EDTA, 염산 테트라싸이클린, 구연산 처치가 치근면에 미치는 영향

안성희,채중규,김종관,조규성,Ahn, Seong-Hee,Chai, Jung-Kiu,Kim, Chong-Kwan,Cho, Kyoo-Sung 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.3

The goal of periodontal therapy is the periodontal regeneration by the removal of microorganisms and their toxic products from the periodontally diseased root surface. To achieve periodontal regeneration, root conditioning as an adjunct to root planing has been done. There are low pH etchants such as citric acid, tetracycline-HCl, and EDTA solution which is a neutral chelating agent. The purpose of present study was to examine the effect of root conditioning by citric acid, tetracycline HCl, and EDTA. Total 35 root specimens(6${\times}$3${\times}$2mm) were prepared from the periodontally diseased teeth, scaled and root planed. The specimens were treated with normal saline for 1 minute, saturated citric acid(pH 1) for 3 minutes, 50mg/ml tetracycline-HCl(pH 2) for 5 minutes, 15% EDTA(pH 7) for 5 minutes using rubbing technique. The specimens were examined under scanning electron microscopy at 1000, and 3000 magnification. On the microphotographs taken at 1000 magnification, the numbers of opened and patent dentinal tubules per unit area(10,640${\mu}m^2$) were counted. And the diameters of opened dentinal tubules per unit are (10,640${\mu}m^2$) were measured. The differences of number and diameter among all groups were statistically analyzed by Kruskal Wallis Test. The results were as follows; 1. In the specimens applied with normal saline(control group), the root surface was finely cracked, and was covered by irregular smear layer. Neither exposed dentinal tubules nor any patent dentinal tubules could be seen. 2. In the specimens applied with saturated citric acid(experimental 1 group), the globular collagen fibers were exposed around the peritubular space, and many dentinal tubules were revealed. 3. In the specimens applied with tetracycline-HCl(experimental 2 group), the process-like collagen fibers were exposed around the peritubular space, and some dentinal tubules were revealed. 4. In the specimens applied with 15% EDTA(experimental 3 group), the root surface was covered by the collagenous fibrillar network, and many dentinal tubules were revealed. 5. The numbers of opened and patent dentinal tubules were significantly more in exp. 1 group and exp. 3 group than in exp. 2 group(P<0.05). But there was no significant difference between exp. 1 group and exp. 3 group. In control group, the number of opened and patent dentinal tubules could not be counted because any dentinal tubules couldn't be seen. 6 . The diameter of opened dentinal tubules was significantly smaller in exp. 1 group and exp. 3 group than in exp. 2 group(P<0.05). But there was no significant difference between exp. 1 group and exp. 3 group. In control group, the diameter of opened dentinal tubules could not be measured because any dentinal tubules couldn't be seen. The results demonstrate that root conditioning with citric acid, tetracycline- HCl, and EDTA is more effective in periodontal healing than only root planing, and 15% EDTA solution can replace low pH etching agents such as citric acid, tetracycline-HCl for root conditioning.

