Neuronal Nitric Oxide Synthase-Immunoreactive Neurons In the Hamster Visual Cortex: Lack of Co-localization with Parvalbumin

진미주,이지은,예은아,전창진,Jin Mi-Joo,Lee Jee-Eun,Ye Eun-Ah,Jeon Chang-Jin Korean Society of Life Science 2005 생명과학회지 Vol.15 No.3

산화질소(NO)와 칼슘 결합 단백질은 중추신경계의 다양한 세포들에서 나타나며, 이들은 각각 중요 신호전달 분자와 칼슘 완충 분자이다. 본 연구는 햄스터의 시각피질에서 뇌산화질소 합성효소 (nNOS)와 parvalbumin을 포함하는 뉴런들의 분포와 이들의 co-localization 양상을 면역세포화학적 기법을 이용하여 알아보았다. 햄스터 시각피질에서 parvalbumin에 대한 면역 반응성을 나타내는 뉴런들의 전체 수는 nNOS에 대한 면역 반응성을 보이는 뉴런들의 수보다 17배나 많았다. 가장 큰 차이는 시각피질 제5충에서 발견되었으며, 이곳에서 parvalbumin-면역 반응성 뉴런이 nNOS-면역 반응성 뉴런들의 수보다 54.7배나 높았다. nNOS-또는 parvalbumin-면역 반응성 뉴런들은 크기와 형태, 분포 방식이 시각피질에서 유사하게 나타났다. 그러나 이색 면역형광 기법은 햄스터 시각피질에서 nNOS와 parvalbumin을 모두 발현하는 뉴런은 없음을 보여주었다. 본 연구의 결과는 nNOS와 칼슘 결합 단백질 사이의 co-localization양상이 종간에 차이가 존재함을 나타내며 또한 시각피질에 있는 nNOS-면역 반응성 뉴런들의 다양성과 이질성뿐만 아니라 동물 다양성 이해의 중요성을 함께 제시한다고 볼 수 있다. Nitric oxide (NO) and calcium-binding proteins occur in various types of cells in the central nervous system. They are important signaling and calcium buffering molecules, respectively. In the present study, using immunocytochemistry we examined the distribution and the co-localization pattern of neurons containing neuronal nitric oxide synthase (nNOS) and parvalbumin in the visual cortex of hamster. The overall number of parvalbumin-immunoreactive (IR) neurons was 17 times higher than that of the nNOS-IR neurons in the hamster visual cortex. The highest differences were found in layer V, where parvalbumin-IR neurons were 54.7 times more abundant than nNOS-IR neurons. Many nNOS- and parvalbumin-IR neurons were similar in size, shape, and manner of distribution in the visual cortex. However, two-color immunofluorescence revealed that no neurons in the hamster visual cortex expressed both nNOS and parvalbumin. The present results indicate that there are subtle species differences in the co-localization pattern between nNOS and calcium-binding proteins. The present results also suggest not only the heterogeneity and functional diversity of nNOS-IRneurons in the visual cortex, but also the importance of understanding animal diversity

이브 생 로랑 디자인에 표현된 아트 인스피레이션 -오트 쿠튀르 컬렉션을 중심으로-

이예은 ( Ye Eun Lee ),조규화 ( Kyu Hwa Cho ) 한국패션비즈니스학회 2010 패션 비즈니스 Vol.14 No.1

This study is a part of the integrated investigation of the design of Yves Saint Laurent, and aims at examining the background of the appearance of art inspiration which the artistic quality and originality are particularly seen. And also, I would like to examine the source of the power that transcends periods, dealing with issues in the design. From the mid 1960 to 1970, Yves Saint Laurent who leaded the global fashion trend, displayed the Art Inspiration works with the innovation of fashion in Paris, rediscovery of the trends and colors in Modern Art, and background of Entourage and so forth. He brought in the various artistic genres to the fashion, and his Haute Couture collections which were created by the passion for the movie and theater art, expressed the freedom through his strong identity in the Modern Art & Culture and the atmosphere of that period. Eventually his works are reappeared transcending the generations as the fundamental power of Modernity, through the restricted line of his own, strong contrast of colors, and insight for the future.

LPS로 유도된 RAW264.7세포주에서 황금뿌리 물추출물의 항염증활성

이예은(Ye Eun Lee),박홍진(Hong Jin Park),박충범(Chung-berm Park),황승미(Seung-mi Hwang) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.2

