四君子湯이 免疫機能에 미치는 影響

이상현,정명,임규상,윤용갑,Lee, Sang-Hyun,Jung, Myung,Lim, Kyu-Sang,Yun, Yong-Gab 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.3

Objectives : The extract of Sagunja-tang has been traditionally used for restorative treatment of constitutional weakness, vascular and immune disorder, and nervous disease in Oriental country. This study investigated the regulatory effects of Sagunja-tang on the expression, production, and activity of immune mediators.Methods : In this study, the extract of Sagunja-tang was prepared by extracting with distilled water at 100$^{\circ}C$ for 2.5h. The extract was freeze-dried following filtration through 0.45${{\mu}m}$ filter. The extract was dissolved in Hank's balanced salt solution (HBSS) and filtered again through 0.45${{\mu}m}$ filter before use. The level of nitrite, an oxidative product of nitric oxide(NO) was measured in the culture medium by the Griess reaction. The levels of prostaglandin E₂(PGE₂), Th1 cytokines (IFN-${\gamma}$, IL-2) and Th2 cytokines(IL-4, IL-5, IL-13) were measured by enzyme-linked immunosorbent assay and the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were determined by Western blot analysis. Also examined the effects of the extract on T-cell proliferation and cytotoxic activity of natural killer cells.Results : In this investigation, Production levels of Th2 cytokines (IL-4, IL-5, IL-13) was inhibited in a dose dependent manner by treatment with the extract. I also found that the extract increased T-cell proliferation and cytotoxic activity of natural killer cells in a dose-dependent manner.Conculsions : These results suggest that the water extract of Sagunja-tang may be useful for a therapeutic drug against a sickly constitution and immune diseases, probably by regulating the production of immune mediators.

고속액체크로마토그라피에 의한 현삼근(玄蔘根) 중 p-Methoxycinnamic acid의 정량

이상현,지형준,신국현,Lee, Sang-Hyun,Chi, Hyung-Joon,Shin, Kuk-Hyun 한국생약학회 1999 생약학회지 Vol.30 No.3

A new method for quantitative determination of p-methoxycinnamic acid in Scrophulariae radix by high performance liquid chromatography was established. A reversed-phase system with SPHERI-5 RP-18 5 micron $(250\;{\times}\;4.6\;mm)$ column using TFA (0.05%) and acetonitrile as a mobile phase was developed. p-Methoxycinnamic acid and N-(p-methoxycinnamoyl)-p-aminophenol as an internal reference were detected at 310 nm and the analysis was successfully carried out within 50 min.

