성숙정도에 따른 찰/초당옥수수의 주요특성과 당함량의 변화

김익휘,김순권,이상철 한국작물학회 1999 한국작물학회 학술발표대회 논문집 Vol.1999 No.-

화생방 무기체계의 변천사와 향후 발전방향

김익휘 한국방위산업진흥회 2016 國防과 技術 Vol.- No.444

[감동적인 합격기: 2023년도 5급 공채 일반행정직 합격] “난 올해 붙는다”라는 마인드로

김익휘 고시계사 2024 考試界 Vol.69 No.3

잔디밭 잡초 바랭이와 민들레 방제를 위한 살초활성 약용식물의 탐색

김대호,김익휘,김인섭,전민구,이재득,강재영 한국잔디학회 2012 Weed & Turfgrass Science Vol.26 No.1

Crabgrass (Digitaria sanguinalis L.), Dandelion (Taraxacum platycarpum H. DAHLST.), Annual bluegrass (Poa annua L.), Gree kyllinga (Kyllinga brevifolia var.), Red clover (Trifolium pratense L.), Annual lespedeza (Kummerowia striata (Thunb.)Schindl.), Mugwort (Artemisia capillaris Thunb.), Horseweed (Erigeron canadensis L.), Field horsetail (Equisetum arvense L.) are major weeds in turf. In this study, 23 medicinal plant extracts were tested for herbicidal activities against crabgrass and dandelion in the course of major weed in turf. In pot experiment, we implemented pre-emergence application used medicinal plant extracts showed herbicidal activity in petridish experiment. In effect, inhibition rates of germination were 61.0% at Curcuma longa extract and 62.5% at Cnidium officinale extract in crabgrass pot experiment, and 77.8% at Ailanthus altissima extract in dandelion pot experiment. 본 연구에서는 잔디밭 문제 잡초인 바랭이(Digitaria sanguinalis L.)와 민들레(Taraxacum platycarpum H. DAHLST.)에 대한 약용식물 추출물 23종의 살초활성을 조사하였다. 페트리디쉬 검정에서 효과가 나타난 약용식물추출물을 선발하여 포트에서 발아전 처리를 하였으며, 그결과 바랭이에서는 울금(61%)과 천궁(62.5%) 추출물에서발아억제 효과가 나타났으며, 민들레에서는 가죽나무(77.8%)추출물에서 발아억제 효과가 나타났다. 경엽처리에서는 두초종 모두 가죽나무 추출물에서 살초효과가 우수한 것으로 나타났다.

Morinda citrifolia Leaf Extract Enhances Osteogenic Differentiation Through Activation of Wnt/β-Catenin Signaling

구한나,Kanitsak Boonanantanasarn,강문규,김익휘,우경미,류현모,백정화 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.1

Morinda citrifolia (Noni) leaf is an herbal medicine with application in the domestic treatment of a broad range of conditions, including bone fracture and luxation. However, the basic mechanism underlying the stimulation of osteogenic differentiation by Noni leaf extract remains poorly understood. This study aimed to examine the effect of this extract on osteogenic differentiation and the mechanism by which Noni leaf extract enhances osteogenic differentiation. Aqueous extract of Noni leaves was prepared, and rutin and kaempferol-3-O-rutinoside were identified to be two of its major components. C2C12 and human periodontal ligament (hPDL) cells were used to study the effect of Noni. Noni did not show cytotoxicity at a concentration range of 0.015%–1.0% (w/v%) and significantly enhanced the activity of alkaline phosphatase (ALP) and expression levels of osteoblast differentiation markers, including Runx2, ALP, osterix, and osteocalcin, bone morphogenetic protein 2, Wnt3a, and β-catenin. In addition, Noni enhanced the matrix mineralization of hPDL cells. In the signaling pathways, Noni increased the phosphorylation levels of Akt and GSK3β and nuclear translocation and transcriptional activity of β-catenin, which were attenuated by the addition of Dkk-1, a Wnt inhibitor, or LY294002, a PI3K inhibitor. These results suggest that Noni leaf extract enhances osteogenic differentiation through the PI3K/Akt-dependent activation of Wnt/β-catenin signaling. Noni leaf extract might be a novel alternative medicine for bone and periodontal regeneration in patients with periodontal diseases.

