오리에서 Duck Hepatitis Virus와 Salmonella typhimurium의 혼합 감염 증례

이혜림(Hae Rim Lee),구본상(Bon-Sang Koo),이영주(Young-Ju Lee),모인필(In Pil Mo) 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.1

2010년 2월 전남 지역의 인접한 두 곳의 오리농가에서 급성 폐사가 발생하여 병성감정을 실시한 결과, DHV와 살모넬라균의 혼합 감염으로 판정되었다. A 농장의 경우 폐사 전에 몸을 떨고 머리와 다리 부위를 뒤로 뻗치는 등 전형적인 후궁반장 등의 신경 증상을 보였으며, 부검 소견 상 총 10수 중 3수에서 간의 출혈 반점과 소장 내 출혈이 관찰되었다. 하지만 B 농장은 임상 증상이 없었으며, 또한 부검소견 상총 10수 중 2수에서 선위 궤양이 관찰된 것을 제외하고 아무런 육안 병변이 관찰되지 않았다. 간에 대한 조직학적 소견으로 두 농장에 공통적으로 간세포의 다발성 괴사 및 정상배열 소실, 담세관의 증식, 림프구의 침윤, 섬유소성 괴사 및 세균 집락이 관찰되었고 전반적인 출혈 소견이 확인되었다. 분자생물학적 검사 결과, 두 농장 모두 DHV와 살모넬라균의 유전자 양성으로 확인되었다. 본 논문은 오리에서 DHV와 S. typhimurium의 혼합 감염의 병리학적 특성을 보고하는 증례이며, 또한 국내에 A형과 C형 DVH가 동시에 존재하고 있음을 밝혔다. In January 2010, high mortalities of ducklings occurred in two adjacent farms. Ducklings in farm A showed neurologic signs including paddling, opisthotonus and lameness, but ducklings from farm B had no specific clinical signs. At post mortem, diffused hemorrhagic spots in the liver and hemorrhages in the small intestine were observed in ducklings from farm A, and ducklings from farm B had only proventricular ulceration. Microscopically, multiple necrosis of hepatocytes, hyperplasia of the bile ducts, hemorrhages, infiltration of lymphocytes and bacterial colonies were commonly observed in the liver of ducklings from both farms. Also, type A and C duck hepatitis virus (DHV) were isolated from farm A and farm B, respectively and Salmonella typhimurium was commonly isolated and identified serologically and biochemically. To our knowledge, this is the first report about the co-infection of DHV and Salmonella typhimurium in ducklings, and co-circulation of type A and C duck hepatitis viruses in Korea.

국내 산란계의 주요 바이러스성 질병에 대한 혈청학적 모니터링 결과 및 분석 (pp.361-372)

이혜림(Hae Rim Lee),김종만(Jong Man Kim),김진형(Jin Hyung Kim),김창문(Chang Moon Kim),소현희(Hyun Hee So),이동우(Dong Woo Lee),하봉도(Bong Do Ha),홍성철(Song Chol Hong),모인필(In Pil Mo) 韓國家禽學會 2010 韓國家禽學會誌 Vol.37 No.4

본 연구에서는 충북대학교 조류질병학실험실에 2009년 한 해동안 의뢰된 산란계의 혈청 검사 결과에 대해 분석하여, 산란계의 주요 질병에 대한 국내 산란계의 면역 상태 및 질병 감염 실태를 파악하였다. 검사 대상 질병은 AI, ND, IB, aMPV, EDS'76, IBD, CIA이었으며, 산란계의 성장 단계에 따라 주령 구간을 나누어 분석하였다. AI, ND, IB는 모체이행항체가 감소한 후 산란 기간에 걸쳐 혈청 역가가 안정적으로 형성되는 특징을 보여 주었다. 그러나 AI는 모든 주령 구간에 걸쳐 음성인 계군이 존재하는 반면, ND는 3~10주령 구간의 한 음성 계군을 제외하고 전 주령에 걸쳐 100%의 계군 양성률을 보이고, ND의 평균 GMT가 AI의 평균 GMT보다 높았는데, 이는 두 질병의 백신 정책의 차이에 기인한 것이다. IB의 산란기의 안정적인 역가는 백신 역가에 산란기 전반에 걸친 야외 감염 개체의 존재로 인한 야외감염 역가가 더해진 것으로 판단된다. aMPV는 2009년에 백신을 실시하지 않았던 질병이므로, 양성 역가를 통해 aMPV의 야외 감염을 추적할 수 있었으며, GMT 변화 및 계군 내양성 개체율의 증가 경향을 통해 일령이 증가할수록 야외감염률이 증가하는 양상을 파악할 수 있었다. EDS'76은 산란 기간에 걸쳐 높은 양성률과 낮은 변이계수를 보여 야외감염이 아닌 백신에 의한 역가 형성이 대부분임을 알 수 있었다. IBD의 모체이행항체는 높은 수준으로 이행되는 것을 확인하였으며, CIA는 백신을 적용하지 않은 계군에서 양성과 음성 계군이 모두 존재하였으며, 그 차이는 차단 방역에 기인하는 것으로 판단되었다. 본 연구를 통하여 국내 산란계에서의 혈청 역가 분포를 파악하는데 많은 정보를 얻을 수 있었으나, 향후 지속적인 야외 계군의 혈청학적 모니터링이 되어야 할 것으로 판단된다. Serological evaluation for the poultry is important for various reasons, such as designing and assessing the vaccination program and diagnosing diseases and for this reason, serologic tests for the layer flocks have been conducted on a regular basis. Moreover, the nationwide serological survey and analysis are essential to understand the epidemiological status of national poultry industry. In this sense, the study was conducted to evaluate the immune status of the layer flocks with the sera submitted to Avian Disease Laboratory, Chungbuk National University in 2009, and several important viral diseases were selected for evaluation including low pathogenic avian influenza (LPAI), Newcastle disease (ND), infectious bronchitis (IB) and avian metapneumovirus (aMPV). For LPAI and ND, the age-related patterns of geometric mean titer (GMT) changes were similar but there were differences in the flock positive rate and the level of GMT due to the different vaccination policy. In the case of IB, the values of GMT showed that the field infection was more prevalent than expected. For aMPV, positive birds in a flock increased as the layers got older, which reflected the course of field infection because vaccination against aMPV was not allowed in 2009. From this study, the immune status for the main viral diseases in layers became more clarified but this information was limited because of only one year study. Therefore, serological survey needs to be conducted on a yearly basis and furthermore include broilers and breeders for a better understanding of the health status in the national poultry industry.

