GM-CSF를 이용한 척수손상의 치료적 접근

최병현 ( Byung Hyune Choi ),하윤 ( Yoon Ha ),박현선 ( Hyeon Seon Park ),윤승환 ( Seung Hwan Yoon ),박형천 ( Hyung Chun Park ),민병현 ( Byoung Hyun Min ),박소라 ( So Ra Park ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.1

서울시 주거 젠트리피케이션 지역 거주민의 주거선택 특성 실증분석

최병현(Choi, Byoung-Hyun),유민태(You, Min-Tae),박태원(Park, Tae-Won) 도시정책학회 2020 도시부동산연구 Vol.11 No.1

In this study, to understand the migration factors of ‘Gentrifier’(the main agents of Gentrification), we conducted a survey of the resident in Namsan Trapalace and Yongsan Parkzai and analyzed their residence selecting factors. And also in this study, sample factors had been extracted through domestic and foreign advanced researches and questionnaires by residents and experts. And actual analysis was done through targeted residents questionnaires. As the results of the analysis, gentrifier’s residence selecting factors are life convenience in the community, financial values of house, locational benefit of downtown, convenience of commercial and cultural facilities, and distance between residence and workplace. Statistically, gentrifiers are well-educated and high-income professional workers at a high rate, in the same as shown in the foreign gentrifications. And also they show the characteristics of Korean gentrification that at a high rate, the household consists of more than two generations and the householder is in their 40’s to 60’s. Thus we can see the gentrifications in Seoul, and to use them as the policy means of inner city, it is suggested that the study of community character and diverse causes should be consistently conducted.

T2a, T2b 위암의 임상양상 분석

최병현(Byoung Hyun Choi),김지훈(Ji Hoon Kim),곽진호(Jin Ho Kwak),장혁재(Hyuck Jae Jang),한명식(Myung Sik Han) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.4

Purpose: T2 gastric cancer is tumor invading proper muscle or subserosal layer. The 2002 American Joint Committee on Cancer subdivided the pT2 gastric adenocarcinoma into a type pT2a (invasion of the proper muscle) and a type pT2b (invasion of the subserosa). The aim of this study was to evaluate the differences between pT2a and pT2b gastric cancers of the clinicopathological features and the prognostic factors. Methods: A series of curative operations were performed on 194 patients with pT2 gastric cancer from January 1999 to February 2006. We reviewed, retrospectively, the clinicopathologic characteristics, the 3-year disease free survival and the 5-year disease specific survival rates. Results: There were significant clinicopathologic differences between pT2a and pT2b gastric cancer in tumor size and the number of metastatic lymph nodes. Both of the 3-year disease free survival time and the 5-year disease specific survival time were much longer for the pT2a gastric carcinoma patients than those for the pT2b gastric carcinoma patients. Conclusion: It is important to consider the differences between pT2a and pT2b gastric cancers for prediction of recurrence or prognosis. Furthermore, new staging of pT2a and pT2b cancer may be necessary.

중간엽 줄기세포의 연골화 분화에 저강도 초음파 자극의 전처리가 미치는 영향

이현정 ( Hyun Jung Lee ),최병현 ( Byung Hyune Choi ),민병현 ( Byoung Hyun Min ),박소라 ( So Ra Park ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.1

To date, the 3-D culture system and TGF-ß? treatment are known as the most critical factors for chondrogenic differentiation of mesenchymal stem cells (MSCs). However, the development of more efficient and versatile methods is still in demand for the clinical application of MSCs in cartilage repair. Recently, low-intensity ultrasound (LIUS) was shown to enhance fracture healing and induce chondrogenesis of MSCs in vitro. We examined the effect of LIUS on the chondrogenic differentiation of rabbit MSCs at the gene expression level. The results showed that the LIUS treatment on a 3-D alginate culture of rMSCs increased 1) the expression of chondrogenic markers such as collagen type II, aggrecan and Sox9 in RT-PCR analysis and 2) the level of collagen type II protein in Western blot analysis. These results suggest that the LIUS treatment facilitate chondrogenic differentiation of MSCs. We speculate that the non-invasive LIUS can be used as a mechanical stimulus to potentiate chondrogenic differentiation of MSCs and hence clinically for the repair of cartilage damage.