농업용 드론의 배터리 셀 밸런싱을 위한 퍼지제어기 개발

이상현,Lee, Sang-Hyun 한국인터넷방송통신학회 2017 한국인터넷방송통신학회 논문지 Vol.17 No.5

리튬 폴리머 배터리는 높은 안전성, 빠른 충전 및 긴 라이프 사이클 등으로 인해 에너지 저장치(ESS: Energy Storage System), 전기자동차(EVs: Electric Vehicles)등에 채택이 되어 사용되고 있으며, 그리고 현재는 농업용 드론에서 까지 사용이 되고 있다. 그러나 리튬 폴리머 배터리는 과충 방전에는 리튬-이온 배터리 내의 격차구조가 파괴되어 배터리 수명이 줄어들게 되며, 과충 방전을 방지하기 위해 불균등한 셀 전압을 균등 제어 할 수 있는 셀 밸런싱 시스템이 필수적이다. 본 논문은 각 셀의 충 방전할때의 전압차이를 검출하여 불균형된 셀을 확인하여 비선형 시스템에 적합한 퍼지 제어기를 개발하여 적용한 셀별 밸런싱 알고리즘을 제안한다. 본 논문은 농업용 드론의 배터리팩의 셀 밸런싱을 퍼지제어를 하여 셀 간 균등 제어를 위해 설계하였으며, 최종 결과로 셀 간 밸런싱이 잘 되는지 확인하고 자 셀이 2개 있을 때와 6개 그리고 최종적으로 12개의 각 셀 밸런싱이 되는지를 확인하였다. 이는 다른 제품에도 사용할 수 있는지를 실험하고자 하였으며, 확인결과 사용된 셀의 개수와는 관계없이 셀별 밸런싱이 잘 되고 있음을 확인하였다. Lithium polymer batteries are used in energy storage systems (ESS), electric vehicles (EVs), etc. due to their high safety, fast charging and long lifecycle, and now they are used in agricultural drones. However, when overcharging and overdischarging, the lithium-polymer battery is destroyed in the gap structure in the lithium-ion battery and the battery life is reduced. In order to prevent overcharge and overdischarge, uneven cell voltage Cell balancing system is needed. In this paper, a fuzzy controller suitable for nonlinear systems is proposed by detecting the unbalanced cells by detecting the voltage difference between charging and discharging of each cell, and suggesting the applied cell balancing algorithm. In this paper, we have designed the cell balancing of the battery pack of agricultural drones by fuzzy control and it is designed for equal control between cells. As a final result, we checked whether cell balancing is good, and when there are two cells, Cell balancing was confirmed. We tested whether it could be used for other products. As a result, we confirmed that cell balancing is good regardless of the number of cells used.

Antitumor and Immunostimulating Activities of Acanthopanax sessiliflorus Fruits

이상현,Yeon Sil Lee,Sang Hoon Jung,Jun Ji,Kuk Hyun Shin,Bak-Kwang Kim,강삼식 한국생약학회 2003 Natural Product Sciences Vol.9 No.2

The antitumor and immunostimulating activities of Acanthopanax sessiliflorus fruits were investigated. Polysaccharide isolated from this plant, when administered consecutively for 9 days at 50 and 100 mg/kg i.p. in mice, caused a significant increase in the life span and a significant decrease in the tumor weight and volume in mice inoculated with Sarcoma-180 tumor cells. Polysaccharide was also demonstrated to exhibit phagocytosis-enhancing activity as measured by the carbon clearance in mice. Polysaccharide, when administered i.p. at 50 and 100 mg/kg/day for 3 consecutive days, exhibited a significant RCtr/RCc [the rate of regression coefficient of the animals teated (RCtr) to that of the control (RCc)], being 1.44 (PI = 1), 1.52 (PI = 2) which was approximately the same with that of enhancement of phagocytosis, its potency as expressed by the regression coefficient ratio of zymosan (RCtr/RCc = 1.55, PI = 2), a typical phagocytosis enhancer. Polysaccharide also caused a significant increase in the acid phosphatase activity representing lysosomal enzymes in macrophages at 1-100 ig/ml in vitro in compliance with in vivo results. These results suggest that the antitumor activity of polysaccharide might be related to the immunostimulating function.

