한국 재래산양의 태아 및 신생아의 폐 발달에 관하여

서득록 ( Deuk Lok Seo ),이국천 ( Kuk Cheon Lee ),이순선 ( Soon Sun Lee ),김종섭 ( Chong Sup Kim ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1

The morphological development of lungs in fetuses of 60, 90 and 120 days of gestation and neonates of Korean native goats was investigated by light, scanning and transmission electron mictroscope. The results were summarized as follows; 1. Gross findings: In the 60-days-old fetus, the lung was developed and differentiated into six lobes. 2. Light microscopic findings: The gland-like bronchioles were formed in loose mesenchyme at 60 days of gestation and the bronchial wall contained smooth muscles. The loose mesenchyme had been replaced by compact parenchymal tissue at 90 days of gestation and the cartilage plates appeared in bronchial wall which contained blood vessels, submucosal glands and smooth muscles. The lung parenchyma consisted of a fine network of alveoli at 120 days of gestation and the bronchial wall contained well-developed blood vessels, submucosal glands, cartilage plates and smooth muscles. In neonates, the lung tissue was similar to the mature lung tissue and the bronchial wall contained well developed cartilage plates. 3. Scanning electron microscopic findings: The epithelial cells lining the tubules were composed of cuboidal or columnar at 60 days of gestation and the epithelial cells lining the large airways were often ciliated; some were covered with stubby microvilli. The epithelial cells lining the canals were cuboidal at 90 days of gestation and the epithelial cells lining the bronchioles were ciliated cells or nonciliated(clara) cells, The clara cells contained row microvilli. The alvealor development of this stage was rapidly progressed; the subdivision of canals by alveolar crests and assosiated wall attenuation resulted alveoli at 120 days of gestation and the respiratory bronchioles were lined by ciliated or nonciliated epithelial cells. In neonates, the epithelial cells lining the alveolar walls were mainly covered with pneumocyte type Ⅰ; Some were covered with pneumocyte type Ⅱ. 4. Transmission electron microscopic findings: The epithelial cells lining the tubules were adhered with tight junction at apical borders of the adjacent cells at 60 days of gestation, which contained few organells and glycogen. The epithelial cells lining the canals were composed mostly of cuboidal cell at 90 days of gestation and the epithelial cells lining of the bronchioles were ciliated of nonciliated cell, which contained few organelles and abundant glycogen. The epithelial cells lining the alveolar walls were composed of pneumocyte type I and a few pneumocyte type Ⅱ at 120 days of gestation. The epithelial cells lining of the bronchioles were ciliated or nonciliated cells. In neonates, pneumocyte type I was observed as flat and thin cytoplasmic extension in shape. Otherwise, pneumocyte type Ⅱ was observed as cuboidal type with apical microvilli and contained osmiophillic lamellar inclusion bodies. Putting these various experiment results together, the lung development was slowly progressed at early stage, which was rapidly progressed in the late stage of gestation.

경남(慶南) 북부지역(北部地域)에서 도축(屠畜)된 한우(韓牛)의 간질(肝蛭) 감염율(感染率)과 혈액성장(血液性壯)의 변화(變化)에 관한 연구(硏究)

서득록 ( Deuk Lok Seo ),이국천 ( Kuk Cheon Lee ),이순선 ( Soon Sun Lee ),김종수 ( Jong Shu Kim ),김충희 ( Chung Hee Kim ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.2

An abattoir survey was carried out on 300 Korean native cattles reared and slaughtered in North Kyeongnam area to determine the prevalence of Fasciola hepatica infections. The average prevalence of fascioliasis was 46.4%; the infection rate had a tendency to increase with age. Some hematological and biochemical indices were assayed in Korean native cattle naturally harbouring Fasciola hepatica infection and compared with uninfection control. Affected animal revealed reduction total erythrocyte count and homoglobin, which was consistent in total leucocyte count and packed cell volume. Infected cattle had lower level of serum total protein and albumin, was consistent serum calcium and glucose indices, had higher levels of sGOT, sGPT and choresterol indices.

