비선형 투자비 함수를 이용한 장기 전원개발계획 수립에 관한 연구

김준현(Joon-Hyun Kim),최인홍(In-Hong Choi) 대한전기학회 1988 대한전기학회 학술대회 논문집 Vol.1988 No.11

류마티스양 관절염 환자 활막 T 세포의 T 세포수용체 β쇄 분석

권대호,이수곤,김세종,최인홍,Kwon, Dae-ho,Lee, Soo-Kon,Kim, Se-Jong,Choi, In-Hong 대한면역학회 2001 Immune Network Vol.1 No.1

Background: Rheumatoid arthritis is an autoimmune disease characterized by a chronic inflammatory process, primarily involving the synovial membrane of peripheral j oints, where T cell activation is found. To address the superantigen stimulation in rheumatoid arthritis, T cell clonality and the expression of activation markers were analyzed. Methods: To detect TCRB V usage, inverse PCR and sequencing were done. Monoclonal antibodies were used for flow cytometric analysis of TCRBV8 or TCRBV5. As results, a restricted usage of TCRBV3 gene was detected in synovial lymphocytes from one rheumatoid arthritis patient. However, preferential usage for TCRB V8, which may be one indicator for stimulation by staphylococcal superantigen, was not obvious although general activation of T cells was found as high DR+ percentage in synovial T cells. These data show specific antigen rather than superantigen might involve the pathogenesis of rheumatoid arthritis.

결핵균 단백항원 자극에 의한 대식세포의 TNF-${\alpha}$ 및 IL-6 생성과 ERK 활성화

안혜정,조상래,백태현,이정림,최인홍,Ahn, Hae-Jeong,Cho, Sang-Nae,Paik, Tae-Hyun,Lee, Jung-Lim,Choi, In-Hong 대한면역학회 2007 Immune Network Vol.7 No.1

Background: Mycobacterial antigens released as PIM, LM, LAM, lipoproteins and other cellular factors may contribute to macrophage and dendritic cell activation through pattern recognition receptors such as TLRs. In this study, we assessed cytokine production and ERK activation with stimulation of several major mycobacterial antigens. Methods: Purified mycobacterial antigens (10, 22, 30, 38kDa) and recombinant antigens (6, 16, 19, 38kDa, Ag85A antigen) were studied. The production of cytokines (TNF-${\alpha}$, IL-12, IL-6) was measured by ELISA. The ERK activation was detected by western blotting. The expression of TLR2 or TLR4 was measured by flow cytometry. Results: Among purified antigens only 30kDa antigen induced production of IL-6 or TNF-${\alpha}$ in THP-1 macrophage cells. When THP-1 macrophage cells were treated with 30kDa antigen, phosphorylation of ERK was detected. ERK activation also occurred in TLR2 transfectant HEK293 cells with 30kDa antigen stimulation. Conclusion: 30kDa antigen is one of the major mycobacterial antigens inducing cytokine production and MAP kinases phosphorylation in macrophages.

한국인 IgA 신병증 환아에서 MHC Class II유전자형과 예후와의 관계 분석

김병길,육진원,김지홍,장윤수,신전수,최인홍,Kim Pyung Kil,Yook Jinwon,Kim Ji Hong,Jang Yoon Soo,Shin Jeon-Soo,Choi In-Hong 대한소아신장학회 2000 Childhood kidney diseases Vol.4 No.1

