Characterization of Recombinant β-Glucosidase from Arthrobacter chlorophenolicus and Biotransformation of Ginsenosides Rb1, Rb2, Rc, and Rd

박명근,Chang-Hao Cui,Sung Chul Park,박슬기,김진광,Mi-Sun Jung,정석채,김선창,임완택 한국미생물학회 2014 The journal of microbiology Vol.52 No.5

The focus of this study was the cloning, expression, andcharacterization of recombinant ginsenoside hydrolyzingβ-glucosidase from Arthrobacter chlorophenolicus with anultimate objective to more efficiently bio-transform ginsenosides. The gene bglAch, consisting of 1,260 bp (419 aminoacid residues) was cloned and the recombinant enzyme, overexpressedin Escherichia coli BL21 (DE3), was characterized. The GST-fused BglAch was purified using GST·Bind agaroseresin and characterized. Under optimal conditions (pH 6.0and 37°C) BglAch hydrolyzed the outer glucose and arabinopyranosemoieties of ginsenosides Rb1 and Rb2 at the C20position of the aglycone into ginsenoside Rd. This was followedby hydrolysis into F2 of the outer glucose moiety ofginsenoside Rd at the C3 position of the aglycone. Additionally,BglAch more slowly transformed Rc to F2 via C-Mc1(compared to hydrolysis of Rb1 or Rb2). These results indicatethat the recombinant BglAch could be useful for theproduction of ginsenoside F2 for use in the pharmaceuticaland cosmetic industries.

효율적인 엘리베이터 제어를 위한 로봇용 버튼 인지 및 눌림 판별 알고리즘

박명근(Myeonggeun Park),이학준(Hakjun Lee) 한국통신학회 2023 한국통신학회 학술대회논문집 Vol.2023 No.11

년초과치(年超過値) 계열(系列)의 홍수빈도(洪水頻度) 분석(分析) 관(關)한 연구(硏究) - 금강(錦江) 유역(流域)을 중심(中心)으로 -

박명근 ( Myeong Keun Park ),이순혁 ( Soon Hyuk Lee ) 한국농공학회 1982 韓國農工學會誌 : 전원과 자원 Vol.24 No.1

This study was attempted to find best fitted distribution and the equations for probable maximum flow with the evaluation of parameters by the method of moment for the rat-ional design of hydraulic structures in the annual exceedance series. Six subwatersheds were selected as studying basins along Geum River basin. The results obtained through this study were analyzed and summarized as follows. 1. Fitted probability distribution was showed in the order of Three Parameter Lognorm-al, Type 1 Extremal, Exponential, Pearson Type III, and Log Pearson Type III distribu-tion as the results of x² goodness of fit test. 2. Kolmogorov-Smirnov test showed in the order of Three Parameter Lognormal, Exp-onential' Pearson Type III, Log Pearson Type III and Type 1 Extremal distribution for the fitted probability distribution. 3. It can be concluded that Three parameter Lognormal distribution is a best fitted one among some other distributions out of respect for each both tests. 4. An Exponential distribution was proposed as a suitable one by Chow, V.T. showed lower fittness than that of Three Parameter Lognormal in Geum River basin. 5. Probable flood flow equations followins the return periods for each station were obt-ained by Three Parameter Lognormal distribution. 6. It is urgently essential that best fitted probability distribution should be established for the annual exceedance series in the main river systems of Korea.

건설기계부품연구원 건설기계 · 산업기계 연구용 수소충전소 소개

박명근(Myunggeun Park),김희수(Heesu Kim) 유공압건설기계학회 2023 드라이브·컨트롤 Vol.20 No.2

월유출량의 모의발생에 관한 비교 연구

박명근 ( Park Myeong Keun ),서승덕 ( Suh Seung Duk ),이순혁 ( Lee Soon Hyuk ),맹승진 ( Maeng Sung Jin ) 한국농공학회 1996 韓國農工學會誌 : 전원과 자원 Vol.38 No.4

This study was conducted to simulate long series of synthetic monthly flows by multi-season first order Markov model with selection of best fitting frequency distribution, harmonic synthetic and harmonic regression models and to make a comparison of statistical parameters between observes and synthetic flows of five watersheds in Geum river system. The results obtained through this study can be summarized as follow. 1. Both gamma and two parameter lognormal distributions were found to be suitable ones for monthly flows in all watersheds by Kolmogorov-Smirnov test. 2. It was found that arithmetic mean values of synthetic monthly flows simulated by multi-season first order Markov model with gamma distribution are much closer to the results of the observed data in comparison with those of the other models in the applied watersheds. 3. The coefficients of variation, index of fluctuation for monthly flows simulated by multi-season first order Markov model with gamma distribution are appeared closer to those of the observed data in comparison with those of the other models in Geum river system. 4. Synthetic monthly flows were simulated over 100 years by multi-season first order Markov model with gamma distribution which is acknowledged as a suitable simulation modal in this study.