IMF 관리체제 이후 임차가구의 주택소비 행태변화

하성규(Seong Kyu Ha),안성희(Sheong Hee Ahn) 한국사회정책학회 1999 한국사회정책 Vol.6 No.1

본 연구는 IMF 구제금융체제 이후 전세 임차가구의 주택소비행태를 파악하는 것이 목적이다. IMF 관리체제는 저소득 전세가구에게 다양한 사회·경제적인 충격을 가져왔다. 본 연구에서는 전세 임차가구의 사용주거면적, 소득, 전세보증금 등 주택소비 행태가 IMF 전후 어떻게 변화하였는가를 비교·분석하고자 한다. 조사대상지역은 주택소유율이 낮은 서울시 관악구이며 면접조사를 통해 자료를 구득하였다. 조사결과 IMF 영향으로 전세 임차가구의 소득이 감소하였으며, 이로 인해 주택소비패턴의 변화가 발견되었다. IMF 이후 전세가구들의 주택 전용면적은 이사오기 전 주택 전용평수보다 줄어들었다. 전세수요가 감소함에 따라 전세금은 하락하였고, 이에 따라 낮은 전세보증금으로도 동일한 전세주택을 임차할 수 있었다. 그러나 전세금 반환문제로 집주인과 임차인간의 갈등이 심화되는 현상이 나타났다. 그리고 전세가구들은 소득의 감소에 따라 소비지출 부문 중 큰 비중을 차지하는 주택부문에 대한 지출을 줄이고자 하는 경향을 명확히 보여주었다. 소득의 감소로 인한 임차가가구들의 주택소비 하향추세는 일반적인 경향이며, 이로 인해 저소득층의 주거수준 악화와 주거불안정이 사회문제로 부각되고 있다. 특히 저소득 임차가구의 최소한의 인간다운 주거생활 보장을 위해 다양한 주택정책프로그램의 개발이 요구된다. 이들 임차가구를 위해 저리 전세금의 융자는 물론 주택보조금 제도, 임대료 보조제도 등의 실시가 요망된다. 주거복지제도의 전반적 변화가 필요한 시기이다. Korea, Inc. has been a marvel of developing nations. With economic growth rate of almost ten percent per year for the past three decades, it has been the envy of the developing nations. Then something went horribly wrong. The Korean miracle stopped working and an IMF bailout was required. Although the Koreans popped the champagne bottles too early, they certainly deserved to toast their achievements, given their hard work for the past 30 years. The purpose of this paper is to analyse the changes of housing consumption pattern of Chonsei tenants before and after the IMF crisis. For this study, at the first step, we examine the characteristics of Chonsei tenants in rental housing market. Second, we explored general impacts on housing markets and rental sector which has been caused by the IMF crisis. Third, we analyzed tenants` behavior in the private rental and characteristics of housing consumption through the questionnaire method. We selected Kwan-Ak Gu as a survey area where has high ratio of Chonsei households than any other areas in Seoul. A nationwide income`s decrease resulted in tenants` income and it made housing consumption to be decreased. In respect of housing size, this was caused 75% of tenants to decrease from 25.17 pyong to 21.30 pyong per household. Also the numbers of room were decreased from 2.62 to 2.48 per households. Many tenants cannot secure minimum adequate housing without excessive payments being exacted from their meager incomes or no income. The most needy households should be accorded first priority in the distribution of housing subsidies. Governments should consider the introduction of housing allowance system in order to relive the burdensome cost of shelter on poor tenants.

임신성 내당능장애 및 임신성 당뇨를 동반한 임신의 양상 및 주산기 예후

이원종(Won Jong Lee),안성희(Seong Hee Ahn),김행수(Haeng Soo Kim),양정인(Jeong In Yang),김윤석(Yoon Seok Kim),오준환(Joon Hwan Oh),한기수(Ki Su Han),오기석(Kie Suk Oh) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.6

N/A Objective : To evaluate the clinical manifestations and perinatal outcomes of pregnancies complicated with gestational impaired glucose tolerance (GIGT) and gestational diabetes mellitus (GDM). Methods : We performed 50gm oral glucose tolerance test (OGTT) for GIGT and GDM screening in 4,367 pregnant women at 24-28 weeks of gestation. In 1,010 women with plasma glucose level over 130mg/dl, 753 women underwent 100gm OGTTs at 28-32 weeks of gestation. According to the NDDG criteria, 113 cases with GIGT (single positive level of OGTT ; group 2), 125 cases with GDM (group 3), and 515 cases with control (group 1) were identified among the 753 cases. Retrospective review of outcome of these patients was performed. ANOVA and chi-square test were used to determine the statistical significance. Results : The incidence of GIGT and GDM was 2.7%, 3.0%. The prepregnant body mass index (21.4±3.0kg/m2, 21.3±2.8kg/m2, 23.2±4.1kg/m2), overweight of BMI over 26 (7.4%, 4.4%, 14.4%) and obesity of BMI over 30 (1.2%, 0.0%, 8.0%) was significantly higher in group 3 than group 1 (p<0.05). We defined poor maternal outcome as those suffering from any one of birth canal injury, hydramnios or oligohydramnios, preeclampsia, cesarean delivery due to cephalopelvic disproportion, dystocia, fetal distress. Group 3 showed most highest poor maternal outcome (22.3%, 28.3%, 39.2%, p<0.05). And we defined poor neonatal outcome as those suffering from any one of hyperbilirubinemia, hypoglycemia, congenital anomaly, admission to neonatal intensive care unit due to respiratory distress syndrome. Group 2 and group 3 showed poor neonatal outcome than group 1 (6.2%, 13.3%, 21.6%, p<0.05). Conclusion : Pregnancies complicated with GDM showed poor maternal and neonatal outcome, and GIGT experienced no adverse maternal outcomes but showed poor neonatal outcomes compared to normal pregnancy, and showed less correlation with obesity than GDM. Further study of pathophysiology and proper management of GIGT will be mandatory.