IoT 보안을 위한 SHA-256 해시 프로세서의 면적 효율적인 설계

이상현,신경욱,Lee, Sang-Hyun,Shin, Kyung-Wook 한국정보통신학회 2018 한국정보통신학회논문지 Vol.22 No.1

전자서명, 인증 코드, 키 생성 알고리듬 등의 보안 프로토콜에 사용되는 SHA-256 해시 함수를 면적 효율적으로 설계하였다. 설계된 SHA-256 해시 프로세서는 입력 메시지에 대한 패딩 및 파싱 기능을 수행하는 패더 블록을 포함하여 프리프로세싱을 위한 소프트웨어 없이 동작하도록 구현하였다. 라운드 연산을 16-비트 데이터 패스로 구현하여 64 라운드 연산이 128 클록 주기에 처리되도록 하였으며, 이를 통해 저면적 구현과 함께 성능 대비 하드웨어 복잡도 (area per throughput; APT)를 최적화 하였다. 설계된 SHA-256 해시 프로세서는 Virtex5 FPGA로 구현하여 정상 동작함을 확인하였으며, 최대 116 MHz 클록 주파수로 동작하여 337 Mbps의 성능을 갖는 것으로 평가되었다. ASIC 구현을 위해 $0.18-{\mu}m$ CMOS 셀 라이브러리로 합성한 결과, 13,251 GE로 구현되었으며, 최대 동작주파수는 200 MHz로 예측되었다. This paper describes an area-efficient design of SHA-256 hash function that is widely used in various security protocols including digital signature, authentication code, key generation. The SHA-256 hash processor includes a padder block for padding and parsing input message, so that it can operate without software for preprocessing. Round function was designed with a 16-bit data-path that processed 64 round computations in 128 clock cycles, resulting in an optimized area per throughput (APT) performance as well as small area implementation. The SHA-256 hash processor was verified by FPGA implementation using Virtex5 device, and it was estimated that the throughput was 337 Mbps at maximum clock frequency of 116 MHz. The synthesis for ASIC implementation using a $0.18-{\mu}m$ CMOS cell library shows that it has 13,251 gate equivalents (GEs) and it can operate up to 200 MHz clock frequency.

Determination of Fucosterol in the Marine Algae Pelvetia siliquosa by Gas Chromatography

이상현,이연실,Sang Hoon Jung,Hye Seung Lee,Sam sik Kang,Kuk Hyun Shin 한국생약학회 2004 Natural Product Sciences Vol.10 No.1

A new method for the quantitative determination of fucosterol in the marine algae Pelvetia siliquosa by gas chromatography was established. A HP-1 column programmed as 200oC (5 min) → 5/min → 280oC (10min) was developed. The analysis of fucosterol in P. siliquosa was successfully carried out within 30 min.

Sol-gel법에 의한 박막태양전지용 CuInS₂ 박막의 증착과 특성

이상현,이승엽,박병옥,Lee, Sang-Hyun,Lee, Seung-Yup,Park, Byung-Ok 한국결정성장학회 2011 한국결정성장학회지 Vol.21 No.4

박막 태양전지의 저가 고효율화를 실현하기 위해 넓은 면적의 기판 위에 코팅이 가능하며 진공의 유자가 필요 없기 때문에 장치가 간단하고 고순도의 균질한 박막을 얻을 수 있고 박막의 조성을 쉽게 조절할 수 있는 Sol-Gel법을 이용 하였다. Se보다 저가이며 독성이 없고 풍부한 원료인 S로 치환하여 사용하며 Cu/In비 값을 조절하고 tetragonal chalcopyrite $CuInS_2$의 열처리 온도에 따른 박막의 구조적, 광학적 특성에 미치는 변수들의 영향을 알아보았다. XRD pattern을 관찰한 결과 Cu/In비가 1.0일 때 $2{\theta}=27.9^{\circ}$에서 주피크가 가장 강하게 나타났으며 (112) 방향의 배향성을 가진 chalcopyrite상임을 확언 할 수 있었다. 열처리 온도가 증가할수록 (112) 면의 강도가 커지며 $500^{\circ}C$에서 열처리를 한 $CuInS_2$ 박막은 tetragonal 구조의 화학량론적 $CuInS_2$ 특징을 나타내고 본 실험의 샘플의 격자상수를 측정한 값이 a = 5.5032, c = 11.1064 ${\AA}$이며 JCPDS(Joint Committee on Powder Diffraction Standards)에 보고된 데이터 a = 5.523, c = 11.14 ${\AA}$과 거의 일치하였다. 광학적 특성을 알아보기 위해 측정한 광투과율은 가시광선 영역(380~770 nm)에서 전체적으로 30% 이하로 나타났다. $CuInS_2$ thin films were prepared using a sol-gel spin-coating method. That makes large scale substrate coating, simple equipment, easy composition control available. The structural and optical properties of $CuInS_2$ thin films that include less toxic materials (S) instead of Se, tetragonal chalcopyrite structure. Copper acetate monohydrate ($Cu(CH_3COO)_2{\cdot}H2O$) and indium acetate ($In(CH_3COO)_3$) were dissolved into 2-propanol and l-propanol, respectively. The two solutions were mixed into a starting solution. The solution was dropped onto glass substrate, rotated at 3000 rpm, and dried at $300^{\circ}C$ for Cu-In as-grown films. The as-grown films were sulfurized inside a graphite container box and chalcopyrite phase of $CuInS_2$ was observed. To determine the optical properties measured optical transmittance of visible light region (380~770 nm) were less than 30 % in the overall. The XRD pattern shows that main peak was observed at Cu/In ratio 1.0 and its orientation was (112). As annealing temperature increases, the intensity of (112) plane increases. The unit cell constant are a = 5.5032 and c = 11.1064 $\AA$, and this was well matched with JCPDS card. The optical transmittance of visible region was below than 30 %.