도심부 재개발 공동주택의 주거선택요인이 거주만족에 미치는 영향분석

장세준(Jang, Se-Jun),최병현(Choi, Byoung-Hyun),박태원(Park, Tae-Won) 한국지역개발학회 2013 한국지역개발학회 세미나 논문집 Vol.2013 No.1

urban redevelopment is explicit in the city that can be attributed to the spread. 1960s and 1970s, fast industrialization and population growth, housing shortages. For example, Ilsan, Bundang, Pyeongchon, the middle east, and leads to a new town development is such Pangyo, suburbanization phenomenon was caused. Therefore, this study ‘Morning of Kyounghee Palace’, including the old Town is another destination Nam Mountain ‘Trapalace’ to target residents of the Housing Choice survey conducted to identify factors that, based on this Multiple Regression Analysis, the correlation between residential satisfaction and analyzed. it appeared that the residential satisfaction that the residents of urban multipurpose buildings in seoul feel were considerably high in general and it seems to need to increase the value of realestate by rasing quality of interior design of buildings, improving indoor safety, comfort and maximizing the urban attraction such as traffic convenience of running in all directions, advantage from job-housing proximity, affluent urban convenience facilities, affluent parks, resting space like Namsan, old palace.

Preplate법에 의해 분리된 근육유래 줄기세포의 지방세포 분화능력

이미애 ( Mi Ae Lee ),최병현 ( Byung Hyung Choi ),민병현 ( Byoung Hyun Min ),박소라 ( So Ra Park ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.2

Muscle satellite cells are traditionally known as a myogenic precursor but also reported to have a multidifferentiation potential into other lineages such as osteoblast and adipocyte. Recently, Qu et al. reported on the isolation and multi-potential differentiation of very limited population of muscle-derived stem cells(MDSCs) in the satellite cells via the preplate method. Here we intended to evaluate the preplate method by examining the proliferation capacity and adipogenic differentiation of MDSCs in comparison with those of the unisolated satellite cells The satellite cells were isolated from the hind limb of newborn mouse and the MDSCs(pp7) and a series of intermediate preplate cell populations (pp1~pp6) were separated by the preplate method. The satellite cells were highly proliferative and fibroblast-like in morphology, which were well maintained during the long-term culture. One of the early preplate cells, pp3, also showed a high proliferation capacity and spindle-like morphology with some myotubes. In contrast, pp5(a late preplate cells) and MDSCs(pp7) showed a limited proliferative capacity. They also showed a spindle morphology at the early time, but gradually changed into the senescence-like morphology. When they were induced into the adipogenesis, the satellite cells and pp3 efficiently formed lipid vacuole and differentiated into the adipocyte morphology. In contrast, pp5 and MDSCs(pp7) showed no adipogenic phenotypes. These results were confirmed by the changes in the expression patterns of PPARc(an adipogenic marker), MyoD and myogenin(myogenic markers) in these cell population during the adipogenesis by RT-PCR analysis. It was of note that pp3 showed high level expression of myogenic markers all the time during efficient adipogenic differentiation. In conclusion, the preplate method was inefficient in isolating the stem cell population from skeletal muscles, which is inconsistent from the previously report. In addition, the muscle satellite cells themselves showed efficient adipogenic differentiation and could be used as potent stem cells without further separation process.

GM-CSF가 골수줄기세포의 이동에 미치는 영향

한동하 ( Dong Ha Han ),곽민근 ( Min Geun Kwak ),최병현 ( Byung Hyune Choi ),하윤 ( Yoon Ha ),박현선 ( Hyun Seon Park ),민병현 ( Byoung Hyun Min ),우제홍 ( Ze Hong Woo ),박소라 ( So Ra Park ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.3

The granulocyte macrophage colony-stimulating factor (GM-CSF) is known to play an important role in the hematopoiesis of bone marrow-derived cells and the modulation of their functions as well as the maintenance of homeostasis and overall immune competence. In this study, we investigated the effects of GM-CSF on the activation of bone marrow stem cells in rats. GM-CSF was treated to SD rats via intraperitoneal injection everyday at 10 mg/ml for 5 consecutive days. After 6 days, the bone marrow (BM) and peripheral blood (PB) mononuclear cells (MNCs) were collected to analyze the colony forming units-fibroblasts (CFU-F), colony forming units-granulocyte macrophage (CFU-GM), and burst-forming units-erythroid (BFU-E). In result, the numbers of MNCs and the CFU-GM and BFU-E counts in BM were all decreased, while those of PB increased. These results suggest that GM-CSF induced the proliferation and mobilization of MNCs and hematopoietic stem cells (HSCs) to PB. The CFU-F counts showed no significant increase both in BM and PB suggesting that GM-CSF showed no significant effects on the proliferation of mesenchymal stem cells. In contrast, the increase in the alkaline phosphatase expression of CFU-F colonies in BM indicates that GM-CSF might induce the differentiation of MSCs at least to some extent. In conclusion, GM-CSF not only plays important roles in proliferation and differentiation of stem cells in BM but also promotes migration of HSCs into PB.

아웃렛쇼핑몰의 공간구성요소와 이용만족도의 상관성 분석

박태원(Park, Tae-Won),이덕조(Lee, Deok-Jo),최병현(Choi, Byoung-Hyun) 한국지역개발학회 2013 韓國地域開發學會誌 Vol.25 No.1

This research is a study on the use of the outlet mall and behavioral characteristics. Case study area was selected as Shinsegae Chelsea Outlet in Paju. The main method of analysis, we conducted a factor analysis and correlation analysis. Factors were analyzed in all seven survey results. A total of seven factors were utilized to analyze the correlation between the use of visitor satisfaction. In addition, prior to analyzing the impact of the components of the space given to the satisfaction of visitors outlet mall, we conducted a correlation analysis between each variable. Analysis of the spatial element affecting the satisfaction of visitors outlet mall, it was found that the variable does not have a significant relationship satisfaction and outlet mall cause strange sociological population. If you look at the relative influence of individual variables of satisfaction outlet mall, pointed out the corelation of the components of the outlet mall space, the (+) static outlet mall human satisfaction, vision, tenant, and then support shopping be a significant impact has been confirmed.

아웃렛쇼핑몰의 공간구성요소와 이용만족도의 상관성 분석

박태원(Park Tae Won),이덕조(Lee Deok Jo),최병현(Choi Byoung Hyun) 한국지역개발학회 2013 韓國地域開發學會誌 Vol.25 No.1

This research is a study on the use of the outlet mall and behavioral characteristics. Case study area was selected as Shinsegae Chelsea Outlet in Paju. The main method of analysis, we conducted a factor analysis and correlation analysis. Factors were analyzed in all seven survey results. A total of seven factors were utilized to analyze the correlation between the use of visitor satisfaction. In addition, prior to analyzing the impact of the components of the space given to the satisfaction of visitors outlet mall, we conducted a correlation analysis between each variable. Analysis of the spatial element affecting the satisfaction of visitors outlet mall, it was found that the variable does not have a significant relationship satisfaction and outlet mall cause strange sociological population. If you look at the relative influence of individual variables of satisfaction outlet mall, pointed out the corelation of the components of the outlet mall space, the (+) static outlet mall human satisfaction, vision, tenant, and then support shopping be a significant impact has been confirmed.

급성 식도정맥류 출혈 환자에서 Somatostatin과 Vasopressin의 치료효과 비교 : A Prospective Randomized Trial

최병현,문병수,문영명,한광협,전재윤,이관식,정정일,김동기,백용한,이천균 대한간학회 2000 Clinical and Molecular Hepatology(대한간학회지) Vol.6 No.4

Background/Aims: Although endoscopic band ligation or injection sclerotherapy are the current standard therapies for bleeding esophageal varices, the best method for initial control is unclear. The aim of this prospective study was to compare the efficacy and toxicity of somatostatin and vasopressin in the management of esophageal variceal hemorrhage. Methods: From March, 1997 to September, 1998, 28 consecutive cirrhotic patients admitted to the Yonsei Medical Center because of active variceal bleeding were included in this trial. The patients were randomized to receive either somatostatin (15 patients) or vasopressin (13 patients) for 48 hours. Results: There were no significant differences between the two treatment groups in relation to Child's classification, amount of bleeding before randomization and units of blood transfused during therapy. Initial control of bleeding was achieved in 13 (86.7%) patients receiving somatostatin and in 10 (76.9%) of those treated with vasopressin. However, two patients in the somatostatin group and two in the vasopressin group bled again during treatment. Therefore complete control of bleeding during the 48 hours of therapy was achieved in 11 (73.4%) patients treated with somatostatin and in eight (61.5%) of those receiving vasopressin. Differences were observed in complications associated with each therapy. Vasopressin produced complications in four patients (chest pain in two, hypertension in one and hyponatremia-induced seizure in one) while somatostatin produced minor complication in one patient. Conclusions: This study suggests that somatostatin is efficacious in controlling acute hemorrhage from esophageal varices and has a lower risk of adverse effects than vasopressin.(Korean J hepatol 2000;6;468-473)