주요 국산밀 품종과 내고온성 터키 유전자원을 이용한 내고온성 관련 SSR 마커 평가

손재한,김경훈,정영근,박종철,김경호,김양길,오영진,송태화,김보경,강천식 한국작물학회 2015 한국작물학회지 Vol.60 No.3

밀의 등숙기간, 종자 수, 종자무게와 관련된 SSR 마커14개를 이용하여 터키에서 분양받은 내고온성 유전자원23개와 국산밀 품종 7개, Chinese spring 1개 등 31개를분석한 결과, 전체 86개의 대립유전자(평균 6.14개)가 확인되었다. 평균 PIC 값은 0.64로 나타났다. 다형성 분석을통한 마커 데이터를 이용하여 계통 분석을 한 결과 크게세 그룹으로 형성되었다. 올밀이 가장 바깥 그룹으로 형성되었고 올그루, 고소, 조품 등이 터키자원과 다르게 단일그룹으로 형성되었다. 금강, 조경, 백중 등 세 품종은 터키자원들과 같은 그룹으로 나타났지만 밀접하게 연관되어있지는 않은 것으로 나타났다. 국내 품종 중 올, 올그루, 조품, 고소 등 4개와 금강, 조경, 백중 등 3개가 서로 다른그룹으로 형성되었다. 두 그룹의 차이는 파성 II와 III의 차이로 구별되었다. High temperature is one of major environmental stress. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR etc. was tried to select useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. We tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey, six Korean wheat cultivars and Chinese spring. The average of the number of alleles and PIC values in this study were 6.14 and 0.64, respectively. Two major clades and four sub clades were grouped by phylogenetic tree using UPGMA. Four Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance. Keywords : heat tolerance, MAS, SSR, wheat

밀 알레르기 연구 동향

손재한,정영근,박종철,김경호,김보경,강천식 한국육종학회 2017 한국육종학회지 Vol.49 No.2

Wheat is one of major crop and wheat flour is used to various end-use products such as bread, cookies and noodles due to its unique characteristic of it as elasticity and viscosity. Wheat consumption has been generally increasing in not only US and Europe but also Korea. Nevertheless, gluten proteins in wheat endosperm are cause of allergy by food ingestion. Hence, studies on the allergy have been conducting and have been attracting public attention. Herein, we report studies on research trend of the issue with research papers over the last decade for suggestion of future research direction. Since 2012, studies on allergy of gluten proteins have been dramatically increased based on the number of published papers related to the issue. As results of research activities by country, the Europe accounted for 60% corresponding to the number of papers. Next US (13%), Japan (9%), China (5%), and Korea (2%) came. In Korea, studies on allergy of gluten protein are less studied. In the field of studying of gluten allergy, there are two major technologies as preclinical technology and genome research accounted for 58% and 26%, respectively. In Korea, the study on glutenin proteins which is closely related to wheat allergy is actively performed. Therefore, it can be expected that research will be become more active.

성숙기의 고온처리에 따른 밀의 농업형질 특성 변화 분석

손재한,강천식,정영근,김양길,박종호,박태일,윤영미,송태화,김보경 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-

Species Relationships among Allium Species by ISSR Analysis

손재한,박경철,이성일,김종화,김남수 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.3

The genus Allium includes several important condiment and ornamental vegetable species. The botanical status of many more than 600 species in the genus Allium has not been clearly classified. We used inter simple sequence repeat (ISSR) technique to analyze the phylogenetic relationships among 24 individual accessions and species that represent cultivated Allium species and their ancestral species. Nucleotide sequence analysis was used to rule out homoplasy,the possibility that unrelated ISSR-bands migrated to the same positions during gel electrophoresis. To avoid spurious results, PCR reactions were conducted in duplicate on independent thermocyclers and only reproducible bands were used for analysis. We detected 153 polymorphic bands from 20 ISSR primers. The ISSR polymorphism profiles were used to group most of the Allium species and accessions according to previous classification. However, A. chinense,previously included in the Rhizirideum section, was distinct from other species in the phylogenetic dendrogram. A. wakegi,the Korean endemic ‘Jjokpa’ and hybrid between A. cepa and A. fistulosum, was grouped in the Cepa section. A. monanthum, another Korean endemic ‘Dalae,’ was grouped in the Rhizirideum section. Because phylogenetic analysis of these three Korean endemic species has not been reported previously, the current report may allow these species to be included in future phylogenetic studies.

분자표지인자를 이용한 밀 연구 현황과 국산 밀의 연구 방향

손재한,김경훈,신상현,김학신,김남수,현종내,강천식 한국육종학회 2013 한국육종학회지 Vol.45 No.3

식물연구에 있어 다양한 분자마커의 개발은 학문적 그리고 기술적으로 새로운 혁신을 가져왔다. DNA를 이용한 분자마커는 DNA 지문뿐만 아니라 식물의 분류학, 생리학 및 유전공학 등 다양한 분야에서 사용되고 있다. 밀은 옥수수, 벼와 함께 세계 중요 3대 작물 중 하나로서 17,000 Mb의 게놈을 가지고 있어 연구에 많은 어려움이 따르고 있다. 본 연구는 현재까지 보고된 분자마커를 이용한 밀 연구 분야에 관한 보고들을 분석하여 앞으로의 연구 방향을 제시하고자 하였다. 1990년부터 2012년까지 발표된 주요 논문 1,123편을 분석한 결과 유전육종 분야가 38.3%로 가장 높았으며, 분자표지인자와 생리학 분야가 각각 26.9%와 1.5%로 나타났다. 미국을 포함하여 중국, 호주 영국 등 4개국에서 발표한 논문이 전체의 50% 이상을 차지하고 있으며, 이들 국가소속기관 연구소에서 발표된 논문이 전체의 52%로서, 대학보다 활발히 진행되고 있는 것을 알 수 있었다. 특히 밀의 연구는 1992년부터 2007년까지 급격하게 증가하였는데 이것은 밀의 다양한 육종사업과 분자표지인자 기법의 발달이 영향을 미쳤기 때문이다. 그러나 국내 밀의 연구 논문은 88편으로 2008년 이후 대부분 보고되는 것으로 보아 밀의 연구가 매우 늦게 시작된 것을 알 수 있다. 현재까지 국내 밀의 연구는 유전육종 분야에 집중되어 있는 것을 알 수 있다. 국산 밀은 ‘금강’ 등 30 여 품종이 생산량 증가, 재해저항성 증진 등의 목적으로 육성되었다. 예를들어 백립계 제면용으로서 내한성과 붉은곰팡이 중도 저항성을 가지는 ‘한백’, ‘적중’, ‘알찬’, ‘수강’ 등의 품종이 개발되었다. 이와 같이 다양한 목적에 맞는 밀 품종이 육성되어 있기 때문에 이 후의 연구는 용도별 신품종 육성과 함께 국산 밀 품종을 보호하는 방향으로 나아가야할 것이다. 이를 위해선 국산 밀의 기본적인 식물적 특성과 유전적 특성이 확립되어야한다. 최근 국산 밀의 품질 및 품종 판별에 적합한 분자표지인자를 개발하기 위한 다양한 분석방법이 시도되고 있다. 다양한 분자표지인 자 기술 및 개발은 앞으로의 밀 육종사업에 유용한 도구로서 이용가치가 매우 높기 때문에 SSR, ISSR, STS 등의 분자표지인자를 개발하여 국내 밀품종을 보호할 뿐만 아니라, 밀의 수량성 및 고품질과 내재해성 등에 관련된 많은 연구를 통하여 밀의 품질과 수량성 향상에 이용해야 할 것이다. Academic and technical development of a variety of molecular markers in plant research has brought new innovations. DNA molecular markers have been used in a variety of fields, such as plant taxonomy, physiology and genetic engineering, as well as simple DNA fingerprints. Wheat is one of the three important crop with maize and rice. However, wheat have so enormous genome (17,000 Mb) that they have a lot of trouble to research follow. In this study, we analyzed that the reported on the research field of wheat have been reported until now, and was to provide direction for future research. The results of analysis of 1,123 episode of major papers published from 1990 to 2012, the field of genetics and breeding was highest 38.3%, molecular marker was 26.9% and physiology was 1.5%, respectively. In Korea, 35 wheat cultivars were developed such as ‘Kumkang”. Most of all the Korean wheat cultivars are developed for specific applications that is baking or noodles. Therefore, the development of specific molecular markers related to abiotic or biotic stress, yield and quality of wheat flour are able to use useful tools to protect wheat varieties, as well as the wheat breeding programe, the quantity and quality of wheat.

韓国語光陽市津上面方言のアクセント

손재현(孫在賢) 일본어문학회 2014 일본어문학 Vol.64 No.-

The objective of this paper is to describe the accent systems and their mechanisms of a dialect in the Gyeongsang province of Korea. I will discuss on the three-class type in this paper found in this areas, and report on data obtained during field work there. The three-class system proposed in this paper can be found in Gyeongsang-do, Jella-do, Chungcheng-do, and the following neighboring regions surveyed by the present writer: Sangju-si, Gumi-si, Gimcheon-si, Geochang-gun and Hamyang-gun, with the Sobeck mountain range at the center. I have previously discussed the accents of this area. In this paper, I will take Gwangyang dialect, and discuss phenomena, such as the accents of compound words, which have not yet considered.

밀 주요 농업 형질관련 마커 특성 분석

손재한,강천식,정영근,김양길,박종호,박태일,윤영미,송태화,김보경 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-

등숙기 고온이 국내 밀 품종의 밀가루 및 가공적성에 미치는 영향

손재한,강천식,윤영미,최창현,김경훈,김경민,박태일,강택규,강성욱,박철수,조성우 한국육종학회 2019 한국육종학회지 Vol.51 No.1

The flour physiochemical properties and end-use quality of wheat were evaluated to identify the effect of high temperature (HT) during grain maturation. HT caused a decrease in the width and thickness of grains. However, HT did not affect the grain weight, volume, length, test weight and 1,000-kernel weight in Korean wheat cultivars. Although HT marginally affected the general flour physiochemical properties and gluten composition, the effects were not significant. Based on the results of the evaluation of end-use quality, the effect of HT on the end-use quality was not significant. Nevertheless, the allelic composition was related to the quantity and quality of grain under HT conditions. In Korean wheat cultivars containing Glu-D1d and -D1f alleles, the 1,000-kernel weight was decreased by HT. In addition, Glu-D1f was more sensitive to HT than was Glu-D1d. The proportion of the y-type high-molecular-weight glutenin subunits (HMW-GSs) was decreased by HT in Korean wheat cultivars containing the Glu-A1ab allele, and was increased in cultivars containing the Glu-B3d allele. Furthermore, the proportions of gliadin and low-molecular-weight glutenin subunits (LMW-GSs) were decreased by HT in Korean wheat cultivars containing Glu-A1ab or Glu-B3ahi alleles.

借用語の音韻構造とアクセント

孫在賢(손재현, Son, Jae-Hyun) 일본어문학회 2009 일본어문학 Vol.44 No.-

The present paper examines on the basis of the data assembled by the author the conclusion by Kenstowicz & Sohn (2001) that the accent of loanwords in the Gyeongsang dialect is determined by their syllabic structure. As a result it is shown that the highest average of the application of the K & S's principle is about 70% as regards three or four syllable words. This average is only achieved when the following two conditions regarding the original word of borrowing are taken into account. There is no correlation between the syllabic structure and accent as regards monosyllabic words, since the accent remains the same even if the syllabic structure is altered by the addition of a particle. The present paper thus critically examines the applicability of the two conditions concerning the segmental structure of the original word, the prerequisites of the discussion of K & S (2001), as well as the level of knowledge of the original words in the acquisition of loanwords by the dialect speakers.

RAPD 마커를 이용한 한국밀의 유전적 다양성 평가 및 품종 판별

손재한,정영근,박종철,김양길,박종호,김경호,박태일,김보경,강천식 한국육종학회 2017 한국육종학회지 Vol.49 No.2

Eleven RAPD primers were assessed to analyze genetic diversity of Korean wheat varieties and to develop DNA marker for cultivar identification. The average of the number of polymorphic bands was 5.2 and PIC values showed 0.48, respectively. Ten major clades were presented by phylogenetic analysis. Three cultivars containing Uri, Hanbeak and Jonong were distinct from the others in the phylogenetic dendrogram. Seven cultivar-specific fragments were detected from 11 RAPD fingerprinting among 35 wheat cultivars and they were sequenced. Four Korean wheat cultivars, Eunpa, Jopoom, Yeonbaek and Jeokjoong, were identified newly by four markers, 84, 173, 174 and KWSM011. We convince that these new DNA markers are useful for cultivar fingerprinting and are applied to marker-assisted selection in wheat breeding program.