산양유의 체세포수 등급 설정에 관한 연구

신지혜,정석근,한기성,장애라,채현석,유영모,안종남,우광태,최석호,이완규,함준상,Shin, Ji-Hye,Jeong, Seok-Geun,Han, Gi-Sung,Jang, Ae-Ra,Chae, Hyun-Seok,Yoo, Young-Mo,Ahn, Chong-Nam,Woo, Kwang-Tae,Choi, Seok-Ho,Lee, Wan-Kyu,Ham, Jun-Sang 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.2

The aim of this work was to establish the standard for goat milk somatic cell counts. The data were obtained from MGEN, which were collected from Dec. 2006 to Nov. 2007. A total of three hundred and forty somatic cell counts from 12 goat milk farms were analyzed. A goat milk grading system by somatic cell count is proposed; less than 1,000,000/mL, 1,000,000-1,500,000/mL, 1,500,000-2,000,000/mL, 2,000,000/mL-2,500,000/mL, and over 2,500,000/mL. Under the grading system, the ratio of first grade goat milk would be 26.2%, and that of the fifth grade would be 11.8%. The first grade ratio is low and the fifth grade ratio is high compared to the cow milk grading system. It is expected that somatic cell counts of domestic goat milk will be improved by the proposed grading system.

조선후기 영악전(靈幄殿) 기능수용에 따른 정자각 평면변화 고찰

신지혜,Shin, Ji-Hye 한국건축역사학회 2009 건축역사연구 Vol.18 No.4

The "Jeongjagak(丁字閣, T shaped building)" was important building from Goryeo Dynasty to Joseon Dynasty. For long period, the scale and form of the building had been changing bit by bit. The change of building results from the function. As the Jeongjagak accepted the functions of the Yeongakjeon(靈幄殿), there appeared changes of plane. The main function of Yeongakjeon was suppling space for the dead king's coffin. The Yeongakjeon was not built in the first year of King Sukjong(1674). At that time, the Jeongjagak was responsible for the function of the Yeongakjeon as an alternative. Starting from this, the Jeongjagak was used as space for the dead king's coffin. Because the coffin should place on from south to north, it demanded long inner space in south-north direction. Therefore the effort to make enough length in south-north direction was begun from the first year of King Sukjong(1674). In order to solve the problem, the Toigu(退構) was made from the reign of King Sukjong to the 28th year King Youngjo(1852). The Toigu was temporary inner space which was made in one compartment at the Baewichung(拜位廳, the part of the Jeongjagak). But the length of the Jeongjagak's south-north direction was reduced to 61.8m(20尺6寸) at the 33th year of King Youngjo(1857) when the "Gukjosangryebopyon(國朝喪禮補編)" was completed. Also it extended to 84m(28尺) during the reign of King Jeongjo(1774~1800). Following these process of extension and reduction, the length was standardized as 72m(24尺) at the reign of King Sunjo(1800~1834). These facts explains that the main cause of plane change at the Jeongjagak was acceptance of functions that was used as space for the dead king's coffin. Also, the important points of change at the Jeongjagak were the first year of King Sukjong, the 33th year of King Youngjo and the first year of King Sunjo. When it was the first year of King Sukjong and the 33th year of King Youngjo, there were two national funerals. Because of concern about the increasing labor and tax of the nation, the scale of the Jeongjagak was changed to decreasing size. Due to the improvement of drawings and annotation on a Eugwe(儀軌) at the first year of King Sunjo, the size of Jeongjagak was standardized.

참나무 톱밥을 이용한 표고 지면재배 과정의 주요 미생물 군집 분석

신지혜,윤서연,남지현,구창덕,이동훈,Shin, Ji-Hye,Yun, Seo-Yeon,Nam, Ji-Hyun,Koo, Chang-Duck,Lee, Dong-Hun 한국미생물학회 2015 미생물학회지 Vol.51 No.3