2 톤급 전동식 굴착기 적용 에너지 회생 시스템 개발

박상균(S. Park),김희수(H. Kim),정주영(J. Jeong),박명근(M. Park),김준석(J. Kim),김재덕(J. Kim),유한성(H. S. Yoo),진원준(W. Jin) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.5월

코로나19를 계기로 자연/생태계 보전을 포함해 지속 가능한 국가 발전전략의 중요성이 부각되고 있다. 세계 주요 선진국들은 넷-제로(탄소 순 배출 제로)를 선언, 저탄소 경제 선도전략으로 그린뉴딜을 제시하는 등 기후위기 대응노력을 강화하고 있다. 이러한 추세 속 지금까지 자동차에 집중되어 온 친환경화는 화물차, 상용차, 건설기계 등 다양한 모빌리티로 확대되고 있다. 그 일환으로 노후 경유차 및 건설기계 116 만대를 조기 폐차하고 친환경 모빌리티로 전환하는 정책이 정부로부터 시행되고 있고, 친환경 모빌리티의 효율을 추가 개선하는 연구가 진행 중이다. 본연구의 목적은 에너지 회생 시스템을 전동식 굴삭기에 적용하여 시스템 효율을 높이는 것에 있다. 연구에 적용된 에너지 회생 시스템은 굴착기 선회 사이클 모드 작업 시 발생되는 에너지 중 실제 작업에 필요한 전력을 초과하는 잉여 에너지를 슈퍼캐패시터에 저장한다. 이후, 저장한 에너지를 굴삭기의 향후 선회 동작 시 모터 구동에 활용하는 것으로 시스템 운영을 최적화하였다. 연구 결과 선회 작업 중 파워팩에서 발생한 에너지의 일정 부분이 실제 작업에 사용되고, 필요한 요구 전력을 초과한 잉여분은 회생 시스템을 통해 슈퍼캐패시터에 저장된다. 굴착기의 추가 선회 동작 시 저장된 전력을 활용함으로써 시스템 효율이 개선되었다.

水利構造物의 破壞危險度와 設計洪水量에 관한 水文學的 硏究(Ⅰ) -年最高値 系列을 中心으로-

이순혁,박명근,Lee, Soon-Hyuk,Park, Myeong-Keun 한국농공학회 1985 한국농공학회논문집 Vol.27 No.2

This studies were carried out to get characteristics of frequency distribution, probable flood flows according to the return periods, and the correlation between return periods and those length of records affect the Risk of failure in the annual maximum series of the main river systems in Korea. Especially, Risk analysis according to the levels were emphasized in relation to the design frequency factors for the different watersheds. Twelve watersheds along Han, Geum, Nak Dong, Yeong San and Seom Jin river basin were selected as studying basins. The results were analyzed and summarized as follows. 1. Type 1 extremal distribution was newly confirmed as a good fitted distribution at selected watersheds along Geum and Yeong San river basin. Three parameter lognormal Seom Jin river basin. Consequently, characteristics of frequency distribution for the extreme value series could be changed in connection with the watershed location even the same river system judging from the results so far obtained by author. 2. Evaluation of parameters for Type 1 extremal and three parameter lognormal distribution based on the method of moment by using an electronic computer. 3. Formulas for the probable flood flows were derived for the three parameter lognormal and Type 1 extremal distribution. 4. Equations for the risk to failure could be simplified as $\frac{n}{N+n}$ and $\frac{n}{T}$ under the condition of non-parametric method and the longer return period than the life of project, respectively. 5. Formulas for the return periods in relation to frequency factors were derived by the least square method for the three parameter lognormal and Type 1 extremal distribution. 6. The more the length of records, the lesser the risk of failure, and it was appeared that the risk of failure was increasing in propotion to the length of return periods even same length of records. 7. Empirical formulas for design frequency factors were derived from under the condition of the return periods identify with the life of Hydraulic structure in relation to the risk level. 8. Design frequency factor was appeared to be increased in propotion to the return periods while it is in inverse proportion to the levels of the risk of failure. 9. Derivation of design flood including the risk of failure could be accomplished by using of emprical formulas for the design frequency factor for each watershed.

