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Rapamycin이 T 림프구의 세포주기 조절에 미치는 영향
이정삼(Jeong Sam Lee),한철(Chul Han),최수진나(Soo Jin Na Choi),정상영(Sang Young Chung) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.73 No.1
Purpose: Rapamycin (RPM) and its analogues are known for their potent immunosuppressant and anti-proliferative properties, which stem from their ability to modulate the signal transduction pathways involved in cell cycle progression from the G1 to S phase. Thus, RPM has been shown to inhibit the proliferation of a number of non-immune cell types, including hepatocytes, vascular smooth cells and fibroblasts. In addition to its effects on proliferation, RPM may also play a role in the regulation of apoptosis under certain circumstances. Methods: The effects of RPM on the activation, proliferation and expression of cytotoxic effector molecules were examined on Molt-4 human T-lymphocyte by determining its effects on apoptosis, cell viability, reactive oxygen species (ROS) production and mitochondrial dysfunction. Cells were cultured in the presence or absence of RPM, and then analyzed by Flow cytometry after staining with PI (propidium iodide). Results: The viability of Molt-4 T cells dose- and time-dependently decreased on the addition of RPM. Conclusion: RPM induced cytotoxicity was characterized by G2/M phase cell cycle arrest. In addition, a pharmacological scavenging study of ROS, including H₂O₂, revealed the cytotoxicity was mainly induced by the generation of ROS, which might modulate the expression of Bak protein and mitochondrial dysfunction.
우관제(Kwan-Je Woo),정상영(Sang-Young Jeong),김용원(Yong-Won Kim) 한국철도학회 2015 한국철도학회 학술발표대회논문집 Vol.2015 No.10
차량이 노후화 됨에 따라 차량 안전성에 관련된 요소들의 특성이 변화하는 경향이 있다. 특히 타고오름탈선에 관련된 1차 스프링, 2차 스프링의 강성은 차량 노후화에 따라 강성이 커져 탈선의 위험성이 증가하게 된다. 또한 도시철도차량 안전기준에 관한 규칙발효 이전에 제작된 전동차는 윤중비 관리가 이루어지지 않아 수 십 %의 윤중비가 측정을 통해 확인되었다. 본 논문에서는 차량의 노후화에 따른 현수장치 특성 변경 및 도시철도차량 안전기준에 관한 규칙 발효 이전에 설계/제작된 차량의 실제 윤중비로 인한 타고오름탈선의 위험성에 대해 연구하였다 As the operation time of the train increases, the characteristics of the parts which have effect on the safety of the trains change. Especially, the stiffness of the primary and secondary springs has a tendency to increase with the aging so the possibility of the wheel climbing derailment increases. And the trains which designed and built before the domestic regulation which specifies the limit values of the wheel load ratio became effective, the measured wheel load ratio are as high as 20%~30%. In this paper the possibility of the wheel climbing derailment was studied with the changes of the characteristics of the suspension and wheel off-load ratio of the degraded trains.
