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갑상선암에서 MAGE 1-6 mRNA를 검출하는MAGE common primer의 의의
남철우,최영식,박요한,이강대 고신대학교의과대학 2006 고신대학교 의과대학 학술지 Vol.21 No.2
Background: MAGE (melanoma antigen gene) was named as cancer/testis specific antigens since their expression is only detected in the testis or cancer cells. Because of their relatively specific expression in the cancer cells, they have been considered as the early diagnosis of several cancers, or the appropriate targets for the specific immunotherapy mediated by cytotoxic T lymphocytes. Therefore, there were many reports about the expression of MAGE genes in various types of malignant tumors, but only a few reports in human thyroid neoplasms. The purpose of this study was to determine whether the MAGE common primers expressed in different histological types of thyroid tumors and to see the clinical usefulness of MAGE common primers on the thyroid tumors. Methods: Fifty-nine patients who underwent thyroidectomy at Kosin Medical Center from January, 2001 to April, 2002 were included in the study. Of the 59 patients 40 were papillary carcinoma, 4 follicular carcinoma, 3 medullary carcinoma, 1 anaplastic carcinoma, 4 nodular hyperplasia, 3 adenomatous goiter, and 4 follicular adenoma. Total cellular mRNA was extracted from those 48 cancer tissues, 11 benign tissues and additional eleven normal control thyroid tissues. Nested reverse transcriptase-polymerase chain reactions (nested RT-PCR) with the MAGE common primers designed to detect MAGE 1-6 genes were employed in this study. With probes confirmed by DNA sequencing. Results: Of those 48 cases of thyroid carcinomas 10 cases (20.8%) were expressed MAGE common primers. In the 40 papillary carcinoma, MAGE common primers were expressed in 17.5%. In the four follicular carcinoma, they were expressed in two cases, and in one case of one anaplatic carcinoma. However, in the three cases of medullary carcinoma, MAGE gene was not expressed. None of 11 benign tumors and 11 normal tissues expressed any of these MAGE common primers. The sensitivity of MAGE common primers in thyroid tumors was 20.8% and the specificity was 100%. Conclusion: These results suggested that MAGE common primers were expressed low in the malignant thyroid tumors but it was not expressed in benign tumors and normal tissues. It is suggested that MAGE common primers can be used for preoperative differential diagnosis of follicular carcinoma from benign follicular adenoma which cannot be made by fine needle aspiration cytology of thyroid tissue. However, further study is needed for clinical application of MAGE common primers expression as tumor markers in the thyroid tumors.
남철우,한광래,송판섭 한국초등과학교육학회 1997 초등과학교육 Vol.16 No.1
The purpose of this study was to develop some supplementary experiments to forster scientific inquiring ability of student in primary school. The developed subjects of experiments are selected to be expected that students have more scientific interest and curiosity, closely connected with the contents of the natural science by the sixth national curriculum. The subjects of the developed supplementary experiments are 1) Heating the powder of silver iodide and nikel sulfate, 2) Making multilayered liquid, 3) Crystal growth of NiSO₄, CuSO₄, NaCI, 4) The gaseous acid-base reaction, 5) Volume change with mixing of acetone-carbondisulfide, 6) Combustion of candle.