Scutellaria baicalensis water extract (SWE)는 지질 다당류 LPS로 유도된 RAW 264.7 세포에서 NO 및 전 염증성 사이토카인인 TNF-α의 생성을 세포 독성을 유발하지 않고 유의하게 억제하였다. 또한, SWE는 iNOS 및 COX-2의 단백질발현을 농도의존적으로 감소시켰으며, ERK, JNK, p38과 같은 MAPKs 계열의 인산화발현 수준을 조사한 결과 JNK와 p38의 발현 수준을 감소시켰다. 이는 SWE가 p38 인산화를 억제함으로써 iNOS, COX, 그리고 TNF-α와 같은 전 염증성 사이토카인의 발현을 감소시키며 결론적으로 NO의 생성을 억제시킨다는 결과를 도출할 수 있었다. 본 연구는 항염증 효능 검증뿐 아니라 염증대사기전의 주요인자를 탐색함으로써 황금의 기능성 소재로써의 가능성을 시사한다. Scutellaria baicalensis has been used as a traditional medicine for diarrhea, dysentery, hypertension, hemorrhaging, insomnia, inflammation, and respiratory infections. This study examined the anti-inflammatory effect of Scutellaria baicalensis water extract (SWE) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. To evaluate the anti-inflammatory effect of SWE, RAW 264.7 macrophages were stimulated with LPS to induce the production of inflammation-related factors, which were measured by western blotting. In RAW 264.7 cells, SWE inhibited the production of nitric oxide (NO) without causing cell toxicity. SWE also reduced the expression of inducible NO synthase and cyclooxygenase-2 protein, as well as the production of pro-inflammatory cytokines (such as tumor necrosis factor-α). The phosphorylation levels of the mitogen-activated protein kinase family members, such as JNK and p38, were also reduced by SWE. Thus, SWE could be used as a potential anti-inflammatory agent.

포스트휴머니즘 연극에 드러난 실존적 인간의 인식 변화 -프란츠 카프카 원작, 히라타 오리자 각색/연출, 로봇 연극 <변신>을 중심으로

이예은 ( Lee Ye-eun ) 한국연극교육학회 2018 연극교육연구 Vol.33 No.-

This thesis explores the meaning of existence revealed in Hirata Oriza's play < Metamorphosis > adapted from Franz Kafka's, which applies the motif of metamorphosis to robots instead of worms. In order to approach the meaning of the human being in existence presented in the original and the play, two topics that I have focused on are death and art. In the original work art is the only exit to existential awareness that holds the time of life and death is presented as a pathway of salvation where such being ultimately comes to pass. The play < Metamorphosis > presents a collective death by staging a robot equally endowed with the limits of human beings and blurring the distinction between life and death. Thus the death presented as an event that causes the encounter with the existential self in the original work is reexamined as vanity, and Gregor, who is saved by death, is reconsidered as the despair of those who can not die. Even the art that enabled existential awareness in the original work is presented as a state of ‘phantom limbs’ that exists somewhere but can not know its residence. This play reveals the modesty of despair, which can not die even in its existential state, and of desiring the unknown world through the art of poetry, suggesting another point against the Cartesian human concept in the post-humanism perspective.

벌채 산물 수집이 산불연료에 미치는 영향

이예은(Ye-eun Lee),이선주(Sun Joo Lee),권춘근(Chun Geun Kwon),서경원(Kyung Won Seo),방철안(Cheol An Bang),김성용(Sung Yong Kim) 위기관리 이론과 실천 2020 Crisisonomy Vol.16 No.10

본 연구에서는 산불피해지 임분에서 산림조사를 수행하여 숲가꾸기 산물의 존치여부가 산불연료및 산불행동에 어떠한 영향을 미치는지 관찰하였다. 연구결과에 따르면, 벌채 산물을 포함한 연구대상지의 지표층 연료량은 약 38.92 ton/ha로 추정되었으며, 이는 우리나라 소나무림의 평균 지표층연료량인 약 14.46 ton/ha와 비교하였을 때 2.7배 이상 많은 수치였다. 지표화 강도를 추정한 결과, 벌채 산물을 포함했을 때는 약 8,956.33 kW/m, 제외했을 때는 약 4,454.95 kW/m로 추정되어, 벌채산물로 인해 지표화 강도가 2배 이상 높아진 것으로 나타났다. 수관화의 화염강도를 추정하기 위해서산 산불피해지와 군위산불피해지의 연소 후 남은 가지직경을 비교한 결과 군위산불은 약 0.75cm, 서산산불은 약 0.33cm로 측정되어 서산산불의 화염강도가 상대적으로 낮았을 것으로 판단했다. 본연구의 결과는 벌채 산물이 산불연료에 미치는 영향을 정량적인 결과로 제시하는 데 도움이 될 것이다. Thinning is known as an important method not only for promoting healthy forest but for preventing crown fire transition and spread. However, if you do not collect thinning slash, it may serve as a factor in the spread of forest fires. In this study, we attempted to observe the effects of thinning slash on fire behavior. The results of this study showed that total surface-fuel loads including thinning slash were estimated approximately 38.92 ton/ha in the study area, which was about 2.7 times more than that of Ⅳ Pinus densiflora stand in South Korea. Surface fire intensity (SFI) was measured at 8,956.33 kW/m with thinning slash, but 4,454.95 kW/m without thinning slash, which indicates a significant effect of thinning slash on SFI. The estimation of passive crown fire intensity showed that the fire spread rate was calculated as 0.4 km/h in Gunwi but calculated as 0.06 km/h in the study area. The results from this study could serve as a good example to explain the effects of thinning slash on wildfire fuel type.