잔디 병해 방제를 위한 항균성 약용식물의 탐색

권수민,김대호,장태현,전민구,김인섭,김익휘 한국잔디학회 2010 Weed & Turfgrass Science Vol.24 No.2

Brown patch (Rhizoctonia solani AG1-1), dollar spot (Sclerotinia homoeocarpa), pythium blight (Pythium spp.), anthracnose (Colletotrichum graminicola), yellow patch (Rhizoctonia cerealis) and zoysia patch (Rhizoctonia solani AG2-2) are the major turfgrasses diseases in Korea. In this study, 23 medicinal plant extracts were tested for antifungal activities against turfgrass pathogenic fungi. In paper disk diffusion method, 12 medicinal plant extracts, including Sophora flavescens, showed antifungal activity. Also, in the test of antifungal activity on media contained the extracts of S. flavescens, Curcuma longa, Rheum undulatum, Coptis chinensis and Asiasarum sieboldi showed above 80% inhibitory effects on the mycelial growth in 110 mg/10 ml concentration of the extracts. S. flavescens, in particularly, showed antifungal activity against the six turfgrass pathogenic fungi. The inhibition rate of S. homoeocarpa was 100% in 10 mg/10 ml, 5 mg/10 ml and 2 mg/10 ml concentrations of C. longa extract. In case of Pythium spp., the extracts of S. flavescens, R. undulatum and C. chinensis showed 100 % inhibition rate on the test media.

잔디 병해 방제를 위한 약용식물의 항균작용 탐색

강재영,김대호,이동구,김인섭,전민구,이재득,김익휘,이상현 한국잔디학회 2013 Weed & Turfgrass Science Vol.2 No.1

Seven medicinal plant extracts were tested for antifungal activities against six species of the major turfgrass pathogenic fungi (Colletotrichum graminicola, Pythium spp., Rhizoctonia cerealis, Rhizoctonia solani AG1-1, Rhizoctonia solani AG2-2, and Sclerotinia homoeocarpa) using paper disk diffusion method. Three medicinal plant extracts, including Pinus densiflora showed antifungal activities. In suppression of mycelium growth test, on medium adding P. densiflora extract showed that inhibition rate of mycelium growth were above 80% in 10 mg/10 ml concentration of the extract. The inhibition rate of Pythium spp. was 100% and C. graminicola was 84.3% in 10 mg/10 ml concentrations of P. densiflora extract, respectively. In particularly, the inhibition rate of Pythium spp. was 89.5% in 2 mg/10 ml concentrations of P. densiflora extract. As a result, P. densiflora extract showed high antifungal activity to Pythium spp. and C. graminicola of the turfgrass pathogen in in vitro test. 본 연구에서는 7종의 약용식물(송절, 지구목, 측백, 대풍자, 유백피, 은행잎, 참오동나무)을 사용하여 잔디의 주요병원균 6종(R. solani AG 2-2(IV), R. solani AG-1 IB,Pythium sp., S. homoeocarpa, R. cerealis, C. graminicola)을대상으로 항진균 활성을 실험하였다. 디스크 확산법으로항진균 활성이 있는 약용식물 추출물을 선발하였고, 선발된 추출물을 사용하여 균사생육 억제 효과를 검정하였다. 디스크 확산법으로 실험 한 결과 7종의 약용식물 중 송절,측백, 유백피 등 3종의 추출물에서 항진균 활성이 있는 것으로 나타났다. 송절 추출물 1,000배 처리에서 Pythium sp. 에 대하여 100%, C. graminicola에 대하여 84.3%의 균사생육 억제율을 나타내었으며, 특히 Pythium sp.에 대해서는 5,000배 처리에서도 89.5%의 균사생육 억제율을 나타내었다.

잔디밭 잡초 바랭이와 민들레 방제를 위한 살초활성 약용식물의 탐색

김대호,강재영,김인섭,전민구,이재득,김익휘,Kim, Dae-Ho,Kang, Jae-Young,Kim, In-Seob,Jeon, Min-Goo,Lee, Jae-Deuk,Kim, Ik-Hwi 한국잔디학회 2012 한국잔디학회지 Vol.26 No.1