제6차와 제7차 교육과정에서 초등학교 과학 교과서 삽화 비교 분석 : Centered on the 3rd Year Class of the Elementary School
남철우,권영길 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.1
This study was intended to examine the difference and the suggestion through comparative analysing the illustrations in the 3rd elementary science textbooks of the 6th with the 7th curriculum. The findings of this research were as follows. (1) Compared with the 6th curriculum, 448 more illustrations are presented in the 7th curriculum science textbook and the teacher's teaching techniques should be, therefore, improved the using ability of the illustration in the science education. (2) Although more than 96% of the illustrations are those of photographs and pictures in the 6th curriculum, those in the 7th consist of many kinds of the illustrations-photographs, picture, diagrams and cartoons. Especially, the more frequent introductions of the cartoons in the textbook give familiarity to the students, and it suggests that the textbooks were constructed in consideration of the student's intellectual development. (3) The roles of illustrations in the 6th and the 7th curriculum are mostly guidance for experiments and the supply of the learning materials. but considering that the learning motivation is very important in the teaching-learning process. the percentage of the illustrations in accordance with the motivations must be increased, (4) As the result of the analysis of illustration in the each domains, in the 6th curriculum there are the most numbers of illustrations in the biology domain and the least in the physics domain. But contrastively in the 7th curriculum there are the most in the energy and the least in physics, and namely those are distributed widely. (5) Analysing the kinds of illustrations in the each domains, in the both sixth and seventh curriculum the photographs are used in the physics mostly, and it is, therefore, interpreted because of the reason that the more realistic material supply should be needed. (6) According to the roles of illustrations in the each domains, there are the many illustrations for guidance of the experiments in the both curriculum, and contrastively in the biology part and the earth science, the many illustrations are introduced for material supply. In this viewpoint it is known that the illustrations of the physics domains and the energy are constructed for experiment, and those of the biology domain and earth science for investigation and observation of the given materials. (7) the average numbers of illustrations to be taught in a period or in a page are introduced more twice in the sixth text-book than the seventh. It is, naturally, concluded that the teachers' training process for using the illustrations is needed urgently.
환원배소-용융에 의한 망간단괴로부터 코발트, 니켈, 구리 회수
남철우,김병수,박경호 한국자원공학회 2003 한국자원공학회지 Vol.40 No.3
Recovery of cobalt, nickel, and copper from manganese nodules by reduction roasting and smelting was thermodynamically investigated. Manganese nodules are dried under air atmosphere and reduced with carbon as a reductant under nitrogen atmosphere. The nodules were then melted at 1693 K for 1 hr under nitrogen atmosphere. The melting products were CaO-MnO-SiO2 slag and Fe-Cu-Co-Ni alloy. From the experimental results, it was found that the recovery of cobalt, nickel, and copper increased with the manganese content in the product alloy. That is the reason manganese can oxidize the easiest among metal components in the product alloy. Also, an experimental equation to calculate necessary amount of carbon as the reductant for recovering up to 90.0 % for cobalt, nickel, and copper respectively in Fe-Cu-Co-Ni alloy containing below 5.0 wt% Mn from the nodules was obtained as follows;X wt% = 0.0728*Mn wt% + 0.0899*Cu wt% + 0.2016*Ni wt% + 0.2000*Co wt% + 0.3226*Fe wt% 심해저 망간단괴로부터 환원배소-용융법에 의한 코발트, 니켈, 구리의 분리에 대한 열역학적인 연구가 수행되었다. 사용된 망간단괴는 대기 분위기에서 건조된 후, 질소 분위기에서 환원제 탄소에 의하여 환원배소된 다음, 1693 K로 가열하여 질소분위기에서 1시간 동안 용융되었다. 용융 후 CaO-MnO-SiO2 슬래그와 Fe-Cu-Co-Ni 합금이 생성되었다. 실험결과 코발트, 니켈, 구리의 회수율은 생성된 합금상의 망간함유량이 높을수록 증가하는 것으로 나타났다. 이것은 생성된 합금상에 있는 성분들의 산화력 중 망간의 산화력이 열역학적으로 월등히 크기 때문이다. 생성된 합금중의 망간함유량이 5 wt%이하이면서, 망간단괴로부터 90.0 % 이상의 코발트, 니켈, 구리를 각각 회수하기 위하여 필요한 탄소량을 계산하기 위한 실험식이 아래와 같이 얻어졌다.X wt% = 0.0728*(Mn wt%) + 0.0899*(Cu wt%) + 0.2016*(Ni wt%) + 0.2000*(Co wt%) + 0.3226*(Fe wt%)