher levels of IL-6, IL-8, MCP-1, and TNF-<I>α</I>. TCF of LABs treatment showed remarkably diminished levels of the expression of these cytokines via modulation of the expression of TLR-negative regulators, as well as MAPK and NF-<I>κ</I>B pathways. Moreover, heat-killed LABs were able to diminish TGF-<I>β</I>, IL-1<I>β</I>, and IL-6 and to increase IL-10 and TLR4 levels in THP-1 cells. LABs also decreased the protein level of TNF-<I>α</I>. These results demonstrated that immunobiotics exhibit potent immunoregulatory activity and may be used as effective therapeutic agents to alleviate inflammatory response.</P>

Lactobacillus probiotics improves dysbiotic vaginal microbiota through gut-vagina-axis in Korean women

( Sunwha Park ),( Abuzar Ansari ),( Doo Heon Son ),( Young Min Hur ),( Young-ah You ),( Soo-min Kim ),( Gain Lee ),( Seungbeom Kang ),( Sang Hyeon Lim ),( Young-ju Kim ) 대한산부인과학회 2022 대한산부인과학회 학술대회 Vol.108 No.-

Objective: The objective of the study is to determine whether Lactobacillus probiotics improves dysbiotic vaginal microbiota through gut-vagina-axis in Korean women. Methods: This clinical trial was conducted on 57 women who visited at Ewha Womans University Hospital in 2021 for routine gynecological examination. We gave probiotics for total 6 weeks to subjects and their cervicovaginal fluid (CVF) samples were collected with 3 times (before, after 3, and 6 weeks from taking probiotics). The Lactobacillus probiotics were contained of L. reuteri, L. rhamnosus, and L. acidophilus. We checked STD PCR and gram stain for calculating Nugent score and analyzed microbiome by NGS. Raw sequencing data were processed by 16s rRNA sequencing using Quantitative Insight into Microbial Ecology software package 2 (QIIME 2, v2021.11). Data visualization was performed using the ggplot2 package of R (v4.1.3), and statistical analysis was performed through Wilcoxon Rank-Sum Test, Kruskal-Wallis Rank Sum Test and PERMANOVA using the vegan package. P values <0.05 were considered statistically significant. Results: The subjects with normal group were 26 women and the abnormal group was 10 women according to the Nugent score and NGS analysis. The 26 women were represented of eubiosis state that Lactobacillus accounted for more than 90% and the 10 women showed dysbiosis state with high alpha-diversity according to NGS. There was a significant correlation between Nugent score and alpha-diversity (R=0.37 for OTUs, p<0.001; R=0.45 for Shannon diversity, p<0.001; R=0.41 for Evenness, p<0.001, respectively). Among the abnormal group, 60% of women was significantly changed to normal vaginal environment after taking Lactobacillus probiotics by oral route for 6 weeks (p<0.05). After taking probiotics, their alpha-diversity was decreased and beta-diversity was improved to the normal condition. Conclusion: This study suggests that Lactobacillus probiotics by oral route could improve the vaginal environment and change the microbiome composition.

Prediction of preterm birth using bacteria risk score

( Sunwha Park ),( Soo Min Kim ),( Abuzar Ansari ),( Young-ah You ),( Daejoong Oh ),( Myunghoon Lee ),( Yun Ji Jung ),( Young-han Kim ),( Young Ju Kim ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: The objective of the study was to characterize bacteria profiles in cervicovaginal fluid (CVF), and determine their predictive values for preterm birth (PTB) prediction. Methods: A multi-center prospective cohort study (Ewha Womans University, Mokdong Hospital, Severance Hospital, Yonsei University College of Medicine) consisting of 94 pregnant women with PTBs (n=39) and term births (TB; n=55) from the period of 2018 to 2019 were investigated. Clinical characteristics, blood test results, cervical length (CL), and CVF samples were collected in the second trimester. Qualitative/quantitative analysis of eight bacteria (L. crispatus, W. koreensis, B. fragilis, P. bivia, P. amnii, P. salivae, U. urealyticum, U. Pavum) were done using PCR. After making a normal delivery/preterm division model, we checked cross-validation performance (Support Vector Machine, 3rd order polynomial). R software was used for our statistical analyses and p values < 0.05 were considered statistically significant. The prediction model was generated using machine learning techniques (Random forest). Results: Each individual bacterium (L. crispatus, W. koreensis, B. fragilis, P. bivia, P. amnii, P. salivae, U. urealyticum, U. Pavum) had no statistically significant association with preterm birth (p value >0.05). Association with preterm birth was significantly confirmed in 14 bacterial combinations. (p value<0.05) Among them, the combination of B. fragilis, P. bivia, and U. pavum had the highest statistical significance in our analysis. A bacteria risk score model using these three bacteria combinations to predict PTB was statistically significant (p value<0.05, test AUC 0.646). Conclusion: We concluded that a risk score model with multiple bacterial combinations could be a significant predictor of PTB.