콘크리트의 탄산화 관점에서 CO₂ 배출량-흡수량 평가에 관한 연구

이상현,이성복,이한승,Lee, Sang-Hyun,Lee, Sung-Bok,Lee, Han-Seung 한국콘크리트학회 2009 콘크리트학회논문집 Vol.21 No.1

콘크리트는 생산과정에서 다량의 이산화탄소를 배출하는 시멘트를 사용하기 때문에 반친환경적 재료로 인식되고 있다. 하지만 콘크리트는 사용기간 중 탄산화 과정을 통하여 대기중의 이산화탄소를 흡수한다. 이에 본 연구에서는 기존문헌 고찰을 통하여 1) 콘크리트 내 탄산화 가능한 물질의 농도, 2) 탄산화된 콘크리트의 체적, 3) 이산화탄소 분자량을 이용, 탄산화를 통한 콘크리트의 이산화탄소 흡수량의 정량적 산출 방법을 제시하였다. 또한 콘크리트 생산에 사용되는 재료들의 이산화탄소 배출량 자료를 이용하여 단위 콘크리트 생산에 따른 이산화탄소 배출량을 정량적으로 산출하였다. 이러한 콘크리트의 이산화탄소 흡수량 및 배출량의 정량적 산출방법을 이용하여 실제 사용중인 아파트 건축물 1동을 대상으로 하여 콘크리트의 생산에 따른 배출량과 사용기간에 따른 이산화탄소 흡수량을 정량적으로 산출하여 이산화탄소의 배출량-흡수량 평가를 실시하였다. 그 결과 건축물을 40, 60, 80년 사용시, 사용된 콘크리트의 이산화탄소 배출량 대 흡수량의 비율이 3.65, 4.47, 5.18%로 나타났다. 본 연구는 콘크리트 생산 및 사용에 따른 이산화탄소 배출량-흡수량의 정량적 산정방법에 연구의 목적을 두었으며 이산화탄소 배출량-흡수량 평가 결과 구조물을 80년 사용할 시 약 5.18%로 그 값이 미비하였으나 시멘트의 혼화재 치환율 증가를 통한 배출량 저감과 탄산화 체적 증가를 통한 이산화탄소 흡수량 증가를 통해 배출량-흡수량을 향상시킬 수 있으며, 향후 콘크리트의 이산화탄소 배출량-흡수량 평가에 본 연구의 방법이 유용하게 활용될 수 있을 것으로 판단된다. A concrete is considered unfriendly-environmental material because it uses cement which emits much $CO_2$ during producing process. However, a concrete absorbs $CO_2$ through carbonation process during service life. In this paper how much concrete absorbs $CO_2$ through carbonation was calculated using 1) concentration of carbonatable substances in concrete, 2) carbonated volume of concrete, 3) molecular weight of $CO_2$ based on references and the method was proposed. $CO_2$ emission from producing $1m^3$ concrete was calculated based on $CO_2$ emission datum of materials used in concrete. From using these methods that calculate $CO_2$ emission and absorption of concrete, assessment of $CO_2$ emission-absorption against a real apartment was conducted by subtracting absorption $CO_2$ according to service life from $CO_2$ emission in the process of making concrete. As a result, a ratio of absorption over emission of $CO_2$ through concrete carbonation according to service life 40, 60, 80 years was assessed about 3.65, 4.47, 5.18%. An objective of this study is to propose how to calculate emission - absorption of $CO_2$ from producing and using concrete. Although the result value, emission - absorption of $CO_2$, is 5.18% very low when the service life of an apartment is 80years, the value can be improved by reducing emission from using blended cement such as blast furnace slag or increasing replacement ratio of cement and increasing carbonated volume of concrete from expanding service life of a building. This study may be useful when $CO_2$ emission - absorption of concrete is evaluated in the further study.