톱밥발효를 이용한 표고버섯의 지면재배방법은 적은 노동력을 소요하고 표고의 생산력을 증대시킬 수 있는 방법이다. 이 방법은 미생물에 의한 참나무 톱밥의 발효과정과 표고균사를 접종하고 생장시키는 과정의 두 단계로 나누어진다. 본 연구에서는 참나무 톱밥을 이용한 표고 지면재배과정의 각 단계에서 우점하는 주요 미생물을 확인하고 효율적인 표고버섯 재배를 위한 정보를 제공하고자 하였다. 발효과정이 진행되며 온도가 상승함에 따라 톱밥의 고온 세균의 비율은 10%에서 80%까지 증가하여 중온성 세균에서 고온성 세균으로 군집의 천이가 확인되었다. 16S rRNA 유전자를 이용한 T-RFLP 방법과 염기서열 분석 방법으로 참나무 톱밥 지면재배과정의 단계별 미생물 군집의 변화를 확인했다. 발효 전 참나무 톱밥시료에서는 중온성 세균인 Enterobacteriaceae 과의 세균이 우점(100%)하였고, 발효가 진행되며 Amycolatopsis (49.0%), Saccharopolyspora (26.5%) 등의 고온성 방선균으로 미생물 군집의 천이가 발생되었다. 특히, Amycolatopsis 속이 최고온도가 유지되는 발효과정 중에도 항상 우점한 결과를 고려하면 발효를 주도하는 미생물이라고 생각된다. 균사생장시기에서는 저온성 세균인 Leuconostoc이 우점(75.0%)하였다. 표고 균사의 활발한 성장을 위해서는 참나무 톱밥의 발효과정이 매우 중요하기 때문에 발효과정에서 우점한 고온성 방선균인 Amycolatopsis 속에 관한 다양한 연구가 필요할 것으로 생각된다. Ground bed cultivation method for shiitake mushroom (Phogo; Lentinula edodes) has been advanced to use the oak-sawdust composted by microorganisms. This method has some advantages in saving the labors and improving productivity. The aim of this study is to analyze microbial community in oak sawdust and to provide the information for efficient cultivation of shiitake mushroom. In process of composting, the proportions of thermophiles increased from 10% to 80%. This results show the microbial community succession from mesophilic bacteria to thermophilic bacteria. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of dominant bacteria coupled with shifts in each stages. The family of Enterobacteriaceae as a mesophilic bacteria were dominant (100%) in oak sawdust sample, but Amycolatopsis (49.0%) and Saccharopolyspora (26.5%) as a thermophilic Actinomycetes were dominant in the last day of composting. In hyphal growth stage, Leuconostoc (psychrophilic bacteria) was dominant (75.0%). The composting process of sawdust is very important for growth of mushroom mycelium. Therefore, there is need for the further study for the Amycolatopsis as thermophilic Actinomycetes.

구뇌탕(救腦湯)을 비롯한 한의치료로 호전된 긴장형 두통 환자 증례 보고 4례

신지혜,조윤영,백태현,박해모,선승호,Shin, Ji-hye,Cho, Yoon-young,Baik, Tai-hyeun,Park, Hae-mo,Sun, Seung-ho 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.2

Objectives: The purpose of this case report is to describe the effect of Korean medicine therapy on patients with tension-type headaches. Methods: This study consisted of four patients with tension-type headaches. The patients were treated with Korean medicine therapies, such as herbal medicine (Gunoe-tang), acupuncture, and cupping, in addition to physical therapy. None of the patients took any analgesics during the admission period. To evaluate the effects of the treatment, the Numeric Rating Scale (NRS) for headaches was used. Results: After the treatment, the patients' complaints decreased, as shown by the results of the NRS. Conclusion: Treatment with Korean medicine may ameliorate tension-type headaches.

조선후기 산릉의 여성공간, 나인가가(內人假家)의 변화에 관한 연구

신지혜,Shin, Ji-Hye 한국건축역사학회 2012 건축역사연구 Vol.21 No.5

On behalf of the royal women, SangGung(尙宮:The head of female servants in the palace) and NaIns(內人: Female servants caring for royal families in the palace) were dispatched in order to attend a funeral at the royal tomb. The NaInGaGa(內人假家) is the temporary building for SangGung and NaIns in the royal tomb. It is comprised of lodgings for them and also workrooms and warehouses to prepare ritual offering for the dead King or Queen. In the early Joseon dynasty, the NaInGaGa was utilized until a funeral at the royal tomb. Since 1674, NaInGaGa for the 3 years-period lamentation was started constructing separately. At these processes, the plan and placement of NaInGaGa was changed. This study based on the SanReungDoGam-EuiGwae (山陵都監-儀軌: The report on constructing royal tomb). The SanReungDoGam-EuiGwae written since 1800 have illustration about NaInGaGa. The illustration and explanation about NaInGaGa become a important clue that make suppose detailed space of NaInGaGa.