Purpose: Our study was designed to investigate the association of MHC Class II (DR, DQ) allele with IgA nephropathy and its significance as a prognostic factor for progression to ESRD Material and Methods: 69 children with IgA nephropathy with normal renal function(serum creatinine $\leq$ 1.5mg/dL) was classified as group A and 70 patients who received renal transplantation due to IgA nephropathy were selected as group B. The HLA-DQB1 and HLA-DRB1 alleles were studied by polymerase chain reaction using sequence specific primers. We have compared the difference in alleles between these two groups and with normal control and also examined any possible effect of the MHC class II genes on the histopathological severity and prognosis of IgAN. Results: Mean age was $8.8{\pm}2.9$ years in group A and $35.0{\pm}15.5$ years in group B. Male to female ratio was 2.8:1 in group A and 2.5:1 in group B. There was a significantly higher frequency of HLA-$DQB1^*03\;and\;DQB1^*05$ in Group B. The frequency of HLA-$DQB1^*0302\;and\;^*05031$ allele had increasing tendency in Group B(P<0.05). HLA-$DRB1^*03\;and\;^*05$ were more common in Group B(P<0.05). HLA-$DRB1^*04$ allele was the most common DR alleles in both group, but there was no statistical significance. There were no significant correlation with MHC class 13 genes on the hjstopathological severity in Group A. Conclusion: In conclusion, $HLA-DQB1^*0302\;and\;HLA-DQB1^*05031 $ allele seemed to be more common in transplanted patients compared to group with normal renal function suggesting that this allele is associated with poor prognosis in IgAN. However larger studies and follow up are required to confirm this due to uncharacterized heterogeneity in etiopathogenesis of IgA nephropathy and possibly one or more than one gene may exert influence in determining susceptibility to the diseases. 목 적 : IgA 신병증은 혈뇨와 단백뇨를 보이는 사구체 질환으로 세계적으로 가장 빈도가 높은 질환중의 하나 이며 우리 나라를 포함한 아시아 지역에서도 높은 발생율을 보이고 있으나 아직 정확한 발생기전이나 원인은 불분명한 상태이다. IgA 신병증은 발병 10년 후 $20-30\%$가 말기 신부전증으로 진행됨이 보고되고 있으나 신부전으로 진행속도는 환자에 따라 매우 다양하기 때문에 특이적인 조직적합항원을 찾아냄으로서 치료 및 예후 결정에 도움을 주기 위하여 본 연구를 시행하였다. 대상 및 방법 : 세브란스 병원에 입원하여 IgA 신병증으로 진단 받은 환자를 대상으로 정상 신기능을 유지하고 있었던 소아환아 69명(Group A)과 이미 신부전으로 진행하여 신이식을 시행 받은 70명(Group B)을 포함한 신기능 저하군으로 분류하여 HLA대립유전자의 결정은 SSOP (sequence specific oligonucleotide probe)법으로 분석하여 HLA-class II(DR, DQ)대립유전자의 발현빈도와 IgA 신병증의 예후와의 관계를 분석하였다. 결 과 : 대상 환자의 진단 당시의 평균나이는 A군은 $8.8{\pm}2.9$세, B군은 $35.0{\pm}15.5$세였고, 남녀비는 각각 2.8:1과 2.5:1이었다. HLA-DQB1 유전자중 139명중 $47.5\%$로 $03^{**}$의 발현빈도가 가장 증가되어 있었고 $06^{**}(32.4\%),\;04^{**}(28.7\%),\;05^{**}(20.1\%),\;02^{**}(8.6\%)$순이 었다. DQ7의 HLA-DQB1*0301 이 전체 환자의 20.1$\%$(28/139)로 가장 흔한 대립유전자였으나 두군간에 유의한 차이는 없었다. 대립유전자중 HㄴA-$DQBI^*03^{**}과\;DQB1^*05^{**}$가 이미 말기신부전으로 진행된 Group B에서 유의하게 증가되어 있었으며(P<0.05), $03^{**}$ 아형중 $DQB1^{*}0302와\;05^{**}중\;DQB1^{*}05031$이 신기능이 정상인 A군에 비해 유의하게 증가되어 있었다(P<0.05). DR 유전자형의 low-frequency type상 HLA-$DRB1^*03과\;HLA-DRB1^*15$가 Group B에서 유의 있게 증가되어 있었으나, high-frequency type상에서는 양군 사이에 유의한 차이가 없었다. A군의 경우 신조직 검사의 소견에 따라 4등급으로 분류하여 대립유전자의 발현빈도를 분석하였으나 의미 있는 관계가 없었다. 곁 론 : IgA신병증의 예후를 예측하기 위한 여러 조사에서 발병 초기의 심한 단백뇨, 고혈압, 조직 병리학상의 심한 변화 등이 있을 경우 예후가 나쁘다고 하였으며 이런 위험 인자외에 유전적 요인에 대해 알아보고자 하였다. 본 연구에서 신부전으로 진행하여 신대체요법을 받은 IgA신병증환자군에서 HLA-$DQB1^*0302와\;^*05031$ 대립유전자의 발현빈도가 유의하게 증가되어 있어 이 결과를 바탕으로 치료후의 경과 및 예후를 진단초기에 예측하여 환자의 관리에 적용할수 있는 중요한 자료로 이용될 수 있을 것으로 사료된다.

