감정노동의 강도가 서비스 품질에 영향을 미치는가?: 보상유형의 효과

심재철(Sim, Jae Chul),김정포(Kim, Jung-Po),최민철(Choi, Min-Cheol) 한국비즈니스학회 2018 비즈니스융복합연구 Vol.3 No.2

Udenafil (Zydena)이 생쥐의 수정과 초기 배아 발달에 미치는 영향

천근수 ( Keun Soo Cheon ),심재철 ( Jae Chul Sim ),양회생 ( Hoe Saeng Yang ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.9

Objective: To determine the effect of udenafil, a cyclic monophosphate-specific type 5 phosphodiesterase inhibitor, on fertilization and early embryo cleavage of mice, Methods: This mammal study included randomly assigned male and female mice, The females were sacrificed after mating to evaluate their oocytes and embryos in four different time intervals following the treatment. Female mice were injected intraperitoneally with 5 IU of gonadotropin and human chorionic gonadotropin to stimulate follicular growth and induce ovulation. They were separately caged with males that had been gavaged with udenafil (0.06 mg/0.05mL) and allowed to mate. After 24, 48, 72, and 96 hours of time intervals, females were killed, their oviducts were dissected out, and retrieved embryos were assessed for blastomere number and quality. Fertilization rates and numbers of embryos were evaluated after treatment. Results: Fertilization rates were reduced in females that were mated with udenafil gavaged males. Over days 2-4, the numbers of embryo that of the treated group were significantly fewer than in the control group. There was also a tendency of impaired cleavage rates with those embryos. Conclusion: The impairments of fertility caused by udenafil have important implications for infertility centers and couples who are using this drug precoitally while attempting to conceive.

ATM 저속 가입자 정합장치에서 점-대-다중점 연결 셀 전송장치 구현

박상택(Sang-Taick Park),심재철(Jae-chul Sim),김영섭(Young-sup Kim),권율(Yool Kwon) 한국통신학회 1998 한국통신학회 학술대회 및 강연회 Vol.1998 No.12

임신 주기에 따른 산소라디칼 생성계 및 해독계 활성도의 변화

김종호(Jong Ho Kim),심재철(Jae Chul Sim),배철성(Cheol Seong Bae),윤혜원(Hae Won Yoon),이영기(Young Gee Lee),박윤기(Yoon Ki Park),이두진(Doo Jin Lee),이승호(Sung Ho Lee) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.2

Objective; This study was conducted to evaluate the effect of pregnancy on the activities of oxygen hee radical generating and scavenging system in the female rats. Material & Method; Rats weighing 200-220 gm were grouped to non-pregnant, 2nd trimester and 3rd trimester of pregnancy. The experiment was carried out following overnight fasting. Animals were anesthetized by administration of pentothal sodium, and blood was drawn via abdominal aorta. After exsanguination, the liver, kidney, heart, lung, with or without placenta tissues were excised immediately. The excised tissue was frozen in liquid nitrogen rapidly, and stored in liquid nitrogen for analysis. Results; The gain in body weight was higher in pregnant rats than in normal rats. Lipid peroxidation was not significantly different among all groups in the liver, kidney, heart, lung, and placenta tissue. Xanthine oxidase activity of the kidney in the 3rd trimester of pregnancy was lower than that of non-pregnant rats. Superoxide dismutase activity of the liver was significantly decreased in the 2nd and 3rd trimester of pegnancy compared with that of non-pregnant rats, and that of lung was also decreased than that of non-pregnant rats. Catalase activity of the kidney was decreased in the 2nd and 3rd trimester of pregnancy compared with that of non-pregnant rats. Glutathione content of the liver was markedly decreased in the 2nd and 3rd trimester of pregnancy compared with that of non-pregnant rats. Conclusion; In conclusion, these results suggest that oxygen free radical will not increase in the liver, kidney, heart, lung, and placenta during normal pregnancy, but in the cases of overproduction of oxygen free radical, the liver, kidney, and lung will have me chance of tissue damage because of decreased activity of some anti-oxidant enzymes and/or decreased amount of anti-oxidant materials.

과배란 미성숙 흰쥐에서 prolactin이 난소 내 tissue type plasminogen activator와 prostaglandin E2에 미치는 영향

김경원 ( Kyoung Won Kim ),심재철 ( Jae Chul Sim ),양회생 ( Hoe Saeng Yang ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.2

