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최혜선,요하나 스토클러,Choi, Hye-Seon,Johanna, D. Stoeckler Korean Society for Biochemistry and Molecular Biol 1990 한국생화학회지 Vol.23 No.3
5-IR-1-P는 여러 종류의 세포에 강력한 독성을 보이는 5'-iodonucleoside에서 생성이 되어지는데 이 때 생성된 5-IR-1-P는 purine, pyrimidine 및 sugar phosphate의 대사에 관계하는 여러 효소의 활성을 억제시켰다. 목표가 되는 암세포로 가는 통로가 되어지는 적혈구에서 purine nucleoside phosphorylase(PNP)의 활성이 높으므로 5'-deoxy-5'-iodoinosine(5'-IIno) 존재하에 5-IR-1-P의 농도가 측정되었다. $560{\mu}M$의 5'-IIno는 $'500{\mu}M$의 5-IR-1-P를 생성한다. 5-IR-1-P의 세포내 농도는 1시간 안에 최대 수준에 도달하여 4시간 동안 변하지 않는다. 바깥에서 공급되는 5'-IIno를 제거하면 5-IR-1-P의 세포내 농도는 $'500{\mu}M$에서 $'300{\mu}M$으로 감소한다. 이미 세포속으로 들어간 5'-IIno만이 존재할 때 5-IR-1-P의 세포내 농도는 점차적으로 감소한다. 이 사실은 5'-IIno이 계속 공급되지 않으면 5-IR-1-P가 빨리 다른 물질로 대사되거나 파괴되는 것을 의미한다. 5'-deoxy-5'-iodoadenosine (5'-IAdo) and 5'-deoxy-5'-iodoinosine (5'-IIno) were highly cytotoxic to several cell lines in vitro. These studies indicated that the pentose l-phosphate generated from the 5'-iodonucleosides is responsible for the cytotoxicity. The synthesized and purified 5-iodoribose 1-phosphate (5-IR-1-P) inhibited several enzymes which are related to purine, pyrimidine and sugar phosphate metabolism. Intracellular concentrations of 5-IR-1-P were measured in human erythrocytes through which are in transit to a target site. 560 uM of 5'-IIno generated 500 uM 5-IR-1-P in human erythrocytes. Intracellular concentrations of 5-IR-1-P reached plateau levels in less than 1 hr which remained unchanged for 4 hr. Following removal of extracellular 5'-IIno, the intracellular concentrations of 5-IR-1-P decreased from 500 uM to 300 uM. Intracellular concentration of 5-IR-1-P declined progressively during the subsequent incubation, indicating that rapidly continuing degradation of 5-IR-1-P occurred in the absence of further synthesis of 5-IR-1-P from 5'-IIno.
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5 ' - Deoxy - 5 ' - Iodoinosine 존재하에 사람 적혈구에서의 5 - Iodoribose 1 - phosphate 대사
최혜선,요하나 스토클러 ( Hye Seon Choi,Johanna D . Stoeckler ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.3
5`-deoxy-5`-iodoadenosine (5`-IAdo) and 5`-deoxy-5`-iodoinosine (5`-IIno) were highly cytotoxic to several cell lines in vitro. These studies indicated that the pentose 1-phosphate generated from the 5`-iodonucleosides is responsible for the cytotoxicity. The synthesized and purified 5-iodoribose 1-phosphate (5-IR-1-P) inhibited several enzymes which are related to purine, pyrimidine and sugar phosphate metabolism. Intracellular concentrations of 5-IR-1-P were measured in human erythrocytes through which are in transit to a target site. 560 uM of 5`-IIno generated 500 uM 5-IR-1-P in human erythrocytes. Intracellular concentrations of 5-IR-1-P reached plateau levels in less than 1 hr which remained unchanged for 4 hr. Following removal of extracellular 5`-IIno, the intracellular concentrations of 5-IR-1-P decreased from 500 uM to 300 uM. Intracellular concentration of 5-IR-1-P declined progressively during the subsequent incubation, indicatng that rapidly continuing degradation of 5-IR-1-P occurred in the absence of further synthesis of 5-IR-1-P from 5`-IIno.
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