각종 작물로 부터 분리한 Fusarium 속 균의 RAPD 기법을 이용한 유전분석

최혜선,김경수,김명조,심재욱,김병섭,이민웅,이윤수 ( Hei Sun Choi,Kyong Soo Kim,Myong Jo Kim,Jae Ouk Shim,Byong Sup Kim,Min Woong Lee,Youn Su Lee ) 한국균학회 1997 韓國菌學會誌 Vol.25 No.3

5 ' - Deoxy - 5 ' - Iodoinosine 존재하에 사람 적혈구에서의 5 - Iodoribose 1 - phosphate 대사

최혜선,요하나 스토클러 ( Hye Seon Choi,Johanna D . Stoeckler ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.3

5`-deoxy-5`-iodoadenosine (5`-IAdo) and 5`-deoxy-5`-iodoinosine (5`-IIno) were highly cytotoxic to several cell lines in vitro. These studies indicated that the pentose 1-phosphate generated from the 5`-iodonucleosides is responsible for the cytotoxicity. The synthesized and purified 5-iodoribose 1-phosphate (5-IR-1-P) inhibited several enzymes which are related to purine, pyrimidine and sugar phosphate metabolism. Intracellular concentrations of 5-IR-1-P were measured in human erythrocytes through which are in transit to a target site. 560 uM of 5`-IIno generated 500 uM 5-IR-1-P in human erythrocytes. Intracellular concentrations of 5-IR-1-P reached plateau levels in less than 1 hr which remained unchanged for 4 hr. Following removal of extracellular 5`-IIno, the intracellular concentrations of 5-IR-1-P decreased from 500 uM to 300 uM. Intracellular concentration of 5-IR-1-P declined progressively during the subsequent incubation, indicatng that rapidly continuing degradation of 5-IR-1-P occurred in the absence of further synthesis of 5-IR-1-P from 5`-IIno.

MTA 유사체의 Purine Metabolism 에의 영향

최혜선,J . D . Stoecker ( Hye Seon Choi,J . D . Stoeckler ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1

5`-Deoxy-5`-methylthioadenosine (MTA) which is related to polyamine synthesis, is rapidly converted to 5-methylthioribose 1-phosphate (MTR-1-P) and adenine by the reaction of MTA phosphorylase (MTAPase) (Savarese et al., 1981). MTA analogs in which the ribose moiety was replaced have demonstrated to be cytotoxic (Savarese et al., 1984; Ferro et al., 1982). These results indicated that the common product, 5-modified sugar phosphate, exerted cytotoxic effect against several tumor cells. L5178Y mouse lymphoblastic leukemia cells were treated with 5`-modified adenosines, MTA, 5`-deoxy-5`-isobutylthioadenosine (SIBA), 5`-deoxy-5`-iodoadenosine (5`-IAdo) and 5`-deoxyadenosine (5`-dAdo). The phosphorolysis of 100 μM of 5`-modified nucleosides measured in intact L5178Y cells agreed well with the relative V_m of MTAPase from Sarcoma 180 cells determined by Savarese et al. (1983). 5`-IAdo showed extra inhibition of 5-phosphoribosyl 1-pyrophosphate (PRPP) synthesis at high concentration, but the inhibition by 5`-dAdo, MTA and SIBA could be due to adenine released from 5`-substituted adenosines. 5`-Modified adenosines caused greater inhibition of purine synthesis de novo than the same concentration of adenine as were generated from the 5`-substituted adenosines. No inhibition of purine salvage pathway was observed. 5`-IAdo was most cytotoxic and inhibited purine metabolism to the greatest extent in L5178Y cells. In the HL-60 human promyelocytic leukemia cells and HL-60, APRT-deficient mutant cells, the effects of 5`-IAdo on purine and pyrimidine metabolism were measured. 5`-IAdo had no significant effects on PRPP accumulation, purine synthesis de novo and purine salvage pathway.

Kinetics of Intracellular Adenosine Deaminase to Substrate Analogs and Inhibitors in Aspergillus oryzae