Can probiotics regulate programmed obesity induced by maternal diet?

( Bo Ra Kim ),( Sunwha Park ),( Soo Min Kim ),( Abuzar Ansari ),( Young-ah You ),( Young Ju Kim ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: This study investigated the effects of Weissella confusa, WIKIM51 on the programmed obesity induced by maternal diet. Methods: Pregnant rats were divided into four groups: 1) AdLib/AdLib (control; given a normal diet during the experimental period), 2) FR/AdLib (given a food restriction diet during pregnancy and a normal diet during lactation), 3) HF/AdLib (given a high fat diet during pregnancy and a normal diet during lactation), 4) HF/HF (given a high fat diet during the experimental period). In each group, 10-week-old offspring were randomly divided into 2 groups and fed WIKIM51 for 6 weeks. We measured the body weight and dietary intake of the offspring, and analysed lipid profiles. The liver and lipid droplets were observed by histopathological analysis. The mRNA and protein levels of hepatic lipid metabolism-related genes were measured by real-time PCR and a western blot. Statistical analysis were performed using SPSS ver. 21. Results: Birth weight significantly decreased among FR offspring compared to the other 3 groups. At 10 weeks of age, the weight of the FR offspring significantly decreased compared to the weight of the control group, while the weight of the obese offspring significantly increased. After taking WIKIM51, body weight at 16-weeks significantly decreased in the FR and HF group compared to those that did not take WIKIM51 (P<0.05). In addition, weight gain significantly decreased in all groups compared to those that did not take WIKIM51 (P<0.05). Blood glucose levels of FR offspring consuming WIKIM51 also decreased, as did blood triglyceride levels of obese offspring consuming WIKIM51. Furthermore, hepatic cell size decreased in all offspring consuming WIKIM51 compared to those that did not take WIKIM51. The expression and protein levels of hepatic lipid metabolism related genes were altered in all offspring consuming WIKIM51. Conclusion: Our findings suggest that WIKIM51 intake will help to recover the adverse health outcomes of offspring induced by maternal diet.

The introduction to air pollution on pregnancy outcome (APPO) study: a multicenter cohort study

( Young Min Hur ),( Sunwha Park ),( Eunjin Kwon ),( Young-ah You ),( Abuzar Ansari ),( Soo Min Kim ),( Gain Lee ),( Mi Hye Park ),( Kyung A Lee ),( Soo Jung Kim ),( Young-han Kim ),( Yeon-sung Jung ) 대한산부인과학회 2023 Obstetrics & Gynecology Science Vol.66 No.3

Objective The air pollution on pregnancy outcome (APPO) study is a prospective hospital-based cohort study designed to investigate the maternal and fetal effects of a particulate matter with an aerodynamic below 10 μm (PM10) and PM2.5 (below 2.5 μm) exposure. This study aims to analyze a relationship between particulate matter and adverse pregnancy outcomes and to find related biomarkers and develop management guidelines. Methods About 1,200 pregnant women are recruited for 3 years (from January 2021 to December 2023) from seven university hospitals to investigate the effects of particulate matter on pregnancy complications and adverse pregnancy outcomes. We collect biological samples by 5 mL of maternal venous blood and 15 mL of urine in each trimester of pregnancy, and 5 mL of umbilical cord blood and 2×2×2 cm of placental tissue are collected after delivery. In addition, by applying PM10 and PM2.5 concentration values and time-activity patterns from the time weighted average model, the individual predicted exposure of air pollution for the pregnant women are obtained. Results The average exposure of PM10 and PM2.5 of the participants in the entire period of pregnancy, was exceeded the World Health Organization air quality guidelines (an annual level, PM10 >15 μg/m³, PM2.5 >5 μg/m³). Moreover, it was revealed that the PM concentration was increasing toward the 3rd trimester of pregnancy. Conclusion The APPO study will be able to identify the degree of exposure to air pollution in pregnant women and use it as basic data for estimating individual exposure to particulate matter. And the results of the APPO study will facilitate in the development of health management for pregnant women against air pollution.

Ephedra Sinica Diversified Intestinal Microbiota Ameliorates High Fat Diet induced Obesity in Sprague Dawley Rats

Jing-Hua Wang,Ahtsham Hussain,Kyung-Sun Han,Soo-kyoung Lim,Narae Shin,Dongwoo Lim,Abuzar Ansari,Hojun Kim 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Heightened anti-oxidative and anti-inflammatory activity of selective lactobacillus starter derived from Samjunghwan

Narae Shin,Jing-Hua Wang,Ahtsham Hussain,Kyung-Sun Han,Soo-kyeong Lim,Dongwoo Lim,Abuzar Ansari,Hojun Kim 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8