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시마연어, Oncorhynchus masou에서 분리된 아니사키스 속 선충 3기 유충의 분자생물학적 방법을 이용한 동정
전찬혁 ( Chan Hyeok Jeon ),김정호 ( Jeong Ho Kim ),( Eko Setyobudi ) 한국어병학회 2010 한국어병학회지 Vol.23 No.3
Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon (Oncorhynchus masou). Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.
전찬혁 ( Chan Hyeok Jeon ),위성 ( Seong Wi ),김정호 ( Jeong Ho Kim ) 한국어병학회 2014 한국어병학회지 Vol.27 No.1
Hydrolase activities of excretory-secretory products (ESP) and somatic extracts (SE) from Anisakis simplex sensu stricto (s.s.) and Anisakis pegreffii larvae were investigated by using API ZYM kit. In esterase group, acid phosphatase showed high activity from both of A. simplex (s.s.) and A. pegreffii. Esterase (C4) showed activity only from SE and A. simplex (s.s.) showed higher activity than A. pegreffii. Alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase showed higher activity in 3rd stage larvae than in 4th stage larvae of both species. In aminopeptidase group, only leucine arylamidase showed remarkable activity in SE of both anisakid species, and A. simplex (s.s.) SE showed higher activity than A. pegreffii SE. In glycosidase group, N-acetyl-β-glucosaminidase, α-mannosidase, α-fucosidase showed higher activity in A. simplex (s.s.) than A. pegreffii, and 4th larvae showed higher activity than 3rd larvae. These differences in hydrolase activity of anisakid nematodes larvae are thought to be due to different metabolism such as growth, moulting, digestion and feeding.
명태(Theragra chalcogramma) 종묘에서 분리된 Vibrio anguillarum의 생화학적, 분자생물학적 특성
전찬혁 ( Chan Hyeok Jeon ),남우화 ( U Hwa Nam ),김정호 ( Jeong Ho Kim ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.3
The health of Alaska pollock Theragra chalcogramma seedlings was monitored during February and April 2015. After microscopic examination for parasites, 20 samples sets were made by pooling 50 individuals for each sample set. Then, they were homogenized and examined for viral and bacterial pathogens. No parasites or viruses were detected using either microscopy or PCR. Colonies suspected of belonging to the genus Vibrio were isolated from Tryptic Soya Agar and Thiosulfate Citrate Bile Salts Sucrose Agar plate incubations, and identified as Vibrio anguillarum based on biochemical and physiological examinations and PCR amplification of the 16S rDNA, recA, and pyrH genes. Although there was no mortality during the sampling period, 65.0% (13/20) of the pooled samples were PCR-positive for V. anguillarum. To prevent possible outbreaks, the pathogenic potential of V. anguillarum should be investigated in the future.
청자갈치( Bothrocara hollandi)의 근육에 기생하는 점액포자충 Myxobolus aeglefini (Myxozoa: Myxobolidae)
전찬혁 ( Chan Hyeok Jeon ),김정호 ( Jeong Ho Kim ) 한국어병학회 2015 한국어병학회지 Vol.28 No.2
동해안에서 어획된 청자갈치 (Bothrocara hol-landi)의 체측 근육에 유백색의 불투명한 시스트를 형성하고 있는 점액포자충이 발견되었다. 시스트를 마쇄하여 광학현미경으로 관찰해본 결과, 원형에 가까운 점액포자충의 성숙 포자가 관찰되었다. 성숙 포자의 평균 길이는 11.9 (11.0~13.5) μm, 평균폭은 11.6 (10.7~13.6) μm, 평균 두께는 7.8 (6.9~8.8)μm이었다. 극낭의 평균 길이는 4.4 (3.2~5.3) μm이었으며, 평균 폭은 평균 3.3 (2.4~4.2) μm이었다. 감염숙주와 성숙포자의 형태학적 특징, 각 부위의 측정값으로부터 본 연구에서 발견된 점액포자충은 Myxobolus aeglefini Auerbach 1906으로 동정하였다. 또한 18S rDNA sequences를 이용한 계통분석결과 M. albi와 M. groenlandicus와 같은 분지에 속하는 것이 확인되었으며 각각 97.7%와 96.9%의 상동성을 나타내었다. A specimen of porous-head eelpout Bothrocara hollandi (Zoarcidae: Perciformes) caught from the East Sea was found to harbour a myxosporean parasite. Numerous whitish pseudocysts were scattered throughout the body musculature of this individual specimen. Fresh myxosporean spores were found from the squashed pseudocysts under light microscopy. They were subspherical in frontal view with a length of 11.9 (11.0~13.5) μm, width of 11.6 (10.7~13.6) μm, and thickness of 7.8 (6.9~8.8) μm. Two polar capsules were almost equally pyriform with a length of 4.4 (3.2~5.3) μm and width of 3.3 (2.4~4.2) μm. Morphometric and host ecology analysis revealed that this myxosporean parasite could be identified as Myxobolus aeglefini Auerbach 1906. Phylogenetic analysis based on 18S rDNA sequences also revealed that M. aeglefini was clustered with M. albi and M. groenlandicus in the same branch, sharing 97.7% and 96.9% sequence similarities with M. albi and M. groenlandicus, respectively.