양측 요관 폐쇄 후 흰쥐 신장 Aquaporin 단백 발현의 변화
정순주(Soon Ju Jeong),정상영(Sang Young Chung),남광일(Kwang Il Nam),이승원(Seung Won Lee),박성식(Sung Sik Park),안규윤(Kyu Youn Ahn) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.4
목 적: 요로폐색은 신 기능 상실의 주된 원인 중의 하나이다. Aquaporin은 신장을 비롯한 여러 조직에서 발현되는 수분통로 단백이다. 본 연구는 양측 요관폐쇄 후 AQP-1, 2, 3 및 4 수분통로 단백 발현의 변화와 분포를 알아보고자 하였다. 방 법 : 동물은 실험군과 대조군으로 나누었다. 실험군은 복강을 열고 2-0 봉합사로 양쪽 요관 근위부를 결찰하였고 대조군은 결찰을 제외하고는 동일한 처치를 하였다. 복부를 봉합하고 48 시간 동안 물과 식이를 공급한 후 희생시켰다. 적출된 신장조직에서 AQP-1, 2, 3 및 4에 대한 면역조직화학 실험을 행하였다. 결 과 : 대조군 신장에서 AQP-1의 면역반응성은 피질과 외수질 외대의 근위세관, 외수질 내대와 내수질의 Henle 고리 세각의 첨부세포막과 기저외세포막에서 관찰되었다. 실험군에서의 발현부위는 변화가 없었으나, 면역반응성은 근위세관과 Henle 고리 세각 모두에서 감소하였고 그 정도는 근위세관과 내수질의 파괴가 심한 부위의 Henle 고리 세각에서 현저히 감소하였다. 대조군 AQP-2의 면역반응성은 전 집합관 주세포의 첨부세포막과 세포질에서 강하게 발현되었다. 실험군의 AQP-2의 발현부위는 변화가 없었고, 발현정도는 외수질 내대 집합관을 제외한 나머지 전 집합관에서 감소하였다. 대조군 AQP-3의 면역반응성은 전 집합관의 주세포 기저외세포막에서 강하게 발현되었다. 실험군의 발현 부위는 신 유두상피와 전 집합관으로 대조군과 상이하였다. 발현정도는 전 집합관에서 감소하였다. 대조군 AQP-4의 면역반응성은 외수질 내대와 내수 질집합관 주세포의 기저외세포막에서 중등도로 발현되었다. 실험군의 발현 부위는 전 집합관으로 대조군과 상이하였다. 발현정도는 피질 및 외수질 외대 집합관에서는 중등도였으며, 외수질 내대 및 내수질집합관에서는 집합관에 따라서 다양하였다. 결 론 : 양측 요관폐쇄 후 AQP-1, 2, 3 및 4 수분통로 단백 발현의 변화로 신기능의 변화를 가져올 수 있음을 시사하였다. Background : Obstruction of urinary tract is common cause of renal disfunction. Recent discovery of aquaporin water channels expressed in the kidney and various organs has faciliated our understanding of water transport across the permeable epithelial cell membrane. This study was performed to investigate the effects of bilateral ureteral obstruction on renal expression and cellular distribution of these water channels in rat kidney. Methods : Male Sprague-Dawley rats were divided two groups. The abdominal cavity was opened and 2-0 silk ligatures were proximally placed on both ureters in experimental group. Sham-operated group was treated in the same procedures except ligation. After closure of the abdomen, the animals were maintained for 48 hr while being given food and water ad libitum. Kidney sections of both groups were processed for immunohistochemistry using antibodies to aquaporin-1, 2, 3, and 4. Results : Immunoreactivity for aquaporin-1 of sham-operated kidney was detected in the apical and basolateral plasma membrane of proximal tubules and thin limb of Henle loop. That of bilateral ureteral obstructed kidney was decreased in the both tubules, especially in the proximal tubules and thin limb of Henle loop of inner medulla. Immunoreactivity for aquaporin-2 of sham-operated kidney was the most prominent in apical region and moderate in cytoplasm of the principal cells of entire collecting ducts. That of obstructed kidney was markedly decreased in entire collecting duct, especially inner medulla except inner stripe of outer medulla. The decrease was in parallel between the apical region and cytoplasm. Immunoreactivity for aquaporin-3 of sham-operated kidney was the most prominent in the basolateral plasma membrane of principal cells of entire collecting duct. That of obstructed kidney was decreased in entire collecting duct. Papillary epithelium was stained in obstructed kidney. Immunoreactivity for aquaporin-4 of sham-operated kidney was moderate in the basolateral plasma membrane of principal cells of collecting ducts of inner stripe of outer medulla and inner medulla. In obstructed kidney, immunoreactivity was detected in cortical and outer stripe of outer medullary collecting duct, and decreased in inner stripe of outer medulla and inner medulla. A marked heterogeneity was observed in inner medullary collecting duct. Conclusion : These results indicate that alterations of expression of aquaporin proteins after bilateral ureteral obstruction may lead to change in renal functions, such as urine concentrating ability.