개의 정서발달을 위한 웨어러블 디바이스, GATWALK

이예나(Yena Lee),이지은(Jieun Lee),이장희(Janghee Lee),조휴담(Hudam Cho),최인홍(Inhong Choi),김진우(Jinwoo Kim) 한국HCI학회 2014 한국HCI학회 학술대회 Vol.2014 No.12

국내 애견 관련 산업은 2012 년 기준 이미 2 조원이라는 큰 시장을 형성하였고, 국내의 애완동물을 키우는 인구수는 1,000 만 명에 육박한다. 앞으로도 애견 관련 산업은 지속적으로 성장할 것으로 예상된다. 그런데 많은 애견인들은 바쁜 라이프 스타일 때문에 애견을 충분히 돌보지 못하고 있다. 애견의 분리불안 증세나 우울증 등의 문제를 해결하기 위하여 애견 유치원, 호텔, 애견용 TV 채널 등 다양한 해결책이 나왔으나 아직 획기적인 해결책은 나오지 않았다. 우리는 이 문제에 주목하여 ‘애견의 정서발달을 쉽게 관리할 수 있는 웨어러블 디바이스’인 GATWALK (개 + Network + Walk 의 합성어)를 기획하였다. The market of pet industry is formed as big as about 16 billion dollars, and the population of pet holder reaches 10 million in Korea. And it’s expected to be growing. But many of pet holders are not able to take good care of their pets because of hectic lifestyle. In this context, we designed GATWALK, a wearable device that helps pet to have better emotional status and relationship with the pet owner.

ROS generation and cell toxicity of BEAS-2B-cell-treated particulate matter in ambient air

Soo-Hwaun Kim(김수환),Ji-Yeon Yang(양지연),In-Hong Choi(최인홍),Ji-Young Jang(장지영),Young-Wook Lim(임영욱),Dong-chun Shin(신동천) 한국환경독성학회 2010 한국독성학회 심포지움 및 학술발표회 Vol.2010 No.11

결핵균 단백항원 자극에 의한 대식세포의 TNF-α 및 IL-6 생성과 ERK 활성화

안혜정(Ahn, Hae-Jeong),조상래(Cho, Sang-Nae),백태현(Paik, Tae-Hyun),이정림(Lee, Jung-Lim),최인홍(Choi, In-Hong) 대한면역학회 2007 Immune Network Vol.7 No.1

Mycobacterial antigens released as PIM, LM, LAM, lipoproteins and other cellular factors may contribute to macrophage and dendritic cell activation through pattern recognition receptors such as TLRs. In this study, we assessed cytokine production and ERK activation with stimulation of several major mycobacterial antigens. Methods: Purified mycobacterial antigens (10, 22, 30, 38kDa) and recombinant antigens (6, 16, 19, 38kDa, Ag85A antigen) were studied. The production of cytokines (TNF-α, IL-12, IL-6) was measured by ELISA. The ERK activation was detected by western blotting. The expression of TLR2 or TLR4 was measured by flow cytometry. Results: Among purified antigens only 30kDa antigen induced production of IL-6 or TNF-α in THP-1 macrophage cells. When THP-1 macrophage cells were treated with 30kDa antigen, phosphorylation of ERK was detected. ERK activation also occurred in TLR2 transfectant HEK293 cells with 30kDa antigen stimulation. Conclusion: 30kDa antigen is one of the major mycobacterial antigens inducing cytokine production and MAP kinases phosphorylation in macrophages.

결핵환자의 자연살해세포 매개 세포파괴능과 항체의존성 세포파괴능에 관한 연구(1)

김성규 ( Sung Kyu Kim ),오광제 ( Kwang Je Oh ),안철민 ( Chui Min Ahn ),윤여운 ( Yoe Wun Yoon ),손희영 ( Hee Young Sohn ),김기호 ( Ki Ho Kim ),최인홍 ( In Hong Choi ),윤정구 ( Jung Koo Yoon ) 대한결핵 및 호흡기학회 1986 Tuberculosis and Respiratory Diseases Vol.33 No.4