조선조 숙종대 혼전조성과 그 특징에 관한 연구 - 창경궁 문정전을 중심으로 -

신지혜,Shin, Ji-Hye 한국건축역사학회 2010 건축역사연구 Vol.19 No.3

This study intends to look into management and architectural space composition of Honjeon during the reign of Sukjong. Also it purposes to inspect how to affect management of the Royal Palace. The study is based on each Binjeon Honjeon Dogam Eugwe between late 17C and 18C. And the data on management of the Royal Palace is based on Joseonwangjosillok and Seungjeongwon Ilgi. Because Sukjong used ChangDeokgung and GyeongDeokgung alternately, Honjeon located at Munjeongjeon of ChangGyeonggung during Sukjong stayed at ChangDeokgung and located at Gyesangdang of GyeongDeokgung during Sukjong stayed at GyeongDeokgung. At that time, often movement of the King modified procedure of moving Honjeon. Between the first year of Hyenjong and the 9th year of Sukjong, architectural space of Honjeon was settled. In 1659, territory of Jeongjeon was settled. Also between 1674 and 1684, Goklimcheong was built. Construction of Goklimcheong is noticeable character. As Munjeongjeon was being used as Honjeon continually, it was recognized as Honjeon. Due to its long utilization as Honjeon, several gonvernment offices were transferred. Also it influenced utilization of Myeongjeongjeon.

음식물 쓰레기 퇴비화 과정에 따른 세균군집 구조의 변화

신지혜,이진우,남지현,박세용,이동훈,Shin, Ji-Hye,Lee, Jin-Woo,Nam, Ji-Hyun,Park, Se-Yong,Lee, Dong-Hun 한국미생물학회 2009 미생물학회지 Vol.45 No.2

퇴비화 과정은 유기성 폐기물을 비료와 같은 유용한 자원으로 전환하는 생물학적 과정이다. 본 연구에서는 음식 물 쓰레기를 2달 동안 퇴비화시켜 세균군집의 변화를 조사하였다. 온도의 변화를 기준으로 하여 퇴비화 과정은 1단계($2\sim55^{\circ}C$), 2단계($55\sim97^{\circ}C$), 3단계($50\sim89^{\circ}C$)로 나뉘었다. 각 단계별 총세균수는 1단계 $1.66\times10^{11}$ cell/g, 2단계 $0.29\times10^{11}$ cell/g, 3단계 $0.28\times10^{11}$ cell/g으로 관찰되었다. 또한 총세균수에 대한 고온미생물의 비율은 초기에 33% 였으나 2단계 시료에서 최대비율인 89%로 증가하였다. 16S rRNA 유전자를 대상으로 T-RFLP 방법과 염기서열 분석방법을 이용하여 세균군집의 구조가 퇴비화 과정에 따라 변화됨을 확인할 수 있었다. 초고온인 2단계의 세균군집의 발달은 스타터 접종의 영향을 받았으며, Bacillus 및 Pseudomonas와 유연관계가 가까운 세균군집이 퇴비화 과정을 이끄는 주요 미생물임을 확인하였다. Composting is a biological process converting solid organic waste into valuable materials such as fertilizer. The change of bacterial populations in a composting reactor of food waste was investigated for 2 months. Based on shifts in temperature profile, the composting process could be divided into the first phase ($2^{\circ}C\sim55^{\circ}C$), the second phase ($55^{\circ}C\sim97^{\circ}C$), and the third phase ($50^{\circ}C\sim89^{\circ}C$). The number of total bacteria was $1.66\times10^{11}$ cell/g, $0.29\times10^{11}$ cell/g, and $0.28\times10^{11}$ cell/g in the first, second, and third stages, respectively. The proportions of thermophiles increased from 33% to 89% in the second stage. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of bacterial community structure was coupled with shifts in composting stages. The structure of bacterial community in the ultra-thermophilic second stage reflected that of seeding starter. The major decomposers driving the ultra-thermophilic composting were identified as phylotypes related to Bacillus and Pseudomonas.