Objective: This study is to analyze the direct effects of hyperprolactinemia, cause of anovulation and infertility, on ovarian function. Methods: The prepubertal female Sprague-Dawley (SD) rats were obtained and ovulation was induced with PMSG and hCG s.c.. The rats were divided into four groups, which received the following treatments IP : saline 0.2 ml, 150 ug PRL, 300 ug PRL, 300 ug PRL plus 300 ug naloxone. The animals were killed and the oviducts were evaluated for the presence of ova. The ovary were then removed and evaluated under light microscopy. For changes of follicular t-PA and PGE2 concentration after PRL, immature female SD rats were stimulated as described above. At four hours after the hCG injection the rats were killed and the ovaried were removed. Each isolated ovaries were incubated in culture plate containing incubation medium or 300 ng PRL to be tested. And PRL plus gonadotropin in incubation medium was tested because of change of PGE2 concentration. After incubation period, t-PA and PGE2 were measured by EIA. Differences between groups were assessed by two-way ANOVA of variance followed Mann-Whitney U test or Kruskal-Wallis test for multiple comparisons. p<0.05 was considered statistically significant. Results: As result, prolactin transiently suppresses ovulation, especially with its increased concentration not by altering the ovarian morphology. But ovulation inhibition was reversed by naloxone injection. The level of t-PA in control and prolactin-treated group increased steadily in response to human chorionic gonadotropin administration, yet lower in prolactin-treated group. But PGE2 concentration was increased in gonadotropin mixed groups but not affected in prolactin-treated group despite a significant blockade of ovulation. Conclusion: Thus, further studies on the effect of high level prolactin on ovulatory function would significantly contribute toward the patient with hyperprolactinemia for managing infertility and maintaining appropriate female reproductive function.

산전 태아 체중 평가에 도플러 초음파의 이용

김종호(Jong Ho Kim),최석철(Suck Chul Choi),양회생(Hoe Saeng Yang),심재철(Jae Chul Sim),배철성(Cheol Seong Bae),윤혜원(Hae Won Yoon),강민아(Min A Kang) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.3

Objective: The objective of this study was to determine the relationship between the fetal doppler flow velocimetry and birth weight in low risk pregnancy population. Methods: From December 1995 to May 1996, We prospectively performed doppler study in 254 uncomplicated, term pregnant women, who visited Pohang Hospital, Dongguk University. Using pulsed color doppler, we measured umbilical artery RI, middle cerebral artery RI and middle cerebral-umbilical artery RI ratio within one week before delivery. Results: The result was that low birth weight group (below 2500gm) had very significant lationship with umbilical artery RI(P<0.01), middle cerebral artery RI(P<0.05) and middle cerebral-umbilical artery RI ratio(P<0.05), but there was no significant relationship in these blood flow indices between normal birth weight group (2501gm- 3999gm) and macrosomia group (above 4000gm). Conclusions: We concluded that application of doppler ultrasonopaphy in the assessment of fetal weight is somewhat helpful for identification of low birth weight, not for macrosomia.

장환형 (Large-circular) 안티센스를 이용한 난소암세포 성장 관련 유전자의 발굴

도경오 ( Kyung Oh Doh ),천근수 ( Geun Soo Chun ),심재철 ( Jae Chul Sim ),양회생 ( Hoe Saeng Yang ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.7

Objective: The aim of this study is to find out the genes which are related to ovarian cancer cell growth using large circular antisense library. Methods: Clones for antisense library were uni-directionally sub-cloned into pBS SK (-) vector. LC-antisense molecules were then purified from the culture supernatants of the bacterial competent cells superinfected with M13K07 helper bacteriophages. The LC-antisense library to 240 unigene clone was constructed and utilized in the identification of genes functionally involved in the growth of ovarian cancer cells. Results: The 17 numbers out of the 240 numbers of the antisense library exerted a marked inhibitory effect on the growth of SK-OV 3. Conclusion: The putative functional categorization of each gene was then conducted via public databases. These candidates may be used as target genes for drug development or adjuvant of conventional chemotherapeutic drugs.

미산부의 과숙임신에서 유도분만 시 제왕절개분만율이 증가와 관련된 위험인자

지경석 ( Kyung Suk Chi ),김준수 ( Jun Su Kim ),장은정 ( Eun Jeong Jang ),심재철 ( Jae Chul Sim ),양회생 ( Hoi Saeng Yang ),이형종 ( Hyung Jong Lee ),하준영 ( Jun Young Ha ),황지영 ( Jee Young Hwang ),김도균 ( Do Gyun Kim ) 대한주산의학회 2006 Perinatology Vol.17 No.1