수리구조물(水利構造物)의 파괴위험도(破壞危險度)와 설계홍수량(設計洪水量)에 관한 수문학적(水文學的) 연구(硏究)(I)- 년최고치(年最高値) 계열(系列)을 중심(中心)으로 -

이순혁 ( Lee Soon Hyuk ),박명근 ( Park Myeong Keun ) 한국농공학회 1985 韓國農工學會誌 : 전원과 자원 Vol.27 No.2

This studies were carried out to get characteristics of frequency distribution, probable flood flows according to the return periods, and the correlation between return periods and those length of records affect the Risk of failure in the annual maximum series of the main river systems in Korea. Especially, Risk analysis according to the levels were emphasized in relation to the design frequency factors for the different watersheds. Twelve watersheds along Han, Geum, Nak Dong, Yeong San and Seom Jin river basin were selected as studying basins. The results were analyzed and summarized as follows. 1. Type 1 extremal distribution was newly confirmed as a good fitted distribution at selected watersheds along Geum and Yeong San river basin. Three parameter lognormal Seom Jin river basin. Consequently, characteristics of frequency distribution for the extreme value series could be changed in connection with the watershed location even the same river system judging from the results so far obtained by author. 2. Evaluation of parameters for Type 1 extremal and three parameter lognormal distribution based on the method of moment by using an electronic computer. 3. Formulas for the probable flood flows were derived for the three parameter lognormal and Type 1 extremal distribution. 4. Equations for the risk to failure could be simplified as {n} over {N+m} and {n} over {T}under the condition of non-parametric method and the longer return period than the life of project, respectively. 5. Formulas for the return periods in relation to frequency factors were derived by the least square method for the three parameter lognormal and Type 1 extremal distribution. 6. The more the length of records, the lesser the risk of failure, and it was appeared that the risk of failure was increasing in propotion to the length of return periods even same length of records. 7. Empirical formulas for design frequency factors were derived from under the condition of the return periods identify with the life of Hydraulic structure in relation to the risk level. 8. Design frequency factor was appeared to be increased in propotion to the return periods while it is in inverse proportion to the levels of the risk of failure. 9. Derivation of design flood including the risk of failure could be accomplished by using of emprical formulas for the design frequency factor for each watershed.

Gamma 및 Generalized Gamma 분포 모형에 의한 적정 설계홍수량의 유도(I) -Gamma 분포 모형을 중심으로-

이순혁 ( Lee Soon Hyuk ),박명근 ( Park Myeong Keun ),정연수 ( Jeong Youn Su ),맹승진 ( Maeng Sung Jin ),류경식 ( Ryoo Kyong Sik ) 한국농공학회 1997 韓國農工學會誌 : 전원과 자원 Vol.39 No.3

This study was conducted to derive optimal design floods by Gamma distribution models of the annual maximum series at eight watersheds along Geum, Yeong San and Seom Jin river systems. Design floods obtained by different methods for evaluation of parameters and for plotting positions in the Gamma distribution models were compared by the relative mean errors and graphical fit along with 95% confidence interval plotted on Gamma probability paper. The results were analyzed and summarized as follows. 1. Adequacy for the analysis of flood flow data used in this study was confirmed by the tests of Independence, Homogeneity and detection of Outliers. 2. Basic statistics and parameters were calculated by Gamma distribution models using Methods of Moments and Maximum Likelihood. 3. It was found that design floods derived by the method of maximum likelihood and Hazen plotting position formular of two parameter Gamma distribution are much closer to those of the observed data in comparison with those obtained by other methods for parameters and for plotting positions from the viewpoint of relative mean errors. 4. Reliability of derived design floods by both maximum likelihood and method of moments with two parameter Gamma distribution was acknowledged within 95% confidence interval.

로봇 매니퓰레이터를 위한 객체의 3차원 위치 추정 및 객체 조작 기법

진실(Sil Jin),박명근(Myeonggeun Park),송지민(Jimin Song),진용식(Yongsik Jin),이상준(Sang Jun Lee) 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.11