감마선 조사에 의한 분유 단백질의 항원성 저감화

신지혜,정석근,한기성,장애라,채현석,유영모,안종남,이주운,조철훈,이완규,함준상,Shin, Ji-Hye,Jeong, Seok-Geun,Han, Gi-Sung,Jang, Ae-Ra,Chae, Hyun-Seok,Yoo, Young-Mo,Ahn, Chong-Nam,Lee, Ju-Woon,Jo, Cheo-Run,Lee, Wan-Kyu,Ham, Jun-Sang 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.3

The aim of this work was to examine the reduction in antigenicity of milk proteins in powdered milk by gamma irradiation which is increasingly used for food safety. Skim milk powder samples were exposed to irradiation doses of 1, 5, and 10 kGy. A greater reduction of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein was found than ${\alpha}_{S0}$-casein and ${\beta}_{A2}$-casein by capillary electrophoresis. Competitive indirect ELISA and passive cutaneous anaphylaxis tests using guinea pigs showed a reduction in antigenicity of powdered milk by 10kGy gamma irradiation. These results indicated that gamma irradiation reduce allergenicity of milk proteins by structural changes of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein, and can be useful for dairy products.

17~18세기 조선왕릉의 봉릉 구조개선에 따른 사방석(四方石)의 등장과 소멸

신지혜,Shin, Ji-Hye 한국건축역사학회 2021 건축역사연구 Vol.30 No.4

This is a study on the structural changes of the tumulus and causes setting up a Sabangseok at the front of the tumulus at the royal tomb in the JoSeon dynasty, during the 17th~18th centuries. The Sabangseok was first used in the tumulus of Mongnueng(穆陵) in 1630. It is a multipurpose stone for boundary, foundation of the tumulus and blocking the sliding down of the tumulus. It was set up, when constructing a Yeongneung(寧陵) in 1673. But the vast spill of tumulus soil made it improve structure of the Sabangseok. Consequently, when the Yeongneung was re-constructed, the structure of the tumulus was fundamentally improved. The soil layer on the lime of the subterranean chamber became eliminated. Also the lime of the tumulus and the lime of the subterranean chamber became a united structure. The Sabangseok was still used until 1757 on account of precedents, although it would become unnecessary after structural improvement of the tumulus in 1673. In 1757, Yeongjo(The 21th monarch of the Joseon Dynasty) commanded repeal on the use of the Sabangseok, when constructing the Hongneung(tomb of Queen Jeongseong). The decision and discussions about abolition on the use of the Sabangseok was recorded in Gukjosangryebopyeon.

뇌교경색 이후 발생한 편마비, 현훈 및 딸꾹질 치험 1례

신지혜,남해인,조윤영,선승호,백태현,김선혁,Shin, Ji-hye,Nam, Hae-in,Cho, Yoon-young,Sun, Seung-ho,Baek, Tae-hyun,Kim, Seon-hyeok 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.2

Objective: : This clinical study reports on the effect of Sunghyangjungki-san-gami and Banhabaeckchulchunma-tang-gami on hemiplegia, dizziness, and hiccupping caused by pontine infarction.Methods: This study was performed on one patient with hemiplegia, dizziness, and hiccupping caused by pontine infarction. The patient was treated by Sunghyangjungki-san-gami and Banhabaeckchulchunma-tang-gami from 6 January to 2 March 2015. The National Institutes of Health stroke scale (NIHSS), motor grade, and numerical rating scale (NRS) were used to assess the effectiveness of this treatment.Results: After treatment, the patient’s discomfort decreased significantly, and NIHSS, motor grade, and NRS scores were all improved.Conclusion: This clinical study suggests that traditional Korean medicine may be effective in treating hemiplegia, dizziness, and hiccupping caused by pontine infarction.