본 연구에서는 잔디밭 문제 잡초인 바랭이(Digitaria sanguinalis L.)와 민들레(Taraxacum platycarpum H. DAHLST.)에 대한 약용식물 추출물 23종의 살초활성을 조사하였다. 페트리디쉬 검정에서 효과가 나타난 약용식물 추출물을 선발하여 포트에서 발아전 처리를 하였으며, 그 결과 바랭이에서는 울금(61%)과 천궁(62.5%) 추출물에서 발아억제 효과가 나타났으며, 민들레에서는 가죽나무(77.8%) 추출물에서 발아억제 효과가 나타났다. 경엽처리에서는 두 초종 모두 가죽나무 추출물에서 살초효과가 우수한 것으로 나타났다. Crabgrass (Digitaria sanguinalis L.), Dandelion (Taraxacum platycarpum H. DAHLST.), Annual bluegrass (Poa annua L.), Gree kyllinga (Kyllinga brevifolia var.), Red clover (Trifolium pratense L.), Annual lespedeza (Kummerowia striata (Thunb.)Schindl.), Mugwort (Artemisia capillaris Thunb.), Horseweed (Erigeron canadensis L.), Field horsetail (Equisetum arvense L.) are major weeds in turf. In this study, 23 medicinal plant extracts were tested for herbicidal activities against crabgrass and dandelion in the course of major weed in turf. In pot experiment, we implemented pre-emergence application used medicinal plant extracts showed herbicidal activity in petridish experiment. In effect, inhibition rates of germination were 61.0% at Curcuma longa extract and 62.5% at Cnidium officinale extract in crabgrass pot experiment, and 77.8% at Ailanthus altissima extract in dandelion pot experiment.

잔디 병해 방제를 위한 항균성 약용식물의 탐색

권수민,김대호,장태현,전민구,김인섭,김익휘,Kwon, Soo-Mean,Kim, Dae-Ho,Chang, Tae-Hyun,Jeon, Min-Goo,Kim, In-Seob,Kim, Ik-Hwi 한국잔디학회 2010 한국잔디학회지 Vol.24 No.2

잔디에 발생하는 주요 병해로 한지형 잔디에서 갈색잎마름병(Rhizoctonia solani AG1-1), 동전마름병(S. homoeocarpa), 피시움마름병(Pythium. spp.), 탄저병(C. graminicola)과 난지형 잔디에서 누른잎마름병(R. cerealis), 라이족토니아마름병 (R. solani AG2-2)이다. 본 연구에서는 이들 잔디 주요 병원균 6종에 대한 한약재 23종의 항진균 활성을 알아보았다. 디스크 확산법으로 항진균 활성이 있는 한약재를 선발하였고, 선발된 한약재를 대상으로 균사생육억제 효과를 실험하여 검정하였다. 항진균 활성 시험 결과, 한약재 23종 중고삼 외 11종의 시료에서 항진균 활성이 있었으며, 균사생육 억제효과를 통한 검정에서는 10 mg/10 ml(1000배) 추출물 첨가 배지에서 잔디 병원균 6종 중 1종이라도 균사 억제율이 80% 이상인 추출물은 고삼, 울금, 대황, 황련, 족두리풀로 나타났다. 특히 고삼 추출물은 실험 대상인 잔디 병원균 6종에 대해 모두 항진균 활성이 나타났고, 울금 추출물 10 mg/10 ml(1000배), 5 mg/10 ml(2000배), 2 mg/10 ml(5000배) 첨가 배지에서 S. homoeocarpa 에 대해 100% 균사 생육 억제 효과를 나타내었다. 그리고 Pythium spp. 균사 억제효과가 100%인 것은 고삼, 대황, 황련으로 나타났다. Brown patch (Rhizoctonia solani AG1-1), dollar spot (Sclerotinia homoeocarpa), pythium blight (Pythium spp.), anthracnose (Colletotrichum graminicola), yellow patch (Rhizoctonia cerealis) and Zoysia patch (Rhizoctonia solani AG2-2) are the major turfgrasses diseases in Korea. In this study, 23 medicinal plant extracts were tested for antifungal activities against turfgrass pathogenic fungi. In paper disk diffusion method, 12 medicinal plant extracts, including Sophora flavescens, showed antifungal activity. Also, in the test of antifungal activity on media contained the extracts of S. flavescens, Curcuma longa, Rheum undulatum, Coptis chinensis and Asiasarum sieboldi showed above 80% inhibitory effects on the mycelial growth in 110 mg/10 ml concentration of the extracts. S. flavescens, in particularly, showed antifungal activity against the six turfgrass pathogenic fungi. The inhibition rate of S. homoeocarpa was 100% in 10 mg/10 ml, 5 mg/10 ml and 2 mg/10 ml concentrations of C. longa extract. In case of Pythium spp., the extracts of S. flavescens, R. undulatum and C. chinensis showed 100 % inhibition rate on the test media.

한약재 추출물을 이용한 잔디밭 문제 잡초 바랭이, 민들레 방제실험

김대호(Kim, D. H.),강재영(J. Y. Kang),김인섭(I. S. Kim),전민구(M. G. Jeon),이재득(J. D. Lee),김익휘(I. H. Kim) 한국잡초학회 2012 한국잡초학회 별책(학술대회 초록집) Vol.32 No.1