목적 : 본 연구는 미산부에서 과숙임신으로 인한 선택적 유도분만 시 분만 방법 결과와 관련된 인자들을 분석하고자 이 연구를 시행하였다. 방법 : 1997년 3월부터 2005년 3월까지 임신 41 완전주수 이후에 선택적 유도분만을 위해 입원한 미산부 331명을 대상으로 하였다. 관련된 인자들로 연령, 체질량지수, 임신주수, Bishop 점수, 출생체중, 태아 두위, 태아 성별을 조사하였다. 결과 : 산모의 평균연령은 제왕절개분만군에서 27.59±2.57세이고, 질식분만군에서 26.99±2.61세였으며, 임신주수는 제왕절개분만군에서 평균 290±3일(41+3주), 질식분만군에서 289±2일(41+2주)이었다. 임신 전 평균 BMI는 제왕절개분만군에서 20.42±2.36 m2/kg, 임신 후 26.70±2.82 m2/kg로 증가하였으며, 질식분만군은 19.92±2.05 m2/kg에서 25.75±2.67 m2/kg로 증가하였다. 분만 후 태아 몸무게는 제왕절개분만군에서 3460.31±358.22 g이였으며, 질식분만군에서 3363.95±361.22 g이였다. 태아 두위는 제왕절개군에서 34.59±1.18 cm이고, 질식분만군에서 34.03±1.33 cm였다. 제왕절개군 127명 중 38명(29.9%)의 분만된 신생아에서 합병증(들)으로 출생 후 바로 입원치료 했으며, 질식분만군 204명 중 24명(11.8%)에서 나타났다. 결론 : 본 연구는 산모의 체중이 증가할수록, 태아의 몸무게 및 두위가 클수록 선택적 유도분만 후 제왕절개분만의 빈도가 증가함을 알 수 있으며, 이에 따른 태아의 유병율이 증가할 수 있다는 것을 증명했다. 그러므로 선택적 유도분만 전 산과의사는 적절한 방법과 충분한 경험을 토대로 제왕절개분만과 관련된 위험인자를 평가해야 할 것이다. Objective: The purpose of this study was to evaluate the risk of cesarean section in nulliparous women who undergone induction at postterm pregnancy. Methods : The retrospective study was conducted from March, 1997 to March, 2005 by reviewing 331 nulliparous patients more than 41 weeks` gestation delivered after induction at our Hospital. The evaluated variables to assess the risk of cesarean section were maternal age, body mass index (BMI), gestational age (GA), Bishop scores (BS), fetal body weight (FBW), fetal head circumference (FHC) and fetal sex. t-test and x2-test were used to compare these categorical variables. Results: The study included 331 nulliparous singleton pregnant women undergoing elective induction for postterm pregnancy: 127 (38.4%) delivered babies by cesarean section due to induction failure, progression failure and fetal distress, whereas 204 (61.6%) delivered vaginally. The mean maternal ages were 27.59±2.57 in cesarean delivery group and 26.99±2.61 in vaginal delivery group. The average values of BMI at postterm in cesarean and vaginal delivery groups were 26.70±2.82 and 25.75±2.67 kg/m2. GA was 41.36±0.27 weeks in cesarean delivery group, whereas 41.20±0.19 weeks in vaginal delivery group. The average FBW and FHC were 3460.31±358.22 g and 34.59±1.18 cm in cesarean delivery group, compared to 3363.95±361.22 g and 34.03±1.34 cm in vaginal delivery group. Conclusion : The BMI, FBW and FHC have linked to the risk of cesarean delivery in nulliparous women who underwent elective induction. Thus, these information would provide the useful tools to assess the risk of cesarean section in postterm nulliparous patients for planning an induction.

성숙 기형종에서 유래한 난소의 편평상피세포암 2 예

박영복(Young Bok Park),김승만(Sung Man Kim),조재훈(Jae Hun Jo),이동기(Dong Ki Lee),김경원(Kyoung Won Kim),김도균(Do Gyun Kim),김종호(Jong Ho Kim),양회생(Hoe Saeng Yang),윤혜원(Hae Won Yoon),심재철(Jae Chul Sim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.11

Between 1 and 2% of benign cystic teratomas undergo malignant transformation. This occurs most frequently in postmenopausal women, who account for only 10% of benign cystic teratoma. A wide variety of neoplasms arise in the mature tissues of otherwise benign teratomas. Of the malignancies arising in teratomas, squamous carcinoma is the most common (83%) with sarcomas accounting for 7%, adenocarcinoma and carcinoid tumors make up most of the others. The presentation in StageⅠ disease does not differ from that of benign cystic teratoma, except that ascites is occasionally present. In the more advanced neoplasms, the symptoms are those of epithelial ovarian cancer of the same stage. Differential diagnosis between squamous cell carcinoma and benign mature teratoma is difficult but seems to be related to age, size, serum tumor marker (SCC, CA125, CEA). Prognosis of squamous cell carcinoma in mature cystic teratoma was reported much poorer than other epithelial ovarian cancers. In the early stages, treatment is possible through surgical intervention alone, but when advanced, 5 year survival rate shows less than 15 percent. We experienced two cases of squamous cell carcinoma of ovary arising in mature teratoma and present with a brief review of literature.

Fentanyl과 Midazolam이 생쥐 체외수정과 배아발달에 미치는 영향

정상윤(Sang Yoon Jung),이은주(Eun Joo Lee),양회생(Hoe Saeng Yang),심재철(Jae Chul Sim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6

Ojective: To assess the effect of fentanyl and midazolam on in vitro fertilization rate and early embryo development in a mouse IVF model.Methods: Mouse oocytes were exposed in vitro to fentanyl at a concentration of 0(control), 50, 250, 500, 1000, 5000 pg/ml, and midazolam, 0(control), 2.5, 12.5, 25, 50, 250 ng/ml for 30 minutes, washed and inseminated. Thereafter fertilization was assessed. And subsequent in vitro development to the blastocyst stage was monitored daily.Results: Where fertilization occurred, subsequent embryo cleavage and development up to the blastocyst stage was affected significantly by the presence of fentanyl and midazolam solution in the medium(i.e., 14% to 31%, 10% to 35%), in comparison with control group( 60%, 62%).Conclusion: It can be concluded from these experiments that even a brief exposure of cumulus enclosed oocytes to a low concentration of fentanyl, midazolam is deleterious to subsequent cleavage.