2010∼2012년 연안에서 서식하는 해산어에서 아니사키스 유충의 감염현황
김위식 ( Wi Sik Kim ),전찬혁 ( Chan Hyeok Jeon ),김정호 ( Jeong Ho Kim ),김도형 ( Do Hyung Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
A survey was conducted to investigate infection of anisakid nematode larvae in 243 wild marine fishes caught from the southern coastal area of Korea between 2010 and 2012. The samples comprised fishes from 9 orders, 30 families and 50 species. Total infection rate of anisakid nematode larvae was 10.7% (26/243 fish), which comprised from Yeosu, 7.4% (7/95) in 2010 and 22.7% (5/22) in 2011; from Jeju, 8.2% (5/61) in 2011; from Wando, 40.9% (9/22) in 2012. Anisakid nematode larvae were not detected in Tongyoung and Wando samples in 2011. Molecular identification of the 89 worms from 26 fish was conducted by PCR-RFLP and/or sequence analysis of internal transcribed spacer (ITS) region of ribosomal DNA. From the results, 6 kinds of anisakis species were identified: Anisakis pegreffii (infection rate: 53.9%, 48/89 worms), Hysterothylacium aduncum (38.2%, 34/89), H. fabri (3.4%, 3/89), hybird (A. simplex X A. pegreffii) (2.4%, 2/89), A. simplex (1.1%, 1/89) and Raphidascaris lophii (1.1%, 1/89). The rate of single infection was 80.8% (21/26 infected fish), while 19.2% (5/26) showed mixed infection with 2 to 3 different anisakis species.
김위식 ( Wi Sik Kim ),전찬혁 ( Chan Hyeok Jeon ),김정호 ( Jeong Ho Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was evaluated to monitor infectious hematopoietic necrosis virus (IHNV) from artificially infected rainbow trout Oncorhynchus mykiss. The cumulative mortalities of fish challenged with IHNV at 106.5 TCID50/fish, 105.5 TCID50/fish and 104.5 TCID50/fish were 40%, 0% and 0%, respectively. Dead fish and survivors at 16 and 28 d post-challenge in each group were employed for IHNV detection by RT-LAMP assay and virus isolation using BF-2 cells. IHNV from 104.3 to 106.8 TCID50/㎖ was isolated from all the dead fish and also detected in all of the examined dead fish by RT-LAMP assay. In survivors at 16 d, 60% (3/5 fish, 102.8-105.05 TCID50/㎖), 20% (1/5 fish, 101.05 TCID50/㎖) and 60% (3/5 fish, 101.05-104.8 TCID50/ ㎖) were found to be IHNV-positive by virus isolation in fish challenged with IHNV at 106.5 TCID50/fish, 105.5 TCID50/fish and 104.5 TCID50/fish, respectively, while 20% (1/5 fish), 0% (0/5 fish) and 20% (1/5 fish) were IHNV-positive by RT-LAMP assay. No IHNV was detected in the survivors at 28 d and control fish. These results indicate that the RT-LAMP assay is useful for detection of IHNV in diseased fish although it is not enough to monitor virus in IHNV-survivors.