Behcet 증후군의 분류아형과 현증상에 따른 백혈구 이동억제검사 성적

이은소(Eun So Lee),양동순(Dong Soon Yang),이승헌(Seung Hun Lee),방동식(Dong Sik Bang),이성낙(Sung Nack Lee),최인홍(In Hong Choi),이봉기(Bong Ki Lee),윤정구(Jung Koo Youn) 대한피부과학회 1988 대한피부과학회지 Vol.26 No.6

This study was undertaken to investigate the immunological mechanism of Behqet s syndrome, considered to be important in the pathogenesis of the disease. Seventy- three patients with complete, incomplete and suspected types of Behget's syndrotne were tested for leukocyte migration ingibition factor(LIF), one of the lymphokines. The results were as follows : 1. There was no difference between the average LIF activity of all the patients and that of eontrol. 2. LIF activity of complete type, according to Shirnizus classification, was significaritly lower than the control value. 3. LIF activity of ocular type, according to Lehners classification, was signficantly lower than the control value. 4. LIF activity for patients with 4 clinical symptoms was well below the value for patients with less symptomes 5. For patients with single clinical symptom, LIF activity of complete type was well below the values of incomplete and suspected types. 6. In suspected and mucocutaneous types, LIF activity was low when the patients showed two clinical symptoms than one. Thus, LIF activity was low for patients with complete, ocular and neurological types and with multiple symptorns.

B 형 바이러스성 만성 활동성 간염 환자에서 스테로이드 이탈요법 및 알파 인터페론 투여에 의한 면역상태의 변화

이관식(Kwan Sik Lee),한광협(Kwang Hyub Han),정준표(Jun Pyo Chung),전재윤(Chae Yoon Chon),이상인(Sang In Lee),문영명(Young Myung Moon),강진경(Jin Kyung Kang),박인서(In Suh Park),최흥재(Heung Jai Choi),신전수(Jeon Soo Shin),최인홍(In H 대한내과학회 1995 대한내과학회지 Vol.49 No.1

N/A Objectives: It has been suggested that cellular immune responses to the hepatitis B virus are of importance in the production of liver cell damage in chronic active hepatitis type B. Short-term corticosteroid withdrawal in patients with chronic active hepatitis type B is frequently associated with enhanced cellular immune responses to hepatitis B virus. Several clinical studies had suggested that short-term corticosteroid withdrawal followed by interferon treatment might enhance its antiviral efficacy. The present study was designed to investigate the change of immune parameters in patients with chronic active heptitis type B treatment with short-term corticosteroid withdrawal followed by interferon alpha(IFN-a). Methods: The subjects were 11 patients with chronic active hepatitis type B who were given prednisolone in decreasing daily doses of 60mg, 40mg and 20mg, each for 2 weeks, followed by a 4-week rest and l6 weeks of recombinant alpha 2a interferon (INF-α) was administered intramuscularly. Serum ALT level, serum HBV DNA level and serum sIL-2R level were assayed and CD4+ T cell/ CD8+T cell ratio, IL-2R+T cell/Total T cell(%), TLiSAI+T cell/Total T cell(%) and suppressor T cell activity were measured. Results: 1) The serum ALT level was increased significantly at 2 weeks after the end of prednisolone administration(p<0.005) and decreased significantly after the start of INF-α administration(p<0.005). 2) The serum HBV DNA level was increased significantly during the prednisolone administration(p<0.01) and decreased siginificantly after the end of prednisolone administration and resting period(p<0.05). 3) The serum sIL-2R level was increased significantly at 2 weeks after the end of prednisalone administration(p<0.05). After the end of prednisolone administration and the start of INF-α administration, the serum sIL-2R level was decreased significantly( p<0.05). 4) The increase of serum ALT level after the increase of IL-2R+T cell(%) and TLiSA1+ T cell/ Total T cell(%) after the increase of TLiSA1+T cell/ Total T cell(%) was significant. 5) The maximum increase of serum ALT level and the maximum decrease of suppressor T cell activity were observed in 4 of 5 patients(80.0%) at 2 weeks after the end of prednisolone administration. Conclusion: The serum HBV DNA level was decreased significantly by the immune rebound after the end of steroid administration. Probably increase of IL-2R+T cell/Total T cell(%) and TliSAl+T cell/ Total T cell(%) and decrease of suppresor T cell activity were related to the immune rebound.