http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
다채 (Brassica campestris var. narinosa) 유묘의 형질전환 및 일시발현의 정량적 분석
신동일,박희성,Shin Dong-Il,Park Hee-Sung 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4
Brassica campestris var. narinosa는 새싹채소로 애용되는 식물로서 간단한 용기에서 손쉽게 재배할 수 있다. 본 연구에서는 vacuum-infiltration을 통한 Agrobacterium-mediated transformation에 의해서 B. campestris var. narinosa 싹에서의 GUS일시발현을 성공적으로 확인하였다. 발아전의 종자가 발아를 이미 시작한 종자보다 그 형질전환 효율이 높게 나타났다. 한편, hydrogen peroxide를 2일 생장시킨 싹에 처리한 후 형질전환 하였을 때 GUS발현이 증가되는 것을 관찰하였다. 간염항원유전자의 형질전환과 ELISA에 의한 항원단백질의 발현량 분석 시 hydrogen peroxide 처리 싹이 비처리 형질전환 싹에서 보다 2배 이상의 발현량이 측정되었다. Tah tasai chinese cabbage (Brassica campestris var. narinosa), a vegetable plant popularly consumed as several-days-old seedlings in oriental countries, can be easily cultivated using a simple appliance. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration (agroinfiltration) resulted in a successful transient GUS gene expression in tah tasai chinese cabbage seedlings. Pre-germinated seeds were found to be more susceptible to Agrobacterium infection than one-day-old or two-days-old seedlings. We also demonstrated that hydrogen peroxide (HPO) treatment increased GUS expression especially for two-days-old seedlings. In ELISA using seedlings transformed with hepatitis B surface antigen (HBsAg) DNA by agroinfiltration, HBsAg protein synthesis increased more than two folds by HPO treatment to two-days-old seedlings in comparison to the mock-treated pre-germinated seeds.
신동일,박희성,Shin Dong-Il,Park Hee-Sung Korean Society of Life Science 2005 생명과학회지 Vol.15 No.6
배추 종자 및 유묘에 대하여 GUS발현 또는 hepatitis B surface antigen (HBsAg)발현 벡터를 지니는 Agrobacterium tumefaciens LBA4404 세포를 이용하여 진공침윤(agroinfiltration)에 의한 형질전환을 시도하였다. 특히 ELISA를 이용한 HBsAg발현의 정량적 분석에서 agroinfiltration방법은 형질전환효율이 매우 저조하게 나타났다. 그러나 차아염소산나트륨 용액을 발아 전 또는 발아 중인 배추종자에 처리한 후 agroinfiltration을 실시한 경우 형질전환 효율이 $2\~5$배 증가하였다. 따라서 차아염소산나트륨 등의 화학연마제에 의한 종자의 상처발생이 Agrobacterium의 감염을 용이하게 함으로써 배추유묘에서의 일시유전자발현을 증대시키는 것으로 제안되고있다. Chinese cabbage (Brassica campestris ssp. napus var. pekinensis Makino) seeds/seedlings were transformed via vacuum-infiltration with recombinant Agrobacterium tumefaciens LBA4404 cells. The agroinfiltration method was determined to be unsuccessful for Chinese cabbage transformation during the analysis of hepatitis B surface antigen expression by ELISA. However, treatment of sodium hypochlorite solution, prior to agroinfiltration, to pregerminated or germinating 1 day- or 2 days-old seeds was proven effectively to enhance transformation efficiency, suggesting that chemical wounding caused by sodium hypochlorite reaction might facilitate Agrobacterium infection and, therefore, transient gene expression in Chinese cabbage sprouts.