증강현실 지리 위치 정보를 활용한 정보 공유 모바일 앱

강예은(Ye Eun Kang),장서연(Seo Yeon Jang),김대호(Dea Ho Kim),이혜란(Hye Ran Lee),이준표(Jun Pyo Lee) 한국컴퓨터정보학회 2020 한국컴퓨터정보학회 학술발표논문집 Vol.28 No.1

Increased Pro-Inflammatory T Cells, Senescent T Cells, and Immune-Check Point Molecules in the Placentas of Patients With Gestational Diabetes Mellitus

강예은,Yi Hyon-Seung,Yeo Min-Kyung,Kim Jung Tae,Park Danbit,Jung Yewon,Kim Ok Soon,이성은,Kim Ji Min,Joung Kyong Hye,Lee Ju Hee,Ku Bon Jeong,Lee Mina,Kim Hyun Jin 대한의학회 2022 Journal of Korean medical science Vol.37 No.48

Background: Gestational diabetes mellitus (GDM) is the most common metabolic complication of pregnancy. To define the altered pathway in GDM placenta, we investigated the transcriptomic profiles from human placenta between GDM and controls. Methods: Clinical parameters and postpartum complications were reviewed in all participants. Differentially expressed canonical pathways were analyzed between the GDM and control groups based on transcriptomic analysis. CD4+ T, CD8+ T, and senescent T cell subsets were determined by flow cytometry based on staining for specific intracellular cytokines. Results: Gene ontology analysis revealed that the placenta of GDM revealed upregulation of diverse mitochondria or DNA replication related pathways and downregulation of T-cell immunity related pathways. The maternal placenta of the GDM group had a higher proportion of CD4+ T and CD8+ T cells than the control group. Interestingly, senescent CD4+ T cells tended to increase and CD8+ T cells were significantly increased in GDM compared to controls, along with increased programmed cell death-1 (CD274+ ) expression. Programmed death-ligand 1 expression in syncytotrophoblasts was also significantly increased in patients with GDM. Conclusion: This study demonstrated increased proinflammatory T cells, senescent T cells and immune-check point molecules in GDM placentas, suggesting that changes in senescent T cells and immune-escape signaling might be related to the pathophysiology of GDM.

1인 가구를 위한 공동구매 및 정보 공유 커뮤니티 서비스

이예은(Yeeun Lee),김규리(Gyuree Kim),이해서(Haeseo Lee),정은혜(Eun-Hye Jeong),안형태(Hyeongtae Ahn),오병우(Byoung-Woo Oh) 한국정보기술학회 2022 Proceedings of KIIT Conference Vol.2022 No.6

국내 1인 가구의 비중이 커지면서 이와 관련된 맞춤 서비스의 수요 또한 증가하고 있다. 이러한 사회적 흐름에 주목하여 1인 가구 관련 서비스가 개발되고 있다. 그러나 사용자의 요구를 종합하여 다양한 기능을 제공하는 시스템을 찾기는 어렵다. 현 상황을 고려하여 본 논문에서는 1인 가구를 위한 맞춤형 커뮤니티 서비스를 제안한다. 본 논문에서 제안하는 서비스는 사용자의 경제적 부담을 줄이기 위해 배달 및 배송 공동구매와 OTT 플랫폼 공유 구독자 모집을 지원한다. 그리고 1인 가구 정책 및 유용한 생활 정보를 공유할 수 있다. As the proportion of single-person households in Korea increases, the demand for customized services related to this is also increasing. Paying attention to these social trends, services related to single-person households are being developed. However, it is difficult to find a system that provides various functions by synthesizing user needs. Considering the current situation, this paper proposes a customized community service for single-person households. The service proposed in this paper supports the group purchase of delivery and delivery and the recruitment of OTT platform sharing subscribers to reduce the economic burden of users. You can also share single-person household policies and useful life information.

5G 특화망 트래픽 특성 분석

이예은(Lee Ye Eun),권혜연(Kwon Hye Yeon),김기일(Kim Ki Il) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.6

메타버스 중심의 미래교육센터 구축 및 운영 방안

이예은(Yeaeun Lee),김성백(Seong Baeg Kim) 한국컴퓨터교육학회 2021 한국컴퓨터교육학회 학술발표대회논문집 Vol.25 No.2(A)

최근 코로나19로 인한 학교 교육 현장에서의 원격 수업 확대와 교육 운영 방식의 변화에 따라 예비교원에게도 원격 수업 역량 확보가 필요하게 되었다. 교육부는 교원양성대학으로서 예비교원의 원격교육 등 미래교육을 강화하기 위한 미래교육센터를 구축하는 ‘교원양성대학 원격교육 역량강화 사업’을 추진하였다. 본 연구는 예비교원이 원격 수업 실습과 온라인 콘텐츠 제작, 교육용 운영체제(플랫폼), 메타버스 기반 첨단 테크놀로지를 활용할 수 있는 공간인 미래교육센터 구축 모델을 제시하고, 해당 공간을 이용하여 원격수업 역량을 갖출 수 있도록 다양한 프로그램 운영 방안을 제시하고자 한다.