최혜선,Choi, Hye-Seon The Microbiological Society of Korea 1994 미생물학회지 Vol.32 No.1

Adenosine deaminase (ADA)의 여러 기질과 억제물질의 반응 속도론적 상수가 Aspergillusoryzae의 세포내 효소인 ADA의 활성자리에 어떻게 부착하고 어떤 요인을 필요로 하는지를 알기위해 측적되어졌다. kcat/$K_m$값에 의하면 조사된 기질로 작용하는 화합물 중에 3'-deoxyadenosine이 가장 좋은 기질로 작용하는 것으로 밝혀졌다. 몇 개의 유사체가 억제물질로 조사되었는데 purine riboside가 $3.7{\times}10^{-5}$M의 값 $K_i$값을 가지고 가장 강한 억베물질로 나타났다. Adenine은 기질로도 억제물질로도 작용을 못하므로 adenine의 N-9 위치의 ribose가 효소에 부착하는데 중요하다는 것을 시사하고 있다. 또 ADA는 6-chloropurine riboside(6-CPR)의 dechlorination을 촉매화하여 inosine과 Cl이온을 생성한다. 6-CPR의 ADA에 대한 기질 특이성은 정상 기질인 adenine의 0.86%로 측정되었다. ADA의 경쟁적 억제물질인 purine riboside는 비슷한 $K_i$값을 가지고 dechlorination도 억제하므로 deamination과 dechlorination 반응은 효소의 부착자리를 공유하고 있다고 생각되어진다. SH기에 작용하는 화합물중 수은제인 p-chloromercuribenzoate(PCMB), mersalyl acid, $HgCl_2$는 효소의 deamination 반응을 억제했다. Mersalyl acid에 의해 활성이 억제된 ADA는 thiol reagent인 dithiothreitol이나 2-mercaptoethanol에 의해 활성이 회복되지만 PCMB에 의해 억제된 효소는 회복되지 않았다. 각 수은제들이 억제물질로 작용할 때 $K_i$값과 억제양상이 측정되었는데 모두 경쟁적 방해를 보였다. $K_i$값은 10mM 인산완충용액에서 측정한 것이 100 mM 인산완충용액에서 측정한 것보다 훨씬 낮아 인산기가 기질이 아니어도 효소의 부착에 큰 영향을 미치는 것을 보여주고 있다. Kinetic parameters of various substrates and inhibitors were measured to elucidate the binding requirements of the active site of intracellular adenosine deaminase (ADA) in Aspergillus oryzae. 3'-Deoxyadenosine was the best substrate according to the value of relative kcat/$K_m$. Purine riboside was found to be the strongest inhibitor with the $K_i$ value of $3.7{\times}10^{-5}$M. Adenine acted neither as a substrate nor as an inhibitor, suggesting the presence of ribose at N-9 of adenosine was crucial to binding. ADA also catalyzed the dechlorination of 6-chloropurine riboside, generating inosine and chloride ions. Substrate specificity of 6-chloropurine riboside was 0.86% of adenosine. Purine riboside, a competitive inhibitor of ADA, inhibit the dechlorination with similar $K_i$ value, suggesting that the same binding site was involved in deamination and dechlorination. Among the sulfhydryl group reagents, mercurials, pchloromercuribenzoate (PCMB), mersalyl acid and $HgCl_2$ inactivated the enzyme. Mersalyl acid-inactivated ADA was reactivated by thiol reagents, but PCMB-inactivated enzyme was not. When ADA was treated with the mercurial reagents, the inhibition constants and inhibition patterns were determined. Each inhibition was competitive with substrate. The $K_i$ values of these mercurial reagents were lower in 10 mM phosphate buffer than in 100 mM phosphate buffer, showing phosphate dependency.

논문편 : 시판 임산부용 거들의 착용감 및 착용효과에 관한 연구

최혜선,이경화 ( Hei Sun Choi,Kyong Hwa Yi ) 대한가정학회 1991 Family and Environment Research Vol.29 No.3

학령기 아동의 스트레스와 대처 및 자살 생각: 대구.경북 지역을 중심으로

최혜선,이미경,Choi, Hye-Seon,Lee, Mi-Kyoung 한국학교보건학회 2012 韓國學校保健學會誌 Vol.25 No.1

Purpose: The purpose of this study was to investigate the stress, coping and suicidal ideation among school-aged children and provide evidence for developing a stress management program for them. Methods: Data were collected and analyzed from 308 fifth graders from 6 elementary schools located in Daegu/Gyeongbuk. Results: First, for stress by general characteristics, there were significant differences in economic status, school record, self-rated health and life satisfaction. Also, subcategory stress by gender, there was a significant difference in appearance stress. Second, passive/avoidant coping had a significant difference in gender. Social support seeking coping and passive/avoidant coping were significant differences in economic status. Active coping had a significant difference in school record. Passive/avoidant coping had a significant difference in self-rated health. Aggressive coping, active coping, social support seeking coping and passive/avoidant coping had significant differences in self-rated life satisfaction. Third, stress and coping had significant differences by suicidal ideation. Fourth, stress showed positive correlations with aggressive coping and passive/avoidant coping while revealing negative correlation with active coping. Conclusion: These results from the study suggest difference by gender and need more active and positive coping strategy for suicide prevention.