고지방식이 마우스 비만모델에서 발효대두파우더의 체중증가량 변화와 지방간 개선

총배금,이희영,이혜림,정해경,윤연미,윤미정,박선동,신순식,Tsung, Pei-Chin,Lee, Hee-Young,Lee, Hye-Rim,Jeong, Hae-Gyeong,Yin, Yuan-Mi,Yoon, Mi-Chung,Park, Sun-Dong,Shin, Soon-Shik 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.2

Objectives : We investigated the effects of fermented soybean(FSOB) on body weight and examined whether hepatic lipid accumulation are inhibited by it in high fat diet-fed obese male mice. Methods : 8 weeks old, high fat diet-fed obese male mice were divided into 5 groups: C57BL/6N normal, control, FSOB(1), (2) and (3). After mice were treated with FSOB for 9 weeks, we measured body weight gain, food intake, feeding efficiency ratio, fat weight, plasma leptin and lipid levels. We also did histological analysis for liver and fat on the mice. Results : 1. Compared with controls, FSOB-treated mice had lower body weight gain and adipose tissue weight, the magnitudes of which were prominent in FSOB(3) and FSOB(1). 2. Compared with controls, FSOB-treated mice had lower feeding efficiency ratio and blood plasma leptin levels, the magnitude of which was prominent in FSOB(3). 3. Compared with controls, FSOB-treated mice had lower blood plasma total cholesterol and LDL-cholesterol levels. 4. Blood plasma AST and ALT concentrations were not changed by FSOB, indicating FSOB do not show any toxic effects. 5. Consistent with their effects on body weight gain, the size of adipocytes were significantly decreased by FSOB, whereas the adipocyte number per unit area was significantly increased, suggesting that FSOB decreased the number of large adipocytes. Hepatic lipid accumulation was decreased by FSOB. Conclusions : These results demonstrate that FSOB effectively reduces body weight gain, feeding efficiency ratio, blood plasma leptin level and improves hepatic lipid accumulation.

경기도 시설원예 스마트팜 빅데이터 수집 기반 구축

전명희(Myoung Hee Jeon),정구현(Gu-Hyun Jung),박인태(In-Tae Park),전혜영(Hae-Young Jeon),이혜림(Hye_Rim Lee) 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.5

고지방식이 비만마우스에서 월비가출탕(越婢加朮湯)이 식이효율과 내장지방에 미치는 영향

안정란 ( Jeong Ran An ),강연경 ( Yeon Kyeong Kang ),장동호 ( Dong Ho Chang ),이인선 ( In Seon Lee ),신순식 ( Soon Shik Shin ),정해경 ( Hae Gyeong Jeong ),이희영 ( Hee Young Lee ),이혜림 ( Hye Rim Lee ) 한방재활의학과학회 2011 한방재활의학과학회지 Vol.21 No.1

Objectives: This study was undertaken to verify the effects of Wolbigachul-tang 1(WBCEx1) on obesity using high fat diet-induced male mice and to investigate the molecular mechanisms involved. Methods: 8-week old C57BL/6 mice were divided into 5 groups; lean control, obese control, WBCEx1, 2, 3. After mice were treated with WBCE x1(water extract), 2(30% ethanol extract), 3(water extract; Ephedra sinica Stapf., Gypsum fibrosum) for 12 weeks, body weight gain, feeding efficiency ratio, plasma lipid and glucose metabolism, the messenger RNA(mRNA) expression of peroxisome proliferator activated receptor(PPAR)α target genes were measured. In addition, PPARα target gene expression was examined in liver, white adipose tissue and skeletal muscle. Results :1. WBCEx1-treated mice had significantly lower body weight gain and feeding efficiency ratio. 2. Consistent with the effects on body weight gain, WBCEx1 decreased the weights of epididymal and retroperitoneal white adipose tissue, inguinal subcutaneous adipose tissue, and brown adipose tissue. 3. WBCEx1 significantly decreased plasma triglyceride and total cholesterol levels. 4. The size of adipocytes were significantly decreased by WBCEx1, whereas the adipocyte number per unit area was increased. Hepatic lipid accumulation was decreased by WBCEx1. 5. WBCEx1 did not affect the mRNA expression of PPARα target genes in liver, adipose tissue, and skeletal muscle. 6. Plasma asparate aminotransferase(AST), alanine aminotransferase(ALT), blood urea nitrogen(BUN) and creatine concentrations were in the physiological range. Liver and kidney weights were significantly lower following WBCEx treatment compared with obese controls, indicating that WBCEx does not show any toxic effects on liver and kidney. Conclusions: These results suggest that WBCEx1-induced body weight reduction is associated with appetite control and mediated by a mechanism other than the activation of PPARα.

골파괴 병소를 동반한 Waldenstr m's Macroglobulinemia

김은숙,이혜림,정화순,김춘원,신동호,최일영,이강영 大韓血液學會 1990 大韓血液學會誌 Vol.25 No.1