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임종득,문진균,김형섭,Lim, Jong-Deuk,Moon, Jin-Kyun,Kim, Hyung-Seop 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.1
This study was undertaken to examine the protein content of GCF and serum from noraml population in order to standardize the sample loading on SDS/PAGE gels. The resulats were as follows ; 1. The protein concentration of serum was not different between normal group and diseased group. 2. In GCF, the bands of lower molecular weight than albumin were heavily stained, but in serum, the protein bands of higher molecalar weight were found. 3. The profile of protein in normal GCF was characterized by heavily staining bands at 77, 66, 55, 26 KDa corresponding to the positions of transferrin, albumin, heavy and light chains of Ig G. Also 47, 37 KDa nonplasma proteins were found.
치주염증시 ${\gamma}-Glutamyl$ transpeptidase의 연구
이석초,임종득,유광렬,오귀옥,김형섭,Lee, Seok-Cho,Lim, Jong-Deuk,Yoo, Kang-Rgeol,Oh, Kwi-Ok,Kim, Hyung-Seop 대한치주과학회 1993 Journal of Periodontal & Implant Science Vol.23 No.3
Glutathione(GSH),a tripeptide thiol, found in virtually all cells, functions in metabolism tranasport and cellular protection. It protects cells against the destructive effects of reactive oxygen intermediates and free radicals. Also ${\gamma}-Glutamyl$ transpeptidase(${\gamma}-GTT$), an enzyme of major importance in GSH metabolism, initiates GSH degradation. In order to explore the $GSH-{\gamma}-GTT$ system as periodontal disease activity indicator, we observed the ${\gamma}-GTT$ and arachidonic acid metabolits according to clinical groups(Control, Adult periodontitis, Rapidly progressive Periodontitis). From the experiments, the following results were obtained. 1. When compared with normal, ${\gamma}-GTT$ of A. P. and R. P. P. were increased, and only the change of ${\gamma}-GTT$ of R. P. P. was statistically significant(P<0. 05). 2. The amounts of arachidonic acid metabolites were not different with statistical significance among the clinical groups. 3. ${\gamma}-GTT$ may by useful adjuncts as new cytoprotective indicator and periodontal disease activity indicator in accordance with positive corelation pocket depth, attachment level and ${\gamma}-GTT$.
임종득,김형섭 全北大學校 齒醫學硏究所 1991 전북치대논문집 Vol.9 No.1
This study was undertaken to examine the protein content of GCF and serum from normal population in order to standardise the sample loading on SDS/PAGE gels. The results were as follows : 1. The protein concentration of serum was not different between normal group and diseased group. 2. In GCF, the bands of lower molecular weight protein than albumin were heavily stained, but in serum, higher molecular weight protein bands are did. 3. The profile of protein in normal GCF was characterized by heavily staining bands at 77, 77, 55, 26 KDa corresponding to the positions of transferrin, albumin, heavy and light chains of Ig G. Also 47, 37 KDa nonplasma proteins are found.