An Adaptive Hot-spot Operating Scheme in Vertically Overlaid OFDMA Wireless Systems

최혜선,정희정,김낙명,Choi Hye-Sun,Chung Hee-Jeong,Kim Nak-Myeong The Korean Institute of Communications and Informa 2005 韓國通信學會論文誌 Vol.30 No.7a

OFDMA 하향링크 시스템에서 단말의 불균등한 위치 분포와 다양한 QoS 요구사항의 변화에 따라 시스템에 끼치는 부정적인 영향을 줄이기 위한 적응적 hot-spot 운용 기법에 대해 제안하였다. 매크로셀의 기지국은 셀 내 피고셀의 운용을 조절하며, 예측된 user outage probability와 AHOS 이득 파라미터 값에 의해 피코셀을 켜고 끄게된다. 제안된 AHOS 기법은 다양한 시스템 환경에서 QoS outage probability를 유지하면서 시스템의 throughput을 최대화 시키는 이득을 보였다. We develop an adaptive hot-spot operating scheme(AHOS) to mitigate the negative effects from the nonuniform distribution of user location and the variation in the mixture of QoS requirements in OFDMA downlink systems. The base station in a macrocell can control the operation of picocells within the cell, and turns on or off according to the changes in the estimated user outage probability and the AHOS gain parameter. With the computer simulation, the AHOS has been proved to maximize the system throughput while maintaining the QoS outage probability very low under various system scenarios.

중년여성 기성복의 치수체계에 관한 연구

최혜선,이경미 ( Hei Sun Choi,Kyung Mi Lee ) 대한가정학회 1995 Family and Environment Research Vol.33 No.1

Metabolism of 5-Iodoribose 1-phosphate (5-IR-1-P) in Human Erythrocytes in the Presence of 5'-Deoxy-5'-iodoinosine

최혜선,요하나 스토클러,Choi, Hye-Seon,Johanna, D. Stoeckler Korean Society for Biochemistry and Molecular Biol 1990 한국생화학회지 Vol.23 No.3

5-IR-1-P는 여러 종류의 세포에 강력한 독성을 보이는 5'-iodonucleoside에서 생성이 되어지는데 이 때 생성된 5-IR-1-P는 purine, pyrimidine 및 sugar phosphate의 대사에 관계하는 여러 효소의 활성을 억제시켰다. 목표가 되는 암세포로 가는 통로가 되어지는 적혈구에서 purine nucleoside phosphorylase(PNP)의 활성이 높으므로 5'-deoxy-5'-iodoinosine(5'-IIno) 존재하에 5-IR-1-P의 농도가 측정되었다. $560{\mu}M$의 5'-IIno는 $'500{\mu}M$의 5-IR-1-P를 생성한다. 5-IR-1-P의 세포내 농도는 1시간 안에 최대 수준에 도달하여 4시간 동안 변하지 않는다. 바깥에서 공급되는 5'-IIno를 제거하면 5-IR-1-P의 세포내 농도는 $'500{\mu}M$에서 $'300{\mu}M$으로 감소한다. 이미 세포속으로 들어간 5'-IIno만이 존재할 때 5-IR-1-P의 세포내 농도는 점차적으로 감소한다. 이 사실은 5'-IIno이 계속 공급되지 않으면 5-IR-1-P가 빨리 다른 물질로 대사되거나 파괴되는 것을 의미한다. 5'-deoxy-5'-iodoadenosine (5'-IAdo) and 5'-deoxy-5'-iodoinosine (5'-IIno) were highly cytotoxic to several cell lines in vitro. These studies indicated that the pentose l-phosphate generated from the 5'-iodonucleosides is responsible for the cytotoxicity. The synthesized and purified 5-iodoribose 1-phosphate (5-IR-1-P) inhibited several enzymes which are related to purine, pyrimidine and sugar phosphate metabolism. Intracellular concentrations of 5-IR-1-P were measured in human erythrocytes through which are in transit to a target site. 560 uM of 5'-IIno generated 500 uM 5-IR-1-P in human erythrocytes. Intracellular concentrations of 5-IR-1-P reached plateau levels in less than 1 hr which remained unchanged for 4 hr. Following removal of extracellular 5'-IIno, the intracellular concentrations of 5-IR-1-P decreased from 500 uM to 300 uM. Intracellular concentration of 5-IR-1-P declined progressively during the subsequent incubation, indicating that rapidly continuing degradation of 5-IR-1-P occurred in the absence of further synthesis of 5-IR-1-P from 5'-IIno.

온열적 쾌적과 의복의 보온력

최혜선,Choi Hei-Sun 대한설비공학회 1987 설비저널 Vol.16 No.3