인간자궁내막의 탈락막화 (Decudualization)에 있어서 TGF-$\beta$ (Transforming Growth Factor-$\beta$)의 역할

박동욱,최동순,김미란,황경주,조미영,안성희,민철기,유희석,Park, Dong-Wook,Choi, Dong-Soon,Kim, Mi-Ran,Hwang, Kyung-Joo,Jo, Mi-Yeong,Ahn, Seong-Hee,Min, Churl-K.,Ryu, Hee-Sug 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.1

Objectives: To investigate the role of TGF (Transforming growth factor-$\beta$) involved in the paracrinic communication during decidualization between UEC (uterine epithelial cells) and USC (uterine stromal cells), we have employed a co-culture system composed of human endometrial epithelial and stromal cells in defined hormonal conditions. Design: In the co-culture, endometrial epithelial cells cultured in the matrigel-coated cell culture insert are seeded on top of the endometrial stromal cells cultured within a collagen gel. The co-culture was maintained for 48 hours under the following hormonal conditions: progesterone dominant condition (100 nM P4 and 1 nM E2) or estrogen-dominant condition (100 nM E2 and 1 nM P4). 10 ng/ ml HGF and/or 10 ng/ml TGF-$\beta$1 are added. Methods: RT-PCR is utilized to detect mRNAs quantitatively. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical staining are utilized to detect proteins in the tissue. Results: Prolactin mRNA is expressed in the co-cultured stromal cells under the progesterone dominant condition. TGF-$\beta$1 and its receptors are expressed in both the co-cultured epithelial and stromal cells irrespective of the steroid present, which is in contrast with no or negligible expression of TGF-$\beta$1 or its receptor in cells separately cultured. Both estrogen and progesterone significantly elevate the concentration of hepatocyte growth factor (HGF) in the conditioned medium of the co-culture with the value of 4, 325 pg/ml in E2-dominant and 2, 000 pg/ml in P4-dominant condition compare to 150 pg/ml in no hormone. In separately cultured stromal cells, administration of HGF induces the expression of TGF receptor 1 in both hormonal conditions, but induction of TGF receptor 2 is only manifest in the P4-dominant condition. Administration of TGF-$\beta$ and HGF directly induce the decidualization marker prolactin mRNA in separately cultured stromal cells. Conclusion: It is likely that steroid hormones induces prolactin mRNA indirectly by promoting the cell to cell communication between the stromal and the epithelial cells. TGF-$\beta$ and HGF are two possible paracrine mediators in the human endometrial decidualization.

증례 : 소화기 ; 담낭염 환자에 동반된 간문부에서 기시하는 이중담관 기형 1예

공은정 ( Eun Jeong Gong ),장은경 ( Eun Kyung Jang ),윤용훈 ( Yong Hoon Yoon ),안성희 ( Seong Hee Ahn ),현일식 ( Yil Sik Hyun ),황대욱 ( Dae Wook Hwang ),이성구 ( Sung Koo Lee ) 대한내과학회 2012 대한내과학회지 Vol.82 No.4

Anatomic variation in the hepatobiliary tract is relatively common, although bile duct duplication is rare. Detection of biliary tract variation is important because it is closely related to complications such as cholangitis, cholecystitis, pancreatitis, stone formation, and gastrointestinal tract malignancy. Therefore, preoperative imaging and a high index of suspicion are required to make an accurate diagnosis of a biliary tract anomaly. A 44-year-old woman presented with a 2-year history of epigastric pain after eating a meal and gallbladder sludge with chronic cholecystitis. The presumed diagnosis was gallbladder duplication. However, during surgery it was observed that the tubular structure was parallel with the fissure for the ligamentum venosum and ended at the lesser curvature of the stomach. The pathologic diagnosis was bile duct duplication. We report a case of a unique variant of bile duct duplication arising from the hepatic hilum. (Korean J Med 2012;82:465-469)

산전에 자연 관해된 태아 심방조동 1예

안성희,김행수,황경주,양정인,손병철,오준환,양성천,오기석 대한산부인과초음파학회 2001 대한산부인과초음파학회지 Vol.3 No.1