온실에서 자라는 낙엽송의 접목묘와 야외에서 자라는 실생묘로부터 (實生苗) 개화의 (開花) 유도
신동일 ( Dong Ill Shin ) 한국산림과학회 1996 한국산림과학회지 Vol.85 No.3
GA4/7 spray, injection and several cultural treatments were applied to the greenhouse-grown potted hybrid larch(Larix decidua × leptolepis) grafts and field-grown European larch seedlings to induce early flowering. A treatment consisting of repeat-ed GA4/7 sprays, alone, was the most effective flower induction treatment for greenhouse-grown, potted larch grafts. Root pruning as a adjunct treatment did not show synergistic effects. Injection for potted grafts with GA4/7 was not useful approach in this study and it resulted in increased mortality. In the field experiment with 10-Year-old larch seedlings, repeated GA4/7 sprays in combination with root pruning or with plastic mulching appears to be useful and practical means for inducing larch flowers:
신동일 ( Dong Il Shin ),김주연 ( Joo Yun Kim ) 21세기영어영문학회 2012 영어영문학21 Vol.25 No.2
The purpose of this study is to understand Korean test-takers` perceptions on TOEIC from critical language testing (Shohamy, 2001a). The interview data of this study were acquired from eleven college students who have learned TOEIC in everyday contexts. The results are found as follows: Firstly, test-takers saw TOEIC as an institutionalized test. They associated TOEIC with ``spec``, ``scale``, and ``gate keeper``. Secondly, TOEIC was perceived as a perpetuating tool of English status and successful career in Korea. Lastly, most participants took TOEIC-based English learning as building test-wiseness skills although a few expressed positive attitude toward TOEIC preparation. This study implies that TOEIC cannot be viewed as a neutral and positive measure of English proficiency, and that ``use-oriented testing`` needs to be considered in high-stake testing contexts, for the use of shared authority, collaboration and involvement of different stake holders. Implications for further research are also discussed.
대중문화에서 재현된 영어시험과 수험자 분석: 문화연구, 정체성, 기호학의 접점으로부터
신동일(Dongil Shin) 한국응용언어학회 2020 응용 언어학 Vol.36 No.2
This paper is intended to explore the meaningfulness of interdisciplinary approaches to cultural studies, identities, and semiotics in the field of language education and applied linguistics in Korea. The ‘cultural turn’, which notes representation issues in mass culture, identity construction, and semiotics, are reviewed and intertwined to lead to the question of how high-stakes test takers are described in the mass media. 10 printed advertisements in which test takers are modeled to prepare for NEAT (National English Ability Test) and TOEIC (Test of English for International Communication) were analyzed through syntagmatic and paradigmatic analysis, as well as through the Barthes’ signification analysis. Results obtained from semiotic analysis revealed that NEAT and TOEIC learners were often described in a stereotypical manner, positioned as taken-for-granted, competitive, emotional test-takers in conventional teach-to-the-test culture. The (distorted) representation of test takers discussed in this study, however, needs to be researched in different media settings, since the values of test prep appeared multiple, conflicting, and even entertaining in the present study, especially in the recent coverages. Meanings surrounding TOEIC learners, for example, could be interpreted as both forced and voluntary, competitive and friendly. It was found that the research tradition built on cultural studies, along with semiotic approaches, has great potential to critically understand a better meaning-making signification in the field of language education industry.
온라인 게임 서버를 위한 부하 분산 미들웨어의 구현에 대한 연구
신동일(Dongil Shin),신동규(Dongkyoo Shin),김민수(Minsoo Kim),장재홍(Jaehong Jang),윤현숙(Hyunsook Yoon),이정훈(Junghoon Lee),한창완(Changwan Han) 한국정보과학회 2000 한국정보과학회 학술발표논문집 Vol.27 No.2Ⅰ
오늘날 온라인 게임 TCP망을 이용하여 동시에 수백에서 수천명이 접속하여 게임을 즐길 수 있는 클라이언트/서버 모델의 표준이지만 서버에 접속하는 클라이언트의 수가 증가함에 따라 나타나는 많은 문제점을 가지고 있다. 본 논문에서는 기존 네트웍 게임 엔진의 구조적 단점을 보완한 3-tier 방식을 적용한 분산 네트웍 게임 서버를 위한 부하 분산기의 연구 및 개발에 대하여 서술한다. 이 시스템은 클라이언트/서버 모델의 2-tier방식에서 오는 클라이언트 수 증가에 따른 네트웍 부하 가중에 대한 처리 문제와, 분산된 2-tier방식을 적용한 서버에서 각 서버간의 통신, 데이터 공유에 대한 문제 등을 다루고 이에 대한 해결책으로 3-tier방식을 적용하여 클라이언트/서버사이에 각 서버의 사용자나 이동되는 데이터를 분산하고 모니터링하는 부하 분산기 시스템을 적용하여 위와 같은 문제점을 해결하였다.