쌀 생산 및 소비에 따른 지역 간 청색 가상수 흐름 추정을 통한 지역 수자원의 기여도 분석

이상현,최진용,유승환,김윤형,Lee, Sang-Hyun,Choi, Jin-Yong,Yoo, Seung-Hwan,Kim, Yoon Hyung 한국농공학회 2016 한국농공학회논문집 Vol.58 No.1

The aim of this study is to analyze the contribution of regional water resources through the gap between water used for rice production and water used for consumption. The blue water use for rice production and for consumption was quantified and the regional blue water flows were estimated using the virtual water concept from 1995 to 2010. About $1134.4Mm^3/yr$ of blue water flowed among the provinces and metropolises of Korea, and about 28.5 % of total blue water flows came from Jeonnam province. In addition, blue water usage for rice was classified into three categories: water for production, internal consumption, and overproduction in each region. In Jeonnam, $633.8Mm^3/yr$ of blue water totally used for rice production, and 50.9 % and 15.5 % were used for external and internal rice consumption, respectively. The other 33.6 % was used for over production of rice for food security. This study assumed the blue water flows depended on the gap between virtual water use for rice production and consumption. However, the analysis of regional blue water usage and flows might show the importance of other region's water resources, and make policy decision-makers aware of the integrated water management among the regions.

구형지하공동 형성이 지표침하에 미치는 영향에 대한 3차원 개별요소해석

이상현,이항로,송기일,Lee, Sang-Hyun,Lee, Hang-Lo,Song, Ki-Il 한국터널지하공간학회 2016 한국터널지하공간학회논문집 Vol.18 No.2

The underground cavity known as one of the reasons of ground surface settlement is a discontinuous character. Therefore, it is limited to analyze with continuum analysis. In this research, The spherical underground cavity affecting the ground surface settlement is studied with Discrete Element Method. Ground properties, depth and diameter of the spherical underground cavity are chosen as factors of the spherical underground cavity and the effect of the each factor variations on the ground surface settlement is analyzed. Relative depth to the diameter of the spherical underground cavity is also studied. The result of the research suggests the basis of underground cavity collapse prediction and standard of support. 지표침하를 발생시키는 다양한 원인 중에 하나로 알려진 지하에서 발생되는 공동은 지반의 불연속적 특징이다. 그러므로 기존의 연속체해석으로 분석하기에는 한계가 있을 것으로 판단된다. 본 연구에서는 지표침하를 발생시키는 구형지하공동에 대해서 개별요소법을 활용하여 분석하였다. 지표침하에 영향을 미치는 구형지하공동의 특성인자로서 지반의 물성치, 구형지하공동의 심도 및 크기를 선택하였고, 각 특성인자들의 값의 변화가 지표침하에 미치는 영향에 대해 분석하였다. 또한 지하공동의 직경에 대한 상대적 깊이와 지표침하의 관계를 분석하였고, 이를 통해 지하공동의 붕괴 예측 및 보강유무를 결정하기 위한 근간을 제시하였다.