Diethylnitrosamine을 투여한 rat 간장의 tumorigenesis에 관하여 3. 간장조직의 전자현미경적 관찰

곽수동,김종섭,고필옥,양재훈,서득록,Kwak, Soo-dong,Kim, Chong-sup,Koh, Phil-ok,Yang, Je-hoon,Seo, Deuk-lok 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.6

The study was designated to investigate the electron microscopic findings following diethylnitrosamine (DEN) treatment in rats. Forty four male (Srague Dawley) rats were continuously given water containing 0.01% DEN for 13 weeks and livers of five rats with more tumor lesions at 16 and 17 weeks after initial treatment were used as EM materials. In transmission electron microscopic findings, most small-sized hepatocytes were active cells containing large mount of organelles, but light (pale staining) hepatocytes among small-sized hepatocytes were injured cells containg disorganized organelles. Tumor cells among small-sized hepatocytes were irregularly arranged and have pleomorphic nuclei containing electron dense chromatin but the organelles in cytoplasm were swelled. Large-sized hepatocytes were active cells with condensed chromatin but the cytoplasm of these cells were pale due to be injured and dilated organelles. Dark hepatocytes were apoptotic cells with homogenous pyknotic nuclei and cytoplasm, and the cytoplasm of these cells contained dilated smooth endoplasmic reticulum (sER) but these sER were non-vesiculated. Cholangiocarninoma cells were crowded and were pale by far less number of organelles in cytoplasm and nuclei. In scanning electron microscopic findings, the lumens of portal veins, bile canaliculi, bile ductules, bile ducts and sinusoids were dilated and have irregular folded inner surface by protruded parenchyma.

경남지방의 도태우에 불임과 관련된 난소의 형태학적 관찰 : 2. 난소낭종의 발생과 낭종형태에 대하여 2. Incidences and Morphological Findings of Ovarian Cysts

곽수동,표병민,양재훈,김철호,고필옥,강정부,서득록 한국임상수의학회 2002 한국임상수의학회지 Vol.19 No.2

Ovaries from total 192 slaughtered cows(154 Korean native cows and 38 Holstein cows) were collected during the slaughtering process in Kimhae, Changyoung and Yangsan abattoirs in Kyungnam province from January 2001 to January 2002. In order to investigate incidence of the ovarian cysts, anatomical, histological observations were performed and also TUNEL methods and PCNA antibody by immunohistochemical methods for diagnostic accuracy of cysts in a few ovaries were applied. Apoptotic positive cells by TUNEL method appeared not or a few in cystic walls but appeared more number in normal large follicular walls and the proliferative positive cells by PCNA antibody appeared numerous in normal large follicular walls but not or a few in cystic walls. The incident rates of ovarian cysts were 19.5% in Korean native cows and 18.4% in Holstein cows. The incident rates of ovarian cysts in Holstein cows were lower than that of Koran native cows. The incident rates of follicular cysts and luteal cysts in Korean native cows were 11.7% and 7.8% respectively. The incident rates of follicular cysts and luteal cysts in Holstein cows were 10.5% and 7.9%, respectively. Higher incidence proportions of ovarian cysts according to seasons in Korean native cows were ordered as spring (29.8%), autumn (21.4%) winter (14.3%) and summer (6.7%). Rates of cows with single cyst and multiple cysts were 63.3%(19 heads /30 heads) and 36.7%(11 heads/30 heads) in 30 cystic Korean native cows, respectively. Cystic cows with corpus luteums were 50.0%(15 heads) in 30 Korean native cows and 42.9%(3 heads) in 7 dairy cows, respectively. Among 15 cystic Korean native cows with corpus luteums, rates of cows with single corpus luteum were 66.7%(10 heads) and rates of multiple corpus luteum were 33.3%(5 heads ), respectively. The average diameter of cysts and corpus luteums in cystic ovaries were 21.0×17.1 ㎜ and 18.1×13.8 ㎜ in 30 Korean native cows and 20.6×17.7 ㎜ and 19.3×14.9 ㎜ in 7 Holstein cows, respectively. So the average sizes of cysts in cystic ovaries were larger than those of corpus luteums.