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Jaw Opening Reflex 및 RT-PCR을 이용한 봉독의 진통효과
조광호,이재동,박동석,안병철,Cho, Kwang-Ho,Lee, Jae-Dong,Park, Dong-Suk,Ahn, Byoung-Choul 대한약침학회 2000 Journal of pharmacopuncture Vol.3 No.1
The purpose of this study is to prove the analgesic effects of apitoxin and its mechanism via jaw-opening reflex(JOR) and measuring expression of mRNA in Phospholipase and Tryptophan hydroxylase(TPH) using RT-PCR. The experiments were carried out on Sprague-Dawley rats(300-400g) and mastocytoma(P-185 HTR) for JOR and RT-PCR, respectively. Rats anesthetized with thiopental sodium (80mg/kg) were used in the Tooth Pulp stimulation induced JOR. The amplitude of a digastric electromyogram (dEMG) was recorded during the stimulation at an intensity of 1.5 times the threshold for JOR. Apitoxin used in this experiment was diluted with normal saline by 1:1000. Apitoxin was injected intravenously into the test group while normal saline to the control group. However, it was injected directly into the cell of mastocytoma. We referred to base sequence registered in Genbank in designing primers for RT-PCR. The results were as follows; (1)Compared with control group, analgesic effect started to show right after Sprague-Dawely rats were treated with apitoxin($71.50{\pm}8.08$) and lasted for 50 minutes. (2)As a result of the experiment of RT-PCR, we witnessed significant changes in the degree of expression of phospholipase or rate-limiting enzyme of biosynthesis of prostaglandins with $10{\mu}g/ml$ apitoxin.($31.74{\pm}18.98%$, P<0.05) (3)As a result of the experiment of RT-PCR, we witnessed significant changes in the degree of expression of TPH or rate-limiting enzyme in biosynthesis of serotonin with $10{\mu}g/ml$ apitoxin.($131.37{\pm}16.87%$, P<0.05). These results suggest that $10{\mu}g/ml$ apitoxin have the most analgesic effects. This study showed that apitoxin has analgesic effects and held good for 50 minutes. The injection of apitoxin has brought out changes in the degree of expression of phospholipase and TPH. These results strongly suggest that analgesic mechanism by apitoxin is closely related to prostaglandins and serotonin.
조광호,박상옥,박래준,Cho, Kwang-Ho,Park, Sang-Ock,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.1
We found out that there exists threshold dose for the decreased platelet count when we apply change of the platelet count to the defense of the ultrasound experiment. Those diminution is definite effects from the defense of the ultrasound. The number of the platelet count is decreased when we are exposed to ultrasound simultaneously. This should not be occurred. It is easy to use the number of the platelet count in the measurement of the biological dosage and intensity in high dosage relatively due to definite quantative relationship. The biological index to understand synergic effects. which are present when two harmful environmental factors are operating at the same time is functionally assending and antagonistic. There are scattered many factors as physical, Chemical, or physiochemically elements which are harmful to human body in the environment in consideration of ultrasound and the other factors. This experiment is demonstrating that we can use the change of the platelet as an index representing synergic effects of ultrasound.
사료포중심 낙농경영에서 조사료자급을 위한 작부유형에 관한 연구 ( 전남지역 실태를 중심으로 )
조광호(Kwang Ho Cho),전우복(Woo Bock Chun),조성균(Seong Gyun Cho) 한국축산학회 1987 한국축산학회지 Vol.29 No.1
This study was carried out to determine the best condition of roughage supply for the efficient management of farm land for forage production. To investigate the present situation of feed production in forage crop field, 31 farms were surveyed in Chonnam province during summer season of 1985. The results obtained were as follows; 1. Production of forage crops in upland was more profitable than that of other crops such as red pepper, garlic, Irish potato, barley and soybeans. 2. The optimum combination of feed for a milk cow was determined as 2,000㎏ of concentrate and 4,435㎏ of hay when a cow produced 5,000㎏ of milk a year. 3. Minimum land requirement for self-sufficiency of roughage to feed a cow was 0.17㏊ of forage crop field without feeding any rice straw, and 0.14㏊ of forage crop field with feeding 1,000㎏ of rice straw a year. 4. Reasonable cropping pattern of forage with Italian ryegrass, sorghum-sudangrass hybrid, corn, rye, radish and rape were suggested for typical dairy farming to attain efficient